PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of narLink to Publisher's site
 
Nucleic Acids Res. 1986 March 25; 14(6): 2413–2427.
PMCID: PMC339673

Cloning and expression of cDNA encoding mouse tyrosinase.

Abstract

We have isolated a pigment cell-specific cDNA clone from a B16 mouse melanoma cDNA library by differential hybridization. The mRNA of isolated cDNA is highly expressed in B16 melanoma cells and in black mouse (C57BL/6) skin, but is not detectable in mouse neuroblastoma cells nor in K1735 mouse amelanotic melanoma cells. The protein sequence deduced from the nucleotide sequence of the cloned cDNA shows significant similarity to the entire region of Neurospora tyrosinase. To know the identity of cDNA, we transfected K1735 amelanotic melanoma and COS-7 cells with the cDNA carried in a simian virus 40 vector (pKCRH2). We confirmed that the isolated cDNA encodes mouse tyrosinase by immunofluorescence staining of transfected cells using two different anti-T4-tyrosinase monoclonal antibodies. Tyrosinase is composed of 513 amino acids with a molecular weight of 57,872 excluding a hydrophobic signal peptide of 24 amino acids.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • LERNER AB, FITZPATRICK TB, CALKINS E, SUMMERSON WH. Mammalian tyrosinase; the relationship of copper to enzymatic activity. J Biol Chem. 1950 Dec;187(2):793–802. [PubMed]
  • Nishioka K. Particulate tyrosinase of human malignant melanoma. Solubilization, purification following trypsin treatment, and characterization. Eur J Biochem. 1978 Apr;85(1):137–146. [PubMed]
  • LERNER AB, FITZPATRICK TB. Biochemistry of melanin formation. Physiol Rev. 1950 Jan;30(1):91–126. [PubMed]
  • Körner AM, Pawelek J. Dopachrome conversion: a possible control point in melanin biosynthesis. J Invest Dermatol. 1980 Aug;75(2):192–195. [PubMed]
  • Pawelek J, Körner A, Bergstrom A, Bologna J. New regulators of melanin biosynthesis and the autodestruction of melanoma cells. Nature. 1980 Aug 7;286(5773):617–619. [PubMed]
  • Körner A, Pawelek J. Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin. Science. 1982 Sep 17;217(4565):1163–1165. [PubMed]
  • HOCHSTEIN P, COHEN G. The cytotoxicity of melanin precursors. Ann N Y Acad Sci. 1963 Feb 15;100:876–886. [PubMed]
  • Pawelek JM. Factors regulating growth and pigmentation of melanoma cells. J Invest Dermatol. 1976 Apr;66(4):201–209. [PubMed]
  • Pawelek JM, Lerner AB. 5,6-Dihydroxyindole is a melanin precursor showing potent cytotoxicity. Nature. 1978 Dec 7;276(5688):626–628. [PubMed]
  • Wick MM. An experimental approach to the chemotherapy of melanoma. J Invest Dermatol. 1980 Feb;74(2):63–65. [PubMed]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed]
  • Aviv H, Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. [PubMed]
  • Okayama H, Berg P. High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982 Feb;2(2):161–170. [PMC free article] [PubMed]
  • McMaster GK, Carmichael GG. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. [PubMed]
  • Mishina M, Kurosaki T, Tobimatsu T, Morimoto Y, Noda M, Yamamoto T, Terao M, Lindstrom J, Takahashi T, Kuno M, et al. Expression of functional acetylcholine receptor from cloned cDNAs. Nature. 1984 Feb 16;307(5952):604–608. [PubMed]
  • Kripke ML. Speculations on the role of ultraviolet radiation in the development of malignant melanoma. J Natl Cancer Inst. 1979 Sep;63(3):541–548. [PubMed]
  • Fidler IJ, Gruys E, Cifone MA, Barnes Z, Bucana C. Demonstration of multiple phenotypic diversity in a murine melanoma of recent origin. J Natl Cancer Inst. 1981 Oct;67(4):947–956. [PubMed]
  • Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell. 1981 Jan;23(1):175–182. [PubMed]
  • Graham FL, van der Eb AJ. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. [PubMed]
  • Wigler M, Pellicer A, Silverstein S, Axel R. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell. 1978 Jul;14(3):725–731. [PubMed]
  • Tomita Y, Montague PM, Hearing VJ. Anti-T4-tyrosinase monoclonal antibodies--specific markers for pigmented melanocytes. J Invest Dermatol. 1985 Nov;85(5):426–430. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Toh H, Hayashida H, Miyata T. Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus. Nature. 305(5937):827–829. [PubMed]
  • Burnett JB. The tyrosinases of mouse melanoma. Isolation and molecular properties. J Biol Chem. 1971 May 25;246(10):3079–3091. [PubMed]
  • Hearing VJ, Ekel TM, Montague PM. Mammalian tyrosinase: isozymic forms of the enzyme. Int J Biochem. 1981;13(1):99–103. [PubMed]
  • Tomita Y, Hariu A, Kato C, Seiji M. Transfer of tyrosinase to melanosomes in Harding-Passey mouse melanoma. Arch Biochem Biophys. 1983 Aug;225(1):75–85. [PubMed]
  • SEIJI M, SHIMAO K, BIRBECK MS, FITZPATRICK TB. Subcellular localization of melanin biosynthesis. Ann N Y Acad Sci. 1963 Feb 15;100:497–533. [PubMed]
  • Gorman CM, Moffat LF, Howard BH. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. [PMC free article] [PubMed]
  • Kozak M. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984 Jan 25;12(2):857–872. [PMC free article] [PubMed]
  • Goeddel DV, Leung DW, Dull TJ, Gross M, Lawn RM, McCandliss R, Seeburg PH, Ullrich A, Yelverton E, Gray PW. The structure of eight distinct cloned human leukocyte interferon cDNAs. Nature. 1981 Mar 5;290(5801):20–26. [PubMed]
  • Proudfoot NJ, Brownlee GG. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. [PubMed]
  • Blobel G, Dobberstein B. Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components. J Cell Biol. 1975 Dec;67(3):852–862. [PMC free article] [PubMed]
  • Steiner DF, Quinn PS, Chan SJ, Marsh J, Tager HS. Processing mechanisms in the biosynthesis of proteins. Ann N Y Acad Sci. 1980;343:1–16. [PubMed]
  • Miyazaki K, Otaki N. Tyrosinase as glycoprotein. Arch Dermatol Forsch. 1975;252(3):211–216. [PubMed]
  • Herrmann WP, Uhlenbruck G. Serological studies on the carbohydrate moiety of human tyrosinase. Arch Dermatol Res. 1975 Dec 31;254(3):275–280. [PubMed]
  • Ohkura T, Yamashita K, Mishima Y, Kobata A. Purification of hamster melanoma tyrosinases and structural studies of their asparagine-linked sugar chains. Arch Biochem Biophys. 1984 Nov 15;235(1):63–77. [PubMed]
  • Marshall RD. The nature and metabolism of the carbohydrate-peptide linkages of glycoproteins. Biochem Soc Symp. 1974;(40):17–26. [PubMed]
  • Bause E. Structural requirements of N-glycosylation of proteins. Studies with proline peptides as conformational probes. Biochem J. 1983 Feb 1;209(2):331–336. [PubMed]
  • Lerch K. Primary structure of tyrosinase from Neurospora crassa. II. Complete amino acid sequence and chemical structure of a tripeptide containing an unusual thioether. J Biol Chem. 1982 Jun 10;257(11):6414–6419. [PubMed]
  • Lerch K. Neurospora tyrosinase: molecular weight, copper content and spectral properties. FEBS Lett. 1976 Oct 15;69(1):157–160. [PubMed]
  • Dwulet FE, Putnam FW. Internal duplication and evolution of human ceruloplasmin. Proc Natl Acad Sci U S A. 1981 May;78(5):2805–2809. [PubMed]
  • Pfiffner E, Lerch K. Histidine at the active site of Neurospora tyrosinase. Biochemistry. 1981 Oct 13;20(21):6029–6035. [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press