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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Pediatr. Author manuscript; available in PMC 2013 June 1.
Published in final edited form as:
PMCID: PMC3340437
NIHMSID: NIHMS350224

β-cell dysfunction in Adolescents and Adults with Newly Diagnosed Type 2 Diabetes

Abstract

Objective

To compare β-cell function in adolescents and adults with newly diagnosed type 2 diabetes (T2DM).

Study design

Thirty-nine adolescents with T2DM, 38 age- and weight matched controls, and 19 adults with T2DM were studied. The adolescent diabetic subjects were divided based on whether they needed insulin to control their initial hyperglycemia. The primary outcome variable was the Disposition Index (DI), computed from the acute insulin response to glucose (AIRg) corrected for insulin sensitivity (1/HOMA-IR).

Results

DI was significantly reduced in all 3 diabetic groups (Control n=3360, adolescents with T2DM without insulin n=630, adolescents with T2DM with insulin n=120, adults with T2DM n=200; p < 0.001), and the adolescents with more severe hyperglycemia at diagnosis had lower DI than those with a more modest presentation (p < 0.05).

Conclusion

At the time of diagnosis, adolescents with T2DM have significant β-cell dysfunction, comparable with newly diagnosed adults. Thus, severe β-cell impairment can develop within the first two decades of life and is likely to play a central role in the pathogenesis of T2DM in adolescents.

Keywords: adolescent T2DM, obesity, insulin secretion

The incidence of type 2 diabetes mellitus (T2DM) in adolescents has increased significantly over the past 2 decades, coincident with increased rates of obesity (15). The pathophysiology and natural history of T2DM in adolescents is unclear and similarities to the pathogenesis of diabetes in adults have been assumed but not proven. Cross sectional evaluations of adolescents with established T2DM have demonstrated that nearly all have obesity and severe insulin resistance (68). In addition, impaired insulin secretion in response to oral and intravenous glucose is also typical (9, 10). Although some of the abnormalities of insulin secretion reported in adolescents with T2DM of several years duration are similar to those reported in diabetic adults (68, 11), β-cell function in adolescents with recently-diagnosed T2DM has not been characterized.

Functional defects in insulin secretion are central to the development of T2DM in adults, and current evidence suggests that the capacity for insulin secretion declines gradually in the years before presentation, progressing eventually to a loss of normal glucose homeostasis (1215). Adult subjects at risk for developing T2DM have detectable abnormalities of β-cell function, even though regulation of blood glucose remains normal (16, 17), suggesting either a constitutive islet cell defect or a process with a long evolution. Although the actual course of β-cell decline from normal glucose tolerance to T2DM has only been estimated (18), longitudinal studies in specific, high-risk subjects indicate that loss of insulin secretion sufficient for progression from normal glucose tolerance to diabetes occurs over a period of 4–6 years (19). Part of the loss of insulin secretory capacity may be due to a reduction in the number of β-cells because autopsy studies of adults with diabetes suggest a greater than 50% reduction in β-cell mass compared with normoglycemic adults (15). However, a reduction in the functional capacity of β cells is also likely to contribute to the onset of diabetes (20).

The pathogenic model of T2DM in adults, with gradual decline in β-cell function and islet mass over years, is difficult to extrapolate to onset of disease in adolescence. In young people at risk for developing T2DM, the pre-morbid state is necessarily shorter and suggests a different rate of disease progression or a pathogenesis distinct from adult diabetes. One possibility is that β-cell dysfunction is not as severe in adolescents at the time they present with clinical disease. Several groups, including our own, have noted preservation of the acute insulin response to glucose (AIRg) in pediatric T2DM subjects, although this measure is impaired relative to the degree of insulin resistance (6, 8). By contrast, in adults with T2DM the AIRg is impaired even prior to the onset of diabetic hyperglycemia (13). One interpretation of these findings is that there is greater retention of β-cell capacity early in the course of T2DM in adolescents, consistent with a shorter period for disease development in younger patients. However, this conclusion is based only on indirect comparisons of β-cell impairment between adolescents and adults made across separate studies.

We hypothesized that compared with adults with new onset T2DM, adolescents with diabetes would have greater preservation of β-cell function. To test this hypothesis we measured β-cell responses to intravenous (IV) glucose and arginine in groups of adolescents at the time of presentation with T2DM, age- and weight-matched nondiabetic controls, and a group of recently diagnosed T2DM adults. The results of this study show that shortly after diagnosis, adolescents with T2DM have severe β-cell dysfunction that is comparable with the deficits seen in adult diabetes.

Methods

Thirty-nine adolescents newly diagnosed with T2DM (within 2.2 ± 0.6 mos), and 41 nondiabetic obese subjects of similar age and weight (controls) were recruited from Cincinnati Children’s Hospital Medical Center Hospital (CCHMC). Individuals with active concurrent illnesses, secondary diabetes, or pregnancy were excluded from the study. Three adolescent controls that subsequently developed T2DM in a longitudinal follow-up of the study were excluded from the baseline analysis to avoid misclassification, resulting in 38 adolescent controls analyzed here. Nineteen adults with new-onset T2DM (Adult T2DM) were recruited from the Greater Cincinnati Area by newspaper advertisement. These subjects were within 6.5 ± 3.0 months of diagnosis and so were slightly later in their course than the diabetic adolescents. The different recruitment strategies reflect differences in diabetes management in our region, where children and adolescents are referred promptly to our Children’s Hospital for treatment and education, and adults are diagnosed and treated by primary care physicians as outpatients.

At the time of diagnosis the adolescents with diabetes met American Diabetes Association criteria for diagnosis of T2DM (21). Twenty-two of these subjects (56%) had been treated with insulin in the weeks before being studied to reverse uncontrolled hyperglycemia, but none had received insulin for at least 36 hours before testing. The decision to use insulin was the judgment of the clinician directing initial management of the patients and not based on standardized criteria. Nonetheless, this distinction in treatment separated the adolescent T2DM group by severity at onset and was used to subdivide the cohort after recruitment was completed but prior to analysis of outcomes: adolescents with T2DM initially receiving insulin (n=22) and adolescent with T2DM not requiring insulin (n=17). None of the adolescents with T2DM had a presentation consistent with ketoacidosis, none had markers of islet cell autoimmunity, using conventional clinical screening for glutamic acid decarboxylase-65, islet cell antibody 512 and insulin auto-antibody, and all had acanthosis nigricans at diagnosis. All adolescent subjects with T2DM were pubertal (Tanner IV or greater) based on examination by a pediatric endocrinologist. Of the adult diabetic subjects (32%) were managed with diet alone, and the remainder with oral anti-diabetes medications; none was receiving insulin. All diabetic subjects were asked to withhold their oral diabetes medications for 3 days prior to study visits.

The obese control subjects were recruited from an obesity research program at CCHMC and had normal glucose tolerance as measured by a 75 g OGTT; thirteen percent (5/38) were taking Metformin for management of insulin resistance. All the subjects started on metformin had documentation of normal glucose tolerance before starting treatment; metformin was discontinued for 3 days before β-cell function testing.

The study was approved by the CCHMC and University of Cincinnati Institutional Review Boards, and all subjects (or their parents, for those < 18 years old) provided written informed consent or with written assent as appropriate (for patients < 18 years old) to participate.

β-cell function tests

Consenting subjects reported to the General Clinical Research Center at CCHMC after an overnight fast for evaluation. Indwelling venous catheters were placed in both forearms for infusion of test substances and blood sampling; the arm designated for blood drawing was wrapped in a heating pad to arterialize venous blood (22). Subjects had sequential bolus administration of arginine (5 grams) and glucose (0.3 grams/kg, max dose 40 grams) with 50 minutes between infusions to allow equilibration of plasma glucose and islet hormones. Blood was sampled over 15 minutes following arginine and for 30 minutes after glucose to determine insulin responses. All blood samples were immediately placed on ice and centrifuged to separate plasma within an hour of collection. Plasma was separated and stored at −80°C until processing.

Biochemical measurements

Plasma glucose measurements were made on whole blood using a YSI glucose analyzer (Yellow Springs Instrument, Yellow Springs, OH). Plasma insulin was measured by radioimmunoassay (RIA) as previously described (8). This assay uses a guinea pig anti-insulin serum that does not distinguish between proinsulin and fully processed insulin. C-peptide and proinsulin were measured using commercial RIA kits (Millipore/Linco, Inc., St. Louis, MO). Hemoglobin A1c (HbA1c) was measured using a modification of a high-performance-liquid-chromatography method using an Alliance 2690/2695 HPLC (Waters Corporation, Milford, MA) and a PolyCAT A column (PolyLC, Inc Columbia, MD) to separate the hemoglobin fractions by cation-exchange chromatography; HbA1c results are reported as percent of total hemoglobin.

Calculations and Statistical Analysis

Fasting glucose and insulin levels were calculated as the mean of 3 values before arginine administration; baseline levels for the IV glucose portion of the study were the mean of two samples taken in the 10 minutes before infusion. The acute insulin responses to arginine (AIRARG) and glucose (AIRg) were computed as the area under the curve over baseline of insulin concentrations at 2, 4, 6, 8, and 10 minutes after stimulus administration using Riemann sums based on the midpoint of each interval. The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the online Oxford calculator (www.dtu.ox.ac.uk/homa). The disposition index (DI) was defined as the product of insulin secretion (AIRg) and insulin sensitivity (1/HOMA-IR). The glucose disappearance constant (Kg) was calculated as the slope of the natural logarithm of blood glucose concentrations from 10–30 minutes after glucose infusion and is expressed as percent change per minute. Fasting proinsulin to insulin ratios (PI/I) were calculated for each subject before the glucose bolus by dividing the mean of two proinsulin measurements by the mean of two insulin measurements. Stimulated PI/I ratios were calculated in a similar manner using two post-glucose infusion measurements of proinsulin and insulin.

All analysis were conducted considering four study groups, dividing the adolescent T2DM group based on initial management (e.g. insulin vs. no insulin), as described above. The decision to subdivide this group was made prior to analysis of outcomes. Data were analyzed using SAS v. 9.2 (SAS Institute, Cary, NC). Continuous baseline data were summarized as mean ± standard error of the mean (SEM), and differences in baseline characteristics among groups were analyzed using ANOVA with Tukey-Kramer adjustments for multiple pairwise comparisons between groups. Categorical data were summarized as frequency counts with percentages, and differences between groups were analyzed using χ2 or Fisher’s Exact Test, as appropriate.

Primary (DI) and secondary (AIRg, AIRARG) outcomes were skewed, so analysis of metabolic testing outcomes were conducted using nonparametric methods. Comparisons of median values among the four groups were made using the Kruskal-Wallis test, with pairwise multiple comparisons controlled for using Tukey-Kramer adjustments. Potential differences in primary and secondary outcomes by metformin usage, race and sex were carefully considered, given the imbalance in these factors at enrollment. Sensitivity analysis revealed no significant effect of any of these factors (metformin, race, or sex) on any of the main outcomes within each study group (all adjusted p-values ≥ 0.05), indicating that imbalance in the prevalence of these factors across groups did not have a major impact on the reported group differences. Therefore, unadjusted comparisons among groups are presented. P-values <0.05 were considered significant.

Results

Table I presents the baseline characteristics of the 4 study groups. Across all groups BMI was comparable, and family history of diabetes did not differ. The adolescent diabetic and control groups were age-matched by design. There were no differences in Tanner staging among the adolescent groups. Adults with T2DM had a greater proportion of non-Hispanic white (NHW) participants than any of the adolescent groups, and was relatively over-represented by women. Adults with T2DM were enrolled later after diagnosis than either adolescent T2DM group.

Table 1
Clinical and demographic characteristics of the study groups

Basal Metabolic Profile

At the time of metabolic testing all 3 diabetic groups had moderate fasting hyperglycemia compared with the adolescent control group (Table II). The adolescents with T2DM receiving insulin also had a significantly higher HbA1c than either of the other groups with T2DM, likely reflecting the glycemic severity at presentation. Adults with T2DM had lower fasting insulin and lower HOMA-IR than any of the adolescent groups, with adolescent controls exhibiting intermediate HOMA-IR values. Fasting proinsulin to insulin ratios (PI/I) were highest in adults with T2DM and lowest in adolescent controls and adolescents with T2DM receiving insulin; adolescents with T2DM not receiving insulin were intermediate.

Table 2
Results from metabolic testing

Stimulated β-cell function

Starting from basal glucose levels at time 0, the diabetic subjects had higher circulating glucose concentrations than the controls at each step after the arginine (Mean0–10 min: adolescent controls 89.5 ± 1.0, adolescents with T2DM not receiving insulin 118.2 ± 7.5, adolescents with T2DM receiving insulin 127.2 ± 8.6, adults with T2DM 149.3 ± 13.3 mg/dl) and glucose boluses (Mean2–10 min: adolescent controls 258.1 ± 5.6, adolescents with T2DM not receiving insulin 284.1 ± 9.2, adolescents with T2DM receiving insulin 290.3 ± 10.2, adults with T2DM 322.8 ± 21.5 mg/dl). However, the increment in glucose above basal was not different among the groups (Table II).

AIRARG was significantly reduced in adolescents with T2DM receiving insulin and adults with T2DM patients compared with adolescent control group (p<0.001; Table II). AIRg was significantly reduced in all groups with T2DM compared with adolescent controls (Figure, A and Table II). Furthermore, adolescents with T2DM not receiving insulin had higher AIRg than adults with T2DM after glucose stimulation. Adolescents with T2DM receiving insulin exhibited AIRg values that were not significantly different from either of the other groups with T2DM. When AIRg was corrected for insulin sensitivity, as the disposition index (DI), the β-cell responses to IV glucose in the three diabetic groups were significantly lower than the adolescent controls, but with adolescents with T2DM not receiving insulin were significantly higher than adolescents with T2DM receiving insulin (Figure, B and Table II).

Figure
A, AIRg and B, DI values for all subjects for each group. Open circles are drawn for each data point. Line within box is the median. Diamond is the mean. Top of box is the 75th percentile; bottom of box is the 25th percentile. Whiskers in box plots encompass ...

Clearance of glucose after the glucose infusion (glucose disappearance constants (Kg) was significantly and similarly diminished in all of the groups with T2DM, relative to adolescent controls (Table II). Also, after glucose stimulation all of the groups with T2DM had significantly higher PI/I ratios than adolescent controls.

Discussion

We directly compared insulin secretion in adolescents and adults with newly diagnosed T2DM. The results demonstrate that adolescents with T2DM have a marked impairment of insulin secretion at the time of diagnosis, even after a short course of treatment to correct hyperglycemia. Moreover, within our cohort of adolescent diabetic subjects there was a wide range of insulin secretory capacity and those with the most severe hyperglycemia at presentation, prompting insulin treatment at diagnosis, were more severely affected than those with more modest elevations of blood glucose. These findings do not support our hypothesis that adolescents with new onset T2DM have relatively greater preservation of β-cell function than adults newly diagnosed. In fact, our findings indicate that severe β-cell impairment is comparable with that of recently diagnosed adults with T2DM and a central feature of the presentation of T2DM in adolescence.

Defining new onset T2DM is inexact given the frequency of asymptomatic preclinical disease, and our cohort likely includes persons with a range of diabetes duration and severity. To arrive at a group representative of early T2DM, we recruited adult subjects within 1 year of diagnosis who were managed with diet or oral agents. Based on fasting glucose, HbA1c and diabetes treatment, this group was generally comparable with the one-half of our adolescent with diabetes not treated with insulin. Because the course of development of pediatric T2DM is unknown (23) defining adult and teenage cohorts at a comparable stage of disease involves some uncertainty. However, the limited evidence that does exist suggests that T2DM develops over years in adolescents as well as adults (24, 25). Thus, using recent diagnosis as the basis for aligning the adolescent and adult subjects for comparison is the most straight forward criteria now available and insures that all the subjects met common criteria for diabetes.

In the current study, the adolescents with T2DM are typical of the increasing population of adolescents with T2DM seen in our clinic (26). The majority of newly diagnosed adolescents in our region are admitted to the hospital for acute metabolic stabilization and education. Thus, most of the adolescents with T2DM were recruited as inpatients but studied 1–2 weeks after glucose stabilization when they returned for further diabetes education. Despite common ascertainment and initial stabilization, there was heterogeneity among this group of diabetic adolescents. One critical distinction was that approximately half were deemed to have severity of disease that merited insulin treatment from the outset; these subjects had a mean HbA1c of 8.9 ± 0.6%. In those subjects in whom a clinical decision was made not to use insulin, but to start Metformin instead, the baseline HbA1c was 6.5 ± 0.2%. Interestingly these two groups did not differ in age, race, BMI, or HOMA-IR, but had clearly distinct measures of insulin secretion. β-cell function was worse in the insulin treated subjects, even after a week or more of treatment for hyperglycemia, consistent with other reports of adults and adolescents in which diabetic subjects with more dramatic presentations had evidence of worse insulin secretion (27). The distinct presentations in our adolescent diabetic cohort suggest significant differences in either the stage of disease or rate of β-cell failure among teenagers developing this condition.

The adolescents with T2DM had insulin secretion that was generally similar to the adult diabetic subjects. Adolescents with T2DM receiving insulin had similarly low AIRARG as the adults with T2DM, and adolescents with T2DM without insulin had AIRARG comparable with the controls. However, AIRg was significantly reduced in all the diabetic groups and this was especially apparent when corrected for insulin resistance as DI. Thus, although there was a range of β-cell function among the adolescent diabetic subjects that could be dichotomized based on presentation, overall these findings indicate that insulin secretion in adolescents with new onset T2DM is qualitatively and quantitatively similar to adults with early diabetes. This is somewhat surprising because the adults with T2DM almost certainly had a longer prediabetic period, and thus more time to develop β-cell dysfunction (27).

An increased percentage of plasma proinsulin is an established marker of β-cell dysfunction in adults with T2DM (28). In our groups of diabetic and nondiabetic subjects the adults had higher PI/I in fasting plasma than adolescent controls and adolescents with T2DM on insulin, with the adolescents with T2DM not on insulin intermediary between the other groups. This is similar to some (6, 11) but not all (7) previous measures of these β-cell products in adolescent T2DM. However, following stimulation with IV glucose there was a higher proportion of proinsulin secreted among the diabetic compared with subjects without diabetes. Even though adults with T2DM tended to have the highest percentage of proinsulin in the β-cell response to glucose stimulation, this measure clearly separated the subjects with abnormal from those with normal insulin secretion, as has been consistently reported in previous studies of older subjects with diabetes (28).

There is little data currently available on the natural history of β-cell function in adolescent T2DM with which to estimate the time course for β-cell decline in adolescents. In one report there was a one year reduction in DI of ~ 30% in pubertal Hispanic teens at high risk for T2DM (29). Cali et al published findings from a longitudinal study of obese young people with normal glucose tolerance (24). During 2–3 years of follow-up, 23% of this cohort developed impaired glucose tolerance, coincident with a substantial reduction of insulin sensitivity. However, the subjects progressing to impaired glucose tolerance had relatively lower insulin responses at baseline and a decrease in β-cell function over time as reflected in a reduction of DI by ~ 20% per year. Extrapolating from these estimated rates of DI decline, we speculate that β-cell failure in our 15 year old subjects likely began before age 12. This time frame is consistent with a case report of a high-risk 10 year old girl progressing from insulin resistant but normal glucose tolerant to T2DM over 5 years (25).

There are several limitations to this study that must be considered in the interpretation of the results. First, even though the diabetic groups were representative of patients with early T2DM seen in our adolescent and adult diabetes clinics, the sample sizes are small, and demographically and clinically diverse, limiting our ability to identify specific factors that could contribute to our findings. In particular the imbalance of sex and race between the adolescent and adult groups has the potential for confounding. It is clear that in both adults and pediatric populations, African-Americans have higher insulin secretion than NHW individuals (3033), and a similar but less pronounced effect has been described for female sex (34). We recognize that other studies have noted differences in insulin secretion among the races, and our study may have detected differences if the sample size was larger. However, within our patient groups, the effects of race and sex were not significant, and analysis of the unadjusted numbers provides conservative estimates of between group differences such that we think it is unlikely that these variables affected our final conclusions in a meaningful way. Another variable that could have confounded the results were differences in the use of metformin. In the 5 metformin treated controls AIRg and DI were reduced relative to the others in this group, but metformin treatment had no effect on β-cell function within the diabetic groups. Therefore we also think it is unlikely that variable metformin use among the subjects affected the outcome of this study.

A second limitation is the potential influence of chronic hyperglycemia on β-cell function in the adolescents with T2DM. These subjects generally came to clinical attention based on symptoms of hyperglycemia, and 24 of these youths presented with blood glucose levels > 20 mmol/l. However, all our adolescents with T2DM were treated immediately and not studied until 1–2 weeks later. Previous studies have demonstrated that even several days of insulin therapy is sufficient to reverse glucose toxicity and improve insulin secretion (35, 36). That this occurred in our subjects is suggested by the only moderate fasting hyperglycemia (6.7 ± 0.3 mmol/l) that was present at the time of formal evaluation. A third limitation was the difference in ascertaining our adult and adolescent diabetic groups. Most adolescents in our area discovered to be hyperglycemic are referred promptly to our medical center for treatment, coming to immediate attention for treatment and education. Adults diagnosed with T2DM are treated less urgently and were recruited by advertisement. The different methods of recruitment may have enriched our adult cohort with less severely affected subjects. We attempted to mitigate this possibility by subdividing our adolescent diabetic cohort by severity of hyperglycemia at diagnosis. Our results demonstrate that the adults more closely resemble the more severely affected adolescents with T2DM, although it is important to note that the adolescents with T2DM did not differ from each other by any other characteristic. Finally, we used only short-term measures of the acute insulin response, and did not directly measure insulin sensitivity, but rather used the HOMA-IR estimate. We chose a shorter, less intense, protocol for β-cell function testing rather than traditional methods like the hyperglycemic clamp out of concern for the convenience of subjects just diagnosed with diabetes. The HOMA-IR estimate has recently been validated in adults with T2DM (37, 38), and even though lacking the precision of clamp and IVGTT techniques, is likely to be consistent across our groups of subjects.

In summary, adolescents with new onset T2DM have impaired insulin secretion in response to glucose and arginine, comparable with or worse than that of adults recently diagnosed with diabetes. We cannot determine from this cross sectional examination whether abnormalities of insulin secretion in adolescent diabetics are long-standing, with slow progression similar to the model for adult T2DM, or whether they develop more rapidly in the years to months before clinical presentation. The findings presented here demonstrate that adolescents with T2DM share key defects of insulin secretion with diabetic adults, although more research is necessary to determine whether the pathogenesis and natural history of β-cell dysfunction is shared across the age spectrum.

ACKNOWLEDGMENTS

We thank Brianne Paxton, Kay Ellis and Clinton Elfers for their careful measurement of plasma hormones. We thank the nurses for their expertise with the protocols and their care of the research subjects, and are grateful to our study subjects for their participation.

Funded by National Center for Research Resources (NIH 5K23DK070775-03 to D.E. and R01DK57900 to D.D.), M01 RR 08084, and USPHS (grant #UL1 RR026314).

Abbreviations

T2DM
Type 2 Diabetes Mellitus
HOMA-IR
Homeostatic model assessment of insulin resistance
PI/I
Proinsulin to Insulin ratio
AIRg
Acute insulin response to glucose
AIRARG
Acute insulin response to arginine
BMI
Body mass index
Kg
glucose disappearance constant
HbA1c
Glycosylated hemoglobin percentage

Footnotes

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The authors declare no conflicts of interest.

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