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Biomark Insights. 2012; 7: 27–37.
Published online Mar 26, 2012. doi:  10.4137/BMI.S8452
PMCID: PMC3320115
Increased Levels of Circulating and Tissue mRNAs of Oct-4, Sox-2, Bmi-1 and Nanog is ESCC Patients: Potential Tool for Minimally Invasive Cancer Diagnosis
Kriti Bahl,1 Anoop Saraya,2 and Rinu Sharma3
1M.Tech, University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector 16 C, Dwarka, New Delhi-110075
2Professor, Department of Gastroenterology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029
3Assistant Professor, University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector 16 C, Dwarka, New Delhi-110075
Corresponding author email: rinusharma/at/rediffmail.com
Abstract
Background
Early stages of esophageal cancer lack a specific symptom, a reliable biomarker and accurate non-invasive diagnostic modalities prompting the pressing need for identification of a marker for early diagnosis of this disease.
Methods
In the present study we investigated the levels of circulating and tissue mRNAs of Oct-3/4, Sox-2, Nanog and Bmi-1 in esophageal cancer patients using Reverse-Transcription Polymerase Chain Reaction (RT-PCR) with the aim of evaluating their potential as minimally invasive diagnostic markers.
Result
Increased transcript levels of Oct-4, Sox-2, Bmi-1 and Nanog were detected in (92%), (95%), (75%) and (67%) of the esophageal cancer tissues, respectively as compared with the matched distant normals.
Conclusion
Interestingly, most of the preneoplastic tissues exhibited increased transcript levels of these stemness markers suggesting their role in early stages of esophageal tumorigenesis. Furthermore, the detection of elevated levels of circulating mRNAs of Oct-4 and Nanog in sera of esophageal cancer patients emphasizes their potential as minimally invasive diagnostic markers for esophageal cancer.
Keywords: Oct3/4, Sox-2, Bmi-1, Nanog, pluripotent
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