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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Ann Rheum Dis. Author manuscript; available in PMC May 1, 2012.
Published in final edited form as:
PMCID: PMC3335437
NIHMSID: NIHMS370935
Impact of IL-1 signalling on experimental uveitis and arthritis
Stephen R Planck,1,2,3 April Woods,1 Jenna S Clowers,1,4 Martin J Nicklin,5 James T Rosenbaum,1,2,3 and Holly L Rosenzweig1,4,6
1Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, USA
2Department of Cell and Developmental Biology, Oregon Health & Science University, Portland, Oregon, USA
3Department of Medicine, Oregon Health & Science University, Portland, Oregon, USA
4Department of Rheumatology, VA Medical Center, Portland, Oregon, USA
5Division of Genomic Medicine, University of Sheffield, Sheffield, UK
6Department of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, Oregon, USA
Correspondence to Holly L Rosenzweig, Casey Eye Institute, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA; rosenzwh/at/ohsu.edu
Abstract
Background
Uveitis, or inflammatory eye disease, is a common extra-articular manifestation of many systemic autoinflammatory diseases involving the joints. Anakinra (recombinant interleukin (IL)-1 receptor antagonist (Ra)) is an effective therapy in several arthritic diseases; yet, few studies have investigated the extent to which IL-1 signalling or IL-1Ra influences the onset and/or severity of uveitis.
Objective
To seek possible links between arthritis and uveitis pathogenesis related to IL-1 signalling.
Methods
The eyes of IL-1Ra-deficient BALB/c mice were monitored histologically and by intravital videomicroscopy to determine if uveitis developed along with the expected spontaneous arthritis in ankles and knees. Expression levels of IL-1R and its negative regulators (IL-1Ra, IL-1RII, IL-1RAcP and single Ig IL-1R-related molecule) in eye and joint tissues were compared. Differences in uveitis induced by intraocular injection of lipopolysaccharide (LPS) in mice lacking IL-1R or IL-1Ra were assessed.
Results
Deficiency in IL-1Ra predisposes to spontaneous arthritis, which is exacerbated by previous systemic LPS exposure. The eye, however, does not develop inflammatory disease despite the progressive arthritis or LPS exposure. Organ-specific expression patterns for IL-1Ra and negative regulators of IL-1 activity were observed that appear to predict predisposition to inflammation in each location in IL-1Ra knockout mice. The eye is extremely sensitive to locally administered LPS, and IL-1Ra deficiency markedly exacerbates the resulting uveitis.
Conclusion
This study demonstrates that IL-1Ra plays an important role in suppressing local responses in eyes injected with LPS and that there is discordance between murine eyes and joints in the extent to which IL-1Ra protects against spontaneous inflammation.