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Logo of bmcpediBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Pediatrics
 
From:
Published online 2012 March 20. doi: 10.1186/1471-2431-12-33

Table 4

Summary of previous studies examining DNA methylation differences in ART conceptions

AuthorTissueART groupSC groupGene/RegionFindings
Oliver, et al. [38]Blood66 (34 IVF, 32 ICSI)59H19, KCNQ1OT1,
SNRPN, IGF2,
LOC388665, INSL5, ARHGAP24, STK19, NCRNA00282, JPH4,
SYP, BEX1
Only significant difference in NCRNA00282 and possibly
ARHGAP24

Zheng, et al. [36]Cord blood101 (40 ICSI, 61 IVF)60KvDMR1, SNRPN,
MEST, MEG3, TNDM,
XIST
All showed normal methylation
patterns

Zheng, et al. [37]Chorionic villus samples44 spontaneous abortions, 22 multifetal reductions45 spontaneous abortions, 47 induced abortionsPEG1/MESTHigher methylation in spontaneous abortions in ART or SC group
compared with other two groups,
no difference between ART and
SC

Wong, et al. [34]Placenta77 (32 IVF, 45 ICSI)12H19/IGF2 ICR1No difference between groups
consisting of IVF, ICSI, SC
stratified by size for gestational
age, 6 ART and 2 SC cases with hypomethylation

Shi, et al. [31]Cord blood6130IGF2/H19 ICRNo difference between groups, but
three ICSI children had abnormal demethylation

Li, et al. [28]Cord blood29 twin pairs30 twin pairsKvDMR1, PEG1,
H19/IGF2 DMR
No significant differences, but
lower methylation in KvDMR1 and
higher methylation in H19/IGF2 in
ART twins.

Zechner, et al. [35]Chorionic villus samples42 spontaneous miscarriages and stillbirths29 abortions and stillbirthsH19, MEG3, LIT1,
MEST, NESP55, PEG3,
SNRPN, NANOG, APC
No significant difference between
groups, more extreme methylation
values in the control group

Turan, et al. [33]Cord blood, cord, placenta4556IGF2/H19, IGF2RThe maternal to paternal
methylation ratio mean and
variance were greater in the IVF
group for both tissues (means in
cord and placenta and variance in
cord blood and cord), no
differences for IGF2R

Tierling, et al. [32]Maternal blood, cord blood, amnion/chorion tissue112 (77 ICSI, 35 IVF)73KvDMR1, H19,
SNRPN, MEST, GRB10, DLK1/MEG3, Ig-DMR, GNAS NESP55, GNAS NESPas, GNAS XL-
alpha-s, GNAS Exon1A
Difference in MEST in cord blood
(IVF vs. ICSI or IVF vs. SC),
higher MEST methylation in IVF
mothers vs ICSI or SC; also
possible differences in
DLK1/MEG3 and the GNAS region
in cord blood

Chen, et al. [23]Embryos320H19 DMR18.7% with hypomethylation

Kobayashi, et al. [27]Trophoblastic villi78 abortions38 non-ART abortionsH19, GTL2, PEG1, KCNQ1OT1, ZAC,
PEG3, SNRPN
Hypomethylation in ART cases
relative to controls for H19 (n = 6),
GTL2 (n = 2), PEG1(n = 1), LIT1
(n = 4), ZAC (n = 1), PEG3 (n = 1)

Katari, et al. [26]Cord blood, placenta1013Genome wide, 736
genes
Differentially methylated CpG
sites tended to have higher
methylation in cord blood and
lower methylation in placenta

Kanber, et al. [25]Buccal cells19 small for gestational age and ICSI29 normal birth weightKCNQ10T1, IGF2/H19, PEG1,
PEG3, PLAGL1, GTL2
1 ICSI child with
hypermethylation of KCNQ1OT1
and PEG1, 2 control children had hypermethylation of GTL2

Gomes, et al. [24]Peripheral blood, Cord blood, placenta12 peripheral blood, 6 cord blood, placenta22 peripheral blood, 8 cord blood, placentaKvDMR13 ART children with
hypomethylation in blood, lower
levels in ART vs. SC, but not
statistically significant

Palermo, et al. [30]Blood550SNRPNNo differences from expected

Manning, et al. [29]Blood92 ICSIReference sampleSNRPNNo differences from expected