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Lycianthes bigeminata Bitter (Solanaceae) is an important medicinal herb distributed in the sholas of Nilgiris and chiefly used for curing ulcer. It is reported that the species is present in the sholas with poor population size in comparison to other constituent species. Owing to the demand and subsequent exploitation, it is predicted that it may occupy still poor association in the sholas of Nilgiris in course of time. Hence in vitro regeneration through employing tissue culture technique is needed. The preliminary attempt in the present study reports that the MS medium supplemented with Benzyl Amino Purine (BAP) and Naphthalene Amino Acid (NAA) at 0.5 mg/l each, induced effective callus formation. However further studies on hardening is suggested to know the survivability of this species.