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Objectives. The objective of this paper is to determine if hyaluronan affects bupivacaine's anesthetic function. Methods. Whole cell patch clamp recordings were performed on bovine articular chondrocytes cultured in 60mm dishes. The chondrocytes were treated with phosphate-buffered saline (control group), 7.5mg/mL hyaluronan (Orthovisc), 0.25% bupivacaine, or a mixture of 7.5mg/mL hyaluronan and 0.25% bupivacaine. Outward currents were elicited by step depolarization from −90mV to 150mV with 5mV increments and holding for 200ms. Results. The amplitude of outward currents elicited at 150mV was 607.1 ± 135.4pA (mean ± standard error) in the chondrocytes treated with phosphate buffered saline, 550.0 ± 194.9pA in the chondrocytes treated with hyaluronan, 18.4 ± 8.3pA in the chondrocytes treated with bupivacaine, and 12.8 ± 2.6pA in the chondrocytes treated with a mixture of hyaluronan and bupivacaine. Conclusion. Hyaluronan does not affect bupivacaine's inhibitory action on the potassium channel activities in bovine articular chondrocytes. This finding suggests that intra-articular injection of a mixture of hyaluronan and bupivacaine may not affect the anesthetic effects of bupivacaine.