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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nano Lett. Author manuscript; available in PMC 2013 June 13.
Published in final edited form as:
PMCID: PMC3374901
NIHMSID: NIHMS379643

Outside Looking In: Nanotube Transistor Intracellular Sensors

Abstract

Nanowire-based field-effect transistors (NWFETs), including devices with planar and three-dimensional (3D) configurations, are being actively explored as detectors for extra- and intracellular recording due to their small size and high sensitivities. Here we report the synthesis, fabrication and characterization of a new needle-shaped nanoprobe based on an active silicon nanotube transistor, ANTT, that enables high-resolution intracellular recording. In the ANTT probe, the source/drain (S/D) contacts to the silicon nanotube are fabricated on one end, passivated from external solution, and then time-dependent changes in potential can be recorded from the opposite nanotube end via the solution filling the tube. Measurements of conductance versus water-gate potential in aqueous solution show that the ANTT probe is selectively gated by potential changes within the nanotube, thus demonstrating the basic operating principle of the ANTT device. Studies interfacing the ANTT probe with spontaneously beating cardiomyocytes yielded stable intracellular action potentials similar to those reported by other electrophysiological techniques. In addition, the straightforward fabrication of ANTT devices was exploited to prepare multiple ANTT structures at the end of single probes, which enabled multiplexed recording of intracellular action potentials from single cells, and multiplexed arrays of single ANTT device probes. These studies open up unique opportunities for multisite recordings from individual cells through cellular networks.

Keywords: core/shell nanowires, nano-bioelectronics, nanoelectronic device, nanosensor, intracellular action potentials

An electronic device that can interface to the intracellular region of a live cell has several important constraints, including (i) small size, which can minimize invasiveness and potentially allow contact to subcellular structures, (ii) high sensitivity with decreasing size, and (iii) capability to multiplex at both single cell and cell network levels.1,2 Well-established electrophysiological techniques for cellular recording, such as patchclamp micropipettes,3 metal microelectrodes4 and intracellular glass microelectrodes,5,6 have advanced the understanding of electrogenic cells, although also have limitations in terms of (i) decreasing signal and signal-to-noise (S/N) with decreasing probe size in the submicron regime and (ii) capabilities for multiplexed measurements on single cells as well as larger-scale multiplexing for cell networks. Recently, researchers have been addressing these issues with the development of novel transistor-based probes7-9 and improved designs for micro/nanoelectrodes.10-12 The micro/nanoelectrodes exploit structures projecting from chip plane to enable invagination of cultured cell membranes and measurements of intracellular-like action potentials,10-12 although as passive recording electrodes they are limited in terms of their potential for miniaturization. On the other hand, transistor-based probes can be reduced in size to at least the 10nm scale.1,13 However, active transistor requires two contacts for operation, and until recently this constraint has made intracellular recording devices difficult to achieve. Our group has recently described two solutions to this conundrum involving the synthesis of (i) kinked NWFETs with a transistor or diode at the tip, where the nonlinear NW structure enables ready insertion into cells,7,9 and (ii) branched-nanotube NWFETs, where the transistor remains outside of the cell but senses the intracellular potential via the solution inside the passive nanotube bridge.8

Here we demonstrate a conceptually new and practically simple field-effect transistor based probe that consists of a single semiconductor nanotube. The underlying principle of the active nanotube transistor, ANTT, intracellular probe (Figure 1A) involves the fabrication of S/D contacts to one end of a silicon or other semiconductor nanotube and electrical isolation of these S/D contacts from surrounding medium such that the solution filling the interior of the nanotube can gate the transistor and the variation of interior electrochemical potential is then recorded as a change in device conductance. Hence, if the free end of an ANTT probe is inserted into the interior of an electrogenic cell, the time-dependent changes associated with an action potential spike will give rise to time-varying conductance signal that maps the intracellular action potential. However, if a similarly configured solid silicon (or other semiconductor) nanowire is inserted into the cell, no signal would be observed since it would not be possible to ‘gate’ the transistor. We note that for p-type silicon nanotubes as used in our proof-of-concept studies, a positive change of intracellular potential yields a negative change in device conductance (Figure 1A), although the conductance can be quantitatively converted to potential using water-gate calibration measurements.1,7-9

Figure 1
Principle and fabrication of the ANTT probe

The fabrication of ANTT probes was carried out in several steps briefly illustrated in Figure 1B.14 First, Ge/Si core/shell NWs were synthesized by nanocluster catalyzed vapor-liquid-solid (VLS) growth of Ge NWs followed by p-type Si shell deposition as described previously.15-18 The Ge/Si NWs were dispersed from isopropyl alcohol or contact printed19-22 onto a prebaked SU-8 layer, which was initially deposited on a sacrificial nickel relief layer. After defining the lower SU-8 passivation/isolation layer by electron beam lithography (EBL), S/D metal contacts (Cr/Pd/Cr, 1.5/75/50 nm) followed by an upper SU-8 passivation/isolation layer were patterned by EBL. Etching the nickel sacrificial layer and Ge core of the Ge/Si NW yielded the p-type Si ANTT probe (Figure 1C). Scanning electron microscopy (SEM) images of ANTT probes (Figure 1D) confirm the basic fabrication strategy and show clearly the nanotube structure. Comparison of SEM images of ANTT device before and after the Ge core etching (Figure S1) show clearly the open tip and a ‘bright-to-dark’ change in image contrast along the length of the structure that is indicative of an open nanotube from the tip through to the S/D metal contacts. The “bend-up” angles, typically 40-60° and height, usually 15-30 μm in our design, are controlled by changing the length of the stressed bimetallic S/D arms as described previously.7

We have investigated the electrical properties of the probe structures fabricated in this manner in aqueous solution before and after etching the Ge NW cores. Prior to Ge-etching, conductance (G) versus water-gate potential (Vwg) data show only a small change with a sensitivity of ~0.10 μS/V (Figure 2A). Notably, after etching the Ge core to form the ANTT device the water-gate data exhibits a 30× sensitivity increase to 3.0 μS/V.23 These results are consistent with the increased gate coupling afforded by solution access to the Si-nanotube interior. To rule out the possibility that the Ge-etching process degrades the SU-8 passivation, we carried out control experiments on a Si/Si intrinsic-core/p-shell NW structure, where the shell is similar to the Ge/Si core/shell NWs used to make ANTT devices. Significantly, the Si/Si control device with SU-8 passivated S/D contacts showed similar sensitivity to the Ge/Si device and little or no sensitivity change following the same etching conditions used to remove Ge and yield the ANTT device. Taken together these results show that (1) the top/bottom SU-8 passivation effectively isolates the FET channel from external solution potential changes, and (2) the ANTT structure has good sensitivity to potential changes coupled through solution in the nanotube interior.

Figure 2
Potential and chemical sensitivities of ANTT devices

In addition, we have explored the capability of the ANTT device to detect chemical changes in pH sensing experiments. Measurements of the G as a function of stepwise increasing pH showed a systematic increase with the increasing negative charge on the SiO2 surface24 of the p-type nanotube interior. These data plotted as change in potential versus solution pH (Figure 2B) yield a device pH sensitivity of up to 37 mV/pH, a value that is consistent with previous results reported for Si NW devices.7 Measurements made before and after closing the nanotube device end with SU-8 (inset, Figure 2B) confirm that this change is indeed due to pH detection from the solution inside the nanotube as no variation in G was observed after blocking solution exchange. These results show the potential of the ANTT probe to detect chemical and biochemical changes as previously demonstrated by nanowire FET sensors,7,24 although we note that necessity for diffusion of analytes within the nanotube will reduce the temporal resolution compared to external surface binding.

We explored the possibility of recording intracellular action potential with the ANTT probes using spontaneously firing chicken cardiomyocytes. In these experiments, ANTT probes relieved from the surface (e.g., Figure 1D) were modified with a phospholipid layer,7-9,25 cardiomyocytes were cultured on flexible sheets of polydimethlylsiloxane (PDMS) as described previously,25,26 and then the PDMS/cell sheet was moved to orient a single cell over an ANTT probe tip with an optical microscope. After contact with the cell, we initially observed regularly spaced spikes with a frequency of 1.8 Hz (Figure 3A) and correlated with cell beating. These peaks detected initially after contact had widths of ~0.7 ms and amplitudes up to 10 mV that are consistent with extracellular cardiomyocyte action potentials reported previously.1, 7, 26

Figure 3
Action potential recording with ANTT probes

Notably, over a period of ca. 100 seconds following contact between the ANTT probe tip and PDMS-supported cell the recorded periodic signals change substantially (Figure 3B) with an increase in amplitude and duration to 40-50 mV and ca. 200 ms respectively. Over a period of several minutes the peak amplitude continues to increase until stable periodic peaks are observed (Figure 3C) with amplitude and duration of ca. 80 mV and 200 ms, respectively. The peak duration and shape were similar to values reported for cardiomyocyte intracellular action potentials,27-29 and thus we can associate these data with intracellular action potential recording by the ANTT probe. Indeed, closer examination of a representative steady-state recorded peak (Figure 3D) shows five phases that can be associated with (1) resting state, (2) rapid depolarization, (3) plateau, (4) rapid repolarization, and (5) hyperpolarization, as discussed previously.7 We also note that preliminary experiments carried out with a smaller inner/outer diameter (15/50 nm) ANTT probe (Figure S2) showed similar intracellular action potential peaks with ca. 75 mV amplitude, consistent with the size-scaling predicted for the related BIT-FET.8

In addition, we have investigated two directions in multiplexed recording with the ANTT probe, including (1) multiple ANTT devices on a single probe and (2) arrays of ANTT probes. First, we fabricated probes with two ANTT devices as shown schematically in Figure 4A. Key steps in the fabrication involved contact-printing21 of the Ge/Si core/shell NWs to produce parallel NWs, and the use of a common source contact for both devices; the remainder of the fabrication process was similar to that described above.14 A representative SEM image (Figure 4A) shows clearly two ANTT devices on the single 3D probe with a nanotube tip-to-tip separation of ca. 7.6 μm. Notably, measurements made from two ANNT devices on a single probe following contact with one beating cardiomyocyte (Figure 4B) demonstrated regular intracellular action potential peaks from both devices. At steady-state the peaks recorded by both ANTT devices exhibited potential changes of about 80 mV consistent with intracellular action potentials and results reported recently for a two-element BIT-FET.8 Examination of the data recorded by both devices shows other important features. First, the noise in both channels is not correlated, and thus we can conclude that there is no significant electrical crosstalk between the dual-device/single-probe configuration even when recording from the same cell. Second, the S/N for one device (top trace, Figure 4B) is lower than that for the second device on the probe, which represents a limitation that will need to be improved in future for exploiting the potential of high spatial resolution recording using these multiplexed ANTT probes.

Figure 4
Multiplexed ANTT probes

Last, we have also extended our approach to fabricated arrays of ANTT probes. For example, a 4 × 4 array of single ANTT probes, with an average probe spacing of 80 μm, was fabricated from contact-printed Ge/Si NWs (Figure 4C). Denser ANTT probe arrays could be fabricated by varying the printing conditions.30 In addition, we are also exploring a strategy that involves epitaxial growth of ca. vertical Ge/Si NWs to produce high-density ANTT probe arrays (Figure 4D).31 In this approach, S/D contacts are defined vertically to the vertical semiconductor nanotubes in a manner similar to work on vertical nanowire FETs.32,33

In conclusion, we have demonstrated the design, synthesis, fabrication and testing of a new hollow needle-shaped nanoprobe based on an active silicon nanotube transistor termed the ANTT device. Measurements of conductance versus water-gate potential in aqueous solution have shown that the ANTT probe is selectively gated by potential change within the silicon nanotube, thus demonstrating the basic operating principle of the ANTT device. Studies interfacing ANTT probes with spontaneously beating cardiomyocytes demonstrated the recording of stable full-amplitude intracellular action potentials, and moreover, showed that full-amplitude action potentials could be recorded with inner tube diameters as small as 15 nm. In addition, the straightforward fabrication of ANTT devices was exploited to prepare multiple ANTTs at the end of single probes, which enabled multiplexed recording of full-amplitude intracellular action potentials from single cells, and multiplexed arrays of single ANTT device probes. These studies open up unique opportunities for multisite recordings from individual cells through cellular networks, including the potential for intracellular chemical sensing through modification of the inner tube surface.34

Supplementary Material

1_si_001

Acknowledgements

The authors acknowledge J. Huang and D. Bell for helpful discussions. R. G. acknowledges a Japan Student Services Organization Graduate Research Fellowship. S. S. acknowledges financial support by the German Research Foundation (STR1119/2). C.M.L. acknowledges support from a NIH Director‘s Pioneer Award (1DP1OD003900).

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