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BMC Infect Dis. 2012; 12(Suppl 1): P21.
Published online May 4, 2012. doi:  10.1186/1471-2334-12-S1-P21
PMCID: PMC3344762
Incidence of bla genes among uropathogenic Escherichia coli isolates from HIV and non-HIV patients in South India
Kesavaram Padmavathy,corresponding author1,2 Padma Krishnan,1 and Sikhamani Rajasekaran3
1Dept of Microbiology, Dr. ALM PGIBMS, University of Madras, Chennai, India
2Sree Balaji Dental College and Hospital, Chennai, India
3Government Hospital of Thoracic Medicine, Chennai, India
corresponding authorCorresponding author.
Kesavaram Padmavathy: padmabakianath/at/yahoo.co.in
Supplement
Abstracts from the First International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2012)
Suniti Solomon, Sunil S Solomon, Pachamuthu Balakrishnan, Kailapuri G Murugavel, Shanmugam Saravanan, Hussain S Iqbal and Ramachandran Vignesh
Publication of this supplement has been supported by the NIH/Office of AIDS Research, ICMR, DBT and CSIR.
1471-2334-12-S1.pdf
Conference
First International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2012)
20-22 January 2012
Chennai, India
Background
Group 3a/b cephalosporins are currently being used in the treatment of UTI and urosepsis. However, Extended Spectrum Beta-Lactamase (ESBL) mediated resistance has been increasingly reported among uropathogens from HIV patients. We sought to determine the incidence of ESBL genes- blaCTX-M, blaTEM and blaSHV among E. coli isolates from HIV (with increased exposure to cephalosporins) and non-HIV antenatal patients.
Methods
PCR detection of blaCTX-M, blaTEM and blaSHV were carried out among ESBL producing urinary E. coli isolates from HIV (n=57) and non-HIV antenatal patients (n=22). Fisher’s exact test was employed to analyze the statistical significance of the results.
Results
Overall, 31.7%, 59.5% of the E. coli isolates carried blaTEM, blaCTX-M respectively, while none harboured blaSHV. When stratified based on host group, significant difference was observed in the incidence of blaCTX-M among the isolates from HIV and non-HIV patients (70.2% vs 31.8% respectively, p = 0.0024; OR 5.042; 95% CI = 1.7441-14.5759). Nonetheless, difference in prevalence of blaTEM among the HIV and non-HIV isolates was not statistically significant (29.8% vs 36.4%, p = 0.5979). Co-occurrence of blaTEM and blaCTX-M was detected among 22.8%, 0% of the E. coli isolates from HIV and non-HIV patients respectively (OR 5.1447; 95% CI = 1.3766-19.2273).
Conclusion
Our results augment the fact that frequent exposure to cephalosporins serves as the driving selection force leading to increased incidence of ESBL (blaCTX-M) mediated resistance among the E. coli isolates from HIV patients. Hence, the risk associated with antimicrobial exposure needs to be considered in therapeutic decision making.
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