PCB-95 Promotes Dendritic Growth via Ryanodine Receptor–Dependent Mechanisms

doi:10.1289/ehp.1104832

Environ Health Perspect. 2012 July; 120(7): 997–1002.

Published online 2012 April 25. doi: 10.1289/ehp.1104832

PMCID: PMC3404670

Research

PCB-95 Promotes Dendritic Growth via Ryanodine Receptor–Dependent Mechanisms

Gary A. Wayman,¹ Dongren Yang,² Diptiman D. Bose,² Adam Lesiak,¹ Veronica Ledoux,³ Donald Bruun,² Isaac N. Pessah,² and Pamela J. Lein^2,³

¹Program in Neuroscience, Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, Washington, USA

²Department of Molecular Biosciences, University of California, Davis, Davis, California, USA

³Center for Research on Occupational and Environmental Toxicology, Oregon Health & Science University, Portland, Oregon, USA

Corresponding author.

Address correspondence to P.J. Lein, UC Davis School of Veterinary Medicine, Department of Molecular Biosciences, One Shields Ave., 1120 Haring Hall, Davis, CA 95616, USA. Telephone: (530) 752-1970. Fax: (530) 752-4698. E-mail: pjlein/at/ucdavis.edu

Received December 7, 2011; Accepted April 2, 2012.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

This article has been cited by other articles in PMC.

Abstract

Background: Aroclor 1254 (A1254) interferes with normal dendritic growth and plasticity in the developing rodent brain, but the mechanism(s) mediating this effect have yet to be established. Non-dioxin-like (NDL) polychlorinated biphenyls (PCBs) enhance the activity of ryanodine receptor (RyR) calcium ion (Ca²⁺) channels, which play a central role in regulating the spatiotemporal dynamics of intracellular Ca²⁺ signaling. Ca²⁺ signaling is a predominant factor in shaping dendritic arbors, but whether PCB potentiation of RyR activity influences dendritic growth is not known.

Objective: We determined whether RyR activity is required for PCB effects on dendritic growth.

Methods and Results: Golgi analysis of hippocampi from weanling rats confirmed that developmental exposure via the maternal diet to NDL PCB-95 (2,2´,3,5´6-pentachlorobiphenyl), a potent RyR potentiator, phenocopies the dendrite-promoting effects of A1254. Dendritic growth in dissociated cultures of primary hippocampal neurons and in hippocampal slice cultures is similarly enhanced by PCB-95 but not by PCB-66 (2,3,4´,4-tetrachlorobiphenyl), a congener with negligible effects on RyR activity. The dendrite-promoting effects of PCB-95 are evident at concentrations as low as 2 pM and are inhibited by either pharmacologic blockade or siRNA knockdown of RyRs.

Conclusions: Our findings demonstrate that environmentally relevant levels of NDL PCBs modulate neuronal connectivity via RyR-dependent effects on dendritic arborization. In addition, these findings identify RyR channel dysregulation as a novel mechanism contributing to dysmorphic dendritogenesis associated with heritable and environmentally triggered neurodevelopmental disorders.

Keywords: dendrites, developmental neurotoxicity, hippocampal neurons, neuronal connectivity, non-dioxin-like PCBs, ryanodine receptor

Dendritic architecture is a critical determinant of neuronal connectivity (Libersat and Duch 2004; Scott and Luo 2001). Abnormalities in dendritic shape are the most consistent pathologic correlate of behavioral deficits in heritable and environmentally triggered neurodevelopmental disorders (Bourgeron 2009; Fukuda et al. 2005; Garey 2010; Penzes et al. 2011; Svitkina et al. 2010). Polychlorinated biphenyls (PCBs) are known human developmental neurotoxicants (Carpenter 2006; Korrick and Sagiv 2008; Schantz et al. 2003), and we previously demonstrated that developmental exposure to the PCB mixture Aroclor 1254 (A1254) interferes with normal patterns of dendritic growth and plasticity in weanling rats (Lein et al. 2007; Yang et al. 2009) coincident with deficits in spatial learning and memory (Yang et al. 2009).

The dynamic structural remodeling of dendrites that occurs during development (Chen and Nedivi 2010; Cline 2001) is driven in large part by calcium ion (Ca²⁺)-dependent signaling pathways triggered by NMDA (N-methyl-d-aspartate) receptor activation and extrinsic cues such as neurotrophins (Lohmann and Wong 2005; Wayman et al. 2008). Non-dioxin-like (NDL) PCBs increase intracellular Ca²⁺ in neurons via several mechanisms, the most sensitive of which is potentiation of ryanodine receptor (RyR) activity (Pessah et al. 2010). RyRs are ion channels in the endoplasmic reticulum (ER) that regulate Ca²⁺ release from the ER and modulate the gating response and signal gain of plasma membrane ion channels, including the NMDA receptor (Pessah et al. 2010). RyR activity determines the amplitude and spatiotemporal patterns of intracellular Ca²⁺ fluxes (Berridge 2006). NDL PCBs interact with RyRs to dramatically alter their sensitivity to physiological modulators (Wong et al. 1997).

A1254 consists primarily of NDL PCBs (Kostyniak et al. 2005) with varying RyR-sensitizing potencies (Pessah et al. 2006), suggesting that effects of developmental A1254 exposure on dendritic arborization are mediated by RyR-dependent mechanisms. In support of this hypothesis, we demonstrated that A1254 interference with dendritic growth and plasticity in cerebellar Purkinje cells correlated with altered RyR expression and activity in the cerebellum (Yang et al. 2009) and that nanomolar concentrations of environmentally relevant PCB-95 (2,2´,3,5´,6-pentachlorobiphenyl), a congener that potently activates RyRs (Pessah et al. 2006), enhanced dendritic growth in primary cultures of neocortical neurons (Yang et al. 2009). Here, we provide evidence of a causal relationship between PCB-95–induced dendritic growth and RyR activation in hippocampal neurons.

Methods

A complete listing of reagents is provided in Supplemental Material, p. 3 (http://dx.doi.org/10.1289/ehp.1104832). Animals were treated humanely and with regard for alleviation of suffering according to protocols approved by the Institutional Animal Care and Use Committees of the Johns Hopkins University; Oregon Health & Science University; University of California, Davis; and Washington State University, Pullman.

In vivo PCB exposures. Adult Long Evans rats were purchased from Charles River Laboratories (Hollister, CA), and husbandry practices were as previously described (Yang et al. 2009). Dams were dosed with A1254 (1 or 6 mg/kg/day), PCB-95 (0.1, 1, or 6 mg/kg/day), or vehicle (peanut oil) as previously described (Yang et al. 2009) beginning 14 days before breeding and continuing until postnatal day (PD) 21. Dams delivered 10–15 pups (n = 11 dams per treatment group). By PD2, litters were culled to 10 pups. Pups were weaned on PD21 and euthanized at PD31 (A1254 studies) or PD38 (PCB-95 studies). Exposure to either A1254 or PCB-95 in the maternal diet throughout gestation and lactation did not affect the body weight of pregnant and lactating dams, litter size, sex ratios, or growth rates of the pups.

Cell culture. Hippocampal neurons (10⁵ cells/cm²) were dissociated from PD1 Sprague-Dawley rats (Charles River Laboratories) and cultured in Neurobasal-A (Invitrogen, Carlsbad, CA) supplemented with B27 (Invitrogen) as described previously (Wayman et al. 2006). At 5–6 days in vitro (DIV), cultures were transfected with plasmid-encoding microtubule-associated-protein-2B (MAP2B) fused to enhanced green fluorescent protein (EGFP), which selectively labels the somatodendritic domain (Wayman et al. 2006), using Lipofectamine-2000 (Invitrogen) according to the manufacturer’s protocol. At 7 DIV, cultures were treated for 48 hr with vehicle (DMSO; 1:1,000 dilution), PCB-95 (2 fM–2 μM), or PCB-66 (2,3,4´,4-tetrachlorobiphenyl; 200 nM) diluted from 1,000× stocks. Hippocampal slices from PD5 Sprague-Dawley rats were cultured for 3 days as described (Lein et al. 2011). To visualize dendritic arbors, slice cultures were biolistically transfected with plasmid-encoding tomato fluorescent protein (TFP), which fills the entire cell, using a Helios Gene Gun (BioRad, Hercules, CA), according to the manufacturer’s protocol. DNA amounts, transfection reagent amounts, and transfection duration were optimized to minimize toxicity and maximize transfection efficiency. Following transfection, slices were allowed to recover for 24 hr before exposure to PCB-95 for 48 hr.

Dendritic analyses. Dendritic arbors of pyramidal neurons in the CA1 hippocampus of weanling rats were Golgi stained and quantified by Sholl analysis (Lein et al. 2007). Sholl data were evaluated using the Wilcoxon rank-sign test applying a conservative alpha level based on the number of measurements (Dawson and Trapp 2004). Soma size was analyzed using Image J version 1.44p with the Neuron J plug-in version 1.42 to trace neurons (Meijering et al. 2004), and significant differences were determined using Student t-test with significance set at p < 0.05. Dendritic morphology in dissociated hippocampal cultures or hippocampal slice cultures was quantified from digital images of green fluorescent protein–positive (GFP⁺) or TFP⁺ neurons, respectively, using NeuronJ (Meijering et al. 2004). Dendritic length and number of dendritic termini per neuron were analyzed by one-way analysis of variation with significance set at p < 0.05. Differences between treatment groups were identified by post hoc Tukey’s test. All morphometric experiments were replicated in cultures derived from at least three independent dissections.

RyR expression. RyR expression in lysates (25 µL/well) of 2-, 7-, and 12-DIV dissociated cultures or whole membrane (100,000 × g) fractions prepared from adult (2–4 month old) rat hippocampi were size-separated on SDS-PAGE (4–20%) and Western blotting was performed using anti-RyR1 antibody 34C (1:500; University of Iowa Hybridoma Bank, Iowa City, IA) or anti-RyR2 antibody C3-33 (1:500; generous gift of G. Meissner, University of North Carolina–Chapel Hill) as previously described (Yang et al. 2009). Blots were re-probed with a monoclonal antibody (Sigma-Aldrich Corp., St. Louis, MO) that recognizes α-tubulin to which RyR bands were normalized. To localize RyR in hippocampal neurons, dissociated cultures were fixed in 4% paraformaldehyde at 21 DIV, permeabilized with 0.05% Triton X-100, blocked in PBS (phosphate buffered solution) containing 2% glycerol, 0.05 M NH₄Cl (ammonium chloride), 5% FBS (fetal bovine serum), 2% goat serum and then incubated with fluorochrome-tagged phalloidin (Molecular Probes, Invitrogen; 1:200) and antibody C3-33 (1:100), which recognizes all three RyR isoforms at that dilution (Meissner 2002). Antibody–antigen complexes were visualized using fluorochrome-conjugated secondary antibodies (Molecular Probes, Invitrogen). Z-stack images were obtained using a Delta Vision Core Imaging System (Applied Precision, LLC, Seattle, WA) and deconvoluted using Imaris software, version 6.2 (Bitplane, South Windsor, CT), as previously described (Chen et al. 2010).

Results

PCBs enhance dendritic growth in the developing hippocampus. A1254 is a commercial PCB mixture that includes many of the congeners associated with human exposures (Kodavanti et al. 2001; Kostyniak et al. 2005). We have previously showed that exposure to A1254 at 1 and 6 mg/kg/day in the maternal diet throughout gestation and lactation resulted in PCB levels in the brains of weanling rats comparable to those reported in human brain tissue and also promoted dendritic growth in cerebellar Purkinje cells and neocortical pyramidal neurons (Yang et al. 2009). Using this dosing regimen, we examined the effects of developmental A1254 exposure on hippocampal pyramidal neurons at PD31. Representative camera lucida drawings of the basilar dendritic arbor of Golgi-impregnated CA1 pyramidal neurons demonstrate that A1254 significantly increases dendritic complexity (Figure 1A). Sholl analysis indicates that dendritic arborization was significantly increased relative to vehicle controls by 25% and 29% in the 1 and 6 mg/kg/day A1254 treatment groups, respectively (Figure 1B).

Figure 1

PCBs enhance dendritic growth in vivo. (A) Representative camera lucida images and (B) Sholl analyses of the basilar dendritic arbor of Golgi-stained CA1 pyramidal neurons from PD31 weanling rats exposed throughout gestation and lactation to vehicle or (more ...)

To determine the potential contribution of RyR-active NDL PCBs to the dendrite-promoting activity of A1254, we examined dendritic arborization in pyramidal neurons of weanling rats exposed throughout gestation and lactation to PCB-95 in the maternal diet at 0.1, 1.0, or 6.0 mg/kg/day. PCB-95 is the most potent RyR activator yet identified among the NDL congeners (Pessah et al. 2006). Sholl analyses of Golgi-stained pyramidal neurons in PD38 weanlings indicated that developmental PCB-95 exposure increased dendritic growth by 20% and 21% in the 0.1 and 1.0 mg/kg/day PCB-95 treatment groups, respectively, but that dendritic growth in the 6 mg/kg/day PCB-95 treatment group did not differ significantly from vehicle controls (Figure 1C). Consistent with previous studies (Lein et al. 2007), soma size of pyramidal neurons was not affected by developmental exposure to either A1254 or PCB-95 (data not shown).

RyR expression in cultured hippocampal neurons. Mechanistic studies of RyR involvement in the dendrite-promoting activity of PCB-95 were studied with primary cultured hippocampal neurons. All three RyR isoforms are expressed in the brain although their relative expression varies as a function of developmental age and brain region (Berridge 2006); therefore, we first determined patterns of RyR expression in cultured hippocampal neurons by Western blotting. Monoclonal antibody (mAb) 34C or C3-33 at concentrations that selectively bind to RyR1 and RyR3 (Airey et al. 1990) or to RyR2 (Lai et al. 1992), respectively labeled 565 and 557 kDa bands, in 7- and 12-DIV lysates, whereas only RyR1 was detectable at 2 DIV (Figure 2A). A band with a lower molecular mass corresponding to RyR3 (545 kDa) was not detected by mAb 34C from cultures at any time point examined. This is consistent with reports that RyR3 accounts for about 2% of the total RyR protein in the brain (Kim et al. 2007; Murayama and Ogawa 1996) and in hippocampal cultures not stimulated with BDNF (brain-derived neurotrophic factor) (Adasme et al. 2011). These data verify that both RyR1 and RyR2 were expressed in hippocampal neurons during the period of most robust dendritic growth in these cultures (Wayman et al. 2006).

Figure 2

RyR expression in cultured hippocampal neurons. (A) Representative blots and densitometric analyses of membrane fractions from rat hippocampal cultures harvested at 2, 7, and 12 DIV, and probed with RyR1- or RyR2-selective monoclonal antibodies and a (more ...)

Immunocytochemical localization of RyRs in dissociated hippocampal neuronal cell cultures co-labeled with the F-actin label phalloidin to identify actin-rich dendritic spines confirmed RyR immunoreactive puncta throughout the dendritic shafts of cultured hippocampal neurons (Figure 2B).

PCB-induced dendritic growth is mediated by RyR-dependent mechanisms. To further test the validity of our in vitro model system, we determined whether the dendrite-promoting activity of PCB-95 is recapitulated in cultured hippocampal neurons. Dendritic arbors of individual neurons in high-density neuron-glia co-cultures were visualized by expressing a MAP2B-EGFP construct under the control of the CAG promoter, which exhibits neuron-specific expression (Wayman et al. 2006). Expression of MAP2B-EGFP is restricted to the somatodendritic compartment in cultured hippocampal neurons and does not alter their intrinsic dendritic growth patterns (Wayman et al. 2006). Exposure to PCB-95 between 7–9 DIV significantly enhanced dendritic growth of hippocampal neurons in these cultures as observed at 9 DIV (Figure 3A). Initial concentration range-finding studies indicated increased dendritic length and branching at concentrations as low as 2 pM; however, these effects were no longer evident at concentrations ≥ 2 μM (Figure 3B). Experiments to refine the upper range of the concentration–effect curve indicates dendritic responses of comparable magnitude at concentrations of 2–200 nM; at concentrations of > 200 nM, PCB-95 no longer elicited increased dendritic growth (data not shown). Subsequent mechanistic studies used the maximally effective concentration of 200 nM PCB-95.

Figure 3

PCB-95 enhances dendritic growth in cultured hippocampal neurons. (A) Representative photomicrographs of 9-DIV hippocampal neurons expressing MAP2B-EGFP after 48-hr exposure to vehicle (control) or PCB-95 (2 nM). (B) Concentration-dependent effect of (more ...)

If PCBs (200 nM) enhance dendritic growth by potentiating RyR activation, then congeners with differential effects on RyR activation should differentially influence dendritic growth. To test this, we compared dendritic growth in hippocampal cultures exposed to PCB-95 vs. PCB-66, which exhibit potent vs. negligible effects on RyR activity, respectively (Pessah et al. 2006). Exposure to PCB-95 (200 nM) during 7–9 DIV significantly increased total dendritic length and branching as observed at 9 DIV (Figure 4A, B). In contrast, dendritic length and branching in hippocampal neurons exposed to PCB-66 (200 nM) for the same period of time did not differ from that observed in vehicle controls (Figure 4A,B). Moreover, block of RyR channels by FLA365 (4-(2-aminopropyl)-3,5-dichloro-N,N-dimethylaniline) (Chiesi et al. 1988; Mack et al. 1992) suppressed the dendrite-promoting activity of PCB-95 but had no effect on dendritic length or branching in control cultures not exposed to PCB-95 (Figure 4A,B).

Figure 4

In vitro PCB-induced dendritic growth requires RyR activity. PCB-95 (200 nM), but not PCB-66 (200 nM), increased dendritic length (A) and branching (B) in primary cultures of dissociated hippocampal neurons (9 DIV), and this effect was blocked by the (more ...)

The physiological relevance of these observations was confirmed using hippocampal slice cultures from PD5 rats biolistically labeled with TFP at 3 DIV and then exposed to PCBs from 5–7 DIV. PCB-95, but not PCB-66, significantly increased the number of dendritic termini per neuron in TFP⁺ neurons in a concentration-dependent manner, and this effect was blocked by FLA365 (Figure 4C).

PCB-95 sensitizes both RyR1 and RyR2 (Wong and Pessah 1996). Therefore, to assess the relative contributions of RyR1 and RyR2 in PCB-induced dendritic growth, we expressed siRNA (small interfering RNA) constructs that selectively suppress each RyR isoform [see Supplemental Material, Table S1 (http://dx.doi.org/10.1289/ehp.1104832)]. Experiments in HEK293 cells (see Supplemental Material, p. 4) confirmed that each siRNA blocked expression of its target RyR isoform without altering expression of the other non-target isoform (see Supplemental Material, Figure S1). Expression in hippocampal neurons of siRNA against either RyR1 or RyR2 suppressed PCB-95 stimulation of dendritic length and branching but had no effect on basal dendritic growth (Figure 5). In contrast, expression of control siRNA had no effect on either PCB-95–induced or basal dendritic growth (Figure 5).

Figure 5

RyR-specific siRNA blocks PCB-induced dendritic growth in vitro. (A) Representative photomicrographs of 9-DIV hippocampal neurons transfected with MAP2B-EGFP alone or in combination with RyR siRNA before 48-hr treatment with vehicle or PCB-95 (200 nM). (more ...)

Discussion

We previously reported that developmental A1254 exposure promotes dendritic growth in cerebellar Purkinje cells and cortical pyramidal neurons of weanling rats and that nanomolar concentrations of PCB-95 promote dendritic growth in cultured neocortical neurons (Yang et al. 2009). Here, we extend these observations to show that a) developmental exposure to the NDL PCB-95 in the maternal diet phenocopies the effect of developmental A1254 exposure on dendritic arborization in the developing hippocampus, b) PCB-95 promotes dendritic growth in cultured hippocampal neurons at picomolar to nanomolar concentrations, and c) the dendrite-promoting activity of PCB-95 requires RyR activity.

As a first test of the cause–effect relationship between PCB-enhanced RyR activity and PCB-induced dendritic growth, we compared dendritic growth in cultured hippocampal neurons exposed to PCB-95 vs. PCB-66. PCB-95 is a tri-ortho substituted congener that potently sensitizes the RyR channel to physiological and pharmacological activators (Wong and Pessah 1996) and preferentially stabilizes the RyR in its full open conformation (Samso et al. 2009). PCB-66 has a single ortho-chlorine substitution, and despite similar physicochemical properties to PCB-95, has negligible influence on RyR activity (Pessah et al. 2006). In cultured hippocampal neurons, PCB-95 promotes dendritic growth, whereas PCB-66 has no effect at the concentrations tested. As a second test, blocking RyR function by either pharmacological antagonism or siRNA knockdown prevented PCB-95–induced dendritic growth. Interestingly, RyR1 siRNA and RyR2 siRNA were equally effective in blocking the dendrite-promoting activity of PCB-95. Studies in HEK cells confirmed that each siRNA was specific for its target mRNA and did not cross-react with non-target RyR mRNA. The native activities of both RyR isoforms are therefore required for the dendrite-promoting activity of PCB-95, and interference with either isoform is sufficient to prevent the influence of RyR-active PCBs, without altering basal dendritic growth or cell viability. The biology underlying the dual requirement for RyR1 and RyR2 in PCB-induced dendritic growth remains to be determined. We see three possible explanations.

One possibility is that each isoform regulates a complementary but distinct profile of downstream effectors necessary for dendritic growth. For example, both transcription- and translation-dependent pathways mediate activity-dependent dendritic growth (Schratt et al. 2004; Tsokas et al. 2005; Vickers et al. 2005; Wayman et al. 2008) and activity-dependent spine formation requires turning on signaling pathways that promote spine formation coincident with turning off signaling pathways that inhibit spine formation (Impey et al. 2010; Saneyoshi et al. 2010).

A second possibility is that activation of spatially segregated RyR1 and RyR2 channels creates Ca²⁺ microdomains within the soma and the dendritic processes and terminals (Berridge 2006) whose coincident activation is necessary for enhancing activity-dependent dendritic growth. As described in the companion paper (Wayman et al. 2012), PCB-95 enhances spontaneous Ca²⁺ oscillations within the soma and distal dendrites of the same neuron, and these effects are blocked by ryanodine. Collectively, these data indicate that NDL PCBs mediate the gain of RyR function that promotes dendritic growth, and they suggest a role for RyR in normal activity-dependent dendritic growth.

The human health relevance of these in vitro mechanistic studies is supported by our observations that developmental PCB exposure similarly promotes dendritic growth in the developing brains of weanling rats. Using an exposure paradigm relevant to human PCB exposures in terms of route of exposure, dose level, and dose duration (Yang et al. 2009), we observed that A1254 increases dendritic growth in pyramidal neurons of the CA1 hippocampus. A1254 is composed predominantly of NDL PCB congeners with RyR activity (Kostyniak et al. 2005), consistent with our in vitro data establishing that PCBs promote dendritic growth via RyR-dependent mechanisms. The proposal that NDL PCBs contribute to the dendrite-promoting activity of A1254 is strengthened by the observation that developmental exposure to PCB-95 in the maternal diet similarly stimulates dendritic growth in vivo. Interestingly, over the dose ranges tested in this study, PCB-95, but not A1254, exhibited an inverted dose–response relationship. Although we cannot rule out the possibility that nonlinearity would also be observed with A1254 at doses > 6 mg/kg/day, the different dose–response relationships observed for A1254 vs. PCB-95 may reflect the fact that A1254 is a mixture of DL and NDL PCBs with varying RyR potency (Kostyniak et al. 2005). Alternatively, up-regulation of cytochrome P450 enzymes by DL PCBs in A1254 could result in different toxicokinetics of NDL PCBs in A1254- vs. PCB-95–exposed animals (Gauger et al. 2007; Giera et al. 2011).

The nonlinear dose response of developmental PCB-95 exposure on dendritic growth in vivo is recapitulated in vitro. The loss of dendrite-promoting activity in vitro at PCB-95 concentrations > 2 μM is likely not due to decreased cell viability (Howard et al. 2003). A plausible explanation is that chronic RyR sensitization alters RyR expression and/or activity, in an inverted concentration-related manner, which is perhaps due to local production of reactive oxygen species (ROS) by PCBs (Fonnum et al. 2006). Microsomal RyR complexes possess a small number of highly reactive cysteines that confer tight regulation of Ca²⁺ channel activity in response to changing transmembrane redox potential (Feng et al. 2000; Liu et al. 1994). Thus the sensitizing and desensitizing influence of RyR-active PCBs on Ca²⁺ signaling may be strongly influenced by the level of ROS production (Pessah et al. 2002). A parallel mechanism has been demonstrated in which moderate increases in Ca²⁺ promote dendritic growth, whereas large increases cause dendritic retraction (Lohmann and Wong 2005; Segal et al. 2000). Thus, when increasing the concentration of PCB-95 from 200 nM to 2 μM, intracellular Ca²⁺ concentrations may cross the threshold from concentrations that promote dendritic growth to those that cause dendritic retraction.

A third possibility is suggested by the report that transfection of cortical neurons with constitutively active CaMKIV (Ca²⁺/calmodulin-dependent protein kinase IV) promotes dendritic growth, whereas expression of constitutively active CaMKII (Ca²⁺/calmodulin-dependent protein kinase II) inhibits dendritic growth (Redmond et al. 2002). Perhaps at higher concentrations that do not promote dendritic growth, PCBs are preferentially activating Ca²⁺-dependent signaling molecules that inhibit dendritic growth. Distinguishing between these possibilities is the focus of future studies.

These data linking a direct molecular effect of NDL PCBs (sensitized RyR activity) to disruption of a specific neurodevelopmental event (dendritic arborization) have significant implications for understanding how PCBs interfere with normal neurodevelopment in the human brain. Structural aberrations in the dendritic arbors of central neurons are thought to contribute to clinical manifestations of diverse heritable and environmentally induced neurodevelopmental disorders in humans (Bourgeron 2009; Fukuda et al. 2005; Garey 2010; Penzes et al. 2011; Svitkina et al. 2010). Consistent with this, developmental A1254 exposure altered dendritic arborization in weanling rats coincident with performance deficits in the Morris swim task (Yang et al. 2009) and exposure to PCB-95 on gestation days 10–16 altered behavior in adult rats (Schantz et al. 1997). NDL PCB congeners with the highest activity towards RyRs, including PCB-95, collectively represent 40–50% of the total PCBs currently found in environmental and biotic samples, and their net effects are likely to be additive (Pessah et al. 2006). However, even low levels of PCB exposure might adversely influence neuronal connectivity in the developing brain of genetically susceptible individuals. Mutations in RYR (ryanodine receptor) genes have been linked to environmentally triggered disorders in humans including malignant hyperthermia (Pessah et al. 1996), cardiac arrhythmias (Wehrens et al. 2005), and sudden death (Laitinen et al. 2004). Recent studies demonstrate that specific RYR mutations confer sex– and gene–dose-dependent susceptibility to pharmacological (halogenated anesthetic) and environmental (heat) stressors that trigger malignant hyperthermia and muscle damage in otherwise asymptomatic individuals (Barrientos et al. 2012; Yuen et al. 2012). Importantly, PCB-95 is significantly more potent and efficacious in disrupting cation regulation of mutant R615C-RYR1 compared with wild type RyR1 (Ta and Pessah 2007). Considered together, these observations identify PCBs, and in particular NDL PCBs with high RyR activity, as candidate environmental risk factors in neurodevelopmental disorders and provide important new clues about the possible role of RyRs in contributing to heritable and environmentally triggered neurodevelopmental deficits.

Supplemental Material

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Acknowledgments

We thank G. Meissner for the generous gift of anti-RyR2 antibody C3-33 and Y. Chen for providing the photomicrograph used in Figure 2.

Footnotes

This work was supported by grants from the National Institutes of Health (R01 ES014901, R01 ES017425, P42 ES04699, R01 MH086032, P01 ES011269, and T32 ES007060); the U.S. Environmental Protection Agency (R833292 and R829388); the Hope for Depression Research Foundation; and the J.B. Johnson Foundation.

The authors declare they have no actual or potential competing financial interests.

References

Adasme T, Haeger P, Paula-Lima AC, Espinoza I, Casas-Alarcon MM, Carrasco MA, et al. Involvement of ryanodine receptors in neurotrophin-induced hippocampal synaptic plasticity and spatial memory formation. Proc Natl Acad Sci U S A. 2011;108(7):3029–3034. [PubMed]
Airey JA, Beck CF, Murakami K, Tanksley SJ, Deerinck TJ, Ellisman MH, et al. Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle. J Biol Chem. 1990;265(24):14187–14194. [PubMed]
Barrientos GC, Feng W, Truong K, Matthaei KI, Yang T, Allen PD, et al. Gene dose influences cellular and calcium channel dysregulation in heterozygous and homozygous T4826I–RYR1 malignant hyperthermia-susceptible muscle. J Biol Chem. 2012;287(4):2863–2876. [PubMed]
Berridge MJ. Calcium microdomains: organization and function. Cell Calcium. 2006;40(5–6):405–412. [PubMed]
Bourgeron T. A synaptic trek to autism. Curr Opin Neurobiol. 2009;19(2):231–234. [PubMed]
Carpenter DO. Polychlorinated biphenyls (PCBs): routes of exposure and effects on human health. Rev Environ Health. 2006;21(1):1–23. [PubMed]
Chen JL, Nedivi E. Neuronal structural remodeling: is it all about access? Curr Opin Neurobiol. 2010;20(5):557–562. [PMC free article] [PubMed]
Chen Y, Tassone F, Berman RF, Hagerman PJ, Hagerman RJ, Willemsen R, et al. Murine hippocampal neurons expressing Fmr1 gene premutations show early developmental deficits and late degeneration. Hum Mol Genet. 2010;19(1):196–208. [PMC free article] [PubMed]
Chiesi M, Schwaller R, Calviello G. Inhibition of rapid Ca-release from isolated skeletal and cardiac sarcoplasmic reticulum (SR) membranes. Biochem Biophys Res Commun. 1988;154(1):1–8. [PubMed]
Cline HT. Dendritic arbor development and synaptogenesis. Curr Opin Neurobiol. 2001;11(1):118–126. [PubMed]
Dawson B, Trapp RG. 4th ed. New York:Lange Medical Books. 2004. Basic and Clinical Biostatistics.
Feng W, Liu G, Allen PD, Pessah IN. Transmembrane redox sensor of ryanodine receptor complex. J Biol Chem. 2000;275(46):35902–35907. [PubMed]
Fonnum F, Mariussen E, Reistad T. Molecular mechanisms involved in the toxic effects of polychlorinated biphenyls (PCBs) and brominated flame retardants (BFRs). J Toxicol Environ Health A. 2006;69(1–2):21–35. [PubMed]
Fukuda T, Itoh M, Ichikawa T, Washiyama K, Goto Y. Delayed maturation of neuronal architecture and synaptogenesis in cerebral cortex of Mecp2-deficient mice. J Neuropathol Exp Neurol. 2005;64(6):537–544. [PubMed]
Garey L. When cortical development goes wrong: schizophrenia as a neurodevelopmental disease of microcircuits. J Anat. 2010;217(4):324–333. [PubMed]
Gauger KJ, Giera S, Sharlin DS, Bansal R, Iannacone E, Zoeller RT. Polychlorinated biphenyls 105 and 118 form thyroid hormone receptor agonists after cytochrome P4501A1 activation in rat pituitary GH3 cells. Environ Health Perspect. 2007;115:1623–1630. [PMC free article] [PubMed]
Giera S, Bansal R, Ortiz-Toro TM, Taub DG, Zoeller RT. Individual polychlorinated biphenyl (PCB) congeners produce tissue- and gene-specific effects on thyroid hormone signaling during development. Endocrinology. 2011;152(7):2909–2919. [PubMed]
Howard AS, Fitzpatrick R, Pessah I, Kostyniak P, Lein PJ. Polychlorinated biphenyls induce caspase-dependent cell death in cultured embryonic rat hippocampal but not cortical neurons via activation of the ryanodine receptor. Toxicol Appl Pharmacol. 2003;190(1):72–86. [PubMed]
Impey S, Davare M, Lasiek A, Fortin D, Ando H, Varlamova O, et al. An activity-induced microRNA controls dendritic spine formation by regulating Rac1-PAK signaling. Mol Cell Neurosci. 2010;43(1):146–156. [PMC free article] [PubMed]
Kim S, Yun HM, Baik JH, Chung KC, Nah SY, Rhim H. Functional interaction of neuronal Cav1.3 L-type calcium channel with ryanodine receptor type 2 in the rat hippocampus. J Biol Chem. 2007;282(45):32877–32889. [PubMed]
Kodavanti PR, Kannan N, Yamashita N, Derr-Yellin EC, Ward TR, Burgin DE, et al. Differential effects of two lots of Aroclor 1254: congener-specific analysis and neurochemical end points. Environ Health Perspect. 2001;109:1153–1161. [PMC free article] [PubMed]
Korrick SA, Sagiv SK. Polychlorinated biphenyls, organochlorine pesticides and neurodevelopment. Curr Opin Pediatr. 2008;20(2):198–204. [PubMed]
Kostyniak PJ, Hansen LG, Widholm JJ, Fitzpatrick RD, Olson JR, Helferich JL, et al. Formulation and characterization of an experimental PCB mixture designed to mimic human exposure from contaminated fish. Toxicol Sci. 2005;88(2):400–411. [PubMed]
Lai FA, Dent M, Wickenden C, Xu L, Kumari G, Misra M, et al. Expression of a cardiac Ca²⁺-release channel isoform in mammalian brain. Biochem J. 1992;288(Pt 2):553–564. [PubMed]
Laitinen PJ, Swan H, Piippo K, Viitasalo M, Toivonen L, Kontula K. 2004. Genes, exercise and sudden death: molecular basis of familial catecholaminergic polymorphic ventricular tachycardia. Ann Med 36 Suppl 1:81–86.86. [PubMed]
Lein PJ, Barnhart CD, Pessah IN. Acute hippocampal slice preparation and hippocampal slice cultures. Methods Mol Biol. 2011;758:115–134. [PMC free article] [PubMed]
Lein PJ, Yang D, Bachstetter AD, Tilson HA, Harry GJ, Mervis RF, et al. Ontogenetic alterations in molecular and structural correlates of dendritic growth after developmental exposure to polychlorinated biphenyls. Environ Health Perspect. 2007;115:556–563. [PMC free article] [PubMed]
Libersat F, Duch C. Mechanisms of dendritic maturation. Mol Neurobiol. 2004;29(3):303–320. [PubMed]
Liu G, Abramson JJ, Zable AC, Pessah IN. Direct evidence for the existence and functional role of hyperreactive sulfhydryls on the ryanodine receptor–triadin complex selectively labeled by the coumarin maleimide 7-diethylamino-3-(4´-maleimidylphenyl)-4-methylcoumarin. Mol Pharmacol. 1994;45(2):189–200. [PubMed]
Lohmann C, Wong RO. Regulation of dendritic growth and plasticity by local and global calcium dynamics. Cell Calcium. 2005;37(5):403–409. [PubMed]
Mack WM, Zimanyi I, Pessah IN. Discrimination of multiple binding sites for antagonists of the calcium release channel complex of skeletal and cardiac sarcoplasmic reticulum. J Pharmacol Exp Ther. 1992;262(3):1028–1037. [PubMed]
Meijering E, Jacob M, Sarria JC, Steiner P, Hirling H, Unser M. Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images. Cytometry A. 2004;58(2):167–176. [PubMed]
Meissner G. Regulation of mammalian ryanodine receptors. Front Biosci. 2002;7:d2072–d2080. [PubMed]
Murayama T, Ogawa Y. Properties of Ryr3 ryanodine receptor isoform in mammalian brain. J Biol Chem. 1996;271(9):5079–5084. [PubMed]
Penzes P, Cahill ME, Jones KA, VanLeeuwen JE, Woolfrey KM. Dendritic spine pathology in neuropsychiatric disorders. Nat Neurosci. 2011;14(3):285–293. [PMC free article] [PubMed]
Pessah IN, Cherednichenko G, Lein PJ. Minding the calcium store: ryanodine receptor activation as a convergent mechanism of PCB toxicity. Pharmacol Ther. 2010;125(2):260–285. [PMC free article] [PubMed]
Pessah IN, Hansen LG, Albertson TE, Garner CE, Ta TA, Do Z, et al. Structure–activity relationship for noncoplanar polychlorinated biphenyl congeners toward the ryanodine receptor–Ca²⁺ channel complex type 1 (RyR1). Chem Res Toxicol. 2006;19(1):92–101. [PubMed]
Pessah IN, Kim KH, Feng W. Redox sensing properties of the ryanodine receptor complex. Front Biosci. 2002;7:a72–a79. [PubMed]
Pessah IN, Lynch C, Gronert GA. Complex pharmacology of malignant hyperthermia. Anesthesiology. 1996;84:1275–1279. [PubMed]
Redmond L, Kashani AH, Ghosh A. Calcium regulation of dendritic growth via CaM kinase IV and CREB-mediated transcription. Neuron. 2002;34(6):999–1010. [PubMed]
Samso M, Feng W, Pessah IN, Allen PD. 2009. Coordinated movement of cytoplasmic and transmembrane domains of RyR1 upon gating. PLoS biology 74e1000085; doi:[Online 14 April 2009] doi: 10.1371/journal.pbio.1000085. [PMC free article] [PubMed] [Cross Ref]
Saneyoshi T, Fortin DA, Soderling TR. Regulation of spine and synapse formation by activity-dependent intracellular signaling pathways. Curr Opin Neurobiol. 2010;20(1):108–115. [PMC free article] [PubMed]
Schantz SL, Seo BW, Wong PW, Pessah IN. Long-term effects of developmental exposure to 2,2´,3,5´,6- pentachlorobiphenyl (PCB 95) on locomotor activity, spatial learning and memory and brain ryanodine binding. Neurotoxicology. 1997;18(2):457–467. [PubMed]
Schantz SL, Widholm JJ, Rice DC. Effects of PCB exposure on neuropsychological function in children. Environ Health Perspect. 2003;111:357–376. [PMC free article] [PubMed]
Schratt GM, Nigh EA, Chen WG, Hu L, Greenberg ME. BDNF regulates the translation of a select group of mRNAs by a mammalian target of rapamycin-phosphatidylinositol 3-kinase-dependent pathway during neuronal development. J Neurosci. 2004;24(33):7366–7377. [PubMed]
Scott EK, Luo L. How do dendrites take their shape? Nat Neurosci. 2001;4(4):359–365. [PubMed]
Segal M, Korkotian E, Murphy DD. Dendritic spine formation and pruning: common cellular mechanisms? Trends Neurosci. 2000;23(2):53–57. [PubMed]
Svitkina T, Lin WH, Webb DJ, Yasuda R, Wayman GA, Van Aelst L, et al. Regulation of the postsynaptic cytoskeleton: roles in development, plasticity, and disorders. J Neurosci. 2010;30(45):14937–14942. [PMC free article] [PubMed]
Ta TA, Pessah IN. Ryanodine receptor type 1 (RyR1) possessing malignant hyperthermia mutation R615C exhibits heightened sensitivity to dysregulation by non-coplanar 2,2´,3,5´,6-pentachlorobiphenyl (PCB 95). Neurotoxicology. 2007;28:770–779. [PMC free article] [PubMed]
Tsokas P, Grace EA, Chan P, Ma T, Sealfon SC, Iyengar R, et al. Local protein synthesis mediates a rapid increase in dendritic elongation factor 1A after induction of late long-term potentiation. J Neurosci. 2005;25(24):5833–5843. [PubMed]
Vickers CA, Dickson KS, Wyllie DJ. Induction and maintenance of late-phase long-term potentiation in isolated dendrites of rat hippocampal CA1 pyramidal neurones. J Physiol (Lond) 2005;568:803–813. [PubMed]
Wayman GA, Bose DD, Yang D, Lesiak A, Bruun D, Impey S, et al. PCB-95 modulates the calcium-dependent signaling pathway responsible for activity-dependent dendritic growth. Environ Health Perspect. 2012;120:1003–1009. [PMC free article] [PubMed]
Wayman GA, Impey S, Marks D, Saneyoshi T, Grant WF, Derkach V, et al. Activity-dependent dendritic arborization mediated by CaM-kinase I activation and enhanced CREB-dependent transcription of Wnt-2. Neuron. 2006;50(6):897–909. [PubMed]
Wayman GA, Lee YS, Tokumitsu H, Silva AJ, Soderling TR. Calmodulin-kinases: modulators of neuronal development and plasticity. Neuron. 2008;59(6):914–931. [PMC free article] [PubMed]
Wehrens XH, Lehnart SE, Marks AR. Intracellular calcium release and cardiac disease. Annu Rev Physiol. 2005;67:69–98. [PubMed]
Wong PW, Brackney WR, Pessah IN. Ortho-substituted polychlorinated biphenyls alter microsomal calcium transport by direct interaction with ryanodine receptors of mammalian brain. J Biol Chem. 1997;272(24):15145–15153. [PubMed]
Wong PW, Pessah IN. Ortho-substituted polychlorinated biphenyls alter calcium regulation by a ryanodine receptor-mediated mechanism: structural specificity toward skeletal- and cardiac-type microsomal calcium release channels. Mol Pharmacol. 1996;49(4):740–751. [PubMed]
Yang D, Kim KH, Phimister A, Bachstetter AD, Ward TR, Stackman RW, et al. Developmental exposure to polychlorinated biphenyls interferes with experience-dependent dendritic plasticity and ryanodine receptor expression in weanling rats. Environ Health Perspect. 2009;117:426–435. [PMC free article] [PubMed]
Yuen B, Boncompagni S, Feng W, Yang T, Lopez JR, Matthaei KI, et al. Mice expressing T4826I-RYR1 are viable but exhibit sex- and genotype-dependent susceptibility to malignant hyperthermia and muscle damage. Faseb J. 2012;26(3):1311–1322. [PubMed]

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