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BMC Cancer. 2012; 12: 48.
Published online Jan 28, 2012. doi:  10.1186/1471-2407-12-48
PMCID: PMC3299592
Cancer-initiating cells derived from established cervical cell lines exhibit stem-cell markers and increased radioresistance
Jacqueline López,1 Adela Poitevin,2 Veverly Mendoza-Martínez,4 Carlos Pérez-Plasencia,3 and Alejandro García-Carrancácorresponding author4
1Programa de Doctorado en Ciencias Bioquímicas, Facultad de Química, Universidad Nacional Autónoma de México, Mexico City, Mexico
2Hospital Médica Sur, Unidad de Radioterapia, Mexico City, Mexico
3Laboratorio de Oncología Genómica, Instituto Nacional de Cancerología & UBIMED-FESI, Universidad Nacional Autónoma de México, Mexico City, Mexico
4Unidad de Investigación Biomédica en Cáncer, División de Investigación Básica, Instituto Nacional de Cancerología, Secretaría de Salud & Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico
corresponding authorCorresponding author.
Jacqueline López: jlopezg/at/unal.edu.co; Adela Poitevin: mpoitevinc/at/medicasur.org.mx; Veverly Mendoza-Martínez: vevely/at/hotmail.com; Carlos Pérez-Plasencia: car_plas/at/yahoo.com; Alejandro García-Carrancá: carranca/at/biomedicas.unam.mx
Received June 27, 2011; Accepted January 28, 2012.
Abstract
Background
Cancer-initiating cells (CICs) are proposed to be responsible for the generation of metastasis and resistance to therapy. Accumulating evidences indicates CICs are found among different human cancers and cell lines derived from them. Few studies address the characteristics of CICs in cervical cancer. We identify biological features of CICs from four of the best-know human cell lines from uterine cervix tumors. (HeLa, SiHa, Ca Ski, C-4 I).
Methods
Cells were cultured as spheres under stem-cell conditions. Flow cytometry was used to detect expression of CD34, CD49f and CD133 antigens and Hoechst 33342 staining to identify side population (SP). Magnetic and fluorescence-activated cell sorting was applied to enrich and purify populations used to evaluate tumorigenicity in nude mice. cDNA microarray analysis and in vitro radioresistance assay were carried out under standard conditions.
Results
CICs, enriched as spheroids, were capable to generate reproducible tumor phenotypes in nu-nu mice and serial propagation. Injection of 1 × 103 dissociated spheroid cells induced tumors in the majority of animals, whereas injection of 1 × 105 monolayer cells remained nontumorigenic. Sphere-derived CICs expressed CD49f surface marker. Gene profiling analysis of HeLa and SiHa spheroid cells showed up-regulation of CICs markers characteristic of the female reproductive system. Importantly, epithelial to mesenchymal (EMT) transition-associated markers were found highly expressed in spheroid cells. More importantly, gene expression analysis indicated that genes required for radioresistance were also up-regulated, including components of the double-strand break (DSB) DNA repair machinery and the metabolism of reactive oxygen species (ROS). Dose-dependent radiation assay indicated indeed that CICs-enriched populations exhibit an increased resistance to ionizing radiation (IR).
Conclusions
We characterized a self-renewing subpopulation of CICs found among four well known human cancer-derived cell lines (HeLa, SiHa, Ca Ski and C-4 I) and found that they express characteristic markers of stem cell, EMT and radioresistance. The fact that CICs demonstrated a higher degree of resistance to radiation than differentiated cells suggests that specific detection and targeting of CICs could be highly valuable for the therapy of tumors from the uterine cervix.
Keywords: Cancer-initiating cells, Cervical cancer, Stem cell markers, Radioresistance, Epithelia to mesenchymal transition
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