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Biotechnol Res Int. 2012; 2012: 472307.
Published online Jun 20, 2012. doi:  10.1155/2012/472307
PMCID: PMC3388318
Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep
Elena Dehnavi, 1 * Mojtaba Ahani Azari, 1 Saeed Hasani, 1 Mohammad Reza Nassiry, 2 Mokhtar Mohajer, 3 Alireza Khan Ahmadi, 4 Leila Shahmohamadi, 1 and Soheil Yousefi 1
1Department of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, P.O. Box 4913815739, Gorgan, Iran
2Department of Animal Science, Ferdowsi University of Mashhad, P.O. Box 9177948978, Mashhad, Iran
3Golestan Agriculture Jahad, P.O. Box 49174, Gorgan, Iran
4Department of Animal Science, Faculty of Agrecultural Scinece and Natural Recources, Gonbad University, P.O. Box 4971799151, Gonbad, Iran
*Elena Dehnavi: e.dehnavi/at/yahoo.com
Academic Editor: Manuel Canovas
Received March 27, 2012; Accepted April 23, 2012.
Abstract
The aim of present study was to investigate myostatin gene polymorphism and its association with yearling weight records in Zel sheep using PCR-RFLP and PCR-SSCP methods. Blood samples were collected from 200 Zel sheep, randomly, and DNA was extracted using modified salting out method. Polymerase chain reaction was carried out to amplify 337, 222, and 311 bp fragments, respectively, comprising a part of exon 3, intron 1, and intron 2 of myostatin gene. In addition, exon 3 was digested by HaeIII enzyme under RFLP method, and introns 1 and 2 were studied using SSCP. Under RFLP method, all samples showed mm genotype. Under SSCP method, intron 1 was also monomorph but intron 2 was polymorph (AA, AB, and BB). The allelic frequencies for A and B were 75.5 and 24.5%, respectively. This locus was not in Hardy-Weinberg equilibrium (P < 0.05), and there was no significant effect of myostatin gene on yearling weights.
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