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Nucleic Acids Res. Jun 24, 1988; 16(12): 5305–5322.
PMCID: PMC336769
Evolution and mutagenesis of the mammalian excision repair gene ERCC-1.
M van Duin, J van den Tol, P Warmerdam, H Odijk, D Meijer, A Westerveld, D Bootsma, and J H Hoeijmakers
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Abstract
The human DNA excision repair protein ERCC-1 exhibits homology to the yeast RAD10 repair protein and its longer C-terminus displays similarity to parts of the E. coli repair proteins uvrA and uvrC. To study the evolution of this 'mosaic' ERCC-1 gene we have isolated the mouse homologue. Mouse ERCC-1 harbors the same pattern of homology with RAD10 and has a comparable C-terminal extension as its human equivalent. Mutation studies show that the strongly conserved C-terminus is essential in contrast to the less conserved N-terminus which is even dispensible. The mouse ERCC-1 amino acid sequence is compatible with a previously postulated nuclear location signal and DNA-binding domain. The ERCC-1 promoter harbors a region which is highly conserved in mouse and man. Since the ERCC-1 promoter is devoid of all classical promoter elements this region may be responsible for the low constitutive level of expression in all mouse tissues and stages of embryogenesis examined.
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