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Logo of bioinorgchemapplBioinorganic Chemistry and Applications
Bioinorg Chem Appl. 2012; 2012: 698491.
Published online 2012 March 7. doi:  10.1155/2012/698491
PMCID: PMC3352140

Synthesis, Characterization and In Vitro Antibacterial Studies of Organotin(IV) Complexes with 2-Hydroxyacetophenone-2-methylphenylthiosemicarbazone (H2dampt)


Five new organotin(IV) complexes of 2-hydroxyacetophenone-2-methylphenylthiosemicarbazone [H2dampt, (1)] with formula [RSnCln-1(dampt)] (where R = Me, n = 2 (2); R = Bu, n = 2 (3); R = Ph, n = 2 (4); R = Me2, n = 1 (5); R = Ph2, n = 1 (6)) have been synthesized by direct reaction of H2dampt (1) with organotin(IV) chloride(s) in absolute methanol. The ligand (1) and its organotin(IV) complexes (2–6) were characterized by CHN analyses, molar conductivity, UV-Vis, FT-IR, 1H, 13C, and 119Sn NMR spectral studies. H2dampt (1) is newly synthesized and has been structurally characterized by X-ray crystallography. Spectroscopic data suggested that H2dampt (1) is coordinated to the tin(IV) atom through the thiolate-S, azomethine-N, and phenoxide-O atoms; the coordination number of tin is five. The in vitro antibacterial activity has been evaluated against Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli, and Salmonella typhi. The screening results have shown that the organotin(IV) complexes (2–6) have better antibacterial activities and have potential as drugs. Furthermore, it has been shown that diphenyltin(IV) derivative (6) exhibits significantly better activity than the other organotin(IV) derivatives (2–5).

1. Introduction

Thiosemicarbazones and their metal complexes have received considerable attention in chemistry and biology, primarily because of their marked and various biological properties [13]. The pharmacological profiles of 2-formyl, 2-acetyl, and 2-benzoylpyridine thiosemicarbazones have been investigated [4]. Seena and Kurup [5] have synthesized and characterized dioxomolybdenum(IV) complexes with 2-hydroxyacetophenone-N(4)-cyclohexyl and N(4)-phenyl thiosemicarbazone which suggested that the Mo(IV) complex is pentacoordinated [5]. For the past few years, studies of the coordination chemistry of thiosemicarbazone involved complexes with transition metal ions [68]. Organotin(IV) complexes have been the subject of interest for some time because of their biomedical and commercial applications including in vitro and in vivo antitumor activity [9, 10]. Many organotin(IV) complexes have been found to be as effective as or even better than traditional anticancer drugs [1114]. Organotin(IV) chelates with nitrogen, sulfur, and oxygen donor ligands have gained attention during the last few years [15]. The coordination chemistry of tin is extensive with various geometries and coordination numbers known for both inorganic and organometallic complexes [16, 17]. In our previous work, we have reported some new organotin(IV) complexes with heterocyclic-N(4)-cyclohexylthiosemicarbazone ligands [18, 19]. The results revealed that thiosemicarbazones derived from 2-benzoylpyridine and 2-acetylpyrazine and their tin(IV)/organotin(IV) complexes have been characterized by different spectroscopic techniques. From the literature survey, the studies on the organotin(IV) complexes derived from substituted thiosemicarbazone ligands containing ONS-donor atoms are still lacking. To the best of our knowledge, there was no report on the organotin(IV) complexes of the 2-hydroxyacetophenone-2-methylphenylthiosemicarbazone. In this view, we have synthesized a series of organotin(IV) complexes with 2-hydroxyacetophenone-2-methylphenylthiosemicarbazone. These complexes have been characterized by elemental analysis, 1H, 13C, and 119Sn NMR spectroscopy. X-ray crystal structure of 2-hydroxyacetophenone-2-methylphenylthiosemicarbazone (1) is also described. Their biological activity data has also been reported.

2. Experimental

2.1. Materials and Methods

All reagents were purchased from Fluka, Aldrich, and JT Baker. All solvents were purified according to standard procedures [20]. UV-Vis spectra were recorded in CHCl3 solution with a Perkin Elmer Lambda 25 UV-Visible spectrometer. Infrared spectra were recorded on KBr discs using a Perkin Elmer Spectrum GX Fourier-Transform spectrometer in the range 4000–370 cm−1 at room temperature. 1H, 13C, and 119Sn NMR spectra were recorded on a JEOL 500 MHz-NMR spectrometer; chemical shifts were given in ppm relative to SiMe4 and SnMe4 in CDCl3 solvent. CHN analyses were obtained with a Flash EA 1112 series CHN elemental analyzer. Molar conductivity measurements were carried out with Jenway 4510 conductivity meter using DMF solvent mode.

2.2. Synthesis of 2-Hydroxyacetophenone-2-Methylphenylthiosemicarbazone (H2dampt) (1)

The 2-methylphenylisothiocyanate (0.746 g, 5 mmol) and hydrazine hydrate (0.253 g, 5 mmol), each dissolved in 10 mL ethanol, were mixed with constant stirring. The stirring was continued for 30 min and the white product, 2-methylphenylthiosemicarbazide, formed was washed with ethanol and dried in vacuo. A solution of the isolated 2-methylphenylthiosemicarbazide (0.540 g, 3 mmol) in 10 mL methanol was then refluxed with a methanolic solution of 2-hydroxyacetophenone (0.408 g, 3 mmol) for 5 h after adding 1-2 drops of glacial acetic acid (Scheme 1). On cooling the solution to room temperature, light-yellow microcrystals were separated and washed with methanol. The microcrystals were recrystallized from methanol and dried in vacuo over silica gel. Yield: 0.74 g, 78%: M.p.: 178–180°C: UV-Visible (CHCl3) λmax /nm: 226, 318, 359: FT-IR (KBr disc, cm−1) νmax : 3175 (s, OH), 3000 (s, NH), 1583 (m, C=N), 1298 (m, C–O), 943 (m, N–N), 1371, 861 (w, C=S). 1H NMR (CDCl3) δ: 10.82 (s, 1H, OH), 9.02 (s, 1H, N–H), 7.31–7.25 (m, 8H, phenyl ring), 2.56 (s, 3H, N=C–CH3), 2.29 (s, 3H, CH3), 1.19 (s, 1H, SH). 13C NMR (CDCl3) δ: 185.20 (NH–C=S), 165.32 (C=N), 145.30–136.21 (aromatic ring), 10.45 (CH3). Anal. Calc. for C16 H17N3OS: C, 64.21; H, 5.73; N, 14.04%. Found: C, 64.17; H, 5.67; N, 14.01%.

Scheme 1
Synthesis of 2-hydroxyacetophenone-2-methylphenylthiosemicarbazone (H2dampt) ligand (1).

2.3. Synthesis of [MeSnCl(dampt)] (2)

H2dampt (0.299 g, 1.0 mmol) was dissolved in absolute methanol (10 mL) in a Schlenk round bottom flask under a nitrogen atmosphere. Then, a methanolic solution of methyltin(IV) trichloride (0.24 g, 1.0 mmol) was added dropwise. The resulting reaction mixture was refluxed for 4 h (Scheme 2) and cooled to room temperature. The microcrystals were filtered off, washed with a small amount of cold methanol, and dried in vacuo over silica gel. Yield: 0.41 g, 76 %: Mp.: 222–224°C: Molar conductance (DMF) Ω-1 cm2 mol−1: 7.1: UV-Visible (CHCl3) λmax /nm: 262, 328, 367, 384: FT-IR (KBr, cm−1) νmax : 3378 (s, NH), 1595 (m, C=N–N=C), 1268 (m, C–O), 1026 (w, N–N), 1306, 822 (m, C–S), 612 (w, Sn–C), 570 (w, Sn–O), 449 (w, Sn–N). 1H NMR (CDCl3,2J[119Sn, 1H]) δ: 9.08 (s, 1H, N–H), 7.26–6.94 (m, 8H, phenyl ring), 2.95 (s, 3H, N=C–CH3), 2.30 (s, 3H, CH3), 1.09 (s, 3H, Sn–CH3), [74.4 Hz]. 13C NMR (CDCl3) δ: 180.55 (N=C–S), 170.88 (C=N), 144.35–135.60 (aromatic ring), 18.70 (CH3), 12.80 (Sn–CH3). 119Sn NMR (CDCl3) δ: −168.5. Anal. Calc. for C17H18N3SOSnCl: C, 43.76; H, 3.88; N, 9.00%. Found: C, 43.71; H, 3.82; N, 8.95%.

Scheme 2
Reaction scheme for the synthesis of organotin(IV) complexes (2–6).

The other complexes (3–6) were synthesized using a similar procedure to organotin(IV) complex (2) using appropriate organotin(IV) chloride(s) (Scheme 2).

2.4. Synthesis of [BuSnCl(dampt)] (3)

Yield: 0.43 g, 74%: Mp.: 226–228°C: Molar conductance (DMF) Ω-1 cm2 mol−1: 9.1: UV-Visible (CHCl3) λmax /nm: 262, 328, 382, 397: FT-IR (KBr, cm−1) νmax : 3374 (s, NH), 1599 (m, C=N–N=C), 1254 (m, C–O), 1014 (w, N–N), 1299, 835 (m, C–S), 605 (w, Sn–C), 568 (w, Sn–O), 443 (w, Sn–N). 1H NMR (CDCl3) δ: 9.07 (s, 1H, N–H), 7.25–7.97 (m, 8H, phenyl ring), 2.62 (s, 3H, N=C–CH3), 2.30 (s, 3H, CH3), 2.28–2.15 (t, 2H, Sn–CH2–CH2–CH2–CH3), 2.14–1.73 (m, 2H, Sn–CH2–CH2–CH2–CH3), 1.24–1.22 (m, 2H, Sn–CH2–CH2–CH2–CH3), 0.99–0.86 (t, 3H, Sn–CH2–CH2–CH2–CH3). 13C NMR (CDCl3) δ: 178.99 (N=C–S), 168.36 (C=N), 145.20–136.22 (aromatic ring), 32.78, 26.31, 24.18, 20.11 (Sn–CH2–CH2–CH2–CH3), 16.44 (CH3). 119Sn NMR (CDCl3) δ: −149.6. Anal. Calc. for C20H24N3SOSnCl: C, 45.10; H, 4.54; N, 7.88%. Found: C, 45.00; H, 4.51; N, 7.81%.

2.5. Synthesis of [PhSnCl(dampt)] (4)

Yield: 0.48 g, 79%: Mp.: 218–220°C: Molar conductance (DMF) Ω-1 cm2 mol−1: 3.11: UV-Visible (CHCl3) λmax /nm: 263, 335, 381, 410: FT-IR (KBr, cm−1) νmax : 3184 (s, NH), 1598 (m, C=N–N=C), 1240 (m, C–O), 1035 (w, N–N), 1300, 838 (m, C–S), 601 (w, Sn–C), 522 (w, Sn–O), 471 (w, Sn–N). 1H NMR (CDCl3) δ: 9.01 (s, 1H, N–H), 7.24-6.95 (m, 13H, phenyl ring), 2.76 (s, 3H, N=C–CH3), 2.29 (s, 3H, CH3). 13C NMR (CDCl3) δ: 180.12 (N=C–S), 173.97 (C=N), 144.84–136.60 (aromatic ring), 16.88 (CH3). 119Sn NMR (CDCl3) δ: −174.73. Anal. Calc. for C22H20N3SOSnCl: C, 49.98; H, 3.81; N, 7.94%. Found: C, 48.92; H, 3.77; N, 7.90%.

2.6. Synthesis of [Me2Sn(dampt)] (5)

Yield: 0.41 g, 78%: Mp.: 210–212°C: Molar conductance (DMF) Ω−1 cm2 mol−1: 5.2: UV-Visible (CHCl3) λmax /nm: 266, 338, 378, 414: FT-IR (KBr, cm−1) νmax : 3320 (s, NH), 1605 (m, C=N–N=C), 1252 (m, C–O), 1036 (w, N–N), 1300, 832 (m, C–S), 603 (w, Sn–C), 523 (w, Sn–O), 499 (w, Sn–N). 1H NMR (CDCl3,2J[119Sn, 1H]) δ: 9.08 (s, 1H, N–H), 7.26–6.94 (m, 8H, phenyl ring), 2.98 (s, 3H, N=C–CH3), 2.30 (s, 3H, CH3), 0.98 (s, 3H, Sn–CH3), [77.5 Hz]. 13C NMR (CDCl3, [1J (13C–119Sn]) δ: 181.10 (N=C–S), 178.45 (C=N), 145.68–137.20 (aromatic ring), 17.5 (CH3), 14.97 (Sn–CH3) [557 Hz]. 119Sn NMR (CDCl3) δ: −182.45. Anal. Calc. for C18H21N3SOSn: C, 48.54; H, 4.74; N, 9.41%. Found: C, 48.50; H, 4.71 N, 9.38%.

2.7. Synthesis of [Ph2Sn(dampt)] (6)

Yield: 0.48 g, 75%: Mp.: 258–260°C: Molar conductance (DMF) Ω−1 cm2 mol−1: 8.17: UV-Visible (CHCl3) λmax /nm: 268, 327, 373, 402: FT-IR (KBr, cm−1) νmax : 3383 (s, NH), 1592 (m, C=N–N=C), 1265 (m, C–O), 1039 (w, N–N), 1307, 821 (m, C–S), 601 (w, Sn–C), 570 (w, Sn–O), 448 (w, Sn–N). 1H NMR (CDCl3) δ: 9.02 (s, 1H, N–H), 7.30–6.97 (m, 13H, phenyl ring), 2.67 (s, 3H, N=C–CH3), 2.29 (s, 3H, CH3). 13C NMR (CDCl3, [1J (13C–119Sn]) δ: 179.98 (N=C–S), 171.75 (C=N), 142.20–138.21 (aromatic ring), 15.88 (CH3) [546 Hz]. 119Sn NMR (CDCl3) δ: −185.32. Anal. Calc. for C28H25N3SOSn: C, 58.97; H, 4.41; N, 7.36%. Found: C, 58.92; H, 4.38; N, 7.30%.

2.8. Antibacterial Test

The synthesized ligand (1) and its organotin(IV) complexes (2–6) were screened in vitro for their antibacterial activity against Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli, and Salmonella typhi bacterial strains using agar-well diffusion method [21]. Wells (size of well 6 mm in diameter) were dug in the media with the help of a sterile metallic borer with centers at least 24 mm. Eight-hour old bacterial inoculums containing 104–106 colony-forming units (CFU)/mL were spread on the surface of the nutrient agar using a sterile cotton swab. Recommended concentration of the test sample (200 mg/mL in DMSO) was introduced in the respective wells. Other wells supplemented with DMSO and reference drug (doxycycline) served as negative and positive controls, respectively. The plates were incubated immediately at 37°C for 20 h. Activity was determined by measuring the diameter of zones showing complete inhibition (mm). Growth inhibition was calculated with reference to the positive control.

3. Results and Discussion

3.1. Synthesis

2-Hydroxyacetophenone-2-methylphenylthiosemicarbazone (H2dampt) was synthesized by the condensation reaction of 2-hydroxyacetophenone and 2-methylphenylthiosemicarbazide in absolute methanol in 1 : 1 mole ratio. It has two tautomers within the structure, existing as either thione or thiol tautomer (Scheme 1). The present organotin(IV) complexes (2–6) were obtained by direct reaction of organotin(IV) chloride(s) and H2dampt (1) in absolute methanol under N2 atmosphere (Scheme 2). The physical properties and analytical data of H2dampt (1) and its organotin(IV) complexes (2–6) are given in the experimental section. All complexes (2–6) were stable under N2 atmosphere and soluble in CHCl3, CH2Cl2, DMF, DMSO, and MeCN solvents except methanol, ethanol, hexane, pentane, THF, and ether. The molar conductances values of the complexes (2–6) are 9.1–3.1 Ω−1 cm2 mol−1, respectively, indicate that the complexes behave as nonelectrolytes [22].

3.2. UV-Visible Spectra

The UV-Vis spectra of ligand (1) and its organotin(IV) complexes (2–6) were carried out in CHCl3 (1 × 10−4 mol L−1) at room temperature. The free ligand (1) exhibited three absorption bands at 262, 318, and 359 nm assigned to the HOMO/LUMO transition of phenolic group, azomethine, and thiolate function, respectively [23]. After complexation, the UV-Vis spectra of the complexes (2–6) exhibited four absorption bands in the region at 262–268, 327–338, 367–382, and 384–414 nm, respectively. In the electronic spectra of the complexes (2–6), the intraligand transition is shifted to higher wavelength as a result of coordination. In the spectra of organotin(IV) complexes (2–6), one new absorption band appeared at 384–414 nm which is assigned to the ligand→metal charge transfer (LMCT) [24]. The shift of the λmax band from the ligand to the complex is supported by the coordination of ligand (1) to the tin(IV) ion.

3.3. IR Spectra

The IR spectrum of free ligand (1) showed absorption bands at 3175 and 3000 cm−1, which are due to the stretching vibrations of the OH and NH groups, respectively. The absorption bands at 1583, 1298, 943, and 1371, 861 cm−1are due to ν(C=N), ν(C–O), ν(N–N), and ν(C=S), respectively. Several significant changes with respect to the free ligand (1) bands on complexation suggest coordination through phenolic group, azomethine, and sulfur of the thiolic form of the ligand. The strong stretching band at 3375 cm−1 that corresponds to the ν(OH) group in the spectrum of ligand (1) has disappeared in the spectra of complexes (2–6) due to the deprotonation, indicating coordination through the phenolic oxygen to tin(IV) atom. The free ligand (1) showed a band at 1298 cm−1 which is due to ν(C–O). This band is shifted to lower wave numbers at 1240–1268 cm−1 in the complexes (2–6), indicating the coordination of O to the tin(IV) atom [25]. The newly formed ν(C=N–N=C) bond showed medium-to-strong absorption peaks in the range at 1592–1605 cm–1 in the spectra of the complexes (2–6), indicating coordination of azomethine nitrogen to tin(IV) atom [26]. A sharp band at 943 cm−1 is due to ν(N–N) for ligand (1) is shifted to higher frequencies at 1014–1039 cm−1 in the spectra of organotin(IV) complexes ((2–6). The increase in the frequency of this band in the spectra of complexes (2–6) due to an increase in the bond length again confirms coordination via the azomethine nitrogen atom [27]. The bands at 1371 and 861 cm−1 in the free ligand (1) due to ν(C=S) stretching vibrations are shifted to lower frequencies at 1299–1307 cm–1 and 821–838 cm−1 in the spectra of the complexes (2–6), suggesting coordination through the thiolate sulfur with tin(IV) atom [28]. The IR bands observed in the range at 570–522 cm–1 in the spectra of the complexes (2–6) suggest the presence of Sn–O bonding in their structure. The ν(Sn–C) and ν(Sn–N) bands are tentatively assigned to absorptions in the regions 612–601 cm–1 and 443–499 cm–1, respectively. Based on the infrared spectra analyses of ligand (1) and its organotin(IV) complexes (2–6), it was suggested that ligand (1) was coordinated to the tin(IV) core through the phenoxide-O, azomethine-N, and thiolato-S atoms.

3.4. 1H NMR Spectra

1H NMR spectrum of free ligand (1) showed resonance signals at 10.82, 9.02, 7.31–7.25, 2.56, 2.29, and 1.19 ppm are due to OH, NH, phenyl ring protons, N=C–CH3, CH3, and SH, respectively. After complexation, the resonance signal of OH proton was absent in the spectra of the complexes (2–6), indicating deprotonation of the phenolic proton and supported the phenolic oxygen atom was coordinated with tin(IV) atom. The resonance signal of SH is not found in the spectra of complexes (2–6) which suggested the deprotonation of the SH proton and confirming that the ligand coordinated to the tin(IV) in the thiolate form. The azomethine proton (N=C–CH3) signal appears at 2.56 ppm in the free ligand (1) which is shifted to high frequency at 2.98–2.62 ppm in the complexes (2–6), supporting the coordination of azomethine nitrogen to the central tin(IV) atom. The resonance signals for the protons of phenyl moiety of the ligand (1) were observed at 7.31–7.25 ppm, which is shifted to low frequency at 7.30–6.94 ppm in the complexes (2–6). This is due to the electron withdrawal tendency from the aromatic ring owing to coordination with tin(IV). The methyl group attached to the tin(IV) in complexes 2 and 5 gave a singlet at 1.09 and 0.98 ppm with 2J[119Sn, 1H] coupling constant value equal to 74.4 and 77.5 Hz, respectively, supporting the five-coordinate environment around tin(IV) [29]. The three butyl groups attached to the tin(IV) moity in the organotin(IV) complex 3 gave four resonance signals, namely, 2.28–2.15 ppm (triplet, Sn–CH2–CH2–CH2–CH3), 2.14–1.73 ppm (multiplet, Sn–CH2–CH2–CH2–CH3), 1.24–1.22 ppm (multiplet, Sn–CH2–CH2–CH2–CH3), and 0.99–0.86 ppm (triplet, Sn–CH2–CH2–CH2–CH3). 1H NMR information also supported the IR data of the complexes (2–6).

3.5. 13C NMR Spectra

The 13C-{1H} NMR spectrum of free ligand (1) showed the resonance signals at 185.20, 165.32, 145.30–136.21, and 10.45 ppm are due to the δ(NH–C=S), δ(C=N), δ (aromatic ring carbon) and δ(CH3), respectively. After complexation, the carbon signals of the N=C–S group shifted to low frequency at 179.99–181.10 ppm in all the complexes (2–6) compared to ligand (1), indicating participation of the N=C–S group in coordination to tin(IV) atom. The chemical shifts of carbon in C=N and CH3 in the free ligand (1) were observed at 165.32 and 10.45 ppm which were shifted to high frequency at 168.36–178.45 and 16.44–18.70 ppm, respectively, in the complexes (2–6). This results supported the azomethine-N is coordinated to the tin(IV) atom [30]. After complexation, the δ value of carbon atoms in the aromatic ring did not have much change in the complexes (2–6) as compared to the free ligand. Besides, the butyl group attached to the organotin(IV) moiety in complex 3 gave four resonance signals at 32.78, 26.31, 24.18, and 20.11 ppm. In the 13C-{1H} NMR spectra of the organotin(IV) complexes 2 and 5, a sharp singlet resonance signal appeared at 12.80 ppm [(Sn–CH3)] and 14.97 [Sn–(CH3)2] ppm, respectively [31]. In organotin(IV) compounds, the 1J[119Sn, 13C] value is an important parameter to assess the coordination number of the Sn atom. The calculated coupling constants for dimethyltin(IV) (4) and diphenyltin(IV) (5) compounds were found to be 557 and 546 Hz, which described the penta-coordinate environment about the Sn atom in these compounds [32]. All these statements are also supported by the 1H NMR spectra analyses.

3.6. 119Sn NMR Spectra

119Sn NMR spectra can be used as an indicator of the coordination number of the tin atom. 119Sn NMR of all the complexes (2–6) shows only one resonance signals in the range of −149.60 to −185.32 ppm. 119Sn NMR values are characteristic for the five-coordinated tin atom observed in the organotin(IV) complexes (2–6) [3336].

3.7. X-Ray Crystallography Diffraction Analysis

The molecular structure of the ligand (1) with atom numbering scheme is depicted in Figure 1. The main crystal parameters are reported in Table 1. Selected bond lengths and bond angles are given in Table 2. The compound crystallizes into monoclinic crystal system with a space group of P21/c. In the title substituted thiosemicarbazone, C16H17N3OS, the hydroxy- and methyl-substituted benzene rings form dihedral angles of 9.62 (12) and 55.69 (6)°, respectively, with the central CN3S chromophore (r.m.s. deviation = 0.0117 Å) in (C16H17N3OS) (Figure 1) and the OH– and Me-benzene rings are twisted as seen in the respective dihedral angles of 9.62 (12) and 55.69 (6)°. The almost coplanarity of the central atoms is ascribed to the formation of an intramolecular hydroxyl-O–H···N-imine hydrogen bond (Table 3). The N1–N2 bond length (1.375 Å) is closer to single bond length (1.45 Å) than to double bond length (1.25 Å) [37]. The C9–S1 bond distance (1.694 Å) is close to that expected of a C=S double bond (1.60 Å) [37] and the C7–N1 bond length (1.295 Å) is nearly the same as that of the C=N double bond (1.28 Å) [38]. These bond distances are in strong support of the existence of 2-hydroxyacetophenone-2-methylphenylthiosemicarbazo in the thione form in the solid state. The H atoms of the NH groups are syn, and the conformation about the N1=C7 double bond [1.295 (4) Å] is E. The syn arrangement in (C16H17N3OS)) contrast the antiarrangement often seen in such derivatives but is readily explained in terms of the intramolecular O–H···N-imine hydrogen bond in (C16H17N3OS)) by contrast to the normally observed intramolecular N–H···N-imine hydrogen bond [39, 40]. Helical supramolecular chains along the b axis dominate the crystal packing (Figure 2 and Table 3). These arise as a result of the thione-S interacting with both N–H atoms of a neighboring molecule thereby forming six-membered hydrogen-bond-mediated rings.

Figure 1
The molecular structure of H2dampt (1) showing the atom-labelling scheme and displacement ellipsoids at the 50% probability level.
Figure 2
A view of the helical supramolecular chain aligned along the b axis in (I). The N–H···S hydrogen bonds are shown as orange dashed lines. Further stabilization to the chain is provided by C–H··· ...
Table 1
Summary of crystal data and structure refinement parameters for ligand (1).
Table 2
Selected bond lengths (Å) and bond angles (°) of ligand [H2dampt] (1).
Table 3
Hydrogen-bond geometry (Å, °)

3.8. Antibacterial Activity

The synthesized ligand (1) and its organotin(IV) complexes (2–6) were tested against Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, and Salmonella typhi bacterial strains for their antibacterial activity using agar-well diffusion method and data are shown in Table 4 and Figure 3. The doxycycline was used as a reference drug. The results showed that the substituted thiosemicarbazone ligand (1) possessed moderate antibacterial activity. The antibacterial studies of the compounds (2–6) showed relatively better activity against the selected bacteria than the free ligand (1), but low activities as compared to the reference drug. Among all the organotin(IV) derivatives, the bactericidal activities of 5 and 6 are fairly good. Complex 2 is the least active among all the organotin(IV) complexes, while complex 4 was found to be active against all the studied strains except Staphylococcus aureus. The most probable reason for this difference might be due to chelation which reduces the polarity of the central Sn atom because of the partial sharing of its positive charge with donor groups and possible π-electron delocalization within the whole chelating ring. As a result, the lipophilic nature of the central Sn atom increases, which favours the permeation of the complexes through the lipid layer of the cell membrane [41]. In addition, among the organotin(IV) complexes (2–6), complex (6) is found to be more active and it can be attributed to the presence of bulky phenyl groups which facilate binding to biological molecules π-π interactions.

Figure 3
Antibacterial activity of compounds 1–6 against various bacteria.
Table 4
Antibacterial activitya,b of the free ligand (1) and its organotin(IV) complexes 2–6 (inhibition zone in mm).

4. Conclusion

The ligand (1) and its organotin(IV) complexes (2–6) have been synthesized and fully characterized by different spectroscopic techniques. The ligand (H2dampt) exists in thione form in a solid state but it takes on a thiol form when it is in solution. All organotin(IV) complexes (2–6) of H2dampt were proposed to be five coordinated and the ligand binds to the central tin(IV) atom in dinegative tridentate form. Single crystal X-ray analysis of newly synthesized ligand (1) has been reported. The in vitro antibacterial activities of the synthesized complexes against the selected bacterial strains have been established. All compounds have been found biologically active, while the studies have confirmed that compounds 5 and 6 are more active and have the potency to be used as antibacterial agents. Trials to obtain single crystals suitable for structure determination by X-ray crystallography were in vain due to the amorphous nature of the complexes.


This work was financially supported by the Ministry of Science Technology and Innovation (MOSTI) under a Research Grant (no. 06-01-09-SF0046). The authors would like to thank Universiti Malaysia Sarawak (UNIMAS) for the facilities to carry out the research work.


1. Wang BD, Yang ZY, Lü MH, Hai J, Wang Q, Chen ZN. Synthesis, characterization, cytotoxic activity and DNA binding Ni(II) complex with the 6-hydroxy chromone-3-carbaldehyde thiosemicarbazone. Journal of Organometallic Chemistry. 2009;694(25):4069–4075.
2. Padhye S, Afrasiabi Z, Sinn E, Fok J, Mehta K, Rath N. Antitumor metallothiosemicarbazonates: structure and antitumor activity of palladium complex of phenanthrenequinone thiosemicarbazone. Inorganic Chemistry. 2005;44(5):1154–1156. [PubMed]
3. Kowol CR, Trondl R, Heffeter P, et al. Impact of metal coordination on cytotoxicity of 3-aminopyridine-2- carboxaldehyde thiosemicarbazone (Triapine) and novel insights into terminal dimethylation. Journal of Medicinal Chemistry. 2009;52(16):5032–5043. [PubMed]
4. Beraldo H, Gambino D. The wide pharmacological versatility of semicarbazones, thiosemicarbozones and their metal complexes. Mini-Reviews in Medicinal Chemistry. 2004;4(1):31–39. [PubMed]
5. Seena EB, Kurup MRP. Synthesis of mono- and binuclear dioxomolybdenum(VI) complexes derived from N(4)-substituted thiosemicarbazones: X-ray crystal structures of [(MoO2L1)2], [MoO2L1py] and [MoO2L2py] Polyhedron. 2007;26(14):3595–3601.
6. Maurya MR, Kumar A, Abid M, Azam A. Dioxovanadium(V) and μ-oxo bis[oxovanadium(V)] complexes containing thiosemicarbazone based ONS donor set and their antiamoebic activity. Inorganica Chimica Acta. 2006;359(8):2439–2447.
7. Alomar K, Khan MA, Allain M, Bouet G. Synthesis, crystal structure and characterization of 3-thiophene aldehyde thiosemicarbazone and its complexes with cobalt(II), nickel(II) and copper(II) Polyhedron. 2009;28(7):1273–1280.
8. Vieites M, Otero L, Santos D, et al. Platinum-based complexes of bioactive 3-(5-nitrofuryl)acroleine thiosemicarbazones showing anti-Trypanosoma cruzi activity. Journal of Inorganic Biochemistry. 2009;103(3):411–418. [PubMed]
9. Tarassoli A, Sedaghat T. In: Organometallic Chemistry Research Perspectives. Irwin RP, editor. New York, NY, USA: Nova Science; 2007. p. 7.
10. Arakawa Y. In: Chemistry of Tin. 2nd edition. Smith PJ, editor. London, UK: Blackie; 1998.
11. Carcelli M, Pelizzi C, Pelizzi G, Mazza P, Zani F. The different behaviour of the di-2-pyridylketone 2-thenoylhydrazone in two organotin compounds. Synthesis, X-ray structure and biological activity. Journal of Organometallic Chemistry. 1995;488:55–61.
12. Gielen M. Organotin compounds and their therapeutic potential: a report from the Organometallic Chemistry Department of the Free University of Brussels. Applied Organometallic Chemistry. 2002;16(9):481–494.
13. Gielen M. Tin-based antitumour drugs. Coordination Chemistry Reviews. 1996;151:41–51.
14. Nath M, Pokharia S, Yadav R. Organotin(IV) complexes of amino acids and peptides. Coordination Chemistry Reviews. 2001;215(1):99–149.
15. Singh MS. Synthesis and characterisation of organotin(IV) derivatives of benzilmonohydrazone. Indian Journal of Chemistry—Section A. 1998;37(10):911–914.
16. Katsoulakou E, Tiliakos M, Papaefstathiou G, et al. Diorganotin(IV) complexes of dipeptides containing the α-aminoisobutyryl residue (Aib): preparation, structural characterization, antibacterial and antiproliferative activities of [(n-Bu)2Sn(H-1L)] (LH = H-Aib-L-Leu-OH, H-Aib-L-Ala-OH) Journal of Inorganic Biochemistry. 2008;102(7):1397–1405. [PubMed]
17. Basu Baul TS, Masharing C, Ruisi G, et al. Self-assembly of extended Schiff base amino acetate skeletons, 2-{[(2Z)-(3-hydroxy-1-methyl-2-butenylidene)]amino}phenylpropionate and 2-{[(E)-1-(2-hydroxyaryl)alkylidene]amino}phenylpropionate skeletons incorporating organotin(IV) moieties: synthesis, spectroscopic characterization, crystal structures, and in vitro cytotoxic activity. Journal of Organometallic Chemistry. 2007;692(22):4849–4862.
18. Salam MA, Affan MA, Ahmad FB, Hitam RB, Gal Z, Oliver P. Synthesis and characterization of tin(IV)/organotin(IV) complexes with 2-benzoylpyridine-N(4)-cyclohexylthiosemicarbazone [HBPCT]: X-ray crystal structure of [SnCl3(BPCT)] Journal of Coordination Chemistry. 2011;64(14):2409–2418.
19. Affan MA, Salam MA, Ahmad FB, Ismail J, Shamsuddin MB, Ali HM. Synthesis and spectroscopic characterization of organotin(IV) complexes with 2-benzoylpyridine-N(4)-cyclohexylthiosemicarbazone (HBPCT): X-ray crystal structure of [PhSnCl2(BPCT)] Inorganica Chimica Acta. 2011;366(1):227–232.
20. Armarego WLF, Perrin DD. Purification of Laboratory Chemicals. 4th edition. Oxford, UK: Butterworth Heinemann; 1996.
21. Rahman A, Choudry MI, Thomsen WJ. Bioassay Techniques for Drug Development. Amsterdam, The Netherlands: Harwood Academic; 2001.
22. Sharaby CM. Synthesis, spectroscopic, thermal and antimicrobial studies of some novel metal complexes of Schiff base derived from [N1-(4-methoxy-1,2,5-thiadiazol-3-yl)sulfanilamide] and 2-thiophene carboxaldehyde. Spectrochimica Acta—Part A. 2007;66(4-5):1271–1278. [PubMed]
23. Rebolledo AP, De Lima GM, Gambi LN, et al. Tin(IV) complexes of 2-benzoylpyridine N(4)-phenylthiosemicarbazone: spectral characterization, structural studies and antifungal activity. Applied Organometallic Chemistry. 2003;17(12):945–951.
24. Maurya MR, Jayaswal MN, Puranik VG, Chakrabarti P, Gopinathan S, Gopinathan C. Dioxomolybdenum(VI) and dioxotungsten(VI) complexes of isomeric ONO donor ligands and the X-ray crystal structure of [MoO2(o-OC6H4CH=NCH2C6H4O)(MeOH)]2·MeOH. Polyhedron. 1997;16(23):3977–3983.
25. Rajan OA, Chakravorty A. Molybdenum complexes. 1. Acceptor behavior and related properties of MoVIO2(tridentate) systems. Inorganic Chemistry. 1981;20(3):660–664.
26. Costa RFF, Rebolledo AP, Matencio T, et al. Metal complexes of 2-benzoylpyridine-derived thiosemicarbazones: structural, electrochemical and biological studies. Journal of Coordination Chemistry. 2005;58(15):1307–1319.
27. Garg BS, Prathapachandra Kurup MR, Jain SK, Bhoon YK. Spectroscopic studies on copper(II) complexes derived from a substituted 2-acetylpyridine thiosemicarbazone. Transition Metal Chemistry. 1988;13(4):309–312.
28. Mendes IC, Moreira JP, Mangrich AS, Balena SP, Rodrigues BL, Beraldo H. Coordination to copper(II) strongly enhances the in vitro antimicrobial activity of pyridine-derived N(4)-tolyl thiosemicarbazones. Polyhedron. 2007;26(13):3263–3270.
29. Lockhart TP, Manders WF. Structure determination by NMR spectroscopy. Correlation of |2J(119Sn,1H)| and the Me-Sn-Me angle in methyltin(IV) compounds. Inorganic Chemistry. 1986;25(7):892–895.
30. Labisbal E, Rodríguez L, Sousa-Pedrares A, et al. Synthesis, characterisation and X-ray structures of diorganotin(IV) and iron(III) complexes of dianionic terdentate Schiff base ligands. Journal of Organometallic Chemistry. 2006;691(7):1321–1332.
31. Yin HD, Chen SW. Synthesis and characterization of organotin(IV) compounds with Schiff base of o-vanillin-2-thiophenoylhydrazone. Journal of Organometallic Chemistry. 2006;691(13):3103–3108.
32. Lockhart TP, Davidson F. Methyltin(IV) structure determination by NMR. X-ray and NMR structural analyses of three Me2Sn(chelate)2 compounds bearing five-membered chelate rings. Organometallics. 1987;6(12):2471–2478.
33. Holeček J, Handlir K, Nadvornik M, Lycka A. 13C and 119Sn NMR study of some triphenyl tin(IV) carboxylates. Journal of Organometallic Chemistry. 1983;258:147–153.
34. Holeček J, Nadvornik M, Handlir K, Lycka A. 13C and 119Sn NMR spectra of Di-n-butyltin(IV) compounds. Journal of Organometallic Chemistry. 1986;315:299–308.
35. Sarkar B, Choudhury AK, Roy A, et al. Crystal structure, anti-fungal activity and phytotoxicity of diorganotin compounds of dihalo-substituted [(2-hydroxyphenyl) methylideneamino]thiourea. Applied Organometallic Chemistry. 2010;24(12):842–852.
36. Din IU, Mazhar M, Molloy KC, Khan KM. Synthesis, structural characterization of some germanium substituted chiral diethyltin derivatives. Journal of Organometallic Chemistry. 2006;691(8):1643–1648.
37. Huheey JE, Keiter EA, Keiter RL. Inorganic Chemistry, Principles of Structure and Reactivity. 4th edition. New York, NY, USA: Harper Collins College; 1993.
38. March J. Advanced Organic Chemistry, Reactions, Mechanisms and Structure. 4th edition. New York, NY, USA: John Wiley & Sons; 1992.
39. Normaya E, Farina Y, Halim SNA, Tiekink ERT. 3-(2-Hydroxyphenyl)-1-{(E)-[1-(pyrazin-2-yl)ethylidene]amino}thiourea monohydrate. Acta Crystallographica Section E. 2011;67:o943–o944. [PMC free article] [PubMed]
40. Salam MA, Affan MA, Ahmad FB, Ng SW, Tiekink ERT. 1-Cyclohexyl-3-{(E)-[1-(pyridin-2-yl)ethylidene]amino}thiourea. Acta Crystallographica Section E. 2010;67, article o955 [PMC free article] [PubMed]
41. Srivastava RS. Pseudotetrahedral Co(II), Ni(II) and Cu(II) complexes of N1-(O-chlorophenyl)-2-(2′,4′-dihydroxyphenyl)-2-benzylazomethine their fungicidal and herbicidal activity. Inorganica Chimica Acta. 1981;56:65–67.

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