PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of bmcphysBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Physiology
 
From:
Published online Jun 27, 2012. doi: 10.1186/1472-6793-12-8
An external file that holds a picture, illustration, etc.
Object name is 1472-6793-12-8-2.jpg Object name is 1472-6793-12-8-2.jpg
Figure 2
IL-1β response to acute stressor exposure in visceral and subcutaneous WAT. Effect of acute stressor exposure on the IL-1β protein content of visceral (omental) and subcutaneous (inguinal) WAT in healthy, non-obese rats. 21 week old male F344 rats were exposed to 50 inescapable 1.5 mA tail shocks (stress, n = 24) or remained in their home cages (no stress, n = 26). Rats were sacrificed immediately following stressor exposure. Approximately 0.3 g of omental and subcutaneous WAT depots were harvested, spot frozen in liquid nitrogen and processed via homogenization in a RIPA lysis buffer. IL-1β protein analysis (pg IL-1β per mg total WAT protein) was done via ELISA (R&D Systems Minneapolis, MN). Effect of depot on IL-1β concentration F(1,46) = 15.751, **P < 0.001. Effect of stress on IL-1β content: F(1,46) = 28.461, ***P < 0.0001. Depot by stress interaction: F(3,44) = 13.312, ##P < 0.001. Values represent group means + standard error of measurement. All experiment protocols were approved by the Animal Care and Use Committee of the University of Colorado at Boulder.
Articles from BMC Physiology are provided here courtesy of
BioMed Central