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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2012 September 7.
Published in final edited form as:
PMCID: PMC3306617
NIHMSID: NIHMS362335

Contextual Fear Memories Formed in the Absence of the Dorsal Hippocampus Decay Across Time

Abstract

Mammals suffering damage to the hippocampus display a dramatic loss of explicit, recently formed memories (retrograde amnesia). In contrast, deficits in the ability to form new memories following hippocampal damage (anterograde amnesia) can be overcome with sufficient training. By combining contextual fear conditioning with lesions of the dorsal hippocampus in rats, we discovered that while animals can form long-term contextual fear memories in the absence of the hippocampus, these memories decay with time, lacking the permanence that is a hallmark characteristic of normal fear memories. These findings indicate that while it is initially possible to acquire explicit memories when the hippocampus is compromised, these memories cannot transfer from a recent to remote state. This suggests that memories formed outside the hippocampus may nevertheless require the hippocampus to undergo systems consolidation, which has important clinical implications for the treatment of memory disorders.

Keywords: hippocampus, context fear, memory, forgetting, anterograde amnesia

Introduction

The hippocampus is a critical structure for many forms of memory. Loss of the hippocampus due to pathology or surgery in humans or from targeted experimental ablations in animals produces a profound amnesia (Squire, 1992). Memory for events prior to damage is lost in a manner that is temporally graded (retrograde amnesia), such that memory loss is greatest for events proximal to the insult and lessens for older memories (Zola-Morgan & Squire, 1990; Kim & Fanselow, 1992; Anagnostaras, et al., 1999). Impairments in the ability to generate new memories (anterograde amnesia) also occurs following hippocampal damage (Clark, et al., 2002) or disruption of synaptic plasticity within the hippocampus at the time of learning (Morris, et al., 1986; Young, et al., 1994).

This set of findings suggests that the hippocampus is not only critical for the initial formation and storage of memories, but also participates in their ability to be gradually stabilized in other brain regions, a process referred to as systems consolidation, which can take weeks, months, or even years (Squire, 2004). Importantly, systems consolidation is distinct from cellular consolidation (Debiec, et al., 2002), which provides a mechanism for the transition from active short-term memory to passive long-term memory in a process that occurs for several hours after the acquisition or retrieval of information (Schafe, et al., 1999; Nader, et al., 2000).

Initially it was thought that the magnitude of anterograde amnesia and retrograde amnesia was proportional (Squire & Alvarez, 1995). However, studies using contextual fear conditioning in rodents have shown that while retrograde amnesia is invariably pronounced, anterograde amnesia can be readily overcome with modest increases in training parameters (Maren, et al., 1997; Frankland, et al., 1998; Wiltgen, et al., 2006). This has led to the view that while the hippocampus is normally used for contextual fear conditioning, in the absence of the hippocampus other regions may compensate, albeit less efficiently (Fanselow, 2010).

Although contextual fear memories can be formed in the absence of the hippocampus, it is not clear whether memories formed using alternate circuitry maintain the same properties as those formed with the hippocampus intact. One classic hallmark of normal fear memories is that they last the lifespan of the rat without diminution (Gale, et al., 2004). However, the durability of contextual fear memories acquired in the absence of the hippocampus remains unknown. Ascertaining whether such memories retain permanency would provide insight into the brain mechanisms underlying compensation, the necessity of the hippocampus in systems consolidation and the optimization of targeted clinical approaches for the treatment of memory disorders.

We therefore tested whether contextual fear memories formed following damage to the dorsal hippocampus were as stable as those formed with the hippocampus intact. We discovered that rats conditioned in the absence of the dorsal hippocampus were able to acquire contextual fear and retain this memory across a one-day retention interval; however, as this interval was extended contextual fear expression began to fade. Thus, memories formed using compensatory structures may retain the ability to undergo cellular consolidation but lack the ability to undergo the consolidation process necessary to allow expression of these memories at remote time points.

Materials and Methods

Subjects

The subjects were 60 naïve, adult male Long-Evans rats, initially weighing 270–300 g, purchased from Harlan (Indianapolis, IN). Rats were individually housed and maintained on a 12-hour light/dark cycle with access to food and water ad libitum (all behavioral testing conducted during the light portion of the cycle). Animals were handled daily (one-two minutes per rat) for at least one week prior to the start of surgery and behavioral training. The procedures used in this experiment were in accordance with policy set and approved by the Institutional Animal Care and Use Committee of the University of California, Los Angeles.

Surgery

Rats were anesthetized with sodium pentobarbital (i.p., 65 mg/kg) and medicated with atropine sulphate (i.p., 0.4 mg/kg). Each rat was shaved across the head and their eyes were coated with a hydrating ointment. Animals were mounted into stereotaxic instruments (Kopf Instruments, Tujunga, CA) and the scalp was cleaned (70% ethyl alcohol and Betadine), incised, and retracted. The skull was adjusted so that bregma and lambda were in the same horizontal plane. Four small holes (two per side) were drilled into the skull to allow for a stainless steel injector cannula (33 gauge) aimed at the dorsal hippocampus to be positioned 2.8 mm posterior, 1.6 mm lateral, 3.5 mm ventral to bregma (rostral coordinates) and 4.2 mm posterior, 2.6 lateral, and 3.5mm ventral to bregma (caudal coordinates). Injection cannulae (33 gauge) were attached to a 5 μl microsyringe (Hamilton Instruments) via polyethylene tubing (PE20) and inserted into guide cannulae (28 gauge) attached to the arms of the stereotax. Microsyringes were mounted into a syringe pump (Harvard Apparatus, South Natick, MA) for controlled microinfusions of NMDA (20 mg/ml; Sigma-Aldrich, St. Louis, MO), dissolved in 0.01 M phosphate buffered saline (PBS). Cannulae were lowered and infusions of .4 μl of NMDA/site were made across 4 min (.1 μl/min rate). Cannulae remained in place for an additional two minutes to allow for adequate NMDA diffusion and reduction of backflow. Sham surgeries were identical except that injection cannulae were not lowered and infusions were not made. Following infusions, incisions were closed with stainless steel wound clips and animals were given i.p. injections of the analgesic/anti-inflammatory ketoprofen (2 mg/kg) and placed on heating pads until they recovered from anesthesia. Ketoprofen injections were continued for an additional two days post-surgery. In addition, rats were given the antibiotic trimethoprim sulfa (TMS) in their drinking water, weighed, monitored and handled for one-week following surgery. Rats were allowed a total of 11–13 days of recovery prior to behavioral training.

Apparatus

All behavioral training was performed in the same “context,” which was comprised of a set of four identical fear conditioning chambers (30 X 25 X 25 cm, Med-Associates, Inc St. Albans, VT), equipped with a Med-Associates VideoFreeze system. Individual boxes were enclosed in sound-attenuating chambers (Med-Associates) and the set was contained in an individual, dedicated experimental room, which provided a unique spatial location. Each box had aluminum sidewalls and a Plexiglas rear wall with blue dots. The grid floor consisted of 16 stainless steel rods (4.8 mm thick) spaced 1.6 cm apart (center to center; Contextual Conditioning System, Med-Associates, Inc.). Pans underlying each box were sprayed with a thin film of Simple Green® to provide the context with a scent. Chambers were individually lit from above with white house lights and cleaned with 70% isopropyl alcohol in between squads. Individual fans mounted above each chamber were turned on to provide background noise (60dB). The experimental room in which chambers were located was brightly lit with overhead lights. Animals were transported to chambers in squads of 4 in their homecages, which were slid onto hanging racks mounted to a portable cart and covered with a white sheet. Chambers were cleaned with a 10% bleach solution following each day of behavioral testing.

Fear conditioning

Ten to fourteen days following surgery (recovery period), rats were transported to the conditioning context in squads of four and placed into individual experimental chambers. Following a 180 second baseline period of exposure to the context, rats were fear conditioned using four, .9mA, 2 sec footshocks. Footshocks were separated by an 88 second inter-trial-interval (ITI). Following the final shock, rats were given 60 additional seconds in the context prior to being transported back to the vivarium. Rats were then brought back to the context for an eight-minute exposure session to test for contextual fear memory either one, three, ten or thirty days later (see Fig. 2A). This resulted in the following 8 experimental groups of a 2 (surgery) X 4 (retention interval) factorial design: Sham-1, Sham-3, Sham-10, Sham-30, DH-1, DH-3, DH-10, DH-30.

Figure 2
Longevity of a contextual fear memory formed in the absence of the hippocampus. A, Experimental design. B, Contextual fear acquisition. Mean (± sem) freezing to the context during the 30 second period preceding each footshock (trial) for rats ...

Histology

Following behavioral testing, animals were anesthetized and transcardially perfused with phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA). Brains were extracted and placed in 4% PFA overnight. The following day they were cryoprotected in a 30% sucrose solution for 72 hours. The brains were then frozen (−20° C) and sectioned on a cryostat (50 μm). Every third section (150 μm) was collected and dry mounted on a microscope slide. Sections were then stained for nissl bodies (cresyl violet), lesions were verified and images were captured using brightfield microscopy.

Data Analysis

Rats freeze in fear provoking contexts and we relied on this freezing behavior to assess fear memory. To do this we used an automated near infrared (NIR) video tracking equipment and computer software (VideoFreeze, Med-Associates Inc.). Video was recorded at 30 frames per second and the software calculated the frame-to-frame change in grayscale values for each pixel and summed these values for a frame. This value was compared to change values calculated when no animal was present (noise) to obtain an “activity score” for each frame. Based on previous validation with hand scoring by trained human observers that defined freezing as the absence of all movement except that necessitated by respiration (correlation of r >0.9 between automated system and highly trained human observers) an instance of freezing was defined as an activity score below 50 for longer than 1 sec. For fear acquisition, average freezing across the 30 seconds prior to each footshock administration was scored and analyzed. For fear memory at test, freezing across the entire eight minutes of context exposure was scored and analyzed. Freezing data were statistically analyzed using between-subjects analyses of variance (ANOVAs) and repeated measures (trial) ANOVAs where appropriate. Post-hoc tests (Bonferroni) were performed following significant findings. The level of significance used for all analyses was p < .05.

Results

To investigate the durability of memories formed in the absence of the dorsal hippocampus, rats were given excitotoxic lesions of the dorsal hippocampus or sham surgery (controls) prior to context fear conditioning. We focused specifically on the dorsal as opposed to ventral region of the hippocampus because of the dorsal hippocampus’ role in memory generally and in contextual fear in particular (Kim & Fanselow, 1992; Fanselow & Dong, 2010). Moreover, lesions of the dorsal hippocampus produce the same pattern of results for contextual fear memory as those produced by lesions of the entire hippocampal complex (Wiltgen, et al., 2006). We compromised the DH using permanent lesions as opposed to temporary inactivations because compensatory contextual fear memories have been demonstrated following the former (Maren, et al., 1997; Wiltgen, et al., 2006) but not the later (Sanders & Fanselow, 2003; Matus-Amat, et al., 2004). Moreover, inactivation of the DH has been shown to work well when one is targeting fear acquisition but does not affect consolidation (Kim, et al., 1991).

Representative photomicrographs of a DH-lesioned rat compared to sham control are displayed in Figure 1. The extent of NMDA-induced excitotoxic lesions of the DH was consistent with those previously reported in our laboratory (e.g., Quinn, et al., 2008). Rats with insufficient bilateral damage restricted to the DH were not included. Based on this criterion, 2 animals were excluded. This resulted in the following group sizes: Sham-1: n=8, Sham-3: n=6, Sham-10: n=8, Sham-30: n=8, DH-1: n=7, DH-3: n=6, DH-10: n=8, DH-30: n=7.

Figure 1
Representative photomicrographs show cresyl violet-stained coronal brain sections following excitotoxic lesions of the dorsal hippocampus (right) compared to sham controls (left). From top to bottom, the sections are 1.60, 2.60, 3.60, and 4.60 mm posterior ...

Following pre-training lesions of the DH or sham surgery, animals were allowed to recover for 11–13 days before undergoing behavioral testing. All rats underwent contextual fear acquisition and testing (see Fig. 2A for design). Figure 2B displays freezing across the acquisition session in sham and DH-lesioned rats. A repeated measures (trial) X lesion ANOVA revealed that both the sham and lesion groups were able to acquire contextual fear across conditioning trials (F(3,168) = 276.7, p < .0001) with an interaction between trial and lesion condition (F (3,168), = 3.34, p <.05).

Consistent with previous findings, rats showed a deficit in contextual fear expression following a single trial of conditioning (trial 2: t = 3.23; p < .01). However, this deficit disappeared with additional trials, consistent with the idea that contextual fear can be acquired in the absence of the hippocampus but less efficiently (Wiltgen, et al., 2006). There was no effect of lesion prior to any shock administration (trial 1: t = 0.04; p > .05), demonstrating that hippocampal lesions themselves did not affect baseline freezing.

Animals were tested for contextual fear memory one, three, ten or thirty days following fear acquisition (Fig. 2C). We chose one day as the moderate retention interval as it is well outside the period of cellular consolidation and is commonly thought to be an interval appropriate for testing “recent” long-term memory (Schafe, et al., 1999). To test for a possible failure in the retention of these memories, we examined contextual fear following a thirty-day retention interval, as retrograde amnesia studies suggest that this is a period over which systems consolidation occurs (Kim & Fanselow, 1992). Additional three-day and ten-day retention intervals were used to examine whether changes in fear expression across time were graded.

A two-way (lesion X retention interval) ANOVA revealed an overall significant effect of lesion, retention interval and an interaction of these factors (lesion: F(1, 50) = 86.29; p <.0001; interval: F(3, 50) = 14.90; p <.0001; interaction: F(3, 50) = 7.31; p <.001). Contextual fear expression following a one-day retention interval was not different between sham and DH-lesioned animals, demonstrating that both groups were able to form a long-term contextual fear memory (t = 1.52; p > .05). These findings are consistent with those previously reported (Wiltgen, et al., 2006).

However, when this retention interval was extended, the ability for DH-lesioned animals to maintain a level of fear that was comparable to controls was dramatically reduced (3-day: t = 3.20; p < .01; 10-day: t = 6.57; p < .001; 30-day: t = 7.45; p < .001). Moreover, there was a significant simple main effect of retention interval for rats with DH lesions but not for sham controls (DH: F (3, 27) = 13.98; p <.0001; sham: F (3, 28) = 1.42; p > .05), indicating that the loss of contextual fear expression across time was specific to DH-lesioned animals. These results indicate that expression of contextual fear memories formed in the absence of the hippocampus is attenuated with the passage of time.

Discussion

In this study, we found that rodents that acquire contextual fear memories in the absence of the dorsal hippocampus fail to express these memories as they become remote. These data suggest that contextual fear memories formed in the absence of the dorsal hippocampus may lack the persistence of those formed with the hippocampus intact. In line with previous findings, they show that animals can overcome the amnestic effects of pre-training DH lesions provided adequate training is given (Wiltgen, et al., 2006). However, our results are the first to reveal that the ability to overcome anterograde amnesia may be dependent on the length of time between training and test, such that recently acquired contextual fear memories remain intact while the expression of such memories at remote time points is dramatically impaired. Thus, explicit memories formed using alternate, compensatory structures seem to lack the permanence characteristic of normal contextual fear memories (Gale, et al., 2004). One way to interpret these findings is that contextual fear memories formed in the absence of the DH are unable to be consolidated in the same way as those formed with the DH intact.

These findings suggest that even when contextual fear memories rely on regions outside the DH for initial memory formation, the hippocampus is vital for the transformation of these memories from a recent to remote state. That is, the DH may be required for the long-term consolidation of explicit memories, regardless of the structure in which these memories were initially established. Thus, the properties required of an explicit memory in order for it to be subject to cellular consolidation may not necessarily be sufficient for that memory to be permanently consolidated.

We demonstrate that in order for a contextual fear memory to become permanent the DH must be intact. This implies that the compensatory structures recruited in the absence of the DH are incapable of permanently holding onto these memories, suggesting that they are more fragile in nature. The involvement of the DH in the ability for long-term memories to be stored permanently is supported by studies showing that memories formed in the remote past can be disrupted by blocking CA3 output, therein disturbing the integrity of the tri-synaptic pathway and the ripple-associated reactivation of hippocampal memory (Nakashiba, et al., 2009). Additionally, it has been shown that the prefrontal cortex is phase-locked to hippocampal theta (Siapas, et al., 2005) and that ablation of NMDARs on interneurons (Korotkova, et al., 2010) or blockade of electrical communication between interneurons in the hippocampus (Bissiere, et al., 2011) disrupts hippocampal synchrony and hippocampus-dependent memory. These findings support the idea that an intact hippocampus is essential for the permanent consolidation of a memory or at the least, that memories formed using alternate circuitry are not formed in neuronal ensembles that have access to the appropriate synchrony, electrical communication or intracellular signaling properties required for stable storage.

Surprisingly, the straightforward prediction that memories formed in the absence of the hippocampus lack permanence has never been tested. Two factors likely contribute to the difficulty in conducting such a test. One is that as the retention interval between training and testing increases, normal forgetting in the intact controls can obscure a failure of systems consolidation with hippocampal damage (Zola-Morgan & Squire, 1990). A second issue is that tasks that take several days to acquire do not allow a clear separation between the period of acquisition and systems consolidation. Thus, an ideal task to test for the longevity of memories is one that can both be acquired in a single brief session and is stable across time. By using contextual fear conditioning, we were able to employ a task that satisfied both these requirements.

Lastly, our findings dovetail nicely with those previously reported examining contextual fear in the absence of the basolateral amygdala (BLA). That is, the BLA is integral to fear learning and memory (Fanselow & LeDoux, 1999) and damage to the BLA results in a massive deficit in fear acquisition and expression (Maren, et al., 1996). However, animals are able to learn and express context fear following BLA damage, provided they are trained using a very robust acquisition protocol (Maren, 1999; Ponnusamy, et al., 2007; Poulos, et al., 2010). In addition, contextual fear memories formed in the absence of the BLA do not persist across time (Poulos, et al., 2009). Thus, our results for the DH parallel those found for the BLA, suggesting that both the DH and BLA are essential components of the circuitry required for a contextual fear memory to become permanent.

These findings imply a general conclusion about compensation following brain damage: namely, that learning and memory can occur in the absence of the relevant primary structure(s), but that learning is less efficient and that the memories formed decay with time. Therefore, when the primary circuit for a specific task is compromised, not only does it seem to be a general property of the brain that an alternate circuit be recruited to compensate (Fanselow, 2010), but it may also be a general finding that these compensatory memories fade with time. While further experiments examining whether the same pattern is maintained across different hippocampus-dependent tasks and preparations are warranted, the question is most relevant in situations where expression of memory remains unabated with time.

These results also have important clinical implications. They suggest that memories normally thought to involve the hippocampus may be formed following hippocampal damage, but that these memories are more fragile, failing to persist across time. Thus, clinical approaches towards reducing or overcoming amnesia could benefit from developing methods to promote the longevity of memories formed following brain damage. Such advances would be facilitated by the identification of the brain regions underlying contextual memories formed in the absence of the hippocampus.

Acknowledgments

This research was supported by National Institute of Mental Health grant RO1 MH062122 (M.S.F.) and a Charles F. Scott Endowed Fellowship (M.Z.). The authors would like to thank Timothy Hast and Rebecca Bennett for technical support.

Footnotes

Author contributions: M.Z. and M.S.F designed research. M.Z. performed research and analyzed data. M.Z., S.B., and M.S.F. discussed, wrote and revised the paper.

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