Search tips
Search criteria 


Logo of narLink to Publisher's site
Nucleic Acids Res. 1992 July 25; 20(14): 3551–3554.
PMCID: PMC334000

Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.


Two thermostable DNA polymerases with proofreading activity--Vent DNA polymerase and Pfu DNA polymerase--have attracted recent attention, mainly because of their enhanced fidelities during amplification of DNA sequences by the polymerase chain reaction. A severe disadvantage for their practical application, however, results from the observation that due to their 3' to 5' exonuclease activities these enzymes degrade the oligodeoxynucleotides serving as primers for the DNA synthesis. It is demonstrated that this exonucleolytic attack on the primer molecules can be efficiently prevented by the introduction of single phosphorothioate bonds at their 3' termini. This strategy, which can be easily accomplished using routine DNA synthesis methodology, may open the way to a widespread use of these novel enzymes in the polymerase chain reaction.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (823K), or click on a page image below to browse page by page.

Images in this article

Click on the image to see a larger version.

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press