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**|**Biomed Opt Express**|**v.3(6); 2012 June 1**|**PMC3370969

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Biomed Opt Express. 2012 June 1; 3(6): 1279–1290.

Published online 2012 May 8. doi: 10.1364/BOE.3.001279

PMCID: PMC3370969

Anthony N. Kuo,^{1,}^{*} Ryan P. McNabb,^{2} Mingtao Zhao,^{2} Francesco LaRocca,^{2} Sandra S. Stinnett,^{1,}^{3} Sina Farsiu,^{1,}^{2} and Joseph A. Izatt^{2,}^{1}

Received 2012 March 6; Revised 2012 May 4; Accepted 2012 May 4.

Copyright ©2012 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

This article has been cited by other articles in PMC.

We present a comparison of corneal biometric values from dense volumetric spectral domain optical coherence tomography (SDOCT) scans to reference values in both phantoms and clinical subjects. We also present a new optically based “keratometric equivalent power” formula for SDOCT that eliminates previously described discrepancies between corneal power form SDOCT and existing clinical modalities. Phantom objects of varying radii of curvature and corneas of normal subjects were imaged with a clinical SDOCT system. The optically corrected three-dimensional surfaces were used to recover radii of curvature and power as appropriate. These were then compared to the manufacturer’s reference values in phantoms and to measurements from topography and Scheimpflug photography in subjects. In phantom objects, paired differences between SDOCT and reference values for radii of curvature were not statistically significant. In subjects, there were no significant paired differences between SDOCT and reference values from the other modalities for anterior radius and corneal keratometric power. In contrast to other studies, we found that dense volumetric scans with available SDOCT can be used to recover corneal biometric values—including power—that correspond well with existing clinical measurements.

Measurement of corneal biometric values is a requisite for surgical alteration of ocular optical elements. Procedures such as cataract surgery—among the most common surgical procedures performed worldwide—are dependent on the ability to characterize the optical properties of the eye. In particular, measurement of the optical power of the cornea—representing two-thirds of the refractive power of the eye—is critical in achieving desired refractive results.

For the purposes of current clinical ophthalmic optics as is used in cataract surgery, the cornea is assumed to be a spherical refractive element, and the curvature of this element is then related to its refractive power via lens formulas [1,2]. Illuminated ring based platforms such as keratometry and topography have been in routine use for the measurement of corneal curvature and power in preparation for surgical procedures. However, keratometry and topography are only able to measure the curvature of the anterior corneal surface. To characterize the optical properties of the cornea as a whole, assumptions must be made regarding the curvature and power contribution from the unmeasured posterior corneal surface [3].

These assumptions are valid for routine use in *normal* eyes. However, when the cornea is surgically altered as in laser refractive surgery (LASIK, PRK, and others), these assumptions no longer hold true and the biometric values normally generated by these devices can lead to undesired, erroneous clinical outcomes [4]. There is a need then, to develop tomographic imaging technologies that can measure both the anterior and posterior surface curvatures without a fixed, assumed relationship between the two.

Optical coherence tomography (OCT) is a micrometer scale tomographic imaging modality that is in widespread use in ophthalmology [5–10] and could be used for measuring these corneal biometric values. Prior work with time-domain OCT implementations to determine corneal biometry showed promise but were thought to be compromised by motion errors due to the relatively slow sequential data acquisition rates with time-domain OCT [11].

Recent experiences with higher-speed, higher-resolution Fourier domain OCT implementations have been more successful but still noted a discrepancy of over a diopter between corneal power from OCT and existing clinical modalities [12]. Moreover, to minimize the acquisition time, reported spectral domain OCT (SDOCT) efforts have sampled the cornea less densely than found in other tomographic systems and have processed the acquired data primarily in two dimensional planes [12]; reported higher-speed swept-source OCT (SSOCT) efforts have required the use of dedicated research systems specialized for this particular application [13,14].

In this study, we describe the use of existing commercial SDOCT as is widely available in the ophthalmic community to densely sample the cornea volumetrically and to generate common corneal biometric values from the three-dimensional data sets. Furthermore, to ensure a clinically accurate reference for comparison, we chose to study a normal population where the keratometric assumptions made by standard clinical devices about the cornea should remain valid. This eliminated pathology or surgical alterations as confounding variables and allowed us to directly determine the agreement of our SDOCT corneal biometric measurements with existing clinical modalities.

All OCT imaging in this study used a clinical retinal spectral domain OCT system (λ_{0} = 859 nm; Δλ = 53.9 nm; 6 µm axial optical resolution in air, 4.4 µm in tissue; 20 kHz A scan acquisition rate) configured for anterior segment imaging by using a telecentric focusing objective available from the manufacturer (Bioptigen, Research Triangle Park, NC).

Figure 1 briefly illustrates the sample imaging and post-processing procedure used. The scan protocol used volumes composed of 50 radial B-scans each with 1000 A-scans and 1024 pixel depth; at a 20 kHz rate, each volume was acquired in approximately 2.5 seconds. Each B-scan was nominally 6 mm in length. Radial scans were used to maximize the signal to noise ratio in the central region of each B-scan given the curvature of the cornea and the known phenomena of depth-dependent drop-off in SDOCT. Using 50 radial scans was comparable in scan density to that used in other clinical imaging devices such as the Pentacam (Oculus; Wetzler, Germany). Each scan was also centered on the saturation artifact. This artifact is the result of reflection from the apex of a curved surface, and this alignment technique allows for the placement of that apex at the center of the volume. The lateral and axial dimensions and telecentricity of the imaged sample space were characterized by imaging a flat dual-axis linear-scale stage micrometer (NT58-763; Edmund Optics; Barrington, NJ).

Fully automated segmentation of the anterior and posterior corneal surfaces from the images was accomplished via dynamic programming based on graph theory [15]. Rudimentary axial motion correction was made by assuming that the scan axis of rotation was shared in all B-scans, and thus by registering the epithelial surface in the center A-scan of all B-scans within a volume. Removal of optical artifacts such as non-telecentricity and refraction in three-dimensions was accomplished using our previously published algorithms [16] (also described by Ortiz et al. [17]). Refractive indices of samples for three-dimensional refraction correction were provided by the manufacturer for the contact lens phantoms or determined experimentally for the cornea using previously reported techniques [18] which resulted in *n _{cornea}* = 1.387 for our SDOCT system source.

Anterior and posterior corneal curvature values were determined by least squares fitting of the optically-corrected three-dimensional corneal surfaces to the equation for a conicoid of revolution for a sphere [19,20]:

(1)

Here *c* is the inverse of the radius of curvature, (*x, y, z*) are the surface points, and (*x _{0}, y_{0}, z_{0}*) is the vertex. Surface points within the central 5 mm in diameter zone were used for curvature fitting to exclude peripheral areas of low SNR due to SDOCT fall-off.

The paraxial corneal power (inverse front focal length) was then determined via the thick lens equation [1]:

(2)

Here, CCT is the central cornea thickness, the radii are the inverse curvatures from above, and the refractive indices are the Gullstrand values routinely found in the literature with *n _{cornea}* = 1.376,

Because topography devices use a keratometric index (usually given as 1.3375), the rear focal point is actually referenced in these devices to the posterior vertex rather than the rear principal plane as in the paraxial lens formulas [22]. Hence, to also determine a “keratometric equivalent power” for SDOCT, we used the vertex power formula [23] with the appropriate ophthalmic variables:

(3)

Additional detail regarding the scan protocol and post-processing algorithms can be found in Zhao et al. [16]. All post-processing algorithms were implemented and executed in MATLAB (MathWorks; Natick, MA). All statistical analyses were performed in MATLAB and SAS (Cary, NC).

To ensure that the scanning protocol and post-processing algorithms produced valid curvature values, imaging of reference samples was undertaken. Two types of phantoms were used. First, three calibration spheres of varying curvature used for the calibration of a topography device were imaged. This represented the ability to image a curved surface in air such as in the case of the corneal epithelium.

Secondly, 6 rigid gas permeable lenses of varying known base curvatures (conformAthin; Conforma; Norfolk, VA) were each imaged in triplicate. This evaluated the ability to image a posterior curved surface after it had been refracted through an anterior curved surface and passed through some thickness of sample, as would be the case for the corneal endothelial surface. Each of the 6 contacts was placed convex (anterior) side towards the SDOCT onto a rubber grommet on a three-axis linear translation stage and each imaged once. This was then repeated for the group twice more. Each contact lens and the grommet were removed from the stage after each individual measurement. This resulted in volumetric data for 6 contact lenses, each imaged in triplicate, but with some random positional variability (triplicate measurements were not made by simply imaging the contact lens in the same position three times).

The nonparametric Wilcoxon signed rank test was performed to identify any significant differences from zero (with α = 0.05) between the mean SDOCT measures and reference values.

This portion of the study followed the tenets of the Declaration of Helsinki. Informed consent was obtained from the subjects after explanation of the nature and possible consequences of the study. This research was approved by the Duke University Medical Center Institutional Review Board.

Healthy volunteers drawn from researchers and employees at our institution without known ocular pathology other than ametropia were asked to participate in subject imaging. Soft contact lens wearers were asked not to wear their contacts for at least 3 days prior to imaging; no subject wore rigid gas permeable lenses. Each subject was asked to fixate on a fixation target aligned with the optical axis of the SDOCT system, and the above described scan protocol was used to acquire three volumetric data sets of the subject’s cornea. During the same session, each SDOCT imaged eye was also imaged with a topography device (Atlas 995; Carl Zeiss Meditec; Dublin, CA) and a Scheimpflug imaging device (Pentacam) for comparison. The SDOCT image data was post-processed as described above, and the anterior corneal curvature (r_{a}), posterior corneal curvature (r_{p}), corneal power (Φ, Φ_{KEP}), and central corneal thickness (CCT) for each imaged cornea were calculated. Comparable values from the topography device (r_{a} at (0,0) and mean simulated keratometry—SimK) and the Scheimpflug device (mean r_{a}, mean r_{p}, Equivalent K Reading—EKR, and CCT) were generated by each respective device’s own proprietary, on-board software and used directly.

The mean of each group of triplicate measures was used to compare analogous values from each device in a pair-wise fashion via Bland-Altman plots [24]. Paired t-tests by generalized estimating equations to account for multiple imaged eyes per person were used to identify any significant differences from zero (with α = 0.05) between the SDOCT measures and reference values. Agreement between devices was determined by calculating pair-wise intraclass correlation coefficients (ICC). A bootstrapping type of resampling method was used to determine ICC to account for multiple imaged eyes from the same subject [25]. The standard deviation of the triplicate measures on each device was used to indicate the variability of intra-device measurements.

The 3 topography calibration spheres had manufacturer listed radii of curvature of 6.15 mm, 8.00 mm, and 9.65 mm. The measured SDOCT values were 6.19 mm, 8.02 mm, and 9.69 mm. The mean of the paired differences in radii was 0.032 mm with a p-value by Wilcoxon signed rank testing of 0.25.

The 6 rigid gas permeable contact lenses had manufacturer specified base curves of 7.30 mm, 7.50 mm, 7.60 mm, 7.70 mm, 7.80 mm, and 8.20 mm. The manufacturer tolerance for the base curves was ±0.02 mm. Table 1 shows the measured SDOCT values for each contact lens. The mean of the paired differences was 0.003 mm with a p-value by Wilcoxon signed rank test of 1.00.

Each contact lens also had a manufacturer specified central thickness of 130 µm ± 20 µm each. The mean measured SDOCT central thicknesses for each of the 6 contact lenses was 133.67 µm (±0.12 µm), 136.10 µm (±0.10 µm), 125.23 µm (±0.23 µm), 131.33 µm (±0.12 µm), 143.77 µm (±0.15 µm), and 140.83 µm (±0.15 µm).

29 eyes from 15 healthy subjects (mean age 30.7 years old, range 22 to 47 years old) were imaged. 9 of the subjects wore refractive correction with a mean spherical equivalent spectacle correction of −5.65 D ± 3.48 D in that group of 9.

Three volumes from 3 eyes of 3 subjects were unable to be segmented by the automatic segmentation algorithm. These were discarded, so for those 3 eyes, there were only duplicate SDOCT measurements and not triplicate measurements as in the other 26 eyes included in this study.

Data for corneal anterior radius measurements and corresponding Bland-Altman plots are shown in Fig. 2 . The mean paired difference between SDOCT and topography (SDOCT-topography) was 0.016 mm ± 0.101 mm. This was not significantly different by paired t-testing with generalized estimating equations (p = 0.5025). The ICC by resampling method was 0.94 with lower and upper confidence limits of 0.88 to 1.00. For SDOCT and Scheimpflug photography (SDOCT-Scheimpflug photography), the mean paired difference in corneal anterior radius was −0.027 mm ± 0.075 mm. This was not significantly different by paired t-testing with generalized estimating equations (p = 0.1556). The ICC was 0.96 with lower and upper confidence limits of 0.91 to 1.00. For Scheimpflug photography and topography (Scheimpflug photography-topography), the mean paired difference was 0.043 mm ± 0.048 mm. The ICC was 0.98 with lower and upper confidence limits of 0.95 to 1.00.

The mean paired difference between SDOCT paraxial corneal power (Φ) and topography SimK (SDOCT-topography) was −0.91 D ± 0.52 D. This was significantly different by paired t-testing with generalized estimating equations (p < 0.0001). The ICC by resampling method was 0.81 with lower and upper confidence limits of 0.64 to 0.98. For SDOCT and Scheimpflug photography EKR (SDOCT-Scheimpflug photography), the mean paired difference in corneal power was −0.71 D ± 0.52 D. This was significantly different by paired t-testing with generalized estimating equations (p < 0.0001). The ICC was 0.85 with lower and upper confidence limits of 0.71 to 1.00.

Figure 3 shows the data and corresponding Bland-Altman plots when SDOCT keratometric equivalent power (as defined by Eq. (3) in the Methods) is used for comparison. The mean paired difference between SDOCT keratometric equivalent power (ΦKEP) and topography SimK (SDOCT-topography) was −0.10 D ± 0.53 D. This was not significantly different by paired t-testing with generalized estimating equations (p = 0.43). The ICC by resampling method was 0.95 with lower and upper confidence limits of 0.88 to 1.00. For SDOCT and Scheimpflug photography EKR (SDOCT-Scheimpflug photography), the mean paired difference was 0.09 D ± 0.54 D. This was not significantly different by paired t-testing with generalized estimating equations (p = 0.48). The ICC was 0.94 with lower and upper confidence limits of 0.88 to 1.00. For Scheimpflug photography EKR and topography SimK (topography-Scheimpflug photography), the mean paired difference was 0.19 D ± 0.29 D. The ICC was 0.98 with lower and upper confidence limits of 0.95 to 1.00.

Topography is unable to measure posterior corneal radii or central corneal thickness, so for those two biometric measurements, only a comparison between SDOCT and Scheimpflug photography was made. The mean paired difference between SDOCT and Scheimpflug photography measurements of posterior corneal radii (Scheimpflug photography—SDOCT) was 0.180 mm ± 0.076 mm; this was significantly different by paired t-testing with generalized estimating equations (p < 0.0001). The mean standard deviation of repeated posterior corneal radii SDOCT measurements was 0.028 mm (range 0.003 mm to 0.113 mm). The mean standard deviation of repeated posterior corneal radii measurements with Scheimpflug photography was 0.040 mm (range 0.006 mm to 0.121 mm). The ICC by resampling method was 0.74 with lower and upper confidence limits of 0.50 to 0.99.

For central corneal thickness, the mean difference between SDOCT and Scheimpflug measurements (Scheimpflug photography-SDOCT) was 21.27 µm ± 8.72 µm; this was significantly different by paired t-testing with generalized estimating equations (p < 0.0001). The mean standard deviation of repeated central corneal thickness measurements with SDOCT was 1.99 µm (range 0.148 µm to 7.934 µm) and with Scheimpflug photography was 4.21 µm (range 0 µm to 19.655 µm). The ICC by resampling method was 0.71 with lower and upper confidence limits of 0.53 to 0.88.

Figure 4 summarizes the standard deviation of repeated measures to demonstrate the measurement variability for keratometric power and corneal anterior radius, and Fig. 5 summarizes the relationships between the studied imaging devices for the same measures.

In this study, we have described a process to acquire relatively dense corneal tomographic volumes with a clinically available SDOCT system and to generate clinically useful corneal biometric measures. We then tested this process on phantom samples (calibration spheres and rigid gas permeable contacts lenses) and found that the SDOCT derived measurements did not differ significantly from the expected reference values. This demonstrated the validity of the process under controlled conditions.

We proceeded to apply this process to the clinical setting in normal eyes which allowed for the comparison of the SDOCT derived measures to those from existing clinical devices; using normal eyes allows the existing clinical devices to serve as reliable “gold standards” for comparison. Here we found that SDOCT measures of corneal anterior radius and keratometric equivalent power were in good agreement with topography and Scheimpflug photography. However, SDOCT derived paraxial corneal power was significantly different from topographic SimK values and Scheimpflug photography EKR values. Additionally, there was not good agreement between Scheimpflug photography derived corneal posterior radius and central thickness values using our process.

Addressing first the differences in paraxial corneal power, topography uses an artificial keratometric refractive index (typically *n _{k}* = 1.3375) to convert the only measured value—anterior corneal curvature—to a “power” value via P = (

The second assumption when *n _{k}* is used is that the posterior corneal curvature is related to the anterior curvature via a fixed ratio (usually r

The Scheimpflug photography r_{p}:r_{a} in our study population was 0.82 ± 0.010. This is similar to other studies utilizing the Pentacam which found the r_{p}:r_{a} ratio in normal eyes to be 0.82 ± 0.02 [27,28]. Utilizing this ratio, the expected effect on corneal power from Gullstrand model eye values is approximately 0.45 D. This effect, however, was not seen when comparing Pentacam EKR to topography SimK or SDOCT keratometric equivalent power (Fig. 5). This is likely because the algorithm used to generate EKR reportedly utilizes *n _{k}* within its calculation and effectively benchmarks itself to the keratometric power, negating the expected theoretical change [28].

Because the measured r_{a} values are similar in Scheimpflug photography and SDOCT in this study, the source of the r_{p}:r_{a} difference is in the significant difference between measured r_{p}. Our phantom data suggests that our SDOCT processing accurately recovers posterior curvature values with little variability, but this was under ideal conditions and not the clinical setting. If we assume that all devices can measure r_{a} similarly, we can use clinical r_{p}:r_{a} ratios in the literature to approximate the comparison of r_{p} values. In the literature, clinical r_{p}:r_{a} values range from 0.81 to 0.82 (Scheimpflug photography, Purkinje imagery) [29,30] to 0.84-0.85 (slitlamp photography, photokeratoscopy and pachymetry) [31,32]. In another study using SDOCT, the r_{p}:r_{a} ratio was found to be 0.84 [12]. Our ratio of 0.85 is within this range but on the higher end; the differences seen in these ratios are likely dependent on the techniques and methods used to remove optical artifacts from the imaged surfaces.

In addition to the posterior radii difference, SDOCT by our process also produces a central corneal thickness that is significantly different from Scheimpflug photography. Other comparisons of Scheimpflug photography and OCT measures of corneal thickness have shown variable outcomes with OCT lower in some [33,34] as in our study but equal in others [35,36]. In stationary phantom test objects with fixed indices, our process did recover the expected reference values with little variability in repeated measures for central thickness. We were unable to find the specified optical or pixel resolution of the Pentacam, but grossly viewing the output images, the Pentacam appears to have a lower optical resolution, both axial and transverse, than that of SDOCT. Clinically, our variability in repeated measures for the same biometric measures using SDOCT was also lower than that for Scheimpflug photography. There are likely additional differences between the imaging platforms in post-processing that may have contributed to the observed differences.

Motion errors are inevitable with any sequentially scanning imaging platform. While we performed rudimentary axial motion correction in this study, we did not address lateral motion. In previous work, we found that corneal power measurement was less sensitive to lateral motion than axial motion [16], but fully compensating for both axial and lateral motion would likely improve the accuracy of the imaged data and downstream measurements.

Faster scanning to reduce total scan time would also reduce potential corruption by motion. This was seen in the transition from slower TDOCT to faster SDOCT using 8 B-scan acquisition patterns. While the 8 B-scan pattern is acquired in only hundreds of milliseconds, the linear distance between adjacent 6 mm B-scans is up to 10% of the corneal diameter (1.17 mm) at the periphery of the scan. By using 50 6 mm B-scans in our study, the maximum linear distance between scans drops by an order of magnitude to 0.189 mm; this theoretically should produce a more comprehensive representation of the cornea. Despite our increased scan time, we were still able to recover anterior corneal curvatures that corresponded with existing clinical measures. In addition, if we express repeatability as pooled standard deviations to allow for direct comparison, our dense 50 B-scan volumes had a repeatability of 0.14 D which is comparable to that of the faster, less densely sampled corneal SDOCT patterns in a previous study (0.19 D) [12].

By utilizing a dense scanning protocol, our OCT derived surfaces could also potentially be used directly in non-paraxial applications such as ray tracing. However, as our quantitative results only examined spherically fitted radii and derived power as is used in clinical biometry, we cannot yet comment on the accuracy of higher frequency spatial information in the extracted surfaces. Recent work by Ortiz et al. examining the surface elevations compared to reference modalities in a small series showed qualitatively comparable results, though there were differences in aspheric descriptors [37]. Should the higher frequency information be found to be more sensitive to motion artifacts, next-generation research SSOCT systems could mitigate motion artifacts by increasing imaging speeds while still retaining dense scan patterns [14,38]. Also, despite the good repeatability and correlation with SDOCT, the standard deviations of the differences between SDOCT and the other devices are high. This variability may be due to unresolved motion errors which could be reduced with increased imaging speeds.

We did not account for discrepancies between imaging system axis, ocular “optical” axis, and visual axis [21] other than asking subjects to fixate on a fixation spot coaxial with the system optical axis. Due to the limited imaging depth field of view in current-generation commercial SDOCT systems, we were unable to simultaneously image other ocular structures (iris, pupil) that would otherwise be helpful to locate those axes. Introducing a second camera, customizing the spectrometer for extended scan depth at the expense of resolution [39], or implementing complex conjugate artifact removal [40–43] are alternative customized strategies for imaging the deeper structures required for axis determination. Regardless, the spherical radii of curvature currently used clinically and in this study are only low pass filtered representations of the surfaces. We estimated the effect of an average disparity in axes [44] with spherical fitting would alter our power values by only 0.07 D [16].

We have demonstrated the accurate recovery of curvature values in phantom objects with dense, volumetric SDOCT scans. Clinically, the same acquisition process was then applied to normal corneas which showed good agreement of SDOCT measured anterior corneal radius and keratometric equivalent corneal power with existing clinical corneal biometric devices. The process described in this work for recovery of corneal biometric values from SDOCT for clinical use is broadly applicable to any currently available clinical SDOCT system with adequate characterization of the sample space.

This work was supported by grants from the NIH (K12EY01633305, R21EY020001, K23EY021522) and the Duke-Coulter Translational Partnership.

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