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Eur J Heart Fail. Jun 2012; 14(6): 571–580.
Published online Apr 1, 2012. doi:  10.1093/eurjhf/hfs038
PMCID: PMC3359860
Mechanical unloading reverses transverse tubule remodelling and normalizes local Ca2+-induced Ca2+release in a rodent model of heart failure
Michael Ibrahim,1 Manoraj Navaratnarajah,1 Urszula Siedlecka,1 Christopher Rao,1 Priyanthi Dias,1 Alexey V. Moshkov,2 Julia Gorelik,2 Magdi H. Yacoub,1 and Cesare M. Terracciano1*
1Laboratory of Cell Electrophysiology, Harefield Heart Science Centre
2Laboratory of Functional Microscopy, Imperial College London, London, UK
*Corresponding author. Harefield Heart Science Centre, Imperial College London, London UB9 6JH, UK. Tel: +44 1895 453874, Fax: +44 1895 828 900, Email: c.terracciano/at/imperial.ac.uk
Received January 25, 2012; Revisions requested February 28, 2012; Accepted March 2, 2012.
Aims
Ca2+-induced Ca2+ release (CICR) is critical for contraction in cardiomyocytes. The transverse (t)-tubule system guarantees the proximity of the triggers for Ca2+ release [L-type Ca2+ channel, dihydropyridine receptors (DHPRs)] and the sarcoplasmic reticulum Ca2+ release channels [ryanodine receptors (RyRs)]. Transverse tubule disruption occurs early in heart failure (HF). Clinical studies of left ventricular assist devices in HF indicate that mechanical unloading induces reverse remodelling. We hypothesize that unloading of failing hearts normalizes t-tubule structure and improves CICR.
Methods and results
Heart failure was induced in Lewis rats by left coronary artery ligation for 12 weeks; sham-operated animals were used as controls. Failing hearts were mechanically unloaded for 4 weeks by heterotopic abdominal heart transplantation (HF-UN). HF reduced the t-tubule density measured by di-8-ANEPPS staining in isolated left ventricular myocytes, and this was reversed by unloading. The deterioration in the regularity of the t-tubule system in HF was also reversed in HF-UN. Scanning ion conductance microscopy showed the reappearance of normal surface striations in HF-UN. Electron microscopy revealed recovery of normal t-tubule microarchitecture in HF-UN. L-type Ca2+ current density, measured using whole-cell patch clamping, was reduced in HF but unaffected by unloading. The variance of the time-to-peak of the Ca2+ transient, an index of CICR dyssynchrony, was increased in HF and normalized by unloading. The increased Ca2+ spark frequency observed in HF was reduced in HF-UN. These results could be explained by the recoupling of orphaned RyRs in HF, as indicated by immunofluorescence.
Conclusions
Our data show that mechanical unloading of the failing heart reverses the pathological remodelling of the t-tubule system and improves CICR.
Keywords: HF, Left ventricular assist device, Recovery
The transverse (t)-tubules of ventricular cardiomyocytes are an extensive system of membrane invaginations which are critical to Ca2+-induced Ca2+ release (CICR), and therefore to cellular contractility.1,2 The efficiency of CICR is largely determined by the proximity of the Ca2+ release trigger [dihydropyridine receptors (DHPR)] to the sarcoplasmic reticulum (SR) Ca2+ release channels [ryanodine receptors (RyRs)].2 As the DHPRs reside in the t-tubules, this proximity depends on normal t-tubule structure.
The t-tubules are disrupted in mechanical overload3 and heart failure (HF).4 T-tubule disruption is common to HF irrespective of aetiology and species.2,5 T-tubule defects and aberrant Ca2+ handling are spatially co-localized, and may be mechanistically linked.2,3,6,7 T-tubule disruption uncouples the normally tight interaction between DHPRs and RyRs.3 Such changes occur early in the progression from hypertrophy to HF, suggesting that they may be important drivers of dysfunction.8 The t-tubule system appears to be specifically load sensitive,9 as evidenced by changes during mechanical overload8 and unloading.10
Heart failure remains a serious clinical issue with a poor prognosis.11 Left ventricular assist device (LVAD) therapy is used to sustain the circulation of patients in end-stage HF in whom they can induce myocardial recovery.1214 However, clinical trials suggest that mechanical unloading induces initial functional improvements in the native heart which regress over time.15 Prolonged mechanical unloading of normal hearts distorts the t-tubules, resulting in dysfunctional Ca2+ handling.10 However, it is possible that in cardiac tissue subjected to chronic overload, such as in failing hearts, the disruption of the t-tubule system regresses with normalization of the load during mechanical unloading.
To test the hypothesis that t-tubule dysfunction of HF is reversible by mechanical unloading (HF-UN) and that this improves cellular Ca2+ handling, we studied the structural and functional properties of failing rat myocytes 4 weeks after heterotopic abdominal heart transplantation.
Extensive details may be found in the Supplementary material online. Syngeneic male Lewis rats (12 animals in total, 10–12 weeks old, ~220 g) were used in all experiments. All animal procedures were approved by the UK Home Office. Four animals were used in each group.
Heart failure model
Heart failure was defined as an ejection fraction of <40% (measured by echocardiography) 12 weeks after left coronary artery ligation (see Supplementary material online). Sham-operated animals were used as controls.
Mechanical unloading model
After the failing heart was harvested from the thorax, it was heterotopically transplanted into the abdomen of an age-matched syngeneic recipient as described previously16 for 4 weeks.
Cell isolation
Cardiomyocytes were isolated by standard enzymatic digestion only from non-infarcted left ventricular (LV) tissue as described previously.17 All recordings were performed with cells superfused with normal Tyrode's solution (140 mM NaCl, 6 mM KCl, 1 mM MgCl2, 1 mM CaCl2, 10 mM glucose, and 10 mM HEPES, adjusted to pH 7.4 with 2 M NaOH) unless otherwise indicated.
Ca2+ handling imaging and electrophysiology
Fluo-4 AM and confocal microscopy were used to record Ca2+ transients and sparks (diastolic Ca2+ release events). The scale alongside line scans shown in Figure 1 are greyscale charts of the fluorescence range, normalized to the background. Indo-1 was used to assay SR Ca2+ content as previously described.18,19 Cells were field stimulated at 1 Hz to obtain steady-state contractions.
Figure 1
Figure 1
Mechanical unloading (HF-UN) induces a normalization of Ca2+ transient parameters. The time-to-peak, decay to 50%, and decay to 90% of the Ca2+ transient were prolonged in heart failure (HF). Mechanical unloading normalized these parameters. The amplitude (more ...)
L-type Ca2+ current was measured in voltage-clamp mode as described previously.20
Imaging of transverse tubules
Cells were stained with di-8-ANEPPS (Molecular Probes, OR, USA) and imaged using confocal microscopy.10 During the Fourier analysis to assess t-tubule regularity, a central portion of the high resolution image of the cell was analysed and was always of fixed dimensions. To avoid bias, cells were codified and blinded. Imaging of cell surface topography was performed using scanning ion conductance microscopy (SICM). For electron microscopy, isolated cardiomyocytes were attached to coverslips using a Shandon Cytospin 2® centrifuge and then digital micrographs were taken using Gatan digital micrograph software at ×30 000 magnification and analysed by measuring the maximum t-tubule diameter of transverse t-tubules, the number of t-tubules per optical section.
Protein subcellular localization and western blotting
Co-localization of DHPRs and RyRs was determined using immunofluorescence on isolated, fixed (with cold acetone) cardiomyocytes. Mouse anti-DHPR alpha 1 (Abcam, Cambridge, UK) antibodies were used against DHPRs, and rabbit anti-RyR (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) was used against RyRs. The percentage overlap between signals was calculated as an index of co-localization. Standard western blotting was performed to measure the expression of junctophilin-2 (JP-2; junctophilin-2 rabbit polyclonal antibody, LifeSpan Biosciences LS-C82883).
Statistical analysis
Statistical analysis was performed using non-parametric one-way analysis of variance (ANOVA; Kruskall–Wallis test). Dunn's post-hoc test was used to test for differences between groups. The analysis was performed using Prism 4 software (GraphPad software Inc., San Diego, CA, USA). P < 0.05 was taken as significant.
Ca2+ transient features are improved after mechanical unloading
Heart failure cells showed a depressed Ca2+ transient amplitude, which improved in HF-UN. An important determinant of the Ca2+ transient amplitude is SR Ca2+ content, which was depressed in HF and increased towards sham values in HF-UN (ratio units: sham, 0.212 ± 0.07, n = 53; HF, 0.162 ± 0.04, n = 20; HF-UN, 0.238 ± 0.05, n = 47; sham vs. HF, P = 0.01, HF vs. HF-UN, P = 0.001; sham and HF-UN were not statistically different). HF cells showed a prolonged time to peak of the Ca2+ transient as well as time to 50% and 90% decline in the Ca2+ transient. These features were also normalized by HF-UN (Figure 1). The variance of the time-to-peak of the Ca2+ transient measured at each pixel is taken as an index of CICR dyssynchrony and was increased in HF cells, but recovered in HF-UN (Figure 1). This suggests that the stimulus activates Ca2+ release throughout the cell more uniformly in HF-UN. There are a number of possible causes for this normalization of CICR including normalized L-type Ca2+ channel activity or normalized RyR function. Another possibility is that coupling between these trigger and release sites, which partly sets the gain of the positive feedback component of CICR, is altered.
Raised Ca2+ spark frequency is reduced by mechanical unloading
The spontaneous opening of RyR clusters can be characterized by measuring Ca2+ sparks. Ca2+ spark frequency was increased in HF cells compared with sham cells, but normalized after HF-UN (Figure 2). HF cells had a significantly higher Ca2+ spark peak amplitude compared with sham myocytes, but unloading did not decrease Ca2+ spark peak amplitude. Ca2+ spark width and duration were increased by HF and mechanical unloading (Figure 2).
Figure 2
Figure 2
Mechanical unloading (HF-UN) normalizes Ca2+ spark frequency. Heart failure (HF) cells showed a higher Ca2+ spark frequency, peak, width, and duration. Mechanical unloading normalized Ca2+ spark frequency and increased width and duration. Mechanical unloading (more ...)
Mechanical unloading causes a regression of cellular hypertrophy
We assessed the volume of single cardiomyocytes using three-dimensional reconstruction of di-8-ANNEPPS images. Mechanical unloading induced a regression of hypertrophy, and average cell volume was smaller than for sham cells (Figure 3).
Figure 3
Figure 3
Mechanical unloading (HF-UN) caused a regression of cellular hypertrophy. Mechanical unloading caused a regression of the cellular hypertrophy observed in heart failure (HF), to below sham levels. The cell capacitance, a measure of cell area, was increased (more ...)
Depressed L-type Ca2+ channel activity is rescued by mechanical unloading
L-type Ca2+ current activity was depressed in HF and was normalized in HF-UN (Figure 4). Cell capacitance, an index of cell surface area, showed a normalization to sham levels in HF-UN (Figure 4). The rate of activation was unaffected (data not shown), but the rate of fast, Ca2+-dependent inactivation was faster in HF and was normalized in HF-UN (Figure 4).
Figure 4
Figure 4
Mechanical unloading (HF-UN) recovers the depressed L-type Ca2+ channel density observed in heart failure (HF). Raw traces of L-type Ca2+ current are shown. The L-type Ca2+ current density was reduced in HF and normalized by mechanical unloading (sham (more ...)
Mechanical unloading restores normal transverse tubule structure
Heart failure reduced the t-tubule density significantly compared with sham, and this was recovered by HF-UN (Figure 5). The deterioration in the regularity of the t-tubule system in HF, as measured by the power of the dominant frequency of the Fourier transform, was also normalized by HF-UN (Figure 5).
Figure 5
Figure 5
Mechanical unloading (HF-UN) restores the normal transverse tubule (t-tubule) density and regularity. Single ventricular cardiomyocytes stained with di-8-ANEPPS are shown. Heart failure (HF) resulted in a reduced t-tubule density and a lower power of (more ...)
Transverse tubule improvements after mechanical unloading are accompanied by improved cell surface structure
In sham cells, clearly defined z-grooves were present which contain the t-tubule openings. This was characterized by a high z-groove index. The cell surface was flattened and distorted in HF, with a reduction in the z-groove index. These features recovered after HF-UN (Figure 6).
Figure 6
Figure 6
Mechanical unloading (HF-UN) restores normal cell surface architecture. The z-groove index is an index of the regularity of the cell surface. This was reduced in heart failure (HF) and normalized by mechanical unloading (sham n = 14, HF n = 25, HF-UN (more ...)
Mechanical unloading restores normal transverse tubule microarchitecture
Transverse tubule microarchitecture was assessed using transmission electron microscopy of single cardiomyocytes. The t-tubule lumens were reduced in density and dilated in HF. HF-UN normalized these parameters towards sham levels (Figure 7).
Figure 7
Figure 7
Mechanical unloading (HF-UN) restores transverse tubular (t-tubular) microarchitecture. Optical sections of transmission electron micrographs of isolated ventricular cardiomyocytes are shown. Heart failure (HF) cells showed fewer total t-tubules per optical (more ...)
Mechanical unloading restores dihydropyridine receptor–ryanodine receptor coupling
Because the repair of the t-tubule system was associated with functional improvements to the CICR process, we assessed whether structural restoration of the DHPR–RyR relationship might account for the improved cellular Ca2+ handling. In HF, the degree of co-localization of DHPRs and RyRs was reduced, but partially recovered in HF-UN (Figure 8).
Figure 8
Figure 8
Mechanical unloading (HF-UN) recouples orphaned ryanodine receptors (RyRs) from dihydropyridine receptors (DHPRs). Examples of RyR (top panel), DHPR (middle panel), and co-localized pixels (CP, lower panel) are shown. In heart failure (HF), DHPR–RyR (more ...)
Junctophilin-2 is not directly responsible for improved dyadic coupling
Junctophilin-2 has been proposed as a regulator of the t-tubule system which is responsible for coupling t-tubule and SR membranes. Its expression is reduced with the progression from hypertrophy to HF,8 possibly due to increasing overload. To test whether the improvements in t-tubule structure were due to changes in JP-2 expression, we performed western blotting (Supplementary material online, Figure S1). JP-2 expression was significantly reduced in HF, but not recovered by HF-UN.
We report that mechanical unloading of failing hearts results in regression of a number of pathological Ca2+ handling properties. Unloading normalized the t-tubule density and regularity, which resulted inDHPR–RyR recoupling. Unloading also normalized the L-type Ca2+ current density.
Ca2+ handling improvements
Heart failure cardiomyocytes show disrupted Ca2+ release synchronicity.2,3,57,24,25 Our study also shows Ca2+ transients severely disrupted in HF and with multiple points of delayed SR Ca2+ release across the cell compared with sham. Points of delayed SR Ca2+ release are localized to gaps in the t-tubule system.3,6,7 SR Ca2+ release is initiated at t-tubules but propagates more slowly to activate RyRs in regions devoid of t-tubules.2 Our immunofluorescence experiments suggest that the irregular nature of the t-tubule system may account for a considerable element of Ca2+ transient disruption. The reappearance of regular t-tubules probably accounts for the improvements in Ca2+ release synchronicity by improving DHPR–RyR coupling.
We also report SR Ca2+ content depression in HF, a critical determinant of Ca2+ transient amplitude. The SR Ca2+ content improves after mechanical unloading, which may contribute to the improvement in the Ca2+ transient amplitude. Changes to the RyR phosphorylation status and action potential (AP) morphology are features which we did not investigate that may contribute to the changes in the Ca2+ transient. Previous studies suggest that mechanical unloading alone does not influence the AP morphology,10 but whether this is true in the context of the failing heart is not known. Recent computational studies26 suggest that AP changes per se are not primarily responsible for dyssynchronous Ca2+ release in rodent HF, and that other changes, including t-tubule abnormalities, may be more important. In larger species, such as man, AP changes are an important contributor to dyssynchrony.
Ca2+ sparks are due to opening of RyR clusters, and their dynamics are a reflection of the status of these clusters. In HF, spontaneous Ca2+ sparks occur more frequently at gaps in the t-tubules, suggesting that they were generated by uncoupled RyRs.27 This uncoupling is caused by disruptions to the t-tubule system3,28 and could explain our finding that global Ca2+ spark frequency is raised in HF. This may also explain why Ca2+ spark frequency is normalized after the reappearance of t-tubules in HF-UN. The Ca2+ spark amplitude, duration, and width are determined by mechanisms which are incompletely understood, but include the SR Ca2+ content, the functional status of RyRs, the buffering properties of the cells, and possibly the activity of the Na+/Ca2+ exchanger (NCX).2931 All these elements are affected in HF and their interaction sets the Ca2+ spark amplitude (and other Ca2+ spark features) which is increased in our model, despite reduced SR Ca2+ content. Direct effects of phosphorylation on RyR open probability, due to involvement of Ca2+/calmodulin-dependent protein kinase II32 or accessory proteins33 in HF, may occur, but whether these aspects contribute to the Ca2+ spark changes observed here is not known. The increase in the duration of the Ca2+ sparks observed in HF and HF-UN may be due to a number of factors, including the phosphorylation status of RyR clusters and the interaction between RyR clusters and other ion channels including NCXs. Recent work shows that gaps in the t-tubule system may result in displacement of local NCXs away from RyRs,48 which can prolong the Ca2+ spark as NCX is not available to terminate the local increase in [Ca2+]i.
Regression of cellular hypertrophy
Mechanical unloading induces a regression of cellular hypertrophy.10,34 Mechanical unloading of failing hearts does not cause as large a reduction in cell size as for unloading of normal hearts. Although profound reductions in cell volume, atrophy, may induce dysfunction, there is no direct relationship between cell size and CICR efficacy.10,19,35,36
L-type Ca2+ current changes
We have previously reported that L-type Ca2+ current density is reduced in HF,20 possibly due to reduced numbers of L-type Ca2+ channels, following the loss of t-tubule membrane. Other studies do not show a depressed L-type Ca2+ current density in animal models of HF.37,38 L-type Ca2+ current depends on both the number and the activity of L-type Ca2+ channels. Since there is a reduction in the t-tubule density in our model, it is likely that the number of channels is reduced. Unchanged L-type Ca2+ current density in HF models may be due to increased single channel activity (but reduced numbers).3942 We found normalization of L-type Ca2+ channel activity with unloading, which is due either to new t-tubules containing L-type Ca2+ channels or to increased conductance amongst existing channels. In this study, we report a quicker rate of fast inactivation (Ca2+ dependent) of the L-type Ca2+ current in HF. We49 and several other groups have previously reported that fast tau is increased in HF. It is not clear what the mechanism of this surprising result is, whether this is the result of direct alterations of the channel, its relationship with accessory proteins, or its regulation by external factors.
Transverse tubule physiology
Our study confirms deterioration in t-tubule structure in HF models24,43 and in diseased human myocardium.5 We report for the first time recovery of the t-tubule system in post-ischaemic cardiomyopathy by reducing mechanical load. Only one study, using exercise in murine diabetic cardiomyopathy, has previously shown that t-tubule recovery is possible.44 We show that unloading recovers the t-tubule system and normalizes CICR by DHPR–RyR recoupling. Although we cannot claim a causal relationship, this finding strengthens the argument that the t-tubule dysfunction of the failing heart is an important factor in the derangement of local CICR in HF. One limitation of this study is that we did not measure the protein expression of DHPRs and RyRs. Further studies should do so, as this would clarify the mechanism of reduced L-type Ca2+ current. It would also clarify whether replenishment of RyRs as well as reorganization of RyRs is involved in the cellular recovery observed in HF. The z-groove index provides a measure of the regularity of the membrane structure, which may or may not impact on the DHPR–RyR coupling. In all conditions studied so far, changes to the z-groove index are associated with dysfunctional CICR. An important question for future studies is to elucidate the relationship between the z-groove, subcellular structures, and the t-tubule membrane.
Our model of HF is driven by myocardial overload as it involves a reduction in cell number but leaves the remainder of the heart normally perfused. Myocardial overload results in t-tubular disarray and impairment of local CICR.8 Conversely, prolonged mechanical unloading of normal hearts results in a reduction in the regularity of the t-tubule system and also impairs CICR.10 We proposed that the t-tubule system is dependent on the chronic load of the myocardium, with physiological levels of load associated with an ‘optimal’ t-tubule structure which promotes efficient CICR.9 Prolonged overloading or unloading results in changes to the t-tubule system which are detrimental to CICR. Here we show that normalizing the load of a failing heart can induce reverse remodelling of the t-tubule system and normalize many aspects of cellular Ca2+ handling. Whether further unloading would result in detrimental cellular remodelling is uncertain.
Molecular mechanisms underlying the recovery of transverse tubule structure
The mechanisms mediating the load dependence of the t-tubule structure are poorly defined, but a number of molecules may be involved. Such molecules include Telethonin, which has recently been shown to be both stretch sensitive and involved in orderly t-tubule formation.45 The physiological regulators of other proteins, such as BIN1 which is involved in t-tubule biogenesis and the shuttling of L-type Ca2+ channels to the membrane, is not known.46,47 JP-2, a protein thought to promote dyad coupling, is reduced as cardiac hypertrophy develops into HF,8 and may therefore be load sensitive. JP-2 knockdown disrupts t-tubule structure and results in HF.23 In this study, we confirm the finding that JP-2 is depressed in HF. However, we find no increase in JP-2 after mechanical unloading when t-tubule structure is improved. There are a number of possible explanations for this. First, JP-2 may not be required in abundance in a mechanically unloaded heart, where low levels of JP-2 may be sufficient to support the t-tubule network in these small cells. Secondly, there may be other proteins of unknown identity which play a role. Thirdly, the phosphorylation status and/or conformational state of these proteins may be altered in HF and mechanical unloading, which could be responsible for the changes observed. How these molecular pathways interact in health and disease is a central question for future studies.
Non-recovered features
Mechanical unloading did not recover all of the parameters investigated. Although the important feature of Ca2+ spark frequency was normalized, the width and duration of the Ca2+ sparks were deranged further with respect to sham. It is not clear what mechanism drives this, but it may include changes to the phosphorylation status of the RyR clusters which could be altered by unloading. Cell volume regressed profoundly to below sham levels, indicating the large diminution of cell size which accompanies mechanical unloading.
This study reflects many previous reports of mechanical unloading-induced regression of hypertrophy, and for the first time documents changes to the cell membrane structure responsible for effective excitation–contraction coupling as an important player in this process.
Conclusions
In conclusion, we show that mechanical unloading of failing hearts produces recovery of the t-tubule structure with improvement of local CICR, probably as a result of enhanced DHPR–RyR coupling. These changes constitute a possible cellular mechanism for the initial improvements in cardiac function seen after LVAD therapy, although this requires direct testing in future studies. The mechanisms mediating the retubulation observed in this study require further investigation and may highlight potential new therapeutic targets.
Funding
The British Heart Foundation [M.B.-Ph.D. Grant to M.I. (FS/09/025/27468)]. Funding to pay the Open Access publication charges for this article was provided by the British Heart Foundation.
Conflict of interest: none declared.
Supplementary Material
Supplementary Data
Acknowledgements
We thank Dr Patrizia Camelliti, Dr Padmini Sarathchandra, and Dr Pradeep Luther for assistance with imaging.
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