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Mol Pain. 2012; 8: 18.
Published online 2012 March 22. doi:  10.1186/1744-8069-8-18
PMCID: PMC3352126

Acute morphine activates satellite glial cells and up-regulates IL-1β in dorsal root ganglia in mice via matrix metalloprotease-9

Abstract

Background

Activation of spinal cord glial cells such as microglia and astrocytes has been shown to regulate chronic opioid-induced antinociceptive tolerance and hyperalgesia, due to spinal up-regulation of the proinflammatory cytokines such as interleukin-1 beta (IL-1β). Matrix metalloprotease-9 (MMP-9) has been implicated in IL-1β activation in neuropathic pain. However, it is unclear whether acute opioid treatment can activate glial cells in the peripheral nervous system. We examined acute morphine-induced activation of satellite glial cells (SGCs) and up-regulation of IL-1β in dorsal root ganglia (DRGs), and further investigated the involvement of MMP-9 in these opioid-induced peripheral changes.

Results

Subcutaneous morphine injection (10 mg/kg) induced robust peripheral glial responses, as evidenced by increased GFAP expression in DRGs but not in spinal cords. The acute morphine-induced GFAP expression is transient, peaking at 2 h and declining after 3 h. Acute morphine treatment also increased IL-1β immunoreactivity in SGCs and IL-1β activation in DRGs. MMP-9 and GFAP are expressed in DRG neurons and SGCs, respectively. Confocal analysis revealed a close proximity of MMP-9 and GFAP immunostaining. Importantly, morphine-induced DRG up-regulation of GFAP expression and IL-1β activation was abolished after Mmp9 deletion or naloxone pre-treatment. Finally, intrathecal injections of IL-1β-selective siRNA not only reduced DRG IL-1β expression but also prolonged acute morphine-induced analgesia.

Conclusions

Acute morphine induces opioid receptors- and MMP-9-dependent up-regulation of GFAP expression and IL-1β activation in SGCs of DRGs. MMP-9 could mask and shorten morphine analgesia via peripheral neuron-glial interactions. Targeting peripheral glial activation might prolong acute opioid analgesia.

Background

Mounting evidence indicates that activation of spinal cord glial cells such as microglia and astrocytes plays a crucial role in the pathogenesis of chronic pain [1-7]. In particular, chronic opioid exposure induces profound changes in spinal cord microglia and astrocytes [8-10]. Upon activation spinal glial cells produce multiple proinflammatory cytokines such as TNF-α, IL-1β, and IL-6 to antagonize morphine analgesia and promote morphine tolerance [11-14], via sensitization of spinal cord dorsal horn neurons [15-17]. For example, IL-1β has been shown to counteract opioid-induced analgesia following both chronic and acute administration of morphine [18]. While intrathecal injection of the inteurleukin-1 receptor (IL-1R) antagonist potentiates acute morphine analgesia [11], intrathecal administration of IL-1β induces heat hyperalgesia [15]. Of interest genetic polymorphism of IL-1R antagonist has been implicated in the variation in postoperative morphine consumption [19].

Our previous study has shown that matrix metalloproteinase 9 (MMP-9) plays an important role in neuroinflammation and neuropathic pain development in part through the activation (active cleavage) of IL-1β [20]. MMP-9 has also been shown to regulate the phenotype and proliferation of peripheral [21] and central [20] glial cells. In a parallel study, we also showed that acute morphine induced a rapid up-regulation of MMP-9 in primary sensory neurons in the dorsal root ganglia (DRGs), masking morphine-induced analgesia (Liu et al., unpublished data). However, the molecular and cellular mechanisms by which MMP-9 suppresses/shortens acute opioid analgesia are unclear.

Satellite glial cells (SGCs) are peripheral glial cells and form a continuous layer around primary sensory neurons within DRGs and trigeminal ganglia (TGs). SGCs have been shown to regulate neuronal homeostasis and neurotransmission in DRGs and TGs [22]. Increasing evidence suggests that SGCs exhibit marked morphological and biochemical changes following peripheral nerve injury and inflammation [23-27]. Peripheral inflammation suppresses inward rectifying potassium currents and increases IL-1β expression in SGCs of TG, leading to neuronal firing and trigeminal pain [28,29]. Chemokine signaling in DRG SGCs and neurons was shown to regulate chronic opioid-induced hyperalgesia [30]. However, it is unclear whether and how SGCs are activated in DRGs following acute opioid treatment. Our data demonstrated that a single subcutaneous morphine injection could induce MMP-9 and opioid receptors-dependent activation of SGCs and IL-1β in DRGs, highlighting the importance of peripheral neuronal-satellite glial interactions for the control of acute opioid analgesia.

Results

Subcutaneous morphine induces GFAP expression in DRGs but not in spinal cords

The first question we addressed is whether acute morphine-induced acute analgesia is associated with peripheral glial responses in DRGs. We used GFAP as a marker for SGCs and performed immunohistochemistry to examine GFAP expression in DRGs, 2 h after saline and morphine injection when morphine analgesia declines. In DRGs of saline-treated control mice, only weak GFAP staining was observed (Figure (Figure1A).1A). Strikingly, the intensity of GFAP staining was markedly increased in DRGs of morphine-treated mice (Figure (Figure1B1B).

Figure 1
Subcutaneous morphine increases GFAP immunoreactivity in DRG SGCs. (A, B) GFAP immunoreactivity in DRGs from saline (A) or morphine (B, 10 mg/kg, s.c.) treated mice at 2 h after the injections. Left panels are images with high contrast showing all DRG ...

Next, we performed qPCR analysis to quantify the GFAP mRNA levels in DRGs and spinal cords at different times of morphine injection. Acute morphine increased GFAP mRNA expression in DRGs [F(3,12) = 30.9, P < 0.0001, One-Way ANOVA], which peaked at 2 h (4.5 fold of control, P < 0.05, n = 4) and declined but still remained elevated at 3 h (2.6 fold of control, P < 0.05, n = 4) (Figure (Figure2A).2A). In contrast, acute morphine did not change GFAP mRNA expression in spinal cord dorsal horns at all the time points we examined (F(3,12) = 0.51, P = 0.682, One-Way ANOVA] (Figure (Figure2B).2B). Thus, it appears that acute morphine only causes reaction of peripheral glia (SGCs) but not of central glia, since GFAP is a hallmark for astrocytes in the CNS.

Figure 2
Subcutaneous morphine induces GFAP mRNA expression in DRGs but not in spinal cords. (A, B) Real-time RT-PCR showing the time course of GFAP expression in lumbar DRGs (A) and spinal cord dorsal horns (B) after subcutaneous morphine injection (10 mg/kg, ...

MMP-9 is required for subcutaneous morphine-induced GFAP expression in DRG SGCs

GFAP-positive SGCs were found to form ring-like staining around 5.5% DRG neurons of saline-treated mice (Figure 3A, B). This percentage increased to 17.1 at 2 h after morphine injection (Figure 3A, B; P < 0.05, n = 6). To determine the contribution of MMP-9 to morphine-elicited SGC reaction, we examined GFAP expression in Mmp9 null mice (Mmp9-/-). Compared to wild-type control mice, morphine-induced increase in ring-like staining of GFAP was abolished in Mmp9-/- mice, and only 7.6% DRG neurons showed GFAP-ring (Figure 3A, B, P <0.05, n = 6). Furthermore, qPCR analysis revealed that morphine-induced DRG increase in GFAP mRNA expression was also abrogated in Mmp9-/- mice compared to that of the wild-type mice (Figure (Figure3C;3C; 0.9 fold of vehicle, P <0.05, n = 4). However, the basal expression levels of GFAP mRNA in the wild-type and Mmp9-/- mice were very similar (P = 0.2468, Mann-Whitney test, n = 4). Together, these data suggest that MMP-9 is essential for GFAP up-regulation in DRG-SGCs following acute morphine treatment.

Figure 3
MMP-9 is required for subcutaneous morphine-induced GFAP expression in SGCs of DRGs. (A) Left panels: DAPI nuclei staining of all cells in DRG sections of saline (up panel) and morphine (2 h, middle and low panels) treated wild-type (WT) mice and morphine-treated ...

In a parallel study, we demonstrated MMP-9 up-regulation in DRG neurons after acute morphine treatment (Liu et al., unpublished data). MMP-9 could be secreted to extracellular space to trigger the reaction of surrounding SGCs via neuronal-glial interaction. Double staining of MMP-9 and GFAP and confocal microscopy examination revealed that MMP-9 and GFAP-labeled structures were in very close proximity but not overlapped (Figure (Figure3D),3D), providing a structural base for MMP-9-initiated neuronal-glial interaction in DRGs.

Subcutaneous morphine induces IL-1β activation in DRG SGCs via MMP-9

Chronic morphine but not acute morphine was shown to increase IL-1β levels in the spinal cord [11]. We set out to test if acute morphine would induce IL-1β expression and activation in DRGs. Low level of IL-1β expression was observed in DRGs of saline-treated wild-type animals: only 5.4% DRG neurons were surrounded by IL-1β-expressing staining in the control animals. However, this percentage increased to 22.7 in morphine-treated wild-type mice (Figure 4A, B; P < 0.05, n = 6). Notably, this increase was abrogated in Mmp9-/- mice, and only 7.4% DRG neurons were surrounded by IL-1β-expressing satellite cells (Figure 4A, B; P < 0.05, n = 6). However, acute morphine did not alter the expression of the IL-1β mRNA (Figure (Figure4C4C).

Figure 4
MMP-9 is required for subcutaneous morphine-induced IL-activation in DRGs. (A) DAPI nuclei staining (left panels) and IL-1β immunostaining (right panels) in DRG sections of saline (upper panels) and morphine (middle panels) treated ...

Next, we conducted Western blot analysis to check the activation of IL-1β in DRGs after morphine treatment. Morphine produced no significant change of the 28 kDa band of IL-1β, the non-mature form of IL-1β (Figure (Figure4D).4D). In contrast, morphine induced a significant increase of the 17 kDa band of IL-1β, the active form of IL-1β, in DRGs of wild-type mice (Figure 4D, E, 1.4 fold of control, P < 0.05, n = 4). Notably, this increase of the active form of IL-1β was completely abolished in Mmp9-/- mice (Figure 4D, E).

To determine the cellular localization of IL-1β in DRGs, we carried out triple staining for GFAP, IL-1β, and DAPI (nuclear marker). We found a clear co-localization of GFAP and IL1β in cytoplasm of SGCs of morphine-treated mice (Figure (Figure5).5). Together, our results suggest that MMP-9 is required for the morphine-induced IL-1β activation in SGCs.

Figure 5
Co-localization of GFAP and IL-1β in SGCs of DRGs. (A) Triple staining showing co-localization of GFAP (green), IL-1β (red), and DAPI (blue) in DRG-SGCs of morphine (2 h)-treated mice. Star and arrows indicate neurons and satellite glia, ...

Intrathecal opioids increase GFAP expression and IL-1β activation in DRGs but not in spinal cords

To determine whether morphine would directly act on DRG cells to produce the observed effects, we intrathecally injected morphine or remifentanil, a potent fast-acting synthetic opioid. Single intrathecal injection of morphine (10 nmol) and remifentanil (1 noml) elicited significant increases in GFAP levels [(F(2,9) = 13.1, P = 0.002, One-Way ANOVA] and IL1β (17 kDa) levels [(F(2,9) = 8.6, P = 0.008, One-Way ANOVA] in DRGs (Figure (Figure6A).6A). In contrast, intrathecal morphine and remifentanil failed to induce GFAP and IL-1β (17 kDa) expression in spinal cord dorsal horns (Figure (Figure6B).6B). These results indicate that DRGs are primary target for acute opioid-induced GFAP expression and IL-1β activation.

Figure 6
Intrathecal injection of morphine and remifentanil increases GFAP expression and IL-1β activation in DRGs but not in spinal cords. (A) Western blotting showing the expression of GFAP and IL-1β (17 kDa cleaved form) in DRGs after intrathecal ...

Intrathecal injection of IL-1β-targeting siRNA prolongs morphine analgesia

IL-1β antagonist has been shown to potentiate acute morphine analgesia and reduce morphine tolerance [11,31]. We further evaluated the role of IL-1β in acute opioid analgesia using a different strategy to knock down DRG-IL-1β expression via intrathecal route. Compared to non-targeting control siRNA, IL-1β siRNA treatment significantly increased tail-flick latency at 2 h (Figure (Figure7A,7A, P < 0.05, Bonferroni post hoc test, n = 6) but not at 1 h (Figure (Figure7A,7A, P > 0.05). It is suggested that IL-1β siRNA treatment could prolong opioid analgesia.

Figure 7
Intrathecal injections of IL-1β siRNA prolong morphine analgesia and reduce IL-1β expression in DRGs. (A) Acute morphine-induced analgesia is prolonged after intrathecal injections of IL-1β siRNA (3 μg, 24 and 2 h prior ...

We also examined the knockdown effects of IL-1β siRNA on IL-1β expression in DRG and spinal cord tissues collected 2 h after morphine injection. Notably, morphine-induced IL-1β expression in DRGs was reduced by 40% after intrathecal IL-1β siRNA treatment, compared with control siRNA (Figure (Figure7B,7B, P <0.05, Mann-Whitney test, n = 6). However, the same IL-1β siRNA treatment (i.t.) did not produce significant reduction of IL-1β expression in spinal cord dorsal horns at 2 h after morphine injection (Figure (Figure7C,7C, P > 0.05, Mann-Whitney test, n = 6). One possible explanation for the differential knockdown of DRG vs. spinal cord IL-1β by intrathecal siRNA treatment is that DRG IL-1β is inducible after morphine treatment, therefore, more sensitive to siRNA treatment, as shown in our previous study [32,33]. In contrast, knockdown of constitutive IL-1β expression in the spinal cord may require high doses and additional injections of siRNA. Since the IL-1β siRNA treatment only reduced DRG IL-1β, which is expressed in SGCs, we hypothesize that IL-1β expressed by peripheral DRG glia (SGCs) plays a role in masking/diminishing morphine-induced analgesia.

Morphine induces GFAP up-regulation and IL-1β activation in DRG SGCs via opioid receptors

Morphine could induce SGC activation via opioid receptors-dependent and independent mechanisms, such as activation of toll-like receptors [34]. To address this issue, we investigated the effects of the opioid receptor antagonist naloxone on the morphine-induced GFAP expression and IL-1β activation. Pre-treatment of naloxone (10 mg/kg, s.c.), 10 min prior to the morphine injection, completely blocked the morphine-evoked GFAP mRNA expression (Figure (Figure8A,8A, P <0.05, One-Way ANOVA) and IL-1β activation (Figure 8B, C, P <0.05, One-Way ANOVA) in DRGs.

Figure 8
Naloxone blocks subcutaneous morphine-induced GFAP expression and IL-1β activation in DRGs. (A) Real-time RT-PCR showing GFAP mRNA expression in DRGs after subcutaneous morphine injection (10 mg/kg, 2 h) with or without naloxone pre-treatment ...

Discussion

In the present study, together with another study in an accompanying paper (Liu et al., unpublished data), we provided several lines of evidence that support an important role of DRG satellite glial cells (SGCs) in masking and shortening opioid analgesia, via neuron-glial interactions in DRGs (Figure (Figure9).9). (1) Subcutaneous or intrathecal opioid (morphine and remifentanil) induced rapid and transient MMP-9 expression (1-3 h) in DRG neurons but not in the spinal cord (Liu et al., unpublished data). (2) Subcutaneous or intrathecal opioid induced GFAP expression (at 2 h) in DRG SGCs but not in the spinal cord (Figures (Figures1,1, ,2,2, and and6).6). (3) Subcutaneous or intrathecal opioid induced IL-1β activation (at 2 h) in DRG SGCs but not in spinal cords (Figures (Figures44 and and6).6). (4) The morphine-induced SGC activation (GFAP expression and IL-1β activation) in DRGs was abolished after Mmp9 deletion and naloxone pre-treatment (Figures (Figures3,3, ,4,4, and and8).8). (5) Acute morphine-induced analgesia was enhanced and prolonged after Mmp9 deletion or by MMP-9 inhibition via intrathecal route (Liu et al., unpublished data). (6) Intrathecal treatment of IL-1β siRNA (using a mild protocol with 2 injections) produced IL-1β knockdown in DRGs (but not in spinal cords) and also prolonged morphine-elicited analgesia (Figure (Figure7).7). Based on these findings, we postulate that neuronal MMP-9 expression and subsequent MMP-9 release following acute morphine treatment triggers the activation of SGCs (IL-1β activation), diminishing the analgesic efficacy of opioid (Figure (Figure99).

Figure 9
Schematic of the working hypothesis illustrating how acute morphine induces SGCs activation in DRGs to mask morphine analgesia. Acute morphine produces analgesia via μ opioid receptors (MOR). Acute morphine also induces MMP-9 up-regulation in ...

To the best of our knowledge, this is the first report showing that acute morphine could induce satellite glial activation in DRGs. We found significant increases in both mRNA and protein levels of the satellite glial marker GFAP after a single subcutaneous injection of morphine (Figures (Figures11 and and2),2), indicating a peripheral glial reaction by acute morphine. In sharp contrast, previous studies have focused on chronic morphine treatment and central glial activation. For example, chronic morphine induced up-regulation of the microglial markers CD11b and IBA1 and the astrocyte marker GFAP in the spinal cord [8,9,12,35,36]. In particular, chronic morphine activates p38 MAP kinase in spinal cord microglia to promote morphine tolerance [10,36-38]. However, we failed to detect increases in the expression of spinal glial markers such as the astrocyte marker GFAP (Figures (Figures22 and and6)6) and the microglial marker IBA-1 (data not shown) after single subcutaneous and intrathecal injections of morphine, suggesting that acute morphine primarily induces peripheral glial responses in DRGs.

SGCs in the DRG are tightly associated with sensory neurons via gap junction; and gap junction communication between SGCs and neurons is greatly enhanced in persistent pain conditions [39,40]. Neuron-glial interactions in DRGs may involve purinergic signaling [41,42]. After nerve injury, chronic inflammation, and DRG compression, SGCs exhibit robust up-regulation of GFAP [43-45]. Nerve injury-induced SGC reaction depends on neuronal activity and local inflammation [23,46]. Our data showed that morphine-induced GFAP induction in SGCs required MMP-9 (Figure (Figure3).3). As an extracellular matrix protein, MMP-9 could be released from DRG neurons following morphine stimulation and retain in extracellular space between neurons and satellite cells. MMP-9 may cleave extracellular matrix proteins to induce satellite glial reaction and GFAP up-regulation (Figure (Figure8).8). Of note MMP-9 immunostaining in neurons had very close proximity to GFAP immunostaining in SGCs (Figure (Figure3D).3D). MMP-9 has been shown to regulate phenotypic remodeling and proliferation of peripheral glial cells such as Schwann cells via signaling of IGF-1, ErbB receptors, and ERK [21].

In particular, our data demonstrated that MMP-9 could drive IL-1β activation after acute morphine. Acute morphine induced IL-1β activation in the DRG but not in the spinal cord (Figures (Figures44 and and6).6). Similarly, a previous study failed to show IL-1β increase either in lumbar spinal cord or in CSF after acute morphine injection [11]. As previously reported [32,47,48], we observed IL-1β expression by DRG SGCs. However, we should not rule out the possibility that IL-1β could also be expressed in neurons by testing different IL-1β antibodies [32,49]. Primary sensory neurons particularly nociceptors express IL-1 receptors, and notably, activation of these receptors by IL-1β can rapidly activate nociceptors to generate action potentials and elicit pain hypersensitivity [50]. It appears that IL-1β increases the excitability of sensory neurons via increasing sodium currents and decreasing potassium currents [48,50,51]. Importantly, intrathecal interleukin-1 receptor antagonist potentiates both intrathecal and systemic morphine-induced analgesia [11,31]. Since IL-1β activation following acute morphine was primarily found in the DRG, we postulate that intrathecal interleukin-1 receptor antagonist may also enhance opioid analgesia by blocking IL-1β signaling in DRGs. This notion is strongly supported by our siRNA experiment, showing that morphine analgesia was prolonged after IL-1β knockdown in DRGs by selective siRNA treatment (Figure (Figure77).

Importantly, Mmp9 deletion resulted in a complete loss of morphine-induced IL-1β activation in the DRG (Figure (Figure4).4). IL-1β is activated via cleavage from its precursor (≈ 31 kDa) and this activation is not only mediated by caspase-1 (i.e. IL-1β converting enzyme) but also mediated by other enzymes such as trypsin, elastase, collagenase, and cathepsin G, and MMP-9 [52-54]. MMP-9 can cause direct activation of IL-1β (generation of 17 kDa form) in a cell-free system [53]. Notably, caspase-1-deficient mice can still produce bioactive IL-1β and mediate IL-1β-dependent reactions under several pathological conditions [55]. Consistently, nerve injury-induced DRG-IL-1β activation is abolished in Mmp9-/- mice, whereas intrathecal MMP-9 increases IL-1β activation in DRGs [32]. However, we should not exclude the possibility that at very high concentrations MMP-9 may also cause IL-1β degradation and inactivation via direct cleavage of IL-1β at different sites [56].

In summary, opioids are still the most used analgesics for the treatment of moderate to severe pain, despite their well-known side effects. Increasing evidence shows that spinal cord glial cells and proinflammatory cytokines contribute to chronic opioid-induced antinociceptive tolerance and hyperalgesia via neuronal-glial interactions in the spinal cord [12,13]. We have demonstrated that acute morphine also induces peripheral satellite glial cell (SGC) activation in the DRG, which is triggered by the protease MMP-9. Morphine-induced MMP-9 up-regulation in DRG neurons could mask morphine analgesia via neuronal-glial interactions. Specifically, MMP-9 caused IL-1β activation, leading to hyperexcitability of primary sensory neurons to mask morphine analgesia (Figure (Figure9).9). Thus, opioids not only produce excitatory effects in the spinal cord via postsynaptic [57,58] and presynaptic mechanisms [59], they could further elicit excitatory effects at DRG level via MMP-9-triggered peripheral neuronalglial interactions. Targeting peripheral neuronal-glial interactions in DRGs may prolong the analgesic efficacy of opioids.

Methods

Animals

All experiments were performed in accordance with the guidelines of the National Institutes of Health and the International Association for the Study of Pain. All animals were used under Harvard Medical School Animal Care institutional guidelines. Adult male mice (25-35 g) were used for behavioral and biochemical studies, including CD1 mice (Charles River Laboratories, Wilmington, MA), Mmp9 konckout (Mmp9-/-) mice with FVB background, and FVB control wild-type mice. Mmp9 knockout (KO) mice and FVB wild-type mice were obtained from Jackson Laboratories (The Jackson Laboratory, Bar Harbor, ME). The KO mice are viable, fertile, and maintained in FVB background for more than 5 generations. Mice that are homozygous null for the Mmp9 gene were used in this study. As previously described, they do not show any difference in overall weight and behavior compared with wild-type mice [32]. Animals were housed in a 12 h light/dark room with access to food and water ad libitum.

Drugs and administration

We purchased morphine sulphate and remifentanil from Hospira (Lake Forest, IL) and naloxone form Sigma (St. Louis, MO). Drugs were freshly prepared in saline and administered subcutaneously in the lower back (10 mg/kg for morphine and naloxone) or intrathecally (10 nmol for morphine and 1 nmol for remifentanil). The IL-1β (GCUCCGAGAUGAACAACAA) and control siRNAs (GACUUCGCGGGACACAUGA) were purchased from Dharmacon (Dharmacon, Inc., Chicago, IL). siRNA was dissolved in RNase-free water at the concentration of 1 μg/μl as stock solution, and mixed with polyethyleneimine (PEI, Fermentas Inc., Glen Burnie, MD), 10 min before injection, to increase cell membrane penetration and reduce the degradation. PEI was dissolved in 5% glucose, and 1 μg of siRNA was mixed with 0.18 μl of PEI. siRNA (3 μg) was intrathecally injected 24 and 2 h before the morphine injection. Of note, agents with different chemical properties have been shown to affect DRG cells via intrathecal route, including small molecules such as MAP kinase and MMP-9 inhibitors [32,60] and large molecules such as growth factors and peptides [61,62], as well as antisense oligodeoxynucleotides [63,64] and siRNAs [33,65]. Intrathecal injection of p38 inhibitor can rapidly inhibit p38 activation in DRG neurons within half hour [66]. Thus, intrathecal delivery of siRNA should affect cells both in DRGs and spinal cords.

Behavioral test

All animals (n = 6 mice per group) were habituated to testing environment for at least 2 days before baseline testing. Morphine analgesia was evaluated by measuring the tail-flick latencies in hot water [67]. Briefly, tail-flick test was performed by gently holding the mouse wrapped with a terry towel and kept tail exposed. Then one third of the length of the tail was immersed into the 52°C hot water, and the response latency was defined as removal of the whole tail from the water. A maximum cut-off value of 10 seconds was set to avoid thermal injury.

Immunohistochemistry

Two hours after morphine or vehicle (saline) injection, animals (n = 6 mice per group) were terminally anesthetized with isoflurane and perfused through the ascending aorta with PBS, followed by 4% paraformaldehyde with 1.5% picric acid in 0.16 M PB. After the perfusion, DRGs (L4/L5) were removed and postfixed in the same fixative overnight. DRG sections (12 μm) were cut in a cryostat and processed for immunofluorescence. All the sections were blocked with 10% goat serum, and incubated over night at 4°C with the primary antibodies against GFAP (mouse, 1:2000, Millipore, Billerica, MA), MMP-9 (1:1000, rabbit, Millipore), or IL-1β (1:1000, rabbit, Millipore; 1:500, goat, R&D System, Minneapolis, MN) with 5% goat serum. The sections were then incubated for 1 h at room temperature with Cy3- or FITC-conjugated secondary antibody (1:400, Jackson Immunolab, West Grove, PA) with 1% goat serum. DAPI (4',6-diamidino-2-phenylindole; Sigma) staining was used to determine the cell nuclei. For double immunofluorescence, sections were incubated with a mixture of polyclonal and monoclonal primary antibodies followed by a mixture of FITC- and CY3-congugated secondary antibodies [68]. The stained sections were examined under a Nikon fluorescence microscopy, and images were captured with a CCD camera (SPOT, Diagnostic Instruments). To obtain high-resolution images, confocal images were captured from some DRG section with a Zeiss LSM 510 META upright confocal microscope. All images were analyzed with NIH Image software or Adobe Photoshop.

Western blot

Animals (n = 4-6 mice per group) were terminally anesthetized with isoflurane at 1, 2, and 3 h after morphine injection and transcardially perfused with PBS. DRGs (L4/L5) and spinal cord segments (dorsal part of L4-L5) were rapidly removed and homogenized in a lysis buffer containing a cocktail of protease inhibitors and phosphatase inhibitors [69]. The protein concentrations were determined by BCA Protein Assay (Pierce Biotechnology, Inc., Rockford, IL). Twenty micrograms of proteins were loaded for each lane and separated on SDS-PAGE gel (4-15%, Bio-Rad). After the transfer, the blots were incubated overnight at 4°C with polyclonal antibody against GFAP (mouse, 1:1000, Millipore) or IL-1β (1:1000, rabbit, Millipore). For loading control, the blots were probed with β-tubulin or GAPDH antibody (mouse, respectively 1:2000 and 1:20000, Sigma).

Quantitative real-time PCR (qPCR)

Animals (n = 4 per group) were terminally anesthetized with isoflurane at 1, 2, and 3 h after morphine injection. DRGs (L4/L5) and spinal cord dorsal horn segments (L4-L5) were rapidly dissected and total RNA from each animal was isolated using RNeasy Plus Mini kit (Qiagen, Valencia, CA). One microgram of RNA was reverse transcribed (RT) using Omniscript reverse transcriptase according to the protocol of the manufacturer (Qiagen). The sequences for the forward and reverse primers of GFAP and GAPDH are included in Table Table1.1. SYBR-green qPCR analyses were performed using the Opticon real-time PCR Detection System (Bio-Rad, Hercules, CA) as described previously [70]. Briefly, qPCR amplification reactions contained RT products, 7.5 μl of 2X iQSYBR-green mix (Bio-Rad), 300 nM of forward and reverse primers completed with nanopure water for a final volume of 15 μl. The thermal cycling conditions were: 3 min at 95°C for the polymerase activation, 45 cycles of 10 s at 95°C for denaturation, and 30 s at 60°C for annealing and extension, followed by a DNA dissociation curve for the determination of the amplicon specificity. Analyses were carried out in triplicates and included the different primer efficiencies obtained by a standard curve method.

Table 1
Sequences of the primer sets for RT-PCR

Statistical analyses

All results are presented as means ± SEM. Differences between means were tested for significance using Mann-Whitney test, 1-way or 2-way ANOVA followed by Bonferroni post hoc test. Significance was determined at a level of P < 0.05.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

TB, YCL, and ZZX performed biochemical and histochemical experiments; TL performed behavioral testing; RRJ, TB, and TL designed the experiments; and RRJ and TB wrote the manuscript. All authors read and approved the final manuscript.

Acknowledgements

This study was supported by NIH grants DE17794, NS54932, NS67686 to RRJ. TB was supported by fellowships (PBLAP3-123417 and PA00P3-134165) from Switzerland. YC was supported by a fellowship from Taiwan National Science Council (97-2918-I-006-012).

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