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Published online 2011 December 13. doi: 10.1186/1471-2334-11-338

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Phylogenetic analysis of a 443-nucleotide region of rrs for 118 isolates of 14 Leptospira species. The tree was reconstructed using the neighbor-joining method as implemented in MEGA ver 5.0 [15]. Colour coding represents the following: RED, sequence of a PCR fragment amplified from patients with culture-positive leptospirosis; BLUE, sequence of a PCR fragment amplified from patients with culture-negative leptospirosis; YELLOW, sequence for reference strains obtained from GenBank.

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