PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of bmccancBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Cancer
 
BMC Cancer. 2012; 12: 97.
Published online Mar 21, 2012. doi:  10.1186/1471-2407-12-97
PMCID: PMC3353225
PRAF3 induces apoptosis and inhibits migration and invasion in human esophageal squamous cell carcinoma
Guo-Zhen Shi,#2 Yang Yuan,#1 Guo-Jun Jiang,#2 Zhi-Jun Ge,3 Jian Zhou,3 De-Jun Gong,1 Jing Tao,1 Yong-Fei Tan,corresponding author2,5 and Sheng-Dong Huangcorresponding author1,4
1Institute of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, PR China
2Department of Cardiothoracic Surgery, Yixing People's Hospital, Jiangsu, PR, China
3Department of Pathology, Yixing People's Hospital, Jiangsu, PR China
4Institute of Cardiothoracic Surgery, Changhai Hospital, 168, Changhai Rd, Shanghai, PR China
5Institute of Cardiothoracic Surgery, Yixing People's Hospital, 75 Tongzhenguan Rd, Wuxi, Jiangsu, PR China
corresponding authorCorresponding author.
#Contributed equally.
Guo-Zhen Shi: staff934/at/yxph.com; Yang Yuan: iamyuanyang/at/gmail.com; Guo-Jun Jiang: dr.jgjun/at/gmail.com; Zhi-Jun Ge: dr.gezhijun/at/gmail.com; Jian Zhou: dr_zhoujian/at/163.com; De-Jun Gong: xxwkdaily/at/gmail.com; Jing Tao: taojing1997/at/163.com; Yong-Fei Tan: tan.yongf/at/gmail.com; Sheng-Dong Huang: huangsd/at/smmu.edu.cn
Received November 29, 2011; Accepted March 21, 2012.
Abstract
Background
Prenylated Rab acceptor 1 domain family member 3 (PRAF3) is involved in the regulation of many cellular processes including apoptosis, migration and invasion. This study was conducted to investigate the effect of PRAF3 on apoptosis, migration and invasion in human esophageal squamous cell carcinoma (ESCC).
Methods
The expression of PRAF3 mRNA and protein in primary ESCC and the matched normal tissues (57cases) was determined by quantitative RT-PCR and Western blot. Immunohistochemical analysis of PRAF3 expression was carried out in paraffin-embedded sections of ESCC and correlated with clinical features. The role of PRAF3 in apoptosis, migration and invasion was studied in ESCC cell lines of Eca109 and TE-1 through the adenovirus mediated PRAF3 gene transfer. The effect of PRAF3 on apoptosis was analyzed by annexin V-FITC assay. The regulation of PRAF3 on migration was determined by transwell and wounding healing assay, while the cellular invasion was analyzed by matrigel-coated transwell assay.
Results
We found that the expression of PRAF3 was significantly down-regulated in ESCC tissue compared with the matched normal tissue and was correlated with the clinical features of pathological grade, tumor stage and lymph node metastasis. Moreover, overexpression of PRAF3 induced cell apoptosis through both caspase-8 and caspase-9 dependent pathways, and inhibited cell migration and invasion by suppressing the activity of both MMP-2 and MMP-9 in human ESCC cell lines.
Conclusions
Our data suggest that PRAF3 plays an important role in the regulation of tumor progression and metastasis and serves as a tumor suppressor in human ESCC. We propose that PRAF3 might be used as a potential therapeutic agent for human ESCC.
Articles from BMC Cancer are provided here courtesy of
BioMed Central