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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Am J Psychiatry. Author manuscript; available in PMC 2013 June 1.
Published in final edited form as:
PMCID: PMC3377782

Differences in White Matter Fiber Tract Development Present from 6 to 24 Months in Infants with Autism

Jason J. Wolff, Ph.D.,1 Hongbin Gu, Ph.D.,1,2 Guido Gerig, Ph.D.,1,3 Jed T. Elison, Ph.D.,1,4 Martin Styner, Ph.D.,1,2 Sylvain Gouttard, M.S.,3 Kelly N. Botteron, M.D.,5,6 Stephen R. Dager, M.D.,7 Geraldine Dawson, Ph.D.,2,8 Annette M. Estes, Ph.D.,9 Alan Evans, Ph.D.,10 Heather C. Hazlett, Ph.D.,1,2 Penelope Kostopoulos, Ph.D.,10 Robert C. McKinstry, M.D., Ph.D.,6 Sarah J. Paterson, Ph.D.,11 Robert T. Schultz, Ph.D.,11 Lonnie Zwaigenbaum, M.D.,12 and Joe Piven, M.D.1,2, for the IBIS Network*



Evidence from prospective high-risk infant studies suggests that early symptoms of autism usually emerge late in the first- or early in the second-year of life after a period of relatively typical development. This is the first neuroimaging study to prospectively examine white matter fiber tract organization during this interval in infants who develop autism spectrum disorder (ASD) by 24 months.


Participants included 92 infant siblings from an ongoing imaging study of autism. All participants had diffusion tensor imaging at 6 months and behavioral assessments at 24 months, with a majority contributing additional imaging data at either or both 12 and 24 months. At 24 months, 28 infants met criteria for ASD; 64 infants did not. Microstructural properties of white-matter fiber tracts reported to be associated with ASD or related behaviors were characterized by fractional anisotropy (FA) and radial and axial diffusivity.


FA trajectories differed significantly between infants who did versus did not develop ASD for 12 of 15 fiber tracts. Development for most fiber tracts in infants with ASD was characterized by elevated FA at 6 months followed by slower developmental change overtime relative to infants without ASD. Thus, by 24 months of age, lower FA values were evident for those with ASD.


These results suggest that the aberrant development of white matter pathways precede the manifestation of autistic symptoms in the first year of life. Longitudinal data are critical to characterizing the dynamic age-related brain and behavior changes underlying this neurodevelopmental disorder.

Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder defined by patterns of impaired social-communication and restricted, repetitive behaviors. ASD represents a significant public health concern, affecting upward of 1 in 110 children with a recurrence rate among at-risk families of nearly 1 in 5 (1, 2). Findings from prospective studies of infant siblings of children with ASD, who are at high familial risk for the disorder, indicate that a number of the defining behavioral features of ASD first emerge around 12 months of age following a period of relatively typical postnatal development (34).

Although several studies have now documented the early behavioral course of infants later diagnosed with ASD, there have been no published neuroimaging data on such infants before toddlerhood. Existing neuroimaging studies have provided evidence of significantly enlarged brain volume in two- and three-year olds with ASD using magnetic resonance imaging (MRI) (610). These findings are consistent with reports of increased brain weight in postmortem brains of autistic individuals (11), as well as numerous reports associating ASD with a general increase in head circumference (12). Two large retrospective studies suggest that this onset of increased head circumference is likely to occur in the latter part of the first year of life (7, 13). Taken together, findings from high risk infant sibling behavioral studies and studies of brain and head size growth suggest that the latter half of the first year of life is a pivotal time for both brain changes and symptom onset in infants later diagnosed with ASD. The concurrent timing of these phenomena suggests that brain changes during this period may have an important role in the pathogenesis of autistic behavior.

Autism is increasingly considered a disorder characterized in part by aberrant neural circuitry (14, 15). Functional neuroimaging studies have revealed patterns of disrupted functional connectivity in adults (16) and children (17, 18) with ASD. A growing body of work has employed diffusion tensor imaging (DTI) to gauge the microstructural properties of white matter circuitry (19, 20). DTI studies have identified evidence of widespread abnormalities in white matter fiber tract integrity among children and adults with ASD compared to controls (2125). Results from cross-sectional studies of children and adolescents with ASD (2627) have revealed both lower fractional anisotropy (FA) and less change associated with age compared to typical controls for a number of fiber tracts. Conversely, others have found increased FA and parallel age associations in young children with ASD compared to controls (28). While the preponderance of existing work suggests altered white matter development in ASD, the extent, direction, and developmental course of these differences remains unclear.

Fundamentally a disorder of development, ASD emerges early in life and is generally associated with life-long disability (29). When development itself is intrinsic to the phenomenon in question, it becomes necessary to seek answers from developmental trajectories (3032). This is particularly so in the earliest periods of infancy when dramatic changes in behavior are paralleled by dramatic changes in the brain. White matter pathways, for instance, rapidly develop during the first two years of life, after which change is attenuated (35). To date, however, there are no longitudinal studies of neural circuitry in ASD, and but a few concerning volumetric brain development (8, 33, 34). Although numerous cross-sectional studies of children with ASD posit key neurological changes relevant to the disorder, few employ a true developmental perspective. As Karmiloff-Smith (36) aptly observes, cross-sectional neuroimaging studies of children are not tantamount to neuroimaging studies of development.

We present here the first prospective, longitudinal study of early postnatal white matter fiber tract development at 6, 12, and 24 months of age in infants who are at high familial risk for ASD by virtue of having an older sibling with the disorder. In this study, we compare the subset of high risk infants who show evidence of ASD by 24 months of age to those who do not. Given that both groups have a higher familial liability for ASD than the general population, this design allows for inferences not afforded to comparisons of high risk children with ASD to children at low familial risk. The focus of this study is on the development of white matter pathways selected on the basis of reported associations with ASD or core behavioral features of the disorder (2128).



This study includes data from an NIH-funded Autism Center of Excellence (ACE). The parent ACE Network is an ongoing brain imaging study of infants who are at risk for autism. Four clinical data collection sites are associated with the study: University of North Carolina at Chapel Hill, University of Washington in Seattle, Children’s Hospital of Philadelphia, and Washington University in St. Louis. All data were coordinated through the Montreal Neurological Institute at McGill University, and data processing performed at the University of North Carolina and the SCI Institute at the University of Utah. The parent study enrolled and assessed 6 month old high-risk (HR) infants who were also seen for follow-up assessments at 12 and 24 months of age. Written informed consent was obtained from the parent or legal guardian of each participant prior to enrollment.

Exclusionary criteria included the following conditions: (1) significant medical conditions known to affect brain development, (2) sensory impairments, (3) low birth weight (<2200g) or prematurity (<36 weeks gestation), (4) evidence of perinatal brain injury secondary to maternal complications or exposure to specific medications or neurotoxins (e.g., alcohol) during gestation, (5) non-English speaking families, (6) evidence for contraindication for MRI (e.g., metal implants), (7) adopted children, and (8) family history of first degree relative with idiopathic intellectual disability, psychosis, schizophrenia, or bipolar disorder.

The present sample included all HR infant siblings with diffusion-weighted MRI scans at 6 months who had completed behavioral assessments at 24 months of age as of June 2011. Symptoms of ASD were measured at 24 months using the Autism Diagnostic Observation Schedule (37) by research-reliable administrators to maximize agreement across sites. HR infants were divided into two groups based on ADOS classifications: HR(−) (below ASD cutoff) and HR(+) (above ASD cutoff). The Mullen Scales of Early Learning (38) was administered at each visit and Early Learning Composite (ELC) scores at 24 months of age used to characterize general developmental level among the sample. Four subjects (2 HR− and 2 HR+) were missing complete Mullen data at 24 months and 12 month Mullen ELCs were substituted. Descriptive and demographic data are presented in Table 1.

Characteristics of High-Risk Infants with and without Evidence of ASD at 24 Months Age

Image Acquisition

MRI brain scans were completed at each clinical site on identical 3T Siemens TIM Trio scanners equipped with 12-channel head coils during natural sleep. The DTI sequence was acquired as an ep2d_diff sequence with FoV = 190 mm (6 and 12 months), and FoV = 209 mm (24 months), 75–81 transversal slices, slice thickness = 2 mm isotropic, 2×2×2mm3 voxel resolution, TR = 12800–13300 ms, TE = 102 ms, variable b-values between 0 and 1000 s/mm2, 25 gradient directions and scan time of 5–6 minutes. Intra- and inter-site reliabilities were initially established and regularly evaluated via a human traveling phantom study (39).

Image Pre-processing

DWI image data were screened for subject motion and common artifacts related to diffusion sequences using the DTIprep software (40). This tool automatically detects artifacts, corrects for motion and eddy current based deformations, and excludes individual volumetric DWI images with artifacts and generates a full report. Expert raters manually removed DWI scans with clear, residual artifacts. Datasets with less than 18 (70%) gradient DWI images remaining after this quality procedure were excluded from further processing and analysis due to the resulting reduction in signal-to-noise.

DTI Processing Pipeline

The group analysis of DWI data, here processed via diffusion tensor estimates and analysis, employed a recently developed processing pipeline (41). This processing overcomes the major challenge of implementing tract oriented statistics in large study populations, which is finding a consistent spatial parametrization within and between populations. The pipeline includes a computational anatomy approach for nonlinear co-registration of all DTI data to a template reference coordinate frame, a fiber tract oriented process to parameterize tracts to functions of tract length, and mapping of individual tract geometries into common tract coordinates.

Computational Anatomy Mapping

Unbiased atlas building (42) was used to provide a one-to-one mapping between individual subjects’ image data and the template atlas, where the atlas reference image is built from the population of image data as the centered image with least deformation distances. The registration proceeds in two steps (41). A first step applies linear, affine registration of the DWI baseline images to a structural weighted T2 atlas using B-spline registration and normalized mutual information (43). This is followed by an unbiased, deformable atlas building procedure (42) that applies large deformation diffeomorphic metric mapping transformations. The procedure relates each individual dataset to the study-specific atlas template space via a nonlinear, invertible transformation. Tensor maps were calculated from each DWI dataset using weighted least squares estimation, and transformed into the atlas space with tensor re-orientation by a finite strain approach (44). The transformed DTI tensor images are averaged using the Riemannian framework (45), resulting in the final 3D average tensor atlas to be used as the reference frame for tractography and tract parameterization. For this study, the longitudinal DWI data of each subject covering the age range of 6 to 24 months were processed with the procedure described above, with all subjects at all ages mapped into the same atlas space.

Fiber Tractography

Seed label maps were created based on methods described in existing tractography atlases (46, 47) and drawn in the combined atlas for regions of interest (ROI) using 3D Slicer ( A secondary check of ROIs was made using the early childhood DTI atlas (35). Label maps were created for the following fiber tracts: the genu, body, and splenium of the corpus callosum (CC), fornix, inferior longitudinal fasciculus (ILF), uncinate fasciculus (UF), anterior thalamic radiations (ATR), and the anterior and posterior limbs of the internal capsule (ALIC and PLIC). Label maps were created bilaterally for all tracts with the exception of the CC. Fiber tracts generated in 3D Slicer were processed for spurious or incomplete streamlines using open-source software developed in-house (FiberViewer; Fractional anisotropy (FA) values were generated for each fiber tract. FA is an index measuring the degree of anisotropy of local diffusivity, ranging from 0 for isotropic diffusion in fluid to 1 for strongly directional diffusivity in highly structured axonal bundles (18, 19). Axial (AD; λ1) and radial [RD; (λ2, + λ3)/2] diffusivity values, which represent diffusion parallel and transverse to axonal directions, were also produced for each fiber tract.

Statistical Analysis

Demographic characteristics were compiled for HR(+) and HR(−) groups at 6, 12 and 24 month time points. Potential group differences were tested for age, sex, and Mullen ELC at each time point using either t-tests (age and Mullen score) or Fisher’s exact test (sex).

Longitudinal trajectories of mean FA values for each fiber tract were compared between HR(+) and HR(−) groups using random coefficient linear growth curve models. The random coefficient model is able to fit a group developmental trend while accounting for variability in individual growth trajectories. Among the 92 HR subjects included for the analysis, 14 subjects had 1 visit; 40 had 2, and 38 had 3 visits. The mixed -model framework is able to accommodate different missing patterns and unbalanced designs.

Although white matter is known to develop more rapidly in the first year of life than in the second, a linear model was determined to best fit FA change across time given the limited number of available time points. Separate growth curve models were fit for each fiber tract with age, group, and group X age interaction as fix effects, and age intercept and slope as random effects. All growth curve models included 24 month Mullen ELC scores as a covariate. Following interaction findings between age X group, separate growth slopes were estimated for HR(+) and HR(−) groups. Least square mean FA’s were estimated for each group and contrasted at 6, 12, and 24 months to highlight differences at each individual time point. To fully characterize FA results, axial and radial diffusivity were examined in a secondary analysis using the above described growth model. Because of the preliminary nature of the present stage of analysis, family-wise alpha was uncorrected for multiple comparisons.

All analyses were done using SAS software (SAS 9.2, Cary, NC).


There were no differences between HR(−) and HR(+) groups in age or sex ratio at 6, 12, or 24 month scans (p > .10), or age at 24 month behavioral assessment. Mullen ELC scores were approximately 12 points lower in the HR(+) group than HR(−) in samples at each time point: 6 months, t(1, 90) = 2.8, p = .007; 12 months, t(1, 64) = 2.5, p = .01; and 24 months, t(1, 47) = 2.1, p = .04. Descriptive and demographic data are presented in Table 1.

Slope parameter estimates with standard error are presented for HR(+) and HR(−) groups for all tracts in Table 2. Both HR(−) and HR(+) groups showed significant increases in FA from 6 to 24 months, but the rate of change for the HR(−) group was significantly greater than that for HR(+) for bilateral limbic (fornix) and association (ILF and uncinate) fiber tracts. Individual and mean group trajectories for these tracts are presented in Figure 1. For corpus callosum subdivisions (Figure 2), change in FA from 6 to 24 months was significantly different for the body of the CC. For projection tracts (ATR, ALIC, and PLIC), growth trajectories of the left ATR and bilateral ALIC and PLIC were significantly steeper for HR(−) infants (Figure 3).

Trajectories of Mean Fractional Anisotropy for High-Risk Groups, Limbic (Fornix) and Association (ILF and Uncinate) Fiber Tracts
Trajectories of Mean Fractional Anisotropy for High-Risk Groups, Corpus Callosum Subdivisions
Trajectories of Mean Fractional Anisotropy for High-Risk Groups, Projection Fiber Tracts
Linear Growth Model Estimates for Monthly Mean FA Change Rate (×10−3)

Follow-up cross-sectional analyses for mean FA are presented in Table 3. Mean FA was significantly higher for HR(+) versus HR(−) at the 6 month time point for the left fornix, t(89) = 2.1, p = .04; the left ILF, t(89) = 2.6, p = .01; and left uncinate, t(89) = 2.2, p = .03. Mean FA for the body of the CC was significantly higher in the HR(+) group at 6 months, t(89) = 2, p = .04. Mean FA for the right PLIC was significantly higher in the HR(+) group at 6 months, t(89) = 2.1, p = .04; mean FA for the left ALIC was significantly higher in the HR(−) group at 24 months, t(89) = −2.1, p = .04. FA for the left ATR was significantly higher in HR(−) at both 12 [t(89) = −2, p = .04] and 24 [t(89) = −3, p = .003] months.

Least-Square Mean Estimates for Group Mean FA Difference (×10−3) at 6, 12, and 24 Months

Results from secondary analyses of axial and radial diffusivities are presented in Table 4. Linear growth trajectories did not differ significantly for these indices of tract diffusion with the exception of the PLIC, for which RD was significantly higher across the 6 to 24 month interval for the HR(+) group.

Linear Growth Model Estimates for Monthly Mean AD and RD Change Rates (×10−6)


This study represents the first prospective, longitudinal imaging study of infants at-risk for ASD. The findings reveal a distinct and pervasive course of aberrant white matter fiber tract development in HR infants who go on to develop autistic symptoms. Trajectories of fractional anisotropy (FA) values for 12 of the 15 tracts examined in the present study differed significantly between groups. Most fiber tracts for HR(+) infants were characterized by higher FA at 6 months followed by blunted developmental trajectories such that FA was lower by 24 months. Around 12 months of age, HR groups appear similar across all tracts excepting the ATR, potentially indicating differential timing of development for this specific fiber bundle. Understanding why FA values for multiple fiber tracts are elevated at 6 months but show less change over time may be critical to understanding the development of ASD and shed light on the neural mechanisms underlying its onset.

In agreement with findings from typically developing infants, FA values for HR(−) infants were characterized by rapid change during the 6 to 24 month interval (35). The blunted trajectories seen in the HR(+) group are consistent with the reduced FA and comparative absence of age-related associations observed in older children and adolescents with ASD (24, 26, 27), although these patterns may vary by fiber pathway throughout development (25, 28). The differences in white matter development between HR groups observed in this study are particularly striking considering evidence that individuals with ASD and nonaffected family members of individuals with ASD share a neural phenotype consisting of specific structural and functional brain abnormalities (48, 49). In follow-up analysis, we examined axial and radial diffusivities to elucidate FA results. With a few exceptions, trajectories for these diffusivity measures were not significantly different between groups, suggesting that FA results stem in part from the proportional relationship of AD and RD and not from one measure alone.

The altered trajectories of development seen here ostensibly begin in advance of both the onset of clinical symptoms (35) and volumetric changes (6, 8), suggesting that core behavioral features of ASD arise from an altered neurobiological foundation. Instantiated by the present results, the organization of neural networks underlying ASD is characterized by atypical patterns of connectivity that differ across systems and time (1417) and are not specific to any one brain region or behavioral domain. The relevance of developmental trajectories to understanding these dynamic processes is undeniable (3032). Had the present study included only cross-sectional data centered at 12 months, for instance, it might have followed that the ATRs are uniquely relevant to the early development of ASD. The wider view afforded by longitudinal data indicate that the ATRs are but one of many fiber tracts implicated in the disorder, with associations at 12 months the product of differential development.

Both highly experience-dependent and less environmentally mediated processes contribute to functional and structural organization of the brain, and the dynamic interplay of these processes over time yield specialized cortical circuits designed to optimally process complex information (50). For example, differences in structural organization prior to a period of experience-dependent developmental processes related to social-cognition (5153) may decrease neural plasticity through limitations on environmental input, preventing typical neural specialization (50). These alterations could have a ripple effect through decreasing environmental responsiveness and escalating invariance, thus canalizing a specific neural trajectory resulting in the behavioral phenotype that defines ASD. In typical development, the selective refinement of neural connections via axonal pruning (54), along with constructive processes such as myelination (55), combine to yield efficient signal transmission among brain regions. One or both of these mechanisms may underlie the widespread differences in white matter fiber pathways observed in the current study. Intriguingly, our data is consistent with recent evidence from postmortem stereological research (56) and mouse models of autism (57) suggesting that axonal plasticity may be implicated in the development of ASD.

It is clear that the neurodevelopmental story of ASD neither begins at 6 months of age nor ends at 24. Downward extending neuroimaging to infants prior to 6 months would help clarify the temporal origin of diverging trajectories, while extending to later ages would capture increasingly stable neurobehavioral outcomes. Additional data points would likewise refine the calibration of trajectories beyond linear models. A multimodal approach to neuroimaging could potentially sharpen our understanding of early brain changes in ASD, allowing for the investigation of functional and structural covariance. To better understand the mechanisms which engender and maintain the trajectories evidenced here, subsequent research might also consider the role of genetic and epigenetic variables in the development of structural neural circuitry.

Finally, the presence of significant differences in FA at 6 months raises the exciting possibility of developing imaging biomarkers for risk of ASD in advance of symptom onset. Future work might investigate the potential of predictive models for ASD in early infancy, a process which could include refined imaging techniques or combined bio-behavioral markers. Identifying infants at highest risk for ASD before the full syndrome is manifest offers the possibility of implementing behavioral and other interventions during infancy that could reduce or even prevent the manifestation of the full syndrome (58).

There are several limitations to the present study. The absence of a low-risk contrast group limits interpretation of results outside of a familial background for ASD. Although changes in FA correspond to organizational properties indicative of development (19, 35) as well as histological findings (59), it is an imperfect index of white matter microstructure, and may reflect the effects of axonal packing, crossing fibers, or partial volumes related to noise in DTI data (20, 60). In this study, ASD status was based on ADOS scores at 24 months of age. Follow-up diagnostic assessment at 36–48 months will provide greater assurance of diagnostic outcomes.

This study is the first to identify brain changes related to later ASD as early as 6 months of age. The aberrant development of multiple white matter pathways seen here, along with previously reported brain and behavioral change during infancy (39), suggest a period of critical importance to the pathogenesis of ASD. A developmental approach to understanding neural and behavioral changes during this time, sensitive to characterizing longitudinal trajectories, is crucial to understanding the complexities inherent to the neurodevelopmental processes implicated in the emergence of ASD.


This work was supported by grants from the National Institutes of Child Health and Development, R01 HDO55741, HD055741-S1, P30 HD03110, and T32 HD40127; Autism Speaks, and the Simons Foundation. Further support was provided by the National Alliance for Medical Image Computing (NA-MIC), funded by the NIH through grant U54 EB005149.

We wish to thank our IBIS children and families for their ongoing participation in this longitudinal study. Thanks also to Varun Puvanesarajah and Eric Maltbie for assisting with tractography, and Rachel G. Smith, Cheryl Dietrich, and Mahshid Farzinfar for the DWI/DTI correction and QC efforts.


Competing interests: All study authors report no financial relationships with commercial interests.


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