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Lysyl Oxidase (LOX, also called protein-lysine 6-oxidase) (Genbank accession numbers: Nucleotide NM_002317.5, Protein NP_002308.2, EC 184.108.40.206) is one of the five LOX family members (LOX and LOX Like1-4). The human LOX gene is located on chromosome 5q23.2. LOX is synthesized as a 50 kDa pre-protein containing 3 domains; the N-terminal signal peptide sequence (aa1–21), the N-terminal pro-peptide domain (aa 22–162), and the C-terminal catalytic domain (aa 162–417). The signal peptide is removed by cleavage at the Cys21-Ala22 bond and the pro-peptide domain is N-glycosylated in the endoplasmic reticulum and Golgi apparatus yielding a pro-enzyme, which is then secreted from cells as a catalytically inactive protein. The 32 kDa active enzyme (C-terminal domain) is released by proteolytic cleavage of the pro-peptide at Gly162–Asp163 by procollagen C-proteinase (bone morphogenetic protein 1; BMP-1). (Kagan and Li, 2003).
LOX belongs to a family of amine oxidases. LOX was initially reported to be expressed and secreted by fibrogenic cells but is now known to be expressed in several other cell types. LOX oxidizes peptidyl lysine and hydroxylysine residues in collagen and lysine residues in elastin to produce peptidyl alpha-aminoadipic-delta-semialdehydes. These aldehyde modifications can spontaneously combine with vicinal peptidyl aldehydes or with epsilon-amino groups of peptidyl lysine to form covalent cross-links that stabilize and cause collagen and elastin fibers to be insoluble in the extracellular matrix (ECM). The significance of LOX was demonstrated in the LOX knockout mouse, which could not survive at birth, due to rupture of the aorta and diaphragm from incomplete elastin cross-linking. LOX is also essential for development of the distal and proximal airways, and the formation of alveoli in the lungs. LOX is also critical for notochord formation and muscle development. LOX affects differentiation of osteoblasts by forming cross-links in the surrounding collagen matrix. These results underline the importance of LOX in the morphogenesis and repair of connective tissues of the cardiovascular, respiratory, skeletal, and other organ systems (Fong SFT et. al., 2009). LOX can also induce crosslinking between lysines in histones in the nucleus (Kagan and Li, 2003). LOX has also been reported as a potent chemotactic agent for monocytes and vascular smooth muscle cells.
Due to the range of LOX biological functions, abnormalities of LOX expression underlie the development of a number of pathological processes related with an imbalance in ECM synthesis/degradation. These include connective tissue fibrotic disorders of the heart (myocardial fibrosis), vasculature (arterial fibrosis), lungs (pulmonary fibrosis), skin (keloids, and scleroderma), kidney (nephritis), liver (liver stiffness preceding liver fibrosis), mouth (gingival atrophy), and colon (intestinal fibrotic disease). LOX is also associated with diseases such as rheumatoid arthritis, systemic sclerosis, Alzheimer’s disease, and several stages of breast cancer. (Rodriguez et al., 2008).
LOX is expressed in several ocular tissues, and the following diseases of the eye have been linked with LOX:
With most of the studies in eye research focusing on the genetic association of SNPs in LOXL1 gene in pseudoexfoliation syndrome, new findings of LOX and other LOX genes are still emerging. Compared to other systems, our understanding of LOX in the eye is very limited. Several questions need to be addressed in future studies. (1) Which LOX and LOXL genes play a role in ocular homeostasis and pathologies? (2) What cellular signaling mechanisms regulate expression of LOXs in ocular tissues? (3) Does LOX or other LOX genes regulate IOP and if so, what is the mechanism of this regulation? (4) Does LOX modulate physiology of other ECM-rich ocular tissues like optic nerve head? (5) Does altering LOX activity in cultured corneal epithelial cells regulate cellular elastin levels and what factors regulate LOX in keratoconus? (6) The cause and effect between LOX levels and RRD pathology should be evaluated. (7) How does high glucose affect LOX mRNA, protein, and enzyme activity in cultured retinal epithelial cells? (8) Will modulating ocular expression of LOX genes serve as a future therapy?
The authors would acknowledge Matthew Evans at UT Southwestern Medical Center for his assistance with generating 3D LOX structure. The authors would also like to acknowledge grant support from the National Institute of Health-National Eye Institute (EY-017374 awarded to RJW).
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