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Anat Res Int. 2012; 2012: 206238.
Published online Nov 3, 2011. doi:  10.1155/2012/206238
PMCID: PMC3335623
The OPFOS Microscopy Family: High-Resolution Optical Sectioning of Biomedical Specimens
Jan A. N. Buytaert, 1 * Emilie Descamps, 2 Dominique Adriaens, 2 and Joris J. J. Dirckx 1
1Laboratory of Biomedical Physics, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
2Evolutionary Morphology of Vertebrates, Ghent University, K. L. Ledeganckstraat 35, 9000 Gent, Belgium
*Jan A. N. Buytaert: jan.buytaert/at/ua.ac.be
Academic Editor: Tuncay Peker
Received April 28, 2011; Accepted August 12, 2011.
Abstract
We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera. In this paper, the first implementation of LSFM to image biomedical tissue in three dimensions—orthogonal-plane fluorescence optical sectioning microscopy (OPFOS)—is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.
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