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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Biochemistry. Author manuscript; available in PMC 2012 July 17.
Published in final edited form as:
PMCID: PMC3398506

The physical basis of FGFR3 response to fgf1 and fgf2


Fibroblast growth factors (fgfs) play important roles in embryonic development and in adult life, by controlling cell proliferation, differentiation, and migration. There are 18 known fgfs which activate four fibroblast growth factor receptors (FGFRs), with different isoforms due to alternative splicing. The physical basis behind the specificity of the biological responses mediated by different fgf-FGFR pairs is currently unknown. To gain insight into the specificity of FGFR3c, a membrane receptor which is critical for bone development, we studied, analyzed and compared the activation of FGFR3c over a wide range of fgf1 and fgf2 concentrations. We found that while the strength of fgf2 binding to FGFR3c is lower than the strength of fgf1 binding, the fgf2-bound dimers exhibit higher phosphorylation of the critical tyrosines in the activation loop. As a result, fgf1 and fgf2 elicit a similar FGFR3c response at low, but not at high, concentrations. The results demonstrate the versatility of FGFR3c response to fgf1 and fgf2, and highlight the complexity in fgf signaling.

Keywords: cell signaling, FGFR3, fgf1, fgf2, phosphorylation

There are 18 known fibroblast growth factors, which bind to and activate four related receptor tyrosine kinases, FGFR1 through 4 (13). These growth factors play important roles in embryonic development and in adult life, by controlling cell proliferation, differentiation, and migration. The first two growth factors, fgf1 and fgf2, were identified in the late 1970s, based on their ability to promote proliferation of fibroblasts in vivo (47). They work in paracrine fashion, i.e. influencing the signaling of neighboring cells from a distance, and are secreted from cells in a way that is not completely understood. Both fgf1 and fgf2 do not have cleavable signal peptides and are exported out in a way that is independent of the ER-Golgi machinery (8;9).

Fibroblast growth factors were demonstrated to bind heparin and heparan sulfate (10), which is required for fgf/FGFR-mediated signaling (1113). Now it is well established that 2 FGFRs, 2 fgfs and 1 heparan sulfate molecule form a stable signaling-competent dimer (1416). The two catalytic domains in the dimer are positioned in close proximity and their orientation allows them to cross-phosphorylate each other (1).

The FGF receptors belong to the large receptor tyrosine kinase (RTK) super-family. They consist of extracellular ligand-binding domains with three immunoglobulin (Ig) like motifs (D1-D3), single TM domains and intracellular domains composed of a kinase and regulatory sequences. There are different tissue-specific FGFR isoforms, due to alternative splicing in the intracellular and extracellular domains (2;17;18). The very presence of 18 ligands and 4 receptors, with different isoforms due to alternative splicing, gives the option of diverse response and regulation. Indeed, research thus far has established differences in binding affinities of different fgfs to FGFRs (16;19;20). Fgf1 is known as a universal ligand, capable of activating all FGFRs, while the others, including fgf2, exhibit various degrees of specificity (21;22). In addition to differences in affinities (16;19), differences in structure of ligand-extracellular domain complexes have been reported, and these differences are believed to influence the specificity of the biological response of a particular FGFR isoform (15;23). Yet, it is not yet known how the specific biological response of a receptor is achieved upon stimulation with a particular ligand.

Here we focus on the third member of the FGFR family, FGFR3. FGFR3 has two membrane-bound isoforms, produced via alternative splicing: isoform “b”, expressed in epithelial cells, and isoform “c”, expressed in mesenchymal cells. FGFR3c is critical for the development of the long bones, by mediating pro-differentiation signals in chondrocytes, which are of mesenchymal lineage. Deregulation of FGFR3c signaling leads to disorders of long bone development and gives rise to dwarfism phenotypes of different severity, such as achondroplasia (ACH), thanatophoric dysplasia (TD) and hypochondroplasia (HCH) (2427). Because of its involvement in human growth disorders, FGFR3c signaling, including the response to fgf1 and fgf2, has been a topic of intensive research (21;2838). For instance, it has been shown that BaF3 cells expressing FGFR3c exhibit similar mitogenic activity in response to fgf1 and fgf2 (21). However, a pronounced difference in binding of fgf1 and fgf2 to FGFR3c has been observed (16;20;39). This discrepancy suggests that ligand binding strength is not the only factor that regulates FGFR3c ligand-dependent activation. To gain further insight into this issue, we studied, analyzed and compared the activation of FGFR3 over a wide range of fgf1 and fgf2 concentrations. Our findings demonstrate a different signaling capacity of FGFR3/fgf1 and FGFR3/fgf2 dimers, emphasizing the complexity in fgf signaling.

Materials and methods

Western blots

The wild-type FGFR3 plasmid in the pcDNA 3.1+ vector was a generous gift from D. J. Donoghue at UCSD. Human Embryonic Kidney 293 T cells were transfected with the plasmid using Fugene 6 (Roche) according to the manufacturer’s protocol. Cells were cultured in normal medium for 24 hours following transfection, and then starved in serum-free medium for 24 hours. The effect of ligand was tested by incubating the cells in medium supplemented with fgf1 or fgf2 (Millipore, MA). Proteins from the cell lysates of HEK 293T cells were probed with a FGFR3 (H-100) antibody (sc-9007, Santa Cruz Biotechnology) or phospho-Tyr-FGFR antibody (anti-Y653/654, Cell Signaling Technology), followed by anti-rabbit HRP conjugated antibodies (W4011, Promega).


In cross-linking experiments, dimeric receptors were cross-linked with a membrane impermeable linker (BS3, Pierce). After twenty four hour starvation, cells were incubated with 2mM cross-linker for 30 minutes to 1 hour at room temperature, and then quenched in 20mM Tris-HCl for 15 minutes. After two rinses with ice-cold PBS, the cells were lysed and the receptors were detected using Western blotting. The cross-linked fraction was calculated as SD/S=SD/(SM+SD), where SD is the intensity of the dimeric band and SM is the intensity of the monomeric band.

Titration with fgf1

HEK 293 T cells were cultured in normal medium for 24 hours following transfection, and then starved in serum-free medium for 24 hours. Different concentrations of fgf1 and fgf2 (Millipore, MA), ranging from 5 ng/ml to 5000 ng/ml, were added to the serum-free medium. After incubating for 10 minutes with ligand, cells were lysed as described above, and analyzed using Western blotting.

Quantification of Western Blots

The western blots films were scanned and processed with ImageQuant TL. At least three sets of independent experiment were performed in order to determine the averages and the standard errors. The loading on the gels was adjusted such that all the band intensities were within the so-called linear regime, such that the staining intensities were proportional to the protein concentrations (40).

A physical-chemical description of FGFR3 activation


A simple model of RTK activation, described recently (31), assumes that the activation is a two-step process involving ligand-independent dimerization followed by ligand binding to the unliganded dimer:



Here, M denotes the monomer, while d and D denote the unliganded and liganded FGFR3 dimers, respectively. The ligand in this model is pre-dimerized on heparan sulfates on the cell surface prior to binding to the unliganded dimer (17;41). The two reactions (1) and (2) are coupled: ligand binding depletes the unliganded dimers, and this in turn decreases the monomer concentrations. The reaction constants, K1 and K2, are defined as follows:




The mass balance equations for the total ligand concentration [TL] and the total receptor concentration [TR] are:



As shown in detail in (31), these four equations (3) though (6) can be solved as a function of the reaction constants K1 and K2 and the total ligand and receptor concentrations, [TL] and [TR], yielding [M], [d], and [D]. In particular, the concentration of monomeric receptors [M] is the solution of the following equation (31):


This equation has a single real positive root (31). Once [M] is determined, [d] is calculated using equation (8):


and [D] is calculated using equation (9):


In this model, the concentration of phosphorylated receptors is then calculated according to (31):


where Φd and ΦD are the probabilities for receptor phosphorylation within the unliganded and liganded dimers, respectively.

The model predicts that all receptors are liganded and dimeric at high ligand concentration, such that the phosphorylation reaches saturation above a threshold concentration, remaining constant as more ligand is added. At saturating ligand concentrations, 2[D]sat = [TR] and therefore:


The phosphorylated fraction of receptors [P]/[P]sat is given by (31):


The ratio of Φd and ΦD is:


where [P]0/[P]sat and [d]0/[D]sat are the phosporylated receptor fraction and dimeric receptor fraction in the absence of ligand.

At zero ligand, equation (12) reduces to:


Measurable quantities

The phosphorylation [P] at various ligand concentrations can be measured on Western blots using antibodies that are specific for phosphorylated tyrosines. [P]0 is the phosphorylation measured in the absence of ligand, while [P]sat is the phosphorylation measured at saturating ligand concentration. Thus, the ratio [P]/[P]sat , given by equation (12), can be measured over a wide range of ligand concentrations, including zero ligand ([P]0/[P]sat). The measured [P]/[P]sat can be then fitted to the theoretical prediction given by equation (12), after substituting parameters from equations (8), (9), and (13). The predicted values of [P]/[P]sat depend on the two unknown parameters, K1 and K2, which can be optimized in the fit.

The theoretical prediction of [P]/[P]sat also depends on the value of [d]0/[D]sat, the dimeric fraction at zero ligand (see equation (13)). Since dimeric fraction are difficult to measure directly (31), here we approximate dimeric fractions with the ratio of cross-linked fractions at zero ligand and at saturating ligand concentrations (thus assuming that the efficiency for cross-linking the two receptors in the dimer is not affected by the bound ligand (31)).


The phosphorylation of FGFR3/fgf2 dimers is higher than the phosphorylation of FGFR3/fgf1 dimers

We have previously demonstrated the successful plasma membrane expression of FGFR3c in HEK 293 T cells (42). Here we investigated how FGFR3c responds to the ligands fgf1 and fgf2. After transfection, HEK 293T cells were cultured for 24 hours, starved for 24 hours to induce accumulation of receptors on the plasma membrane (35), and treated with fgf1 or fgf2 (Millipore, MA) at concentrations ranging from 5 ng/ml to 5000 ng/ml. After incubating with the ligands for 10 minutes, the cells were lysed and the lysates were analyzed by Western blot. Total FGFR3 expression was probed by Western blots using anti-FGFR3 antibodies (H-100, Santa Cruz). The phosphorylation of FGFR3 was probed using anti-phospho-FGFR antibodies (anti-Y653/654, Cell Signaling Technology) (31). These antibodies are specific for two phosphorylated tyrosines in the activation loop of FGFR3, Y647 and Y648. The phosphorylation of these two tyrosines is required for the activation of the kinase domain and the phosphorylation of other intracellular tyrosine residues (43).

Representative Western blot experiments are shown in Figure 1. We observe two bands corresponding to the intermediate, 120 kDa Endo H-sensitive FGFR3c found in the ER/cis-Golgi, and the fully glycosylated mature 130 kDa FGFR3, located predominantly on the plasma membrane (31;44). In this work dedicated to ligand binding, we focus at the mature FGFR3c 130 kDa form located predominantly on the cell surface. This is the form that has access to the ligand and thus responds to it. The lower molecular weight FGFR3 located in the ER (120 kDa band) is not exposed to ligands and is not considered in the analysis.

Figure 1
Western blots, showing FGFR3c phosphorylation and expression, as a function of fgf1 concentration (A) and fgf2 concentration (B). In these experiments, HEK 293 T cells were transfected with 1µg of DNA encoding FGFR3c, using Fugene 6 (Roche). 24 ...

The activation model, given by equations (3) through (13) predicts a threshold ligand concentration above which all receptors that are exposed to ligand are in their liganded dimeric state (31). Consistent with this prediction, we see saturation in FGFR3c phosphorylation above the ligand concentration of 1000 ng/ml: phosphor-staining levels stay at their constant maximum values and are not further increased when more ligand is added. As discussed previously, the Western blot protocols that we use ensure that the band intensities are always within the so-called linear range, i.e. the band intensities are proportional to the receptor concentrations (38;40). Under these conditions the saturation we observe is not an experimental artifact, but a true saturation in receptor phosphorylation.

Figure 1 compares the results when cells expressing FGFR3c were treated with different concentrations of fgf1 (top) and fgf2 (bottom). To be able to carry out a comparison of FGFR3c response to different concentrations of the two ligands, the lysates were loaded onto two gels, and the proteins in the two gels were transferred simultaneously onto a blotting membrane and incubated with antibodies. In Figure 1 we see that while the bands at zero ligand are very similar, FGFR3c phosphorylation at saturating fgf2 concentration, [P]satfgf2, is higher than the phosphorylation at saturating fgf1 concentration, [P]satfgf1. Quantification of the 130 kDa mature FGFR3c bands in the plateau region (corresponding to ligand concentration above 1000 ng/ml) yielded the value of [P]satfgf1, obtained by averaging the results above 1250 ng/ml fgf1, as well as the average value of [P]satfgf2. The ratio of [P]satfgf2/[P]satfgf1 was calculated as 2.1. Two additional independent experiments were performed (gels not shown), and the average value for the ratio [P]satfgf2/[P]satfgf1 from the three experiments was determined as 2.1 ± 0.6. To confirm this result, we re-ran lysates for saturating fgf1 and fgf2 concentrations (5000 ng/ml) on one gel (Figure 2). The side-by-side comparison confirms that FGFR3c phosphorylation is higher at saturating fgf2 concentrations than at saturating fgf1 concentrations, and the ratio [P]satfgf2/[P]satfgf1 is 2.0 ± 0.01.

Figure 2
Side-by-side comparison of phosphorylation levels in the absence of ligand and at saturating fgf1 and fgf2 concentrations (5000 ng/ml).

The phosphorylation at saturating ligand concentration is related to the receptor phosphorylation probability within the liganded dimers, ΦD, according to equation (11). The ratio [P]satfgf2/[P]satfgf1 is therefore equal to


Thus, ΦDfgf2/ΦDfgf1=2.0±0.01, i.e., the probability for receptor phosphorylation at Y647/8 within the fgf2-bound FGFR3c dimers is twice as high as the receptor phosphorylation probability at Y647/8 within the fgf1-bound FGFR3c dimers.

To measure how the phosphorylation changes as a function of ligand concentration, we quantified the anti-Y653/4 band intensities for the 130 kDa mature fully glycosylated form, for all studied ligand concentrations, for the representative experiment shown in Figure 1 and for the two additional independent experiments. The average values of the phosphorylated fractions as a function of ligand concentration are shown in Figures 3 and and5.5. In Figure 3, all the data, together with the standard errors, are plotted on a common scale, with the value of [P]satfgf1 set as 1. In Figure 5, the data for fgf1 and fgf2 are replotted on separate scales, after normalizing the two data sets separately such that the plateau level is set to 1 in both cases.

Figure 3
Receptor phoshorylation as a function of fgf1 (solid circles) and fgf2 (open squares) concentration. Data are from three independent Western blot experiments. The phosphorylation is assigned a value of 1 at saturating fgf1 concentrations.
Figure 5
Fits of the RTK activation model, given by equations (3) through (93), to the Western blot data. The two reaction constants, K1 and K2, were varied to achieve the best fit to the data. Fit results are shown in Table 2. (A) Results for wild-type FGFR3 ...

FGFR3c cross-linking propensities are similar at saturating fgf1 and fgf2 concentrations

Next we performed cross-linking experiments at zero ligand and at saturating fgf1 and fgf2 concentrations (Figure 4). Cells were starved for 24 hours and then split into three pools; no ligand was added to the first cell pool, while fgf1 and fgf2 at 2500 ng/ml were added to the second and third pool, respectively. A membrane-impermeable cross-linker was used, such that only the mature 130 kDa FGFR3 on the surface was cross-linked. FGFR3c was detected on Western blots using anti-FGFR3 antibodies (Figure 4). In the absence of ligand, we see a weak band at twice the molecular weight of mature FGFR3c (~260 kDa), corresponding to cross-linked FGFR3. The intensity of the cross-linked band increases in the presence of saturating ligand concentrations. We quantified the bands corresponding to monomeric and cross-linked FGFR3, and determined the fraction of cross-linked FGFR3c as SD/S=SD/(SM+SD), where SD is the intensity of the dimeric band and SM is the intensity of the monomeric 130 kDa band. From four independent experiments, we find that the cross-linked FGFR3c fraction is 0.20±0.03 in the absence of ligand, 0.59 ± 0.08 in the presence of fgf1, and 0.51 ± 0.06 in the presence of fgf2.

Figure 4
Western blots of cross-linked FGFR3c, in the absence of ligand and in the presence of saturating fgf1 and fgf2 concentrations. Cells were incubated with BS3 cross-linking agent (Pierce) for 30 minutes at room temperature prior to lysis. The blot was stained ...

As discussed above, we expect that all 130 kDa receptors are dimeric at saturating ligands concentrations. Yet, only about 55% of the 130kDa form is cross-linked at saturating ligand concentrations. While it is possible that not all receptors are dimeric, it is more likely that the cross-linker has a cross-linking efficiency lower than 100% (as can be expected for the yield of any chemical reaction). For instance, the data at saturating ligand concentration are consistent with ~100% dimer and about 55% cross-linking efficiency.

We see that the cross-linked fractions at saturating fgf1 and fgf2 concentrations are similar (~ 55%). Assuming that cross-linking correlates with dimerization, we conclude that the dimeric fractions are also similar. Thus, the cross-linking data are consistent with the idea that the difference in phosphorylation that we observe in Figure 2 can be attributed to differences in phosphorylation within the liganded dimers, ΦD. Furthermore, we can estimate the dimeric fraction at zero ligand (needed to calculate ΦdD according to equation (11)) as the ratio of cross-linked fractions at zero ligand and at saturating ligand concentration: [d]0/[D]satfgf1=[d]0/[D]satfgf2=0.36±0.1.

fgf1 binding to FGFR3c is stronger than fgf2 binding

The constant K2 in equation (4) is a measure, albeit indirect, of the strength of ligand binding. K2 can be determined by fitting the model given by equations (3) through (13) once the ratios [P]0 /[P]sat, [d]0/[D]sat, and ΦdD are calculated. The value of [d]0/[D]sat was estimated based on cross-linking as described above, and the value of [P]0 /[P]sat is given by the first experimental point in Figure 3. From equation (13), we calculate Φd/ΦDfgf1=0.6±0.2. Because ΦDfgf2/ΦDfgf1=2.0±0.01, we obtain Φd/ΦDfgf2=0.3±0.1.

We determined the receptor concentration [TR] by comparing the expression of the 130kDa FGFR3c in our experiments to FGFR3c expression in the stable HEK293-fWT line, estimated to have 8.4 × 105 copies of mature FGFR3c per cell (31). The transient expression achieved in our experiments was 21 ± 5 % of the stable expression, as determined via a direct expression comparison on a Western blot (results not shown).

Using the above values, the activation model given by equations (3) through (13) was fitted to the experimental data in Figure 5 using a Matlab code, yielding the optimal values of K1 and K2. Initial guesses for K1 and K2 were inputted into the Matlab code, which calculated [d], [D], [M], and the phosphorylated FGFR3 fraction, [P]/[P]sat. This prediction was compared to the experimentally determined phosphorylated fractions and the two unknowns K1 and K2 were varied until the calculated predictions of [P]/[P]sat provided the best description of the experimental data. The optimal parameters, which did not depend on the initial guesses, are shown in Table 1.

Table 1
Optimal values of the apparent dimerization free energy ΔG = -RTlnK1 and the apparent ligand-dissociation constants 1/K2, determined by fitting the theoretical predictions of phosphorylated fractions, given by equations (3) through (13), to the ...

The parameter K2 describes the strength of ligand binding to the “pre-formed” unliganded dimer. The reciprocal value, 1/K2, is a measure of the apparent dissociation constant, in nanomolar units. Table 1 shows that the apparent dissociation constant for fgf2 is an order of magnitude higher than the apparent dissociation constant for fgf1. Thus, the binding strength of fgf1 to FGFR3c is an order of magnitude higher than the strength of fgf2-FGFR3c binding.

The optimal value of the dimerization constant K1, obtained in the fit, was used to calculate the apparent free energy of ligand-independent dimerization, ΔG = −RT ln K1. Two K1 values were obtained in the two independent fits of the fgf1 and fgf2 data, and the respective free energies are shown in Table 1. The results pertain to the very same process, dimerization in the absence of ligand, and are thus expected to be the same. As seen in Table 1, the calculated dimerization free energies are indeed very similar, demonstrating the consistency of the data analysis.


Binding of fgf1 and fgf2 to subdomains D2 and D3 of FGFR3c extracellular domains has been measured previously in vitro using surface plasmon resonance (20). These measurements revealed a difference in ligand binding strength: fgf1 binding was stronger than fgf2 binding. Such difference in binding was also observed using a soluble chimeric protein, consisting of FGFR3c extracellular domain fused to human placental alkaline phosphatase (39). To investigate if such a difference is also observed for full-length FGFR3c, we measured FGFR3c phosphorylation over a wide ligand concentration range, and we fitted the model described by equations (3) through (13) to these phosphorylation measurements. In our experiments we used a very broad range of ligand concentrations, including very high ligand concentrations, in order to determine the relevant parameters describing ligand binding. While the high ligand concentrations that we used here are not biologically relevant, this approach allowed us to compare not only the binding strength of the two ligands to FGFR3c, but also compare the relative ease of Y647/648 phosphorylation within the fgf1 and fgf2-bound FGFR3c dimers. The conclusions that we drew based on these experiments pertain to the physical-chemical and biochemical characteristics of FGFR3c, and thus should be always valid.

The experimental approach that we used here to monitor ligand binding was indirect, and the apparent ligand binding constant K2 determined in the fit is not a direct measure of ligand binding affinity. Yet, this approach allowed us to monitor FGFR3c-ligand interactions that are productive (i.e resulting in activation) such that the binding strengths of fgf1 and fgf2 can be compared in a biological context. We found that the apparent fgf1-FGFR3c dissociation constant is an order of magnitude lower than the fgf2-FGFR3c dissociation constant (see Table 1). Thus, our results are consistent with previous studies of the isolated extracellular domain (20;39), confirming stronger binding for fgf1 to FGFR3c, as compared to fgf2.

At high ligand concentrations, we observe a plateau in FGFR3c phosphorylation (Figures 3 and and5),5), similar to previous studies (31). In the plateau, FGFR3c exists predominantly as liganded dimers (31), and thus the difference at high ligand concentrations can be attributed to the receptor phosphorylation levels in the liganded dimeric state. Our results showed that the receptor phosphorylation in the fgf2-bound dimers is exactly twice as high as the receptor phosphorylation in the fgf1-bound dimers (Figure 2). This intriguing finding demonstrates a different signaling capacity of FGFR3/fgf1 and FGFR3/fgf2 dimers.

RTK activation is known to require precise orientation and positioning of the catalytic domains with respect to each other, such that the phosphate group can be successfully transferred from ATP to the neighboring receptor. For EGFR, this entails the formation of an asymmetric kinase dimer in which the C-lobe of one kinase contacts the N-lobe of the second kinase and positions the activation loop of the second kinase to catalyze phosphate group transfer (45). Similar asymmetric interactions have been proposed for FGF receptors (46). A question remains, however, whether the two kinases alternate over time and act both as catalyst and substrate. Our finding that receptor phosphorylation levels in the fgf2-bound FGFR3c dimers are exactly twice as high as receptor phosphorylation levels in the fgf1-bound dimers may suggest that only one of the receptors is phosphorylated at Y647/8 in the fgf1-bound dimer, while both of them are phosphorylated in the fgf2-bound dimer. This implies that either the kinase structures and/or orientations in the fgf1-FGFR3c and fgf2-FGFR3c dimers are different, or the conformational flexibilities are different in the two structures.

Perhaps the most interesting finding in this work is that the differences in ligand binding strength and in receptor phosphorylation levels in the liganded dimeric state compensate each other, such that the measured phosphorylation is similar at ligand concentrations below 500 ng/ml (or 30 nM) (Figure 3). The measured phosphorylation, however, is very different at higher ligand concentration. Thus, the ligand concentration range determines if the response to fgf1 and fgf2 is different or the same. This is a demonstration of the versatility of FGFR3c response to fgf1 and fgf2. Note that FGFR3c plays a critical role during development, when one can expect large fluctuations in ligand expression. We can therefore expect that the differences in FGFR3c response to ligand that we report here are important for human development.

The finding that the measured FGFR3c phosphorylation is similar in response to low fgf1 and fgf2 concentrations (Figure 3) is consistent with previous results that the FGFR3c-mediated mitogenic activities of fgf1 and fgf2 are the same (21). At first glance, these results seem to contradict the reports for different ligand binding strengths (20;39). The presented work resolves the controversy, provides new knowledge about FGFR3c activation, and highlights the complexity in fgf signaling.


Supported by NIH GM068619.

We thank Dr. Lijuan He was many useful discussions.


fibroblast growth factor 3
fibroblast growth factor
HEK 293 T
human embryonic kidney 293 T

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