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Table 1

Strains used in this study

StrainGenotypeSource or reference
CG1hL972M. Sipiczki (University of Debrecen)
CG10hmed13+::TAP-kanMX6 ade6-21619
CGG15h+cdc25-22M. Sipiczki (University of Debrecen)
CG27h+med7+::TAP-kanMX6 med13::kanMX419
CG39h+cdk8-D158A19
CG45hcdc25-22 med7+::myc-kanMX6This study
CG94h+ cdk8-D158A cdc25-22 med7+::myc-kanMX6This study
CG117h+ fkh2-3HA::kanMX6 cdc25-2212
CG119hcdk8-D158A cdc25-22 fkh2-3HA::kanMX6This study
CG134h+ fkh2::1FLAG-nat-MX6 cdc25-22This study
CG152h+ cdc25-22 cdk8-D158AThis Study
CG120h+fkh2::kanR cdc25-22 med7+::myc-kanMX6This study
CG175h+ cdk8-D158A fkh2::kanMX4 cdc25-22This study
CG186hfkh2-3HA::kanR mts3-1 ura4-D18 leu1-32 ade6-M210C. Gordon (MRC, Edinburgh, United Kingdom)
CG207hcdc25-22 fkh2-S322A S375A-3HA::nat-MX6This study
CG206hfkh2-S322A S375A-3HA::nat-MX6This study
CG209hfkh2-3HA::kanR12
CG271h+fkh2-S322E S375E-3HA::nat-MX6 ade6-MxThis study
CG283hfkh2-S322E S375E-3HA::nat-MX6 cdc25-22This study
CG284h+fkh2-S322E S375E-3HA::nat-MX6 wee1-50This study
CG211h+ wee1-50 cdk8-D158AThis study
CG215hwee1-50 fkh2-S322A S375A-3HA::nat-MX6This study
CG181MATaURA3::lexA-lacZ met- his3 ade2 trp1 leu2 gal4D gal80D43
CG-336h+cdc25-22 cdk8-3HA::natMX6This study
CG-236hnda3-KM311 fkh2-3HA::kanRThis study
CG-340hnda3-KM311 fkh2-322A 375A-3HA::nat-MX6This study
CG-341h+nda3-KM311 fkh2-S322E S375E-3HA::nat-MX6This study
CG-344hmts3-1 fkh2-322A S375A-HA::natMX6This study
CG-349hcdk8-D158A fkh2-S332A 375A-3HA::nat-MX6This study
CG-350h+fkh2-PESTmut-HA::nat-MX6 ura4-D18 leu1-32This study