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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Science. Author manuscript; available in PMC 2012 September 9.
Published in final edited form as:
PMCID: PMC3385415
NIHMSID: NIHMS384901

Unique Processing During a Period of High Excitation/Inhibition Balance in Adult-Born Neurons

Abstract

The adult dentate gyrus generates new granule cells (GCs) that develop over several weeks and integrate into the preexisting network. While adult hippocampal neurogenesis has been implicated in learning and memory, the specific role of new GCs remains unclear. We examined whether immature adult-born neurons contribute to information encoding. Combining calcium imaging and electrophysiology in acute slices we found that weak afferent activity recruits few mature GCs while activating a substantial proportion of the immature neurons. These different activation thresholds are dictated by an enhanced excitation/inhibition balance transiently expressed in immature GCs. Immature GCs exhibit low input specificity that switches with time towards a highly specific responsiveness. Therefore, activity patterns entering the dentate gyrus can undergo differential decoding by a heterogeneous population of GCs originated at different times.

The adult hippocampus continuously generates new neurons that integrate in the dentate gyrus network and become relevant for information processing during specific learning tasks (17). Experimental manipulations that reduce adult neurogenesis produce impairment of hippocampus-dependent learning and behavior (8, 9). Yet the specific traits that determine the functional relevance of adult-born neurons remain unknown (10, 11). Is it solely the continuous addition of new neurons to the network what is important, or are there unique functional properties only attributable to new GCs that are relevant to information processing?

When reaching maturity, adult-born GCs exhibit functional properties that are indistinguishable from GCs generated during development (3). However, while developing, immature GCs display elevated intrinsic excitability, reduced GABAergic inhibition and a capacity to undergo activity-dependent synaptic potentiation (1216). Such high intrinsic excitability would potentially allow immature GCs to be activated by entorhinal afferents in spite of their low density of glutamatergic inputs (17). It has thus recently been hypothesized that immature GCs might be critical to hippocampal function (1820).

First, we investigated how immature GCs process afferent activity from entorhinal inputs and how they compare to mature GCs in the adult mouse hippocampus. We selected four-week-old neurons because this is the earliest stage at which adult-born GCs can be reliably activated by an excitatory drive (17). Adult-born GCs were retrovirally labeled to express RFP and acute hippocampal slices were prepared four weeks post retroviral injection (4 wpi). Time-lapse calcium imaging was performed using Oregon Green BAPTA-1 AM (OGB-1 AM) to monitor the activation of immature (4 wpi, RFP+) and mature (RFP) GCs in response to medial perforant path (mPP) stimulation (Fig. 1A–D; see S.O.M. and Fig. S1). Ensemble maps representing active neuronal populations were obtained at increasing input strengths (Fig. 1E, S2A–C). The number of active immature and mature GCs increased with stronger stimuli. Each stimulus consistently activated a larger proportion of the immature GC population (Fig. 1F), suggesting that immature neurons require weaker inputs to trigger a spike.

Fig. 1
Enhanced activation of immature GCs

We used electrophysiological recordings to dissect the mechanisms involved in the differential activation of immature and mature GCs. We characterized the activation profile of GCs at the single cell level using loose patch recordings to detect spikes in response to mPP activation (Fig. 2A–C). Stimuli of increasing intensity elicited spikes with increasing probability in all GCs, yet mature GCs demanded stronger inputs in order to spike. We measured the input strength required to elicit 50 % spiking probability (hereafter called threshold input, Fig. 2C, see S.O.M.). A cumulative distribution of the threshold inputs was then used to build the activation curves, which represent the fractional recruitment of the GC populations as a function of the input strength (Fig. 2D). The activation curve corresponding to mature GCs is shifted toward higher input strengths with respect to immature neurons. As an example, a stimulus that recruits ~5 % of mature neurons activates ~30 % of immature GCs.

Fig. 2
Differential influence of inhibitory circuits in the activation of immature and mature GCs

These data indicate that the dentate gyrus comprises a heterogeneous population of GCs in which different subpopulations display diverse activation thresholds, and support the observations obtained using immediate early gene expression that adult-born neurons could participate in information processing in hippocampus-dependent tasks (5, 21, 22). To investigate how immature and mature GCs respond to conditions of activity that resemble those occurring during hippocampus-dependent behaviors (23), we measured spiking in response to 10 Hz trains delivered to the mPP. We found that immature GCs fire repeatedly during the train whereas mature GCs fire at most a single spike (Fig. 2F,G). Our results suggest that immature GCs might be activated during behavior by entorhinal inputs with higher probability than mature GCs.

The high intrinsic excitability of immature GCs is sufficient to compensate for their weak glutamatergic inputs but it does not predict their lower threshold input for activation (17). However, mPP axons not only produce monosynaptic glutamatergic excitation of GCs but also they recruit in a feedforward manner GABAergic inhibitory circuits, which can modulate neuronal firing in response to afferent inputs (2426). We therefore investigated the role of inhibition in the activation of GCs. Blocking GABAergic inhibition with picrotoxin (PTX) induced a significant reduction in the input strength required to activate mature but not immature GCs (Fig. 2C–E).

The developmental GABA switch from depolarizing to hyperpolarizing occurs in adult-born neurons (27). The inhibitory nature of GABAergic inputs in 4 wpi GCs was corroborated by means of perforated patch recordings and rendered hyperpolarized values of GABA reversal potential for all GCs (Fig. S3). We then investigated the precise contribution of excitatory and inhibitory components that control the activation of GCs. We activated mPP axons and measured the threshold input of GCs in loose patch recordings. Subsequently, we performed whole-cell recordings in the same neurons to measure excitatory and inhibitory responses elicited at threshold input (Fig. 3A, see also Fig. S4). Activation of mPP produced excitatory postsynaptic currents (EPSCs) and inhibitory postsynaptic currents (IPSCs), indicating that glutamatergic entorhinal axons directly activate immature and mature GCs and also recruit inhibitory interneurons that synapse into both populations. Yet, the maximal conductance of both excitatory and inhibitory responses (EPSG and IPSG) was substantially larger in mature GCs (Fig. 3A–C), maintaining a similar ratio of peak excitation/inhibition and reflecting the higher density of glutamatergic and GABAergic contacts characteristic of fully developed neurons (3, 14). Notably, 4 wpi neurons displayed a significant delay in the onset of inhibition, which occurred at a time that followed spiking in those cells (Fig. 3B,D). Thus, it is very unlikely that spike initiation in immature GCs is controlled by inhibitory circuits. We then hypothesized that the observed difference in the activation threshold relied on the excitation/inhibition balance at the precise moment of spiking. Indeed, excitation onto immature GCs (~4 nS) was about 4-fold that of inhibition at the moment of spiking (~1 nS), whereas that ratio was 2-fold in mature GCs (~6 and ~3 nS, Fig. 3E). To determine whether this difference was due to the slow maturation of perisomatic GABAergic synapses characteristic of newly generated GCs (14, 28), we compared the strength and kinetics of direct inhibition onto 4 wpi and mature GCs. We found that direct perisomatic inhibition was slower in immature than mature GCs, while no differences were found for dendritic IPSCs (Fig. S5). These findings indicate that the slow disynaptic inhibition kinetics observed after mPP stimulation is due to a slow IPSC rather than to a delayed recruitment of inhibitory neurons.

Fig. 3
A differential excitation/inhibition balance underlying the increased activation of immature GCs

The observation that few active mPP terminals are sufficient to recruit immature GCs suggests that this neuronal population could respond to most inputs, acting as good integrators of afferent information. On the other hand, mature GCs that display a higher activation threshold may be recruited by specific inputs, acting as better separators (9, 20). We obtained a quantitative assessment of these properties using calcium imaging to detect activation of neuronal ensembles in response to stimulation of two independent mPP inputs (Fig. 4A–G, Fig. S6). Inputs 1 and 2 were activated separately and recruited distinct neuronal ensembles containing both immature and mature GCs, with some cells shared by both inputs. We define cells activated independently by both inputs as good integrators. We quantified the input integration capacity as the number of GCs recruited by both inputs, normalized to the total number of cells recruited by inputs 1 and 2. The ability of a neuronal ensemble to integrate inputs increased with stimulus strength, as additional mature and immature GCs were recruited by both inputs (Fig. 4H). Strikingly, immature GCs exhibited higher levels of integration along the entire input range. Thus, input strengths of ~10 % resulted in ~20% integration in mature neurons and ~40 % integration in 4 wpi GCs. Such enhanced integration capacity was selective for immature neurons, because RFP+ neurons of 8 weeks of age (8 wpi, when functional properties are fully developed (3)) displayed similar input integration to RFP GCs. Finally, the role of inhibition was assessed by PTX blockade of GABA-mediated signaling. PTX increased the ability of mature neurons to integrate independent inputs without altering the response of immature neuronal ensembles (Fig. 4I,J), in agreement with our observation that inhibition reduces the activation of mature but not immature GCs (Fig. 2D,E).

Fig. 4
Enhanced integration of inputs by immature GCs

Our data demonstrate that immature GCs exhibit all of the features required to process information and, notably, display a low activation threshold due to an enhanced excitation/inhibition balance at the time of spike initiation. At this early developmental stage, they already release glutamate onto CA3 pyramidal cells (Fig. S7). As a consequence, neuronal activity in the dentate gyrus is biased towards the immature population of principal neurons that bypass inhibitory control, while the activation of mature neurons is limited by inhibition (Fig. 1, ,22 and S8). This is in contrast to other areas of the hippocampus and neocortex, where inhibition exerts a global (homogeneous) control in the activity of principal cells (25, 29, 30). Hence, adult neurogenesis emerges as a mechanism that generates a unique type of network heterogeneity. In addition, the differential control of inhibition revealed here constitutes a simple synaptic mechanism that could underlie the enhanced capacity of immature GCs to undergo activity-dependent synaptic potentiation when GABAergic inhibition is left intact (12, 13, 31). In the context of the low activation threshold described here, the increased plasticity might provide an efficient means for strengthening and reinforcing weak synaptic inputs that are repeatedly activated during a restricted time window.

The observed network heterogeneity may be crucial for the integration and separation of spatial patterns, properties that have been attributed to the dentate gyrus (10). Their low activation threshold and low input specificity make immature GCs appropriate substrates for pattern integration, a feature that has been proposed in computational models of adult neurogenesis (10, 18, 19, 32). In this context, immature neurons represent a population of integrators that are broadly tuned during a transient period and may encode most features of the incoming afferent information. When becoming mature, new GCs display high activation threshold and input specificity and will, therefore, become good pattern separators. Adult neurogenesis would then maintain the renewable cohorts of highly integrative GCs in the dentate gyrus. Finally, the unique functional properties described here support a hypothesis whereby activity reaching the dentate gyrus could undergo differential decoding through immature neuronal cohorts that are highly responsive and integrative and, in parallel, through a large population of mature GCs with sparse activity and high input specificity.

Supplementary Material

Supplementary data 1

ACKNOWLEDGMENTS

We thank Mariela Veggetti for technical assistance, and Brad Aimone, Yan Li, Fred Gage and Massimo Scanziani for insightful discussions. A.M.B., L.A.M. and A.F.S. are investigators of the National Research Council (CONICET). M.B.P. was supported by a CONICET fellowship. This work was supported by the Guggenheim Fellowship and by grants from the National Institutes of Health (FIRCA R03TW008607-01), the Howard Hughes Medical Institute (grant #55005963) and the ANPCyT PICT2008 to A.F.S. and PICT2010 to A.M.B. and A.F.S.

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