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There are strong and direct relationships between undernutrition and the disease caused by infectious organisms, including the diverse pathogens labeled as neglected tropical diseases (NTDs). Undernutrition increases the risk of infection, the severity of disease and the risk that children will die, while the physical damage, loss of appetite, and host responses during chronic infection can contribute substantially to undernutrition. These relationships are often synergistic. This opinion article examines the role of nutrition in controlling NTDs and makes the point that mass drug treatment - the major strategy currently proposed to control several diseases - is crucial to controlling disease and transmission, but is only the start of the process of physical recovery. Without adequate energy and nutrients to repair damaged tissues or recover lost growth and development, the benefits of treatment may not be evident quickly; the effects of control programs may be not appreciated by beneficiaries; while vulnerability to reinfection and disease may not be reduced. There is substantial potential for nutritional interventions to be added to large-scale programs to deliver drug treatments and thereby contribute, within a broad strategy of public health interventions and behavior change activities, to controlling and preventing NTDs in populations, and to restoring their health.
The fundamental basis of the relationship between an infectious organism and its host is nutritional because the host is the source of all nutrients needed by the organism for maintenance, growth, and reproduction . But the impact of obligate parasites on the nutritional status of a host is not due just to a requirement for nutrients, it is due mainly to the host's responses to infection which lead to an increased metabolic rate, loss of appetite, immune responses, and pathological changes in tissues . Although some of these reactions may be protective to a degree during acute infection, during repeated or chronic infection they can cause the host to become undernourished, especially if the diet is already poor. Undernutrition also increases susceptibility to infection and increases the severity of disease which can lead to a downward spiral of increasing undernutrition and repeated or persistent disease. In this opinion article we argue that breaking out of that spiral requires not just treating disease but treating undernutrition as well, and that nutrition needs to become an important component of integrated programs to control neglected tropical diseases (NTDs).
The relatively new classification by the World Health Organization (WHO) of 17 diseases as 'NTDs' includes eight that are caused by at least 23 species of parasitic worms or helminths . The five NTDs of major interest for control efforts listed in Table Table11 (intestinal worms, lymphatic filariasis, onchocerciasis, schistosomiasis, and trachoma) have been identified because of the large numbers of people infected, but mainly because there are safe, inexpensive, single-dose drugs to treat them by mass chemotherapy . The hope is to make drugs available free or at a very low cost and deliver them in 'community directed treatment' using a model developed during onchocerciasis control programs  or by school teachers to their pupils [6,7]. Mass treatment serves also to decrease transmission by killing larvae or female worms in human hosts, the source of infectious stages. The WHO call this second aim 'preventive chemotherapy'  but it does not prevent reinfection with helminths that have infectious stages either already in the environment or developing within intermediate hosts (see Table Table1),1), so repeated treatment is required as well as public health measures and changes in human behavior . This means that chemotherapy alone is not enough to prevent reinfection and does not treat existing undernutrition either, for reasons that will be explained.
The NTDs listed in Table Table11 are widely distributed across the tropics but the major burden of disease occurs in the mainland countries of sub-Saharan Africa where there is transmission of between two and six diseases . These diseases tend to occur amongst the poorest of the poor who are already at risk of diarrheal diseases, respiratory tract infections, malaria, and HIV. But what is sometimes forgotten is that these diseases also occur among people who often suffer from chronic undernutrition as well. For example, Figure Figure11 shows that there are statistically significant associations between the number of NTDs in 47 countries in Africa and the average prevalence of anemia both in children aged < 5 years and in pregnant women, and with the average prevalence of underweight in young children.
Although parasites are dependent on their hosts for their nutritional needs, nutrition is only the first of four inter-related factors that influence the outcome of infection. The second is the virulence of the pathogen, which is often related to the multiplication of microparasites such as bacteria and protozoa, or to the numbers of macroparasites such as helminths acquired, and to where and how they live within the host and cause disease. The third factor is the innate susceptibility of the host to infection, which may be related to the presence of specific receptors on body surfaces or to non-specific defenses against infection such as gastric acidity. The fourth factor is acquired resistance to infection, mediated by cellular and humoral immune responses, both of which are influenced by nutritional status .
Although both virulence and susceptibility are largely genetically mediated, the occurrence or outcome of disease can be influenced by the nutritional status of the host. This is illustrated in Figure Figure22 based on a theoretical model developed by Scrimshaw and colleagues , who proposed that if the organism is extremely virulent or avirulent, then nutrition will probably have no effect. But the main influence of nutrition, indicated by the probability cloud in Figure Figure2,2, is in the central ground of moderate virulence and moderate susceptibility where most pathogens and hosts probably lie as a result of natural selection and regression to the mean. The probability that nutrition has an effect at the extremes of virulence and susceptibility is low; the probability is highest when both are moderate.
Parasitic organisms and nutrition have separate and combined effects on their host: nutritional status can affect the disease caused by parasitic organisms, parasitic organisms can impair nutritional status, and the two may interact so that their combined effects are synergistic or antagonistic.
A first point to make is that for many NTDs there is a difference between being infected and being diseased. The number of organisms greatly affects the severity of disease and any nutritional effects, particularly for helminths, although the ocular bacterial load of Chlamydia trachomatis has also been shown to be related to the severity of trachoma , so the relationship is not confined to macroparasites. For this reason the aim of programs to control intestinal helminths and schistosomiasis is usually to reduce worm loads so that the risk of disease is minimal , although there is a global goal to eliminate onchocerciasis and lymphatic filariasis.
The evidence for the effect of nutrition on infection and vice versa, is hard to obtain. A few longitudinal studies of children have clearly shown that repeated episodes of respiratory and intestinal infections, including worms, are associated with undernutrition and growth failure [15,16]. But as such prospective studies are uncommon and are ethically questionable, much of the evidence comes from cross-sectional surveys. These are difficult to interpret because they suffer from reverse causality bias: did infection cause undernutrition or did undernutrition increase the risk of infection, as undernutrition is both an outcome and a risk factor. For example, if a wasted child is heavily infected with worms did the worms cause the undernutrition or did the undernutrition predispose the child to heavy infection? For these reasons experimental studies of malnourished cells or animals provide the principal means to examine the relationship between nutrition and infection.
To help understand how undernutrition may influence the processes of infection and disease, Figure Figure33 shows a diagrammatic flow chart between five categories of individuals who may be exposed to infectious organisms: susceptible, infected, diseased, immune, or dead. The potential effects of nutrition are marked on Figure Figure33 with arrows labeled with letters. Potential hosts may be particularly susceptible to infection (Arrow A) because of effects of malnutrition on non-specific defenses such as gastric acidity  and the integrity of epithelial surfaces . However a nutrient deficiency may also impair susceptibility: for example, the infectivity of epithelial cells to C. trachomatis was lower in vitro when grown in a medium containing an iron chelating compound than without, suggesting that iron is required for infection to occur .
There is considerable evidence that acquired immune responses (Arrow B), which may be full or partial, are impaired by specific micronutrient deficiencies especially of vitamin A, zinc, and iron [18,20]. Partial immunity is proposed as a reason why some individuals in longitudinal studies do not become heavily reinfected with helminths after treatment .
There is sparse evidence that undernourished hosts may become diseased because of a burden of parasites that a well-nourished host would tolerate (Arrow C), but there is evidence for some species that good nutrition can diminish the effects of parasites that might otherwise cause disease. For example a well-nourished host could replace small to moderate blood losses caused by hookworms or schistosomes, so that anemia may not occur [22,23].
There is strong evidence that undernourished hosts are in general at greater risk of dying of infectious diseases than well-nourished hosts (Arrow D), mostly based on analyses of the relationship between underweight and the risk of dying [24,25]. Examples involving NTDs are rare, largely because single infections are rare as polyparasitism is the rule rather than the exception, but also perhaps because NTDs are not a common cause of death. An experimental study in cotton rats of the filarial worm Litomosoides carinii showed a greater death rate among protein-deficient animals given the same infectious dose as well-nourished rats .
There is a little evidence, also from studies of animals, that drugs may be less effective in undernourished hosts (Arrow E), particularly animals infected with Schistosoma spp [27,28], perhaps because the immune responses necessary to kill drug-damaged worms are less effective.
The effects of undernutrition and infection outlined in Figure Figure33 may not always be for the worse, although generally they are. Undernutrition most often acts synergistically with infection, which means that the effects of both are greater or more serious for a host than would be expected from the combined effect of the two acting independently. But the joint effects may also be antagonistic, in which the combined effect is less than expected, or there may be no interaction. These concepts were developed also by Scrimshaw and colleagues in the same important review, which summarized the findings of 482 papers that had reported the relationship between nutritional deficiencies and infections with bacteria, viruses, protozoa, and helminths . In 67% of studies the relationship was judged to be synergistic, in 19% it was antagonistic and in 13% there was no effect . In nearly 80% of the 94 reported studies of helminths the relationship was judged to be synergistic, so that disease was more severe in malnourished hosts, both animal and human . An update of this review is long overdue.
The diseases listed in Table Table11 cause undernutrition by different mechanisms depending on where the organisms live and how they feed. These have recently been reviewed  and are: an increased metabolic rate (fever) as a result of inflammatory responses so that energy and nutrients are used at a greater rate than usual, for example in filarial fever or gut inflammation due to trichuriasis; increased nutrient requirements to meet the needs for immune and pathological responses to worms, larvae, or eggs, for example in filariasis and schistosomiasis; loss of nutrients due to tissue damage, for example due to hookworm or urinary schistosomiasis; malabsorption or maldigestion of nutrients, for example due to intestinal nematode worms; anorexia mediated by inflammatory cytokines released as a response to all infections; and physical debility, reduced activity, or work productivity due to chronic illness, for example due to onchocerciasis, filariasis, or trachoma.
Loss of appetite may make an important contribution to the development of undernutrition and may be exacerbated if the caregiver's response is to withhold food or, for reasons of culture or poverty, give a nutritionally poor diet . Although anorexia may be an important protective response in acute infection, the effects during repeated infection or chronic disease may eventually be harmful . Studies have shown that children's appetite and food intake increase after treating intestinal worms or schistosomiasis [32-34].
Stunted growth and underweight in children are probably among the most important consequences of NTDs, particularly due to intestinal nematode worms . There is also evidence of associations between worms and tests of cognitive function [35-37], but because both are related to poverty and a poor diet, the possibility of confounding is high. A randomized controlled trial in Jamaica of children mostly infected with Trichuris trichiura reported effects of anthelmintic treatment on tests of cognition , but more such trials are needed.
The loss of blood caused by worms has been estimated to be 0.14-0.26 mL/worm/day for A. duodenale , 0.02-0.07 mL/worm/day for N. americanus , and 0.005 mL/worm/day due to T. trichiura . The daily losses of blood caused by the eggs of S. mansoni and S. haematobium cannot be related to the number of worms but have been shown to be as much as 126.0 mL/day [41,42]. There is evidence that perhaps 40% of the iron that passes into the gut due to hookworms may be reabsorbed , but blood in urine is lost to the body.
There is no constant linear relationship between worm load and the hemoglobin concentration as the amount of iron in the diet and its absorption efficiency, which typically ranges from 5% to 25% depending on its dietary quality , can serve to counterbalance blood loss up to a point, although other micronutrients are required as well for hemopoiesis.
The main method used to assess whether NTDs have an effect on human health or growth is to undertake randomized trials of treatment, although having untreated controls is now hard to justify. The effects of treatment on most nutritional outcomes is difficult to assess, mainly because drugs kill only worms, they do not provide the nutrients needed for catch-up growth, or for any other consequent deficit for that matter. For example, if hemoglobin is being lost because of hookworms or urinary schistosomiasis then stopping that loss with an anthelmintic drug does not have an effect on the manufacture of hemoglobin in the bone marrow, that rate is mainly dependent on the quality and quantity of the diet to provide nutrients for hemopoiesis. A relative deficiency of even one micronutrient may be rate-limiting, which might explain why some studies of giving iron supplements alone to anemic people after deworming have found no effect on their hemoglobin concentration [45,46]. It also means that studies which compare different anthelmintic treatments in terms of nutritional outcomes such as hemoglobin concentration must be done in the same communities, otherwise differences could be explained by the diet, not by the drug given .
As recovery occurs only slowly for chronically undernourished individuals if their diet is not improved, supplementary food or micronutrients are usually required. For example, a study of schoolchildren in Tanzania reported an increase in hemoglobin concentration of 3.6 g/L three months after children were treated with albendazole and praziquantel alone, but the children given supplements of iron and vitamin A in addition showed an increase of 22.1 g/L over the same period . It may also be possible to achieve catch-up growth: therapeutic feeding of Jamaican children treated for Trichuris dysentery syndrome found that they grew on average 10.9 cm/year after treatment, more than two standard deviations above the rate expected of children of the same height-for-age . If no nutritional supplements are given then the rate at which body weight or the hemoglobin concentration improves depends on the degree of deficit before treatment and the diet of the people treated, not just on the drug given and its efficacy.
Although supplementary feeding programs are relatively expensive, there is potential to deliver low-cost micronutrient supplements at the same time that people are treated for NTDs, especially to school children . Table Table22 shows the current purchase costs of the major single-dose drugs used in NTD control programs and of some common nutritional interventions. The purchase costs are highly dependent on the availability of generic drugs and the number of tablets purchased, while some drugs are now available free. For example, although the nominal cost shown in Table Table22 of a tablet of ivermectin is USD 1.50, it has been made available free for treating onchocerciasis since 1987 by the Mectizan Donation Program .
As with most treatments, the major financial and economic costs arise from delivering treatments to target populations such as schoolchildren. These costs are hard to standardize or compare for a number of reasons: because methods of costing differ and may not include the costs incurred by schools ; because costs differ between countries even if calculated in a standard currency; because of inflationary effects on many costs; and because costs may diminish with economies of scale [52,53]. Nevertheless, delivering an inexpensive treatment such as vitamin A to children in the community may be about 90% of the total cost , delivering an equally inexpensive treatment such mebendazole or albendazole to schoolchildren may be around 70% of the total , while delivering biscuits to schoolchildren may be about 40% of the total . Although schools offer an existing infrastructure that could minimize the costs of delivering treatments to an often heavily infected and undernourished age group, it should not be expected that teachers will be willing to take on the responsibility of providing health services without adequate training and, perhaps, some reward. There is substantial experience that with sufficient training teachers can administer single dose anthelmintics , something that many non-governmental organizations have now been doing as a part of school health programs for almost 20 years. And randomized cluster trials have shown that teachers can effectively deliver weekly iron supplements for 3 months after deworming [56,57]. School-age children are likely to be the greatest beneficiaries from NTD control programs because they have potential to be protected from ill-health and undernutrition and have some capacity to recover before damage is substantial and permanent. They may also, as productive adults, show the quickest returns from investments in NTD control.
But drugs alone are not necessarily 'rapid impact interventions' ; they may kill worms quickly, but the recovery of any deficits they have caused will take time, especially if beneficiaries remain in the same nutritionally deprived circumstances . If volunteers in communities that suffer from NTDs are to be convinced to deliver and administer treatments then it could be important for sustainability that there are moderately quick and evident benefits. Part of the success of community directed treatment with ivermectin to treat onchocerciasis may have occurred because treatment alleviates the immediate symptoms of disease, particularly the itching skin of heavily infected adults , who tend to be a socially influential group. Studies of mass treatment for lymphatic filariasis in Tanzania indicate that because the drugs did not alleviate the symptoms of disease for adults, the program was not perceived to be useful by some beneficiaries . The value of mass treatment of filariasis lies in protecting children from damage to their lymph ducts, and is not a treatment for elephantiasis. It is also important that beneficiaries understand what they are being treated for and why, especially if they are uninfected or have no disease , something that teachers can explain to their pupils.
But most of the pathogens that are the focus of major NTD control efforts do not have specific and identifiable symptoms until disease is severe, while much of the damage to the eyes, skin, lymph ducts, liver, and kidneys is irreversible. If the effects of treating NTDs are to be quickly apparent, particularly to the mothers of children, then supplementary nutrients may be one of the simplest and least expensive interventions that there is to promote recovery after treatment and contribute to NTD control.
Although we are making a case for the value of nutritional supplements after treating NTDs, we recognize that a truly integrated control program needs more than pills. For example, it is possible to treat several NTDs at once where they co-occur ; other diseases such as malaria could be controlled at the same time [61-63]; while understanding the social determinants of reinfection  and inter-sectoral approaches  are potentially useful attributes. But the main point is that drug treatment alone is not enough, as the history of NTD control in countries such as Japan and South Korea has shown; public health measures are required as well [66,67].
Figure Figure44 illustrates a broad approach in addition to mass drug treatment, including nutritional rehabilitation, behavior change initiatives, and public health measures to prevent reinfection. Mass drug treatment will reduce the burden of disease and diminish sources of reinfection. Rehabilitation is required so that deficits in the health, nutrition, and education of children are recovered as much as possible, and symptoms of disease are alleviated in all age groups, such as entropion in trachoma and skin lesions in elephantiasis. Changes in behavior are needed to help prevent infected people from putting themselves and others at risk of reinfection. Environmental health and sanitation are essential to keep people and their excreta apart and prevent vectors and flies from breeding. And clean water can provide the means to prevent reinfection. The SAFE model of trachoma treatment and control (Surgery, Antibiotics, Face washing, and Environmental measures) offers an example for a single disease , but the challenge is to apply broad control measures to several diseases at once.
Figure Figure44 also makes the point that, as well as inputs such as scientific research, investment, a suitable policy framework, and coordination between different sectors of government, there are also important outputs. Evidence of the impact and benefits of programs will be necessary, particularly for the purposes of advocacy. Nutritional indicators such as anemia and growth are relatively easy to measure as well as being biomedically important outcomes of NTD control . The main technical problem is the need for a control group when evaluating the impact of treatment on children, because their hemoglobin concentration, weight, and height tend to increase with age anyway, albeit more slowly than if they were free of NTDs, and may be affected concurrently by seasonal influences on food supplies and disease. An impact evaluation cannot simply measure children's nutritional status before and after treatment and assume that the treatment was responsible, which is what is sometimes done . But treatments with vitamins and minerals delivered to school-age children are literally vital to build on the benefits of giving drugs to treat NTDs, and together they can help propel the current generation of children and adolescents into a sustainably healthy future.
NTD: neglected tropical disease.
The authors declare that they have no competing interests.
AH was commissioned by Helen Keller International (HKI) to write a review that led to this paper. AH drafted the paper to which all authors contributed and approved the final version.
AH is a nutritionist and parasitologist who is Visiting Reader in Public Health Nutrition at the University of Westminster, UK. YZ is a physician, parasitologist, and Africa Regional Coordinator for NTD Control for HKI. CM is Director of NTD Control for HKI, based in the USA. SB is a Vice-President of HKI and Regional Director for Africa.
The pre-publication history for this paper can be accessed here:
This review was partly funded by a grant from the Arab Gulf Program for Development. The NTD control programs of HKI are supported by donors including the United States Agency for International Development through RTI International, the Conrad N. Hilton Foundation, and Lions Club International Foundation. The paper expresses the opinions of the authors and does not necessarily reflect the official positions of these donors.