Toxic liver injury is accompanied by early changes in intestinal permeability and bacterial translocation
Mice were injected intraperitoneally with oil or carbon tetrachloride (CCl4) for indicated times. (A) 24 hrs following a single injection of oil or CCl4, mice were gavaged with 200μl of FITC labeled dextran (100mg/ml), and fluorescence in the plasma was determined 4 hrs later (n=3–5). (B) Plasma endotoxin levels were measured (n=4–7). (C) Colony forming units (CFUs) were counted on aerobic cultures of mesenteric lymph nodes (left panel) (n=4–9) and blood (right panel) (n=14–20); data are presented as median. (D) Occludin protein expression was analyzed in the intestine by Western blotting. β-actin was used as a loading control. Images are representative of one Western blot. Densitometry of Western blot images was performed. Values are presented relative to oil injected mice (n=5 in each group). (E) Immunofluorescent staining for occludin (green) was performed on colonic sections from mice that received one injection of oil or CCl4 one day prior. Nuclei are stained with Hoechst (blue). *p<0.05.