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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Sci Transl Med. Author manuscript; available in PMC 2012 July 18.
Published in final edited form as:
PMCID: PMC3320799
NIHMSID: NIHMS359792

Normalization of Notch upregulation reverts large vessels to microvessels via EphB4-mediated venous reprogramming

Summary

High-flow through abnormal blood vessels underlies many life-threatening diseases. The ability to safely and non-invasively normalize these vessels by molecular intervention holds promise to treat these devastating conditions. Here we studied high-flow AV shunts caused by upregulation of Notch signaling via endothelial expression of constitutively-active Notch4 (Notch4*). Using 4D, two-photon imaging with cellular resolution in live mice, we found that normalizing Notch signaling by turning off Notch4* promptly converted large caliber, high-flow AV shunts to capillary-like vessels. The process was initiated by vessel narrowing without the loss of endothelial cells. Restoration of venous receptor EphB4 is an underlying mechanism, as EphB4 expression was recovered upon Notch normalization and required for the vessel regression. The structural regression of the high-flow AV shunts returned shunting flow to perfusing vessels, reversing tissue hypoxia and dysfunction. Our data provide direct, in vivo evidence that a single genetic manipulation in Notch pathway can exert dominant effects over hemodynamics leading to safe degeneration of the high-flow AV shunts at the core of AV Malformations.

Keywords: Arteriovenous malformation, Notch, Angiogenesis, Arterial Specification, Endothelial Cell, Stroke

Introduction

Abnormally enlarged high-flow vessels often continue to grow, leading to life-threatening ruptures. This dangerous vascular lesion underlies the pathology of a wide range of “high-flow” vascular diseases such as Arteriovenous Malformation (AVM), Hereditary Hemorrhagic Telangectasia (HHT) and Aneurysms (1). The hemodynamic stress exerted on the vasculature by these high-flow lesions can cause hemorrhagic rupture (1, 2). The ability to safely and non-invasively constrict the high-flow large vessels by molecular intervention holds promise to treat these life-threatening conditions that currently have limited effective treatments. We demonstrate here that established high-flow AV shunts can regress by a single genetic correction of Notch signaling.

Normally, arteries carry blood from the heart to the capillaries, successively reducing vessel caliber and blood flow. Capillaries, where exchange of nutrients and wastes occurs, are the smallest diameter vessels with lowest blood flow. Post-capillary venules join sequentially wider veins to return blood back to the heart. This arterial-venous (AV) interface is critical for proper tissue perfusion. In AV shunts, the direct connection of arteries to veins displaces the perfusing capillaries, creating high-flow AV shunts that perpetuate lesion growth (2). Thus, high-flow AV shunts are the core defect in AVMs.

Notch receptors and ligands are transmembrane proteins that promote arterial at the expense of venous differentiation by enhancing expression of arterial molecular markers, such as ephrinB2, and suppressing the expression of venous markers, such as EphB4 (3). The transmembrane signaling molecule ephrinB2 was the first gene found to be expressed in the endothelial cells (ECs) of arteries and not veins, and is the most recognized arterial endothelial marker (4). Its cognate tyrosine kinase receptor, EphB4, was the first venous endothelial marker identified (4). COUP-TFII, a member of the orphan nuclear receptor superfamily expressed in venous and not arterial ECs, is upstream of Notch and actively promotes venous differentiation by repressing the expression of Notch (5). These AV specific genes are crucial in the morphogenesis of the embryonic vasculature, and their AV differential expression patterns persist in adult vascular endothelium (6, 7), suggesting post-natal retention of AV specification may have a role in maintaining vascular structure. Supporting this notion, we and others have reported that endothelial Notch activity is aberrantly increased in patients with brain AVMs (8, 9), supporting Notch signaling as a molecular lesion and a therapeutic target of this disease.

Here, using a mouse model of Notch-mediated AVM (10) and two-photon excited fluorescent imaging in live brains (11), we obtained 4D vascular topology and blood velocity data to demonstrate that high-flow AV shunts are remarkably reverted to capillary-like vessels after normalization of Notch, through an EphB4-dependent mechanism, without the loss of ECs.

Results

Repression of Notch4* causes the specific regression of high-flow AV shunts

In our Notch4*-Tet mouse model of AVM, a constitutively-active Notch4 (Notch4*) is specifically expressed in ECs, using a temporally-regulatable tetracycline (Tet)-repressible system (Tie2-tTA; TRE-Notch4*) (12). Repression of Notch4* protein expression by Doxycycline (Dox) treatment was rapid, and approached baseline levels by 24hrs (Fig. S1). Since Notch4* is rapidly repressed by Dox, we use Notch4* repression instead of Dox treatment at times for clarity. Notch4* expression leads to high-flow AV shunts in these mice. To directly test if these high-flow AV shunts normalize after the repression of Notch4*, we combined two-photon microscopy with a cranial window placed over the right parietal cortex (Fig. S2) to visualize live brain vasculature over time.

To avoid the potential confounding effects of hemorrhage and animal illness in late stage, we focused on high-flow AV shunts at early stage, around postnatal day 12 (P12), when most mutants had just developed AV shunts. The minimum diameter of AV shunts at P11-13 averaged 22.2μm ± s.d. 7.3, ranging from 8.1 to 51.3μm (n=46 shunts in 13 mice), approximately 2 to 10 times the diameter of the capillaries in age-matched controls, which averaged 4μm ± s.d. 0.5, ranging from 2.7 to 5.0μm (n=9 capillaries in 3 mice, Fig. S3 & Table S1). Centerline flow velocity through AV shunts was much higher than in control capillaries, averaging 37.7mm/s ± s.d. 14.4 (n=11 shunts in 11 mice), compared to 2.1mm/s ± s.d. 1.0 in control capillaries (n=9 capillaries in 3 mice, Fig. S3 & Table S1), and as reported (13).

We then analyzed the diameter and flow in AV shunts before and after Notch4* repression. We found that both diameter and flow were significantly decreased within 48hrs of Notch4* repression (Fig. 1), relative to untreated mutants (Fig. S4). Diameter changed primarily in the AV shunt and distal vein, adjacent arterial vessels were less affected (Fig. 1). To determine whether advanced AVM also regressed when Notch4* was turned off, we examined severely affected, ataxic mice at a later time point (P22), and found that these mature AVM also did regress (Fig. S5).

Figure 1
Repression of Notch4* induces the normalization of arteriovenous malformation

Not all AV shunts enlarge with continued Notch4* expression, a variability likely caused by systemic hemodynamic instability. Therefore, we sought to identify a subset of “growth-prone” AV shunts to further test the effects of Notch4* repression. We found that among a “growth-prone” population of AV shunts, defined by continued growth over several days, all were induced to regress by Notch4* repression (Fig. S6, 10 AV shunts in 8 mice). In contrast, without treatment, all of this population continued to grow (5 AV shunts in 2 mice).

When imaging for >1 week was possible, we observed the complete regression of the AV shunt to capillary diameter (Fig. S7), a finding also confirmed by ex vivo analysis (Fig. S7). Structurally, this regression involved the normalization of smooth muscle cell coverage (Fig. S8). Narrow time-point analysis indicated that the onset of diameter and velocity reductions coincided and occurred within 12–24hrs of Notch4* repression (Fig. S9). Thus, our data shows that Notch4* repression results in the prompt narrowing of high-flow AV shunts.

Notch4* repression directly induces narrowing of AV shunts

Since reductions in blood flow are known to cause vessel regression (14), we asked whether Notch4* repression leads to shunt regression directly, or indirectly through the reduction of AV shunt flow. To discriminate between these possibilities, we measured blood flow in the upstream feeding artery, the AV shunt, and an adjacent artery.

If Notch4* repression directly reduces the diameter of the AV shunt, we would expect increased resistance and decreased flow through the AV shunt (Fig. 2D). Consequently, the total flow through the feeding artery would also be reduced. Furthermore, the AV shunt blood flow would redistribute to adjacent arteries, thus increasing blood flow in adjacent arteries. Our empirical measurements matched these predictions; total blood flow was reduced, but flow through an adjacent artery to the AV shunt was increased (Fig. 2A–C).

Figure 2
Flow analysis indicates that AVM narrowing is the primary event in AVM regression

Hypothetically, regression of the AV shunt might also be caused indirectly by the effects of reduced flow in the AV shunt following Notch4* repression (Fig. 2D). Two possible scenarios might lead to reduced flow in the AV shunt. In one, resistance in the adjacent artery is reduced, “stealing” blood flow from the AV shunt. However, in this scenario, total systemic resistance to flow should also be reduced, and thus combined flow through the AV shunt and the adjacent artery should be increased, which we do not see. In a second scenario, systemic flow is reduced by events either upstream or downstream of the AV shunt and adjacent artery. However, in this scenario, flow through both the AV shunt and the adjacent artery should be reduced, which we also do not see. Thus, our data supports a direct mechanism for AV shunt regression following Notch4* repression.

Vessel regression was initiated through a mechanism without the loss of endothelial cells

To understand the cellular mechanism of AV shunt regression, we asked whether reduction in the total number of ECs or the area covered by individual ECs was involved. To this end, we used the ephrin-B2-H2B-eGFP mouse line to provide nuclear labeling of ECs within the AV shunt (10). In the presence of Notch4*, ECs in the AV shunt, regardless of arterial or venous origin, expressed ephrin-B2-H2B-eGFP. The H2B-eGFP fusion protein is extremely stable and can persist for months (15). Thus, within the AV shunt, in the short time-frame of examination, ephrin-B2-H2B-eGFP serves as a general EC marker without arterial specificity.

We performed 4D imaging of AV shunt diameters and cell numbers in 23 AV shunts in seven Tie2-tTA;TRE-Notch4*;ephrin-B2-H2B-eGFP mice, at timepoints up to 48hrs following Notch4* repression. We found that all shunts regressed by 40 ± s.d. 14% by the 24 or 28hr timepoint. The onset of AV shunt regression was variable, sometimes occurring as early as 12hrs after Notch4* repression. Approximately 70% (18/23) of these AV shunts regressed without detectable loss of cells at 24 or 28hrs, judged by counting the eGFP+ nuclei. Fig. 3A shows such an example, where AV shunt regression was detected between 20–28hrs, and cell count was not reduced in the regressing AV shunts by 28hrs, nor by 36hrs with further vessel regression. Fig. S10 is another example. These results suggest that the AV shunt regression did not depend on the loss of vascular cells. We confirmed this hypothesis with an alternative method of tracking endothelial nuclei, using our Tie2-tTA induction system in conjunction with a TRE-H2B-eGFP reporter. We first verified that this reporter is a specific and robust marker of endothelial nuclei, as analysis of cell labeling in section revealed that 91.4 ± s.e.m. 3.4% of DAPI+ ECs, and none of the adjacent mural cells, were GFP+ (Fig. S11). We then analyzed vessel diameter and EC number up to 36hrs following Notch4* repression. By 12hrs, we detected regression in 36 of 38 AV shunts from 4 mice examined. Only 9 of the 36 regressing AV shunts exhibited loss of ECs. 27 AV shunts regressed without detectable loss of ECs (Fig. S12B). We did detect ECs loss later (Fig. S12B), after AV shunt regression, but this was not clearly correlated with either shunt diameter or degree of regression (Fig. S12C&D). Fig 3B depicts such an example, where AV shunt regression was detected between 12–20hrs, but cell count was not reduced until 36hrs following Notch4* repression. These data suggested that the initiation of AV shunt regression does not require the loss of ECs.

Figure 3
Regression is initiated by the loss-less reorganization of endothelial cells

Ex vivo staining of VE-cadherin showing cell-cell junctions in mutants before and after repression of Notch4* suggests that the area encompassed by individual ECs was reduced during regression (Fig. S13). Therefore, mean area, but not the total number, of ECs was reduced in the acute regression of AV shunts after Notch4* repression.

Coup-TFII expressing venous endothelium within AV shunts upregulates EphB4 following repression of Notch4*

We then asked whether recovery of venous specification, which was repressed in the presence of Notch4*, might underlie the regression. Coup-TFII is a venous marker upstream of Notch. Therefore, we hypothesized that Coup-TFII expression would be retained in the cells of venous origin in the AV shunt, making it possible to trace the original venous segment and to assess the reestablishment of venous specification upon Notch4* repression. To determine Coup-TFII expression, we used a nuclear lacZ reporter of Coup-TFII promoter activity, Coup-TFII+/fl-stop-nLacZ;Tie2-Cre (5). The lacZ expression is controlled by a floxed-STOP sequence. Tie2-Cre-mediated excision ensures that the lacZ reports the expression of Coup-TFII in Tie2+ endothelial and hematopoetic cell lineages.

In the control, the Coup-TFII expression was localized to the endothelial nuclei of the venous branches, including capillaries, but absent in the arterial branches in the brain (Fig. 4A). In Notch4* mutants (Fig. 4B&C), regardless whether Notch4* was ON or OFF, Coup-TFII expression was maintained throughout the venous branches, as in controls. Thus, Coup-TFII expression clearly marked the venous boundary of AV shunts and suggests that part of the AV shunts originated from the vein.

Figure 4Figure 4
Turning off Notch4* normalizes arteriovenous specification in AV shunts

EphB4, in contrast to Coup-TFII, is a Notch downstream venous marker (4, 16), making it possible to trace the effect of Notch4* on venous repression. We used a lacZ reporter of EphB4 promoter activity (16) to examine EphB4 expression before and after Notch4* repression. In controls, EphB4tau-lacZ was expressed throughout the veins, venules, and into the capillaries of the brains (Fig. 4D). Notch4* expression decreased the expression of EphB4tau-lacZ, resulting in patchy expression in the vein and very little expression in AV shunts (Fig. 4E).

To examine arterial marker expression during regression, we used an ephrin-B2 reporter. We have previously shown that ephrin-B2 expression is upregulated through the AV shunt in Notch4*-On mutants (10). Here, using Ephrin-B2tau-lacZ reporter mice, we confirmed this upregulation (Fig. 4H) and show ephrin-B2 was normalized when Notch4* was turned off (Fig. 4I). To determine whether Notch4* repression leads to the normalization of a broader arterial specification program in the AV shunts, we examined the expression of additional arterial specific proteins Dll4, Jag1, and Cx40 (Fig. 4J–U). We found that all of these genes were expressed preferentially in the arteries in controls, extended through AV shunts and veins when Notch4* was on in mutants, and normalized when Notch4* was turned off.

These data combined with the Coup-TFII expression pattern suggest that Notch4* induced arterial identity and repressed venous identity in the venous segment. Repression of Notch4* resulted in the loss of arterial markers ephrin-B2, Dll4, Jag1 and Cx40 (Fig. 4I,O,R,U) with a concomitant increase in EphB4 expression in the regressing AV shunts (Fig. 4F).

To determine whether EphB4 was re-expressed at the cellular level, we used the TRE-H2B-eGFP reporter to mark ECs in Tie2-tTA; TRE-Notch4*; TRE-H2B-eGFP mice expressing Notch4* transgenes by eGFP expression, (Fig. 5A). We found that EphB4 protein expression was repressed to ~50% of control levels in the TRE-H2B-eGFP+venous ECs of Tie2-tTA; TRE-Notch4*; TRE-H2B-eGFP mutants, relative to Tie2-tTA; TRE-H2B-eGFP controls. Once the Notch4* transgene was turned off, EphB4 expression normalized to control levels in the venous cell population.

Figure 5
Venous marker EphB4 is re-expressed in venous endothelial cells and required for AV shunt regression

Inhibition of EphB4 signaling impairs regression of AV shunts

To determine whether EphB4 signaling is necessary for the regression of AV shunts, we used a soluble form of the EphB4 receptor to competitively inhibit EphB4 receptor signaling (17) following repression of Notch4* (Fig. 5B). If regression depends upon EphB4 signaling, the soluble EphB4 receptor (sEphB4) should inhibit the reduction in AV shunt diameter induced by suppression of Notch4*. Indeed, the mean change in the diameter of AV shunts (−17.8% ± s.d. 24.1) was significantly reduced relative to mice not treated with sEphB4 (−47% ± s.d. 19.3). As a control for the recombinant protein treatment, we examined regression in mice injected with recombinant human fibronectin. The mean change in AV shunt diameter in these mice (35.1% ± s.d. 26.2) was not significantly different from the regression in mice without recombinant protein treatment (P<0.05). Thus, sEphB4 significantly impaired the regression of AV shunts.

Regression of AV shunts alleviates hypoxia

To investigate the functional effect of AV shunt regression, we asked whether AV shunt regression reversed vascular dysfunction. We first examined blood velocity in arterial branches adjacent to the AV shunt and found blood velocity increased with Notch4* repression but decreased with continued Notch4* expression (Fig. 2C). Strikingly, these decreasing velocities could be promptly increased by Notch4* repression and shunt regression (Fig. S6). Lectin perfusion of capillary vessels (Fig. 6A–C) suggests that tissue perfusion was globally increased following AV shunt regression.

Figure 6
Repression of Notch4* normalizes vascular perfusion, oxygenation, tissue structure

We then examined hypoxia in Notch4* mutants with neurologic defects before and after Notch4* repression, using hypoxyprobe staining. Hypoxyprobe detects tissue exposed to a partial oxygen pressure of <10mmHg, close to the hypoxic threshold expected to cause dysfunction of neuronal cells (18). We detected an increase in hypoxyprobe staining in the cerebellum and cerebral cortex of sick Notch4*-expressing mice, relative to their littermate controls (Fig. 6D&F). When Notch4* was repressed for 72hrs in severely affected Notch4* mutants, hypoxyprobe staining intensity was significantly reduced, and approached control levels (Fig. 6E).

Finally, we determined the histopathological changes in the brain parenchyma with and without Notch4* repression. Histological analysis of Notch4* mutants without Notch4* repression revealed foci of pyknotic nuclei, often surrounding a core of decreased nuclear density, consistent with ischemia-induced necrosis (4 of 6 mice, Fig. 6G). Such regions occasionally also contained evidence of hemorrhage. Notch4* repression for several weeks eliminated these pyknotic and acellular regions (9 of 9 mice), although structural damage could still be detected (Fig. 6H), presumably representing the evolution of the earlier ischemic damage. In support of this, hemosiderin depositions suggested the resolution of earlier hemorrhages. These findings suggest structural healing of earlier lesions after Notch4* repression. Thus, regression of AV shunts induced by Notch4* repression normalizes cerebrovascular flow patterns and tissue oxygenation, providing a physiologic explanation for recovered brain function.

Discussion

Here, we report that genetic reprogramming of AV specification converts high-flow AV shunts to low-flow microvessels. Using in vivo time-lapse imaging with single-cell resolution, we show that Notch4* repression leads to a narrowing of AV shunts which does not require the loss of ECs, initiating AV shunt regression. Mechanistically, this involves the restoration of venous programming in the high-flow AV shunts by endothelial Notch4* repression.

Notch induces reversible arterial programming of the venous compartment

Previously, we have observed expanded expression of the arterial marker ephrin-B2 in the vasculature following upregulation of Notch signaling in mice (10, 12, 19). It is also reported that the arterial ECs in coronary artery development arise from venous vessels (20). However it is currently unknown whether venous ECs had been arterialized, or if arterial ECs had expanded. Using venous markers upstream (Coup-TFII) (5) and downstream (EphB4) (19) of Notch, we now show that Notch is sufficient to cause the arterialization of differentiated venous endothelium in the postnatal mouse. We show here, in normal brain endothelium, that Coup-TFII is preferentially expressed in venous and not arterial endothelium. Upstream of Notch, Coup-TFII expression is not affected by Notch4* expression, identifying ECs of venous origin. Expression of Notch4* led to the mis-expression of arterial markers ephrin-B2, Dll4, Jag1 and Cx40 in Coup-TFII positive veins, confirming that Notch4* expression converts venous ECs into arterial ECs. Expression of Notch4* also led to the suppression of the venous marker EphB4 in the Coup-TFII positive cells, demonstrating a simultaneous loss of venous expression in ECs of venous lineage. Besides AV marker expression, Notch4*-converted venous segments also exhibit the features of arteries, including arterial structure and flow velocity. Thus, our data demonstrate that Notch is sufficient to genetically arterialize veins.

We further demonstrate that this arterialization of veins by Notch upregulation is reversible. Notch4* repression led to re-expression of venous marker EphB4 in Coup-TFII positive vessels, as well as structural and hemodynamic normalization. Thus, our results suggest that venous vessels arterialized by Notch4* were reverted upon repression of Notch4*. The reversible arterial specification in post-natal vasculature suggests that AV lineage specification is genetically pliable, and a single genetic manipulation is sufficient to switch AV specification postnatally.

AV reprogramming elicits the narrowing of high-flow AV shunts without the loss of ECs

The mechanism underlying the regression of AV shunts following Notch4* repression involves ephrin-B2 and EphB4-mediated EC reorganization, rather than a reduction of EC number. While the role of Eph/ephrin signaling in the endothelium is not yet clear, our finding is consistent with the established functions of ephrin-B2/EphB4 in regulating cell migration through repulsive cues (21). We think that once re-expressed in Coup-TFII+ ECs, EphB4 mediates ephrin-B2 signaling and elicits EC repulsion (Fig. 7). Supporting a critical role for ephrin-B2/EphB4 signaling in this normalization process, the specific regression of vessels occurs at the AV interface, while the adjacent arteries often do not regress. Furthermore, the regression of AV shunts following Notch4* repression can be blocked by sEphB4.

Figure 7
Model for AVM regression: normalization of arteriovenous programming elicited by repression of Notch4* initiates AVM narrowing

The loss-less mechanism of AV normalization following Notch4* repression is distinct from the apoptotic mechanism of vessel regression following VEGF withdrawal. Microvessels in tumors and normal tissues regress following VEGF inhibition (22). Regression in these vessels is attributed to apoptosis of ECs (23). Another model of vessel regression is the hyaloid vasculature of the eye (24). In vivo imaging of hyaloid vessel regression shows that the apoptosis of ECs obstructs the lumen and capillary blood flow, triggering the apoptosis of remaining ECs in the capillary segment and ultimately its regression (25). Thus, apoptosis and a subsequent reduction in blood flow are thought to precipitate vessel regression in these settings.

Our findings suggest that the cellular mechanism underlying the regression of the high-flow vessels following Notch4* repression does not involve EC apoptosis, but likely EC reorganization.

Future implications

Our direct in vivo imaging demonstrates the regression of high-flow large vessels to capillary-like vessels by a single genetic manipulation. Strikingly, the vascular normalization was not accompanied by hemorrhage and vascular damage. Rather, AV shunt regression safely reversed tissue hypoxia and tissue dysfunction. We have focused on brain AVMs, but the finding likely applies to AVMs in other tissues, since we have previously identified Notch4* mediated AVMs in the liver, skin, uterus and lung in the mouse (12, 19, 26). Thus, we believe that exploiting the tractable BAVM model system will provide important clues into the cellular and molecular regulation of AVMs in general. AV shunts are a core component of a range of “high-flow” vascular lesions (1). Thus, our demonstration of complete and safe normalization of dangerous high-flow AV shunts in animals may inspire molecular therapeutic strategies to induce the regression of these dangerous high-flow vessels and treat these devastating diseases.

Materials and Methods

Mice

Tie2-cre, Tie2-tTA and TRE-Notch4* mice are published (8, 10, 12, 19), as are Ephrin-B2+/H2B-eGFP (27), EphB4+/tau-lacZ (16), ephrin-B2+/tau-lacZ (4), mT/mG (28), TRE-H2B-eGFP (29), and Coup-TFII+/fl-stop-nLacZ mice (30). Tetracycline solution (0.5 mg/ml Tet, 50 mg/ml sucrose, Sigma) was administered to mothers and withdrawn at birth (10). Doxycycline treatment was initiated with intraperitoneal injection (500μL of 1 mg/mL in PBS), followed by doxycyline diet (200-mg/kg diet, Bio-Serv) (10, 12). All animals were treated in accordance with the guidelines of the University of California San Francisco Institutional Animal Care and Use Committee.

Soluble EphB4 treatment

200μL of 200μg/mL recombinant human EphB4 extracellular domain (R&D systems) was injected by tail vein (final concentration of ~4mg/kg), followed by 100μL of 200μg/mL 24hrs later (17). Recombinant human fibronectin (R&D systems) at the same concentration was a negative control.

In vivo imaging

Chronic in vivo brain vascular imaging was performed as described (11, 31), with modifications for immature mice (32). Briefly, a craniotomy was performed over the right cortex. A 5mm glass coverslip (World Precision Instruments) was placed over artificial cerebrospinal fluid and fixed into place. A custom metal bar was attached adjacent to the window, allowing it to be secured by a custom adaptor arm on a stereotaxic base (Cunningham). For imaging, mice were anesthetized with isoflurane (1.25–1.5%) in pure oxygen on homeothermic heat blanket (Harvard Apparatus). Fluorescent contrast agents were injected by tail vein [2000 kDa FITC-dextran (Sigma), 155 kDa TRITC-dextran (Sigma), or 2000 kDa Texas Red-dextran (prepared according to published protocols (33) and filtered by dialysis)]. Two-photon microscopy was performed with a locally constructed microscope, to be described in detail in a future publication.

Immunostaining

Conjugated lycopersicon esculentum-lectin (Vector Labs) was injected as we described (10, 12). Brain was fixed by 1% paraformaldehye (PFA) fixation via the left ventricle. Tissue was incubated in blocking solution (2% bovine serum albumin, 0.1% TritonX-100 in PBS), primary antibody overnight, and secondary antibody overnight. Antibodies were anti-VE-Cadherin (BD Pharminogen 555289, Clone 11D4.1, 1:200 dilution) and anti-α-SMactin (Sigma F377, Clone 1A4, 1:200 dilution) (12, 34). Staining for AV marker expression followed a similar protocol, except that blocking was with 10% donkey serum, 0.1% TritonX-100 in PBS. Antibodies (2ug/mL in block) were anti-Notch4-ICD (Millipore, 07–189), anti-Jag1 (R&D Systems BAF599), anti-Dll4 (R&D Systems, BAF1389), and anti-Cx40 (Santa Cruz, sc-20466).

Notch4 staining followed published protocols (10). Briefly, brains were perfusion-fixed with or without prior lycopersicon esculentum-lectin (Vector Labs) injection. After overnight fixation in 1% PFA, brains were sagittally bisected and dehydrated in 30% sucrose in PBS overnight and imbedded in OCT. 10μm sections were cut, blocked (3% donkey serum, 2% BSA, 0.2% TritonX-100 in PBS), and then incubated with anti-Notch4 ICD antibody (Millipore 07–189, formerly Upstate, 1:500 dilution) overnight in block, washed, incubated with secondary, washed and stored in VectaShield +DAPI (Vector Labs).

X-gal/DAB co-staining

Under ketamine/xylazine and isoflurane anesthesia, 25μg biotinylated lycopersicon esculentum-lectin (Vector Labs) was injected via inferior vena cava and allowed to circulate for 2 minutes. Perfusion was performed through the left ventricle with PBS, followed by fixative (0.25% gluteraldehyde, 50mM EGTA and 100mM MgCl2 in PBS). After short fixation, cortex was stained for beta-galactosidase at room temperature according to published X-gal protocols (35). Cortex was then fixed with 1% PFA, and blocked (10% bovine serum albumin and 0.1% TritonX-100 in PBS), incubated with 1:1000 streptavidin conjugated horse-radish peroxidase (Jackson Immuno) in block, washed and stained with 3,3′-diaminobenzidine kit (Vector Labs).

Supplementary Material

Supplementary data

Fig. S1. Notch4* repression occurs within 24hrs of doxycycline treatment

Frozen sagittal brain sections with fluorescent immunostaining for Notch4 intracellular domain, with labeling of perfused vessels by tomato lectin staining, and nuclear labeling by DAPI. Images show overlap of Notch4 stain with DAPI in cells lining lectin-perfused vessels. Isolation and amplification of the Notch4 staining shows light Notch4 signal in the control vessel nuclei, strong expression in the vessels of mutants before Notch4* suppression (0hrs Notch4*-Off), and light expression after 24hrs doxycycline treatment (24hrs Notch4*-Off). Graph shows quantification of fluorescent intensity in endothelial cell nuclei of brain vasculature in Notch4* mutants before and after Dox treatment, and their littermate controls. Error bars represent s.d. between individual animals (n=6 at 0hrs Notch4*-Off, n=6 at 24hrs Notch4*-Off, and n=6 controls). In each animal, three nuclei in each of three vessels were quantified to yield an average intensity. *P=0.0007.

Fig. S2. Placement of the chronic imaging window

Perfusion of the whole brain with fluorescein-labeled agarose shows pial arteries (arrowheads) and the typical placement of the window (boxed area).

Fig. S3. Normal blood velocities in artery, capillary and vein in wild-type mice

Two-photon timelapse imaging of cortical brain vessels through cranial window in wild-type mouse. Plasma labeling was provided by intravenous FITC-dextran. (A) Line depicts the path of blood from artery through arteriole, capillary, venule and vein. (B) Images of the artery, capillary and vein in which blood velocity was measured by line scan along the axis depicted. Diameters of the vessels were measured transaxially. (C) Velocity tracing, as calculated from line scans. Note that both velocity and the pulse (the range in velocity) were reduced from artery to capillary to vein.

Fig. S4. Specific regression of AV shunts after repression of Notch4*

Two-photon timelapse imaging of cortical brain vessels through cranial window in Notch4* mutant mice. Plasma labeling was provided by intravenous FITC-dextran. (A) Regression of existing AV shunts following 48hrs of Notch4* repression (B) Regression did not occur without Notch4* repression. Note the reduction in the size of the distal vessels to the enlarging AV shunt in B. Scale bars = 100μm.

Fig. S5. Repression of Notch4* induces regression of well-established large AV malformation

Two-photon timelapse imaging of cortical brain vessels in Notch4* mutant mice after well-established AV malformation. Vessel topology was visualized by intravenous FITC-dextran. Windows were placed on mice demonstrating ataxic symptoms, corresponding with advanced-stage disease. The diameters of large AV shunts were reduced following repression of Notch4* (n = 20 AV shunts in 3 mice). The majority of regression occurred rapidly within the first week and continued to two weeks. Detection of FITC-dextran-perfused capillaries adjacent to AV malformations increases over time. Scale bars = 100μm.

Fig. S6. AV shunts are stable until repression of Notch4*

(A–C) Color-coded depiction of the AV shunt imaged by two-photon timelapse imaging in (D–F), arteries are in red and veins are in blue. AV shunt remained for 24 hours without repression of Notch4* (Compare D and E), but regressed with 48hrs of Notch4* repression (Compare E and F). Bottom panels show velocity traces obtained by line scan in the vessels indicated by yellow or green arrows (A–C). Velocity in AV shunt feeding artery (green) decreased, while velocity in the non-AV shunt artery increased (yellow), during regression of the AV shunt. Figure is representative of results in 10 AV shunts in 8 mice. Scale bars = 100μm.

Fig. S7. Regression of AV shunts to capillary diameter vessels in mice with and without cranial window

(A) Two-photon timelapse imaging of cortical brain vessels through cranial window in Notch4* mutant mouse. Plasma labeling was provided by intravenous FITC-dextran. Large AV shunt regressed to capillary diameter within 9 days of Notch4* repression. (B) Ex vivo imaging of whole mount cortex from mice perfused with FITC-lectin. Prior to Notch4* repression, large AV shunts were observed between artery and vein. In a similarly affected littermate mutant, following 28 days of Notch4* repression, AV connections were reduced to the diameter of capillaries in littermate control mice. n=3 mutants before Notch4*-Off, n=5 littermate mutants after Notch4*-Off, n=5 controls. Scale bars = 100μm

Fig. S8. Smooth muscle coverage is normalized by suppression of Notch4*.

Whole mount immuno-staining of cerebral cortex. VE-cadherin staining shows vasculature and outline of individual ECs. α-Smooth muscle actin staining shows smooth muscle cell coverage and alignment. Note the reduction in smooth muscle cell coverage in the vein after repression of Notch4*, relative to the littermate mutant before Notch4* repression. n=3 Notch4*-On mutants, n=5 Notch4*-off mutants, n=5 controls. Scale bars = 200μm.

Fig. S9. Velocity changes coincide with narrowing of AV shunts and distal vein beginning by 12–24hrs after Notch4* repression

In vivo timelapse imaging of Tie2-tTA; TRE-Notch4*; Tie2-cre; mT/mG mice (green endothelial fluorescence), with TRITC-dextran labeling of plasma (red). (A) With repression of Notch4*, AV shunt regression began between 12 and 24hrs, coinciding with a reduction in blood velocity through the AV shunt. (B) Without repression of Notch4*, AV shunt diameter and velocity were maintained. Note the reduction of distal vessel size. Blood flow was measured in this distal vessel, and was reduced over time.

Fig. S10. Narrowing of ephrinB2-GFP+ AV shunt occurs specifically after Notch4* repression

(A–C) Two-photon time-lapse imaging through a cranial window following repression of Notch4* in Tie2-tTA; TRE-Notch4*; ephrinB2+/H2B-eGFPmutant with labeling of plasma by Texas-Red dextran. An AV shunt showed little change in diameter or centerline velocity (bottom left corner) after 24hrs without repression of Notch4* (B). Within 24hrs of Notch4* repression (C), the AV shunt was greatly reduced in diameter, with a reduction in blood velocity. Note that the same number of cells were present, but grouped closer. Scale bars = 100μm.

Fig. S11. Tie2-tTA; TRE-H2B-eGFP marks brain endothelial cells.

Imaging of GFP+ endothelial cells from sectioned brain specimens of Tie2-tTA; TRE-GFP; TRE-Notch4* mice perfused with Cy5 bound tomato-lectin. Endothelial nuclei were identified by co-localization of DAPI staining and Cy5 signal in cells along the wall of patent vessel lumens. Nuclei fully enveloped by Cy5 signal were considered endothelial cells (closed arrow heads) and other vascular cells were not (open arrow heads). Triple-color merged image showing DAPI, GFP+ nuclei, and Cy5-labeled endothelium. Two-color merged imaged showing GFP+ nuclei and Cy5-labeled endothelium. Scale bars = 20μm. Quantification of GFP+ endothelial cells from 446 analyzed nuclei in 3 mice.

Fig. S12. Loss of endothelial cells is not required for AV shunt regression

(A) The table shows the number of AV shunts, over time (columns), binned by the percentage of starting diameter (rows). The bottom panel shows average diameter of all 38 AV shunts and corresponding s.e.m. by percentage of starting diameter. (B) Loss of TRE-H2B-eGFP marked cells in AV shunt regression. The table shows the number of AV shunts, over time (columns), binned by the percentage of retained cells (rows). While individual AV shunts displayed variability, initiation of regression by 12hrs usually exhibited no cell loss. Cell number trended downward over time, though many AV shunts retained most or all of their GFP+ endothelial cells. (C) There was no apparent correlation between the percentage of cell loss and the degree of shunt regression by 28hrs. (D) There was no apparent correlation between the percentage of cell loss by 28 hours and the starting AV shunt diameter.

Fig. S13. Endothelial cells are narrowed in regressing AV shunts

(A–D) Fluorescent immunostaining of VE-cadherin in Notch4* mutant mice, imaged in whole mount. Areas shown in A&B were imaged by confocal microscopy at high magnification. Note that the dimensions of endothelial cells were reduced both axially and paraxially along the AV shunt. EC area was reduced (n=4 shunts in one 0hrs Notch4*-Off mouse: 546μm2 ± s.d. 193μm) vs. (n=4 shunts in each of two 48hrs Notch4*-Off mice: 239μm2 ± s.d. 68μm2 and 211.4μm2 ±s.d. 60μm2).

Acknowledgments

We thank Claudia Tomas-Miranda and Dr. Weiya Jiang for experimental assistance, Dr. Sophia Tsai for the Coup-TFII+/fl-stop-nLacZ mice, Dr. Richard Daneman for critical reading, and members of our laboratory for helpful discussions. This work was supported by the Foundation for Accelerated Vascular Research (formerly the Pacific Vascular Research Foundation) and the Frank A. Campini Foundation, the Mildred V Strouss Trust, NIH R01 HL075033, NIH RO1 NS067420, and AHA Grant in Aid 10GRNT4170146 to R.A.W, and AHA 0715062Y and TRDRP 18DT-0009 Predoctoral Fellowships to P.A.M. and TRDRP 19DT- 007 and NIH F30 1F30HL099005-01A1 Predoctoral Fellowships to T.N.K.

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