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Nucleic Acids Res. 1988 October 25; 16(20): 9477–9487.
PMCID: PMC338757

A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga.

Abstract

A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.

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Selected References

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  • Van Etten JL, Burbank DE, Schuster AM, Meints RH. Lytic viruses infecting a Chlorella-like alga. Virology. 1985 Jan 15;140(1):135–143. [PubMed]
  • Schuster AM, Burbank DE, Meister B, Skrdla MP, Meints RH, Hattman S, Swinton D, Van Etten JL. Characterization of viruses infecting a eukaryotic Chlorella-like green alga. Virology. 1986 Apr 15;150(1):170–177. [PubMed]
  • Van Etten JL, Schuster AM, Girton L, Burbank DE, Swinton D, Hattman S. DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. Nucleic Acids Res. 1985 May 24;13(10):3471–3478. [PMC free article] [PubMed]
  • Xia YN, Burbank DE, Uher L, Rabussay D, Van Etten JL. Restriction endonuclease activity induced by PBCV-1 virus infection of a Chlorella-like green alga. Mol Cell Biol. 1986 May;6(5):1430–1439. [PMC free article] [PubMed]
  • Xia Y, Burbank DE, Van Etten JL. Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga. Nucleic Acids Res. 1986 Aug 11;14(15):6017–6030. [PMC free article] [PubMed]
  • Xia YN, Narva KE, Van Etten JL. The cleavage site of the RsaI isoschizomer, CviII, is G decreases TAC. Nucleic Acids Res. 1987 Dec 10;15(23):10063–10063. [PMC free article] [PubMed]
  • Xia YN, Burbank DE, Uher L, Rabussay D, Van Etten JL. IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity. Nucleic Acids Res. 1987 Aug 11;15(15):6075–6090. [PMC free article] [PubMed]
  • Marinus MG, Carraway M, Frey AZ, Brown L, Arraj JA. Insertion mutations in the dam gene of Escherichia coli K-12. Mol Gen Genet. 1983;192(1-2):288–289. [PubMed]
  • Schleif R. Assaying of organisms for the presence of restriction endonucleases. Methods Enzymol. 1980;65(1):19–23. [PubMed]
  • Peacock AC, Dingman CW. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry. 1967 Jun;6(6):1818–1827. [PubMed]
  • Jeppesen PG. Separation and isolation of DNA fragments using linear polyacrylamide gradient gel electrophoresis. Methods Enzymol. 1980;65(1):305–319. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • McGraw RA., 3rd Dideoxy DNA sequencing with end-labeled oligonucleotide primers. Anal Biochem. 1984 Dec;143(2):298–303. [PubMed]
  • Kuo TT, Huang TC, Teng MH. 5-Methylcytosine replacing cytosine in the deoxyribonucleic acid of a bacteriophage for Xanthomonas oryzae. J Mol Biol. 1968 Jul 14;34(2):373–375. [PubMed]
  • Blakesley RW, Dodgson JB, Nes IF, Wells RD. Duplex regions in "single-stranded" phiX174 DNA are cleaved by a restriction endonuclease from Haemophilus aegyptius. J Biol Chem. 1977 Oct 25;252(20):7300–7306. [PubMed]
  • Rogers SG, Rhoades M. Bacteriophage T5-induced endonucleases that introduce site-specific single-chain interruptions in duplex DNA. Proc Natl Acad Sci U S A. 1976 May;73(5):1576–1580. [PubMed]
  • Burton WG, Roberts RJ, Myers PA, Sager R. A site-specific single-strand endonuclease from the eukaryote Chlamydomonas. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2687–2691. [PubMed]

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