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Logo of biolprocBioMed CentralBiomed Central Web Sitesearchsubmit a manuscriptregisterthis articleBiological Procedures OnlineJournal Front Page
 
Biol Proced Online. 2012; 14: 4.
Published online 2012 April 2. doi:  10.1186/1480-9222-14-4
PMCID: PMC3388579

A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate

Abstract

Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describe a technique that couples the simultaneous quantification of radio-labeled calcium accumulation in endoplasmic reticulum (ER) microsomes with the release of inorganic phosphate (Pi) by the hydrolytic activity of sarco-endoplasmic reticulum calcium ATPase (SERCA) all in the convenience of a 96-well format.

Keywords: Calcium, SERCA activity, Microsomes, Inorganic phosphate, Malachite green

Articles from Biological Procedures Online are provided here courtesy of BioMed Central