PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of narLink to Publisher's site
 
Nucleic Acids Res. Nov 25, 1990; 18(22): 6531–6535.
PMCID: PMC332606
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.
J G Williams, A R Kubelik, K J Livak, J A Rafalski, and S V Tingey
Central Research and Development Department, E.I. du Pont de Nemours & Co., Inc, Wilmington, DE 19880.
Abstract
Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page.
Images in this article
Articles from Nucleic Acids Research are provided here courtesy of
Oxford University Press