In August 2009, a 61-year-old Caucasian man who lived in Switzerland sought treatment at the emergency department of University Hospital in Zurich, reporting a 10-day history of malaise, temperature as high as 39.5°C, chills, and moderate dyspnea. Six weeks previously, he had undergone coronary artery bypass graft surgery and mitral valve reconstruction for which prosthetic material was used. The patient had not noticed tick bites or a skin rash; neither did he recall a rodent bite. A pet dog and cat lived in his household.
Physical examination showed a reduced general health condition and a temperature of 38.5°C. Blood pressure was 109/68 mm Hg, heart rate was 86 beats/min, and oxygen saturation was 95% with 2 L nasal oxygen. No murmur was detected on cardiac auscultation. No skin or joint abnormalities were found. Laboratory tests showed elevated leukocytes (12.9 × 103 cells/µL), with a high fraction of neutrophils (10.1 × 103 cells/µL) and thrombocyte count within reference range (277 × 103 cells/µL); aminotransferase levels within reference ranges (aspartate aminotransferase 18 U/L, alanine aminotransferase 20 U/L); and an elevated C-reactive protein (CRP) of 68 mg/L (reference range <5 mg/L). Chest radiograph showed no signs of cardiac decompensation or of pulmonary infiltrates. Transthoracic echocardiograph showed only minor insufficiency of the aortic and tricuspid valves. In addition, degenerative alterations of aortic valve, but no vegetations, were noted with comparable findings in the follow-up echocardiograph 1 week later.
At the follow-up visit, no hints of infectious foci were found. Five sets of blood cultures were drawn with >12 h difference between the first and the last set. Antimicrobial drug treatment for endocarditis with prosthetic material, consisting of vancomycin, gentamicin, and rifampin, was initiated.
Blood cultures remained negative for microbial growth, even after extended incubation. Serologic tests for agents of culture-negative endocarditis and tick-borne diseases were performed. Enzyme immunoassays (EIAs) were positive for immunoglobulin (Ig) G antibodies reactive to Bartonella henselae (512) and B. quintana (1,024), Coxiella burnetii (phase II IgG titer 160), Rickettsia rickettsii/conorii (IgG 256), and Rickettsia typhi (IgG 128), Mycoplasma pneumoniae (index 2.7). IgM was positive only for A. phagocytophilum (512, atypical fluorescence pattern), presenting a low titer of IgG at this stage. Serologic test results for Brucella spp., Chlamydia trachomatis, Chlamydia pneumoniae, and Borrelia burgdorferi were negative. Species-specific PCRs for A. phagocytophilum, Tropheryma whipplei, B. henselae, B. quintana, Legionella spp., and L. pneumophila were negative.
Bacterial broad-spectrum 16S rRNA gene PCR, followed by sequence analysis, identified Candidatus
Neoehrlichia mikurensis in 4 of 8 sequential blood samples; the 4 samples that tested positive were collected before (day 0) and during the initial phase (days 7 and 13) of an effective course of antimicrobial drug therapy (). For 16S rRNA gene amplification, DNA was extracted and amplified from anticoagulated blood (4-mL EDTA tubes), uncoagulated blood from a BacT/ALERT SA aerobic blood culture flask (bioMérieux SA, Geneva, Switzerland), and coagulated blood (Technical Appendix
) as described (4
). Sequences derived (GenBank accession nos. GQ501089-GQ501091) were analyzed by SmartGene IDNS software (Zug, Switzerland). We amplified and sequenced (1,150 bp; GenBank accession no. HM045824) an alternative target gene (groEL
) with primers (Technical Appendix
) derived from published sequences (1
), which showed 98% homology to previously published Candidatus
Neoehrlichia mikurensis groEL
sequences (, panel B).
Figure 1 Polyacrylamide gel electrophoresis (SDS-PAGE) analysis of broad range 16S rRNA gene PCR products obtained from blood samples. Lane 1, marker, 100 bp DNA ladder (Roche DNA Marker XIV); lane 2, positive control, Escherichia coli; following lanes, PCR products (more ...)
Figure 2 A) Phylogenetic tree based on the 16S rRNA gene sequences of Candidatus Neoehrlichia mikurensis GQ501090.2 (our patient’s isolate, herein termed Zurich and indicated in boldface) and related organisms. The number at nodes indicates percentages (more ...)
After being treated with vancomycin, gentamicin, and rifampin for 10 days, the patient became afebrile, and clinical symptoms improved. Leukocyte count was within reference range, and CRP dropped from 68 mg/L to 23 mg/L. At this time, Candidatus Neoehrlichia mikurensis was detected in the first blood sample. Rifampin (450 mg 2×/d) was continued, and vancomycin and gentamicin were switched to oral doxycycline (100 mg 2×/d). Three weeks later, CRP was 1 mg/L, body temperature was within the normal range, and treatment was continued to finish a 6-week course. Two weeks after the end of treatment, the patient was seen for a follow-up visit. Neither clinical nor laboratory results raised any concern of relapse. Results of broad-range PCR of the 16S rRNA gene to detect Candidatus Neoehrlichia mikurensis were negative for the first time in 5 weeks since treatment began initiation and remained negative at the follow-up visit 2 weeks after the end of treatment.
Neoehrlichia mikurensis was previously found in Rattus norvegicus
rats and Ixodes ovatus
ticks in Japan (1
), in R. norvegicus
rats in China (5
), and in I. ricinus
ticks in the Netherlands (6
), Slovakia (8
), and the Asian part of Russia (9
). Closely related rickettsial bacteria () have been identified in Procyon lotor
raccoons in the Piedmont region of Georgia, USA (10
). Another closely related species (Candidatus
Ehrlichia walkeri) has been detected in I. ricinus
ticks collected from humans in northern Italy (11
). The geographic distribution of the tick population has also been studied (12
Our patient lives in a high-risk area for ticks in Switzerland. I. ricinus
is the main tick species in this region. A tick-borne disease appears epidemiologically possible in this patient, who is a golfer and the owner of a large garden and thus is repeatedly exposed to the habitat of the potential vector, I. ricinus
, even though he remembered no tick bites. Of note, only 50%–70% of patients with Lyme disease remember receiving a tick bite (13
). Blood of the patient’s pet animals (dog and cat) was examined by broad-range 16S rRNA gene PCR to exclude presence of bacterial pathogens.
In Wister rats, Candidatus
Neoehrlichia mikurensis has been shown to infect spleen sinus endothelial cells, forming intracellular inclusions on the side of the endosome (by electron microscopy 60 days after infection) (1
). Accordingly, we assumed that in human hosts, valvular endothelial cells are likely involved. The initial antimicrobial drug therapy, which contained rifampin, may already have contributed to the reduction of the bacterial load but was not completely effective (). Thus, following the recommended guidelines for treatment of intracellular rickettsial bacteria with endocardial involvement, we changed to a 6-week course of treatment consisting of rifampin combined with doxycycline (14
). After the end of the course, we observed a successful response.
We detected Candidatus Neoehrlichia mikurensis in 4 of 8 consecutive blood specimens, and repeated analysis showed the disappearance of the pathogen’s DNA during the course of treatment (). Laboratory diagnosis of ehrlichiosis is severely hampered because the relevant pathogens cannot be cultured on routine media. Serologic tests depends on samples collected during acute phase of illness, obtaining comparative samples in the course of the disease, and demonstrating a >4-fold increase in antibody titers.