PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of narLink to Publisher's site
 
Nucleic Acids Res. 1990 August 11; 18(15): 4409–4416.
PMCID: PMC331258

The 26S rRNA binding ribosomal protein equivalent to bacterial protein L11 is encoded by unspliced duplicated genes in Saccharomyces cerevisiae.

Abstract

Transformant phages expressing L15, a yeast ribosomal protein which binds to 26S rRNA and interacts with the acidic ribosomal proteins, were isolated by screening a yeast cDNA expression library in lambda gt11 with specific monoclonal antibodies. Using yeast DNA HindIII fragments that hybridize with the cDNA insert from the L15-expressing clones, minilibraries were prepared in pUC18, which were afterward screened with the same cDNA probe. In this way, plasmids carrying two different types of genomic DNA inserts were obtained. The inserts were subcloned and sequenced and we found a similar coding sequence in both cases flanked by 5' and 3' regions with very low homology. Sequences homologous to the consensus TUF-binding UAS boxes are present in the 5' flanking regions of both genes. Southern analysis revealed the presence of two copies of the L15 gene in the Saccharomyces cerevisiae genome, which are located in different chromosomes. The encoded amino acid sequence corresponds, as expected, to protein L15 and shows a high similarity to bacterial ribosomal protein L11.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Strycharz WA, Nomura M, Lake JA. Ribosomal proteins L7/L12 localized at a single region of the large subunit by immune electron microscopy. J Mol Biol. 1978 Dec 5;126(2):123–140. [PubMed]
  • Dijk J, Garrett RA, Müller R. Studies on the binding of the ribosomal protein complex L7/12-L10 and protein L11 to the 5'-one third of 23S RNA: a functional centre of the 50S subunit. Nucleic Acids Res. 1979 Jun 25;6(8):2717–2729. [PMC free article] [PubMed]
  • Beauclerk AA, Cundliffe E, Dijk J. The binding site for ribosomal protein complex L8 within 23 s ribosomal RNA of Escherichia coli. J Biol Chem. 1984 May 25;259(10):6559–6563. [PubMed]
  • Ballesta JP, Vazquez D. Activities of ribosomal cores deprived of proteins L7, L10, L11 and L12. FEBS Lett. 1974 Nov 15;48(2):266–270. [PubMed]
  • van Agthoven A, Kriek J, Amons R, Möller W. Isolation and characterization of the acidic phosphoproteins of 60-S ribosomes from Artemia salina and rat liver. Eur J Biochem. 1978 Nov 15;91(2):553–565. [PubMed]
  • Reyes R, Vázquez D, Ballesta JP. Peptidyl transferase center of rat-liver ribosome cores. Eur J Biochem. 1977 Feb 15;73(1):25–31. [PubMed]
  • Vidales FJ, Sanchez-Madrid F, Ballesta JP. The acidic proteins of eukaryotic ribosomes. A comparative study. Biochim Biophys Acta. 1981 Nov 27;656(1):28–35. [PubMed]
  • Sánchez-Madrid F, Reyes R, Conde P, Ballesta JP. Acidic ribosomal proteins from eukaryotic cells. Effect on ribosomal functions. Eur J Biochem. 1979 Aug 1;98(2):409–416. [PubMed]
  • Juan-Vidales F, Sánchez Madrid F, Saenz-Robles MT, Ballesta JP. Purification and characterization of two ribosomal proteins of Saccharomyces cerevisiae. Homologies with proteins from eukaryotic species and with bacterial protein EC L11. Eur J Biochem. 1983 Nov 2;136(2):275–281. [PubMed]
  • Vidales FJ, Robles MT, Ballesta JP. Acidic proteins of the large ribosomal subunit in Saccharomyces cerevisiae. Effect of phosphorylation. Biochemistry. 1984 Jan 17;23(2):390–396. [PubMed]
  • Mitsui K, Tsurugi K. Identification of A1 protein as the fourth member of 13 kDa-type acidic ribosomal protein family in yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1989 Jun 30;161(3):1001–1006. [PubMed]
  • Sáenz-Robles MT, Vilella MD, Pucciarelli G, Polo F, Remacha M, Ortíz BL, Vidales FJ, Ballesta JP. Ribosomal protein interactions in yeast. Protein L15 forms a complex with the acidic proteins. Eur J Biochem. 1988 Nov 15;177(3):531–537. [PubMed]
  • el-Baradi TT, de Regt VC, Einerhand SW, Teixido J, Planta RJ, Ballesta JP, Raué HA. Ribosomal proteins EL11 from Escherichia coli and L15 from Saccharomyces cerevisiae bind to the same site in both yeast 26 S and mouse 28 S rRNA. J Mol Biol. 1987 Jun 20;195(4):909–917. [PubMed]
  • Towbin H, Ramjoué HP, Kuster H, Liverani D, Gordon J. Monoclonal antibodies against eucaryotic ribosomes. Use to characterize a ribosomal protein not previously identified and antigenically related to the acidic phosphoproteins P1/P2. J Biol Chem. 1982 Nov 10;257(21):12709–12715. [PubMed]
  • Elkon KB, Parnassa AP, Foster CL. Lupus autoantibodies target ribosomal P proteins. J Exp Med. 1985 Aug 1;162(2):459–471. [PMC free article] [PubMed]
  • Mitsui K, Motizuki M, Endo Y, Yokota S, Tsurugi K. Characterization of a novel acidic protein of 38 kDa, A0, in yeast ribosomes which immunologically cross-reacts with the 13 kDa acidic ribosomal proteins, A1/A2. J Biochem. 1987 Dec;102(6):1565–1570. [PubMed]
  • Mitsui K, Nakagawa T, Tsurugi K. On the size and the role of a free cytosolic pool of acidic ribosomal proteins in yeast Saccharomyces cerevisiae. J Biochem. 1988 Dec;104(6):908–911. [PubMed]
  • Shimmin LC, Ramirez C, Matheson AT, Dennis PP. Sequence alignment and evolutionary comparison of the L10 equivalent and L12 equivalent ribosomal proteins from archaebacteria, eubacteria, and eucaryotes. J Mol Evol. 1989 Nov;29(5):448–462. [PubMed]
  • Nielsen PJ, McMaster GK, Trachsel H. Cloning of eukaryotic protein synthesis initiation factor genes: isolation and characterization of cDNA clones encoding factor eIF-4A. Nucleic Acids Res. 1985 Oct 11;13(19):6867–6880. [PMC free article] [PubMed]
  • Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. [PubMed]
  • Langridge J, Langridge P, Bergquist PL. Extraction of nucleic acids from agarose gels. Anal Biochem. 1980 Apr;103(2):264–271. [PubMed]
  • Kearney JF, Radbruch A, Liesegang B, Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed]
  • Young RA, Davis RW. Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194–1198. [PubMed]
  • Hattori M, Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986 Feb 1;152(2):232–238. [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Gribskov M, Burgess RR. Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins. Nucleic Acids Res. 1986 Aug 26;14(16):6745–6763. [PMC free article] [PubMed]
  • Mortimer RK, Schild D. Genetic map of Saccharomyces cerevisiae, edition 9. Microbiol Rev. 1985 Sep;49(3):181–213. [PMC free article] [PubMed]
  • Sharp PM, Tuohy TM, Mosurski KR. Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes. Nucleic Acids Res. 1986 Jul 11;14(13):5125–5143. [PMC free article] [PubMed]
  • Warner JR. Synthesis of ribosomes in Saccharomyces cerevisiae. Microbiol Rev. 1989 Jun;53(2):256–271. [PMC free article] [PubMed]
  • Mitra G, Warner JR. A yeast ribosomal protein gene whose intron is in the 5' leader. J Biol Chem. 1984 Jul 25;259(14):9218–9224. [PubMed]
  • Nieuwint RT, Molenaar CM, van Bommel JH, van Raamsdonk-Duin MM, Mager WH, Planta RJ. The gene for yeast ribosomal protein S31 contains an intron in the leader sequence. Curr Genet. 1985;10(1):1–5. [PubMed]
  • Pikielny CW, Teem JL, Rosbash M. Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: implications for U1 RNA and metazoan mRNA splicing. Cell. 1983 Sep;34(2):395–403. [PubMed]
  • Langford CJ, Klinz FJ, Donath C, Gallwitz D. Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast. Cell. 1984 Mar;36(3):645–653. [PubMed]
  • Mager WH. Control of ribosomal protein gene expression. Biochim Biophys Acta. 1988 Jan 25;949(1):1–15. [PubMed]
  • Struhl K. Molecular mechanisms of transcriptional regulation in yeast. Annu Rev Biochem. 1989;58:1051–1077. [PubMed]
  • Woudt LP, Mager WH, Nieuwint RT, Wassenaar GM, van der Kuyl AC, Murre JJ, Hoekman MF, Brockhoff PG, Planta RJ. Analysis of upstream activation sites of yeast ribosomal protein genes. Nucleic Acids Res. 1987 Aug 11;15(15):6037–6048. [PMC free article] [PubMed]
  • Nieuwint RT, Mager WH, Maurer KC, Planta RJ. Mutational analysis of the upstream activation site of yeast ribosomal protein genes. Curr Genet. 1989 Apr;15(4):247–251. [PubMed]
  • Hamil KG, Nam HG, Fried HM. Constitutive transcription of yeast ribosomal protein gene TCM1 is promoted by uncommon cis- and trans-acting elements. Mol Cell Biol. 1988 Oct;8(10):4328–4341. [PMC free article] [PubMed]
  • Vignais ML, Woudt LP, Wassenaar GM, Mager WH, Sentenac A, Planta RJ. Specific binding of TUF factor to upstream activation sites of yeast ribosomal protein genes. EMBO J. 1987 May;6(5):1451–1457. [PubMed]
  • Dorsman JC, Doorenbosch MM, Maurer CT, de Winde JH, Mager WH, Planta RJ, Grivell LA. An ARS/silencer binding factor also activates two ribosomal protein genes in yeast. Nucleic Acids Res. 1989 Jul 11;17(13):4917–4923. [PMC free article] [PubMed]
  • Remacha M, Santos C, Ballesta JP. Disruption of single-copy genes encoding acidic ribosomal proteins in Saccharomyces cerevisiae. Mol Cell Biol. 1990 May;10(5):2182–2190. [PMC free article] [PubMed]
  • Remacha M, Sáenz-Robles MT, Vilella MD, Ballesta JP. Independent genes coding for three acidic proteins of the large ribosomal subunit from Saccharomyces cerevisiae. J Biol Chem. 1988 Jul 5;263(19):9094–9101. [PubMed]
  • Newton CH, Shimmin LC, Yee J, Dennis PP. A family of genes encode the multiple forms of the Saccharomyces cerevisiae ribosomal proteins equivalent to the Escherichia coli L12 protein and a single form of the L10-equivalent ribosomal protein. J Bacteriol. 1990 Feb;172(2):579–588. [PMC free article] [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press