Strong evidence that cortically-localized dynein can mediate interactions between microtubules and the cell periphery comes from studies in S. cerevisiae, where dynein’s primary role is to exert tension on microtubules projecting from the spindle pole body in order to properly position the nucleus at the bud neck [2
]. A parallel mechanism also functions in higher eukaryotes, as cortically-localized dynein has been implicated in the proper positioning of the spindle in dividing cells in C. elegans, Drosophila, and human cells [5
]. In interphase cells as well, cortically-localized dynein mediates microtubule capture and tethering, primarily at sites of cell-cell interaction such as adherens junctions and the immunological synapse [6
]. Here, we explored the mechanistic basis of microtubule tethering by mammalian dynein, using in vitro assays employing optical trapping, reconstitution of microtubule dynamics, and TIRF microscopy.
We first asked if dynein-bound polystyrene beads could tether projecting microtubule plus-ends. We anchored polarity-marked Taxol-stabilized microtubules to a coverslip via their biotinylated seeds, so that the microtubule minus-ends were fixed. This geometry allows the unbiotinylated and thus unattached plus-ends to undergo lateral diffusive movements (). Then, in the presence of 1 mM MgATP, we used an optical trap to bring dynein- or BSA-coated beads, one at a time, to the mobile plus-end of a microtubule. When BSA-coated beads were brought near the microtubule plus end, the variance in the lateral position did not decrease ( and Supplementary Movie S1
). In contrast, when dynein-coated beads were brought near the microtubule plus end, the microtubule became tethered, resulting in a pronounced decrease in the variance in the lateral position of the microtubule tip ( and Supplemental Movie S1
Dynein tethers microtubule plus-ends in vitro
When released from the trap, beads moved towards the minus end, consistent with dynein motor function (Supplemental Fig. S1
). We used the optical trap to measure the force exerted by the dynein-bound beads on microtubules stably attached to the cover slip along their length, and found forces ranging from 2-8 pN (). As mammalian cytoplasmic dynein has a unitary stall force of ~1.1 pN and the force applied by multiple dynein motors is close to additive under these conditions [10
], this suggests that 2-8 dynein motors may simultaneously interact with an individual microtubule in this assay.
Next, we asked whether these effects were unique to dynein, or if other microtubule-associated proteins might have the same effect of dampening the lateral mobility of the microtubule plus-end, consistent with tethering. We independently compared the effects of either purified recombinant kinesin-1 or the plus end-tracking protein EB1 on microtubule mobility using the optical trap assay. Both kinesin-1- and EB1-bound beads were able to bind to the microtubule, decreasing the lateral variance of the microtubule plus-end as compared to BSA-bound control beads. However, neither kinesin-1 nor EB1 decreased the lateral variance of the microtubule as effectively as dynein (). Further, we observed distinct effects of these proteins on the microtubule plus end relative to dynein (Supplemental Movie S1
). While the microtubule remained in contact with the bead, the plus-end continued to “search” the surface of either kinesin-1-bound or EB1-bound beads resulting in less-effective tethering (Supplemental Movie S1
). In the case of kinesin-1, we occasionally observed microtubule buckling, consistent with the application of a compressive load on the microtubule by a plus end-directed motor. This was not seen with EB1-bound beads, where the microtubule plus end remained in contact with the bead but continued to search the surface (Supplemental Movie S1
), likely due to individual binding and release events from multiple EB1 molecules bound to the bead surface. In contrast, dynein-bound beads induced a more stable attachment with the microtubule end, exerting tension that appeared to stiffen the microtubule, decreasing lateral fluctuations along its length (Supplemental Movie S1
Microtubules in the cell are dynamic, undergoing periods of growth and shrinkage known as dynamic instability due to the addition and loss of tubulin subunits, primarily at their plus ends [12
]. These dynamics allow the plus-ends to sample the cytoplasm over time, exploring cellular space. We next asked whether dynein-bound beads could tether dynamic microtubule plus ends (). In a flow chamber maintained at 37 °C, we bound biotinylated microtubule seeds and BSA- or dynein-coated beads to the coverglass surface. We next added 10 μM tubulin dimers to the chamber, reconstituting dynamic instability. Microtubule dynamics were monitored by total internal reflection fluorescence (TIRF) microscopy.
Dynein-mediated tethering of dynamic microtubule plus-ends is ATP-dependent
Interactions of dynamic microtubules with BSA-coated beads often led to rapid catastrophe in this assay ( and Supplemental Movie S2
), consistent with previous observations demonstrating induction of catastrophe by contact with a rigid barrier [13
]. In contrast, microtubules that encounter dynein-bound beads in the presence of ATP did not often rapidly catastrophe. Instead, the initial encounter of the microtubule with the bead was stabilized. In some encounters with dynein-bound beads, the microtubule remained tethered (, left panels). In other encounters, the microtubule plus-end continued to polymerize past the bead while bead-bound dynein maintained a lateral interaction with the microtubule lattice (, right panels and see Supplemental Movie S3
). Importantly, when a growing microtubule plus-end encountered a dynein-bound bead in the presence of 1 mM ATP, we observed a marked decrease in the variance of lateral position consistent with dynein-mediated tethering (), similar to the dynein-mediated tethering observed in the optical trap assays ().
To determine the effects of dynein-mediated tethering on microtubule stability, we measured the time from initial contact to catastrophe for dynein-coated beads as compared to control beads. We found that in the presence of ATP, the mean time from contact to catastrophe was 161±25 s for dynein-bound beads, as compared to 67±13 s for BSA-coated beads ( and S2
, P<0.02). Only microtubules making direct contact with beads were affected, as no significant differences were detected in microtubule dynamics away from the beads under any of the conditions tested ().
Strikingly, although dynein is known to bind tightly to microtubules in the absence of ATP (apo state), encounters between microtubule ends and dynein-coated beads in the absence of ATP were not significantly different than microtubule ends encountering BSA-coated beads (, P>0.7). Thus, the ATP-dependent motor activity of dynein, and not just the high affinity binding of dynein to microtubules, is required to stabilize interactions of dynamic microtubules with a barrier.
To further probe the tethering mechanism of dynein, we adapted a previously described three-dimensional computational model that explicitly considers the forces within the microtubule lattice exerted through lateral and longitudinal bonds between each tubulin subunit [14
] (). In this model, the nucleotide state of each tubulin subunit determines its preferred angle in the microtubule lattice: GTP-bound tubulin subunits form straight protofilaments while protofilaments with GDP-tubulin subunits tend to curl outwardly when exposed [16
], destabilizing the polymer and encouraging catastrophe (). We hypothesized that minus-end directed dynein can stabilize microtubule plus-ends by exerting tension to straighten curled microtubule protofilaments. The force required to straighten a single protofilament is ~1.25 pN [17
], similar to the unitary stall force of mammalian dynein (~1.1 pN; [11
]). We simulated this situation by allowing microtubule tips to grow against a barrier (), and then randomly selected individual protofilaments to be straightened by the action of dynein pulling near the plus-end. We found that the time to undergo a catastrophe event was prolonged when dynein applied force to straighten individual protofilaments (). The length of time that a microtubule plus-end was stabilized (time to catastrophe) was found to increase as a function of the number of protofilaments that were acted on by dynein ().
Cortically-localized dynein tethers and stabilizes microtubules
These simulations predict that minus-end directed dynein motors that are anchored at the cell cortex can transiently stabilize microtubule plus ends and delay catastrophe events by straightening individual protofilaments, resulting in dampened microtubule dynamics. Interestingly, the model predicts the number of engaged dynein motors required to stabilize microtubules is similar to the number of engaged motors estimated in our in vitro optical trap assays. Thus, even substoichiometric levels of dynein (1-4 molecules interacting with a 13-protofilament microtubule) would be expected to appreciably suppress catastrophe by counteracting the initial deforming force exerted on the microtubule as it encounters an organelle or the plasma membrane. Both the data and simulations predict that this stabilization requires active force production - high affinity binding alone would not be sufficient for this sort of microtubule capture, i.e. where catastrophe is delayed.
Our observations are consistent with previous observations that encounters between a growing microtubule plus end and a rigid barrier induce catastrophe [13
]. However, we show that when dynein is present, the microtubule plus end is stabilized, allowing the microtubule to continue to polymerize past the bead barrier. This stabilization requires dynein motor activity, as the observed stabilization is ATP-dependent. Thus, active generation of tension is required. Computational modelling suggests that dynein stabilizes the dynamic microtubule plus end through exertion of tension to straighten individual protofilaments. Also, active dynein motors may continuously remodel the connection between the microtubule and barrier, allowing the productive formation of a lateral contact as the microtubule grows past the bead.
The mechanochemistry of cytoplasmic dynein may be uniquely suited for a role in microtubule tethering. Mammalian cytoplasmic dynein has a relatively low stall force [11
] compared to other motors such as kinesin-1, and a variable stepping pattern along the microtubule that includes both sideways and backward steps [21
]. The nature of the tethering may also be affected by the number of dynein motors acting on a microtubule plus end. Both our trapping assays and our simulations predict that substoichiometric levels of dynein (1-4 molecules interacting with a 13-protofilament microtubule) would suppress catastrophe by counteracting the initial deforming force exerted on the microtubule as it encounters the plasma membrane. However, either the activation of cortical dynein [23
] or the recruitment of additional dynein motors to the cortex may lead to modulation of microtubule plus-end dynamics at the cell cortex.
Importantly, these results indicate that dynein not only captures and tethers dynamic microtubule plus ends, but can also stabilize these ends and thus modulate microtubule dynamics. This influence on microtubule dynamics may promote the stabilization of specific microtubules, which could serve as preferred tracks for intracellular transport between cell center and subdomains at the cell periphery. As both motor and tether, dynein can also exert force on the cytoskeleton relative to the cortex. This mechanism is critical to spindle positioning in a number of systems [2
], as dynein can mediate the association of astral microtubules with the cell cortex in dividing cells. However, dynein has also been localized to the cell cortex at sites of cell-cell adhesion [6
]. Microtubule tethering at these sites may facilitate a connection between the dynamic microtubule cytoskeleton and intercellular adhesion molecules, creating a powerful mechanism for cells to react to stimuli in the extracellular environment (). However, further studies to assess the role of dynein in mediating intercellular interactions will be necessary to determine whether the critical function of dynein at these sites is facilitated trafficking or the transduction of force.