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Nucleic Acids Res. 1987 February 11; 15(3): 1311–1326.
PMCID: PMC340526

Electroporation for the efficient transfection of mammalian cells with DNA.


A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.

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