PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of ambexSpringerOpen.comThis journalSubmit a manuscriptRegisterSpringerOpen.comAMB Express
 
AMB Express. 2012; 2: 20.
Published online Mar 29, 2012. doi:  10.1186/2191-0855-2-20
PMCID: PMC3350409
Bioconversion of glycerol to ethanol by a mutant Enterobacter aerogenes
RES Nwachukwu,1 A Shahbazi,corresponding author2,5 L Wang,2 S Ibrahim,3 M Worku,4 and K Schimmel1
1Energy and Environmental Systems, North Carolina A & T State University, Greensboro, NC, USA
2Biological Engineering Program, North Carolina A & T State University, Greensboro, NC, USA
3Food and Nutritional Sciences, North Carolina A & T State University, Greensboro, NC, USA
4Department of Animal Sciences, North Carolina A & T State University, Greensboro, NC, USA
5Biological Engineering Program, Sockwell Hall, North Carolina A & T State University, 1601 East Market Street, Greensboro, NC 27411, USA
corresponding authorCorresponding author.
RES Nwachukwu: nwannaray/at/yahoo.com; A Shahbazi: ash/at/ncat.edu; L Wang: lwang/at/ncat.edu; S Ibrahim: ibrah001/at/ncat.edu; M Worku: worku/at/ncat.edu; K Schimmel: schimmel/at/ncat.edu
Received January 21, 2012; Accepted March 29, 2012.
Abstract
The main objective of this research is to develop, by adaptive evolution, mutant strains of Enterobacter aerogenes ATCC 13048 that are capable of withstanding high glycerol concentration as well as resisting ethanol-inhibition. The mutant will be used for high ethanol fermentation from glycerol feedstock. Ethanol production from pure (P-) and recovered (R-) glycerol using the stock was evaluated. A six-tube-subculture-generations method was used for developing the mutant. This involved subculturing the organism six consecutive times in tubes containing the same glycerol and ethanol concentrations at the same culture conditions. Then, the glycerol and/or ethanol concentration was increased and the six subculture generations were repeated. A strain capable of growing in 200 g/L glycerol and 30 g/L ethanol was obtained. The ability of this mutant, vis-à-vis the original strain, in utilizing glycerol in a high glycerol containing medium, with the concomitant ethanol yield, was assessed. Tryptic soy broth without dextrose (TSB) was used as the fermentation medium. Fermentation products were analyzed using HPLC.
In a 20 g/L glycerol TSB, E. aerogenes ATCC 13048 converted 18.5 g/L P-glycerol and 17.8 g/L R-glycerol into 12 and 12.8 g/L ethanol, respectively. In a 50 g/L P-glycerol TSB, it utilized only 15.6 g/L glycerol; but the new strain used up 39 g/L, yielding 20 g/L ethanol after 120 h, an equivalence of 1.02 mol ethanol/mol-glycerol. This is the highest ethanol yield reported from glycerol bioconversion. The result of this P-glycerol fermentation can be duplicated using the R-glycerol from biodiesel production.
Keywords: Enterobacter aerogenes, Ethanol, Glycerol, Biodiesel, Fermentation
Articles from AMB Express are provided here courtesy of
Springer-Verlag