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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Neurosci. Author manuscript; available in PMC 2012 August 15.
Published in final edited form as:
PMCID: PMC3297021

Kcna1 gene deletion lowers the behavioral sensitivity of mice to small changes in sound location and increases asynchronous brainstem auditory evoked potentials, but does not affect hearing thresholds


Sound localization along the azimuth depends on the sensitivity of binaural nuclei in the auditory brainstem to small differences in interaural level and timing occurring within a sub-millisecond epoch, and on monaural pathways that transmit level and timing cues with high temporal fidelity to insure their coincident arrival at the binaural targets. The soma and axons of these brainstem neurons are heavily invested with ion channels containing the low-threshold potassium channel subunit Kv1.1, which previous in-vitro and in-vivo studies suggest are important for regulating their high input-output correspondence and temporal synchrony. We compared awake Kcna1 null mutant (−/−) mice lacking Kv1.1 with +/+ mice to determine if Kv1.1 activity contributes to sound localization, and examined anesthetized mice for absolute hearing thresholds for suprathreshold differences that may be revealed in the waveforms of auditory brainstem response potentials. The awake −/− mice tested with reflex modification audiometry had reduced sensitivity to an abrupt change in the location of a broad band noise compared to +/+ mice, while anesthetized −/− mice had normal absolute thresholds for tone pips but a high level of stimulus-evoked but asynchronous background activity. Evoked potential waveforms had progressively earlier peaks and troughs in −/− mice but the amplitude excursions between adjacent features were identical in the two groups. Their greater excitability and asynchrony in suprathreshold evoked potentials coupled with their normal thresholds suggests that a disruption in central neural processing in −/− mice and not peripheral hearing loss is responsible for their poor sound localization.


The ability to locate sounds contributes to spatial orientation and navigation, and also helps humans and other animals segregate overlapping auditory signals, including vocalizations, by their perceived locations (Cherry, 1953; Feng & Ratnam, 2000). The major cues for sound localization are interaural time and intensity differences (ITD and IID) produced by small variations in the distance between a source and each ear and by sound-shadowing of the longer path around the head. Typical detection thresholds in mammals are less than 10° of arc and just 1° for humans (Hershkowitz & Durlach, 1969), indicating their utilization of ITDs of 10 μs and IIDs of 1 dB (Mills, 1958). High temporal fidelity of neurotransmission is required for both binaural comparisons, because these IID- and ITD-coding nuclei must integrate only near-coincident neural impulses arriving from each ear (Oertel, 1999; Joris & Yin, 1995). Anatomical and physiological specializations in the brainstem ensure that monaural neurons rapidly transmit their separate inputs in synchrony to their upstream targets and that binaural neurons have brief integration windows (Trussell 1999; Yin, 2002). One significant molecular adaptation in these neurons and in other brainstem nuclei involved in coincidence detection (e.g., octopus cells, Oertel et al., 2000) is their expression of voltage-sensitive Kv1 channels (Grigg et al., 2000; Rosenberger et al., 2003). The most sensitive subunit in these channels is Kv1.1, which is the focus of the present report.

In-vitro slice studies of a major monaural nucleus in the IID pathway, the medial nucleus of the trapezoid body (MNTB), show that cells from Kcna1 −/− mice lacking Kv1.1 respond with many poorly timed responses to current injection (high “jitter') while +/+ cells have single onset spikes with stable latencies (Brew et al., 2003; Gittelman & Tempel, 2006). Comparable in-vivo studies in anesthetized −/− mice report not hyper- but hypoexcitability to tone pips, though −/− cells in the cochlear nucleus (CN) and MNTB again show greater jitter (Kopp-Scheinpflug et al., 2003). More jitter was found also in −/− cells of the binaural lateral superior olivary nucleus (LSO) and their sensitivity was restricted to positive IIDs (Karcz et al., 2011). Related work (Karcz, 2011) found that LSO targets in the −/− Inferior Colliculus (IC) had normal jitter and sensitivity to both positive and negative IIDs, but greater sensitivity to IIDs outside the normal temporal integration window. The in-vivo studies also reported at least trends for higher response thresholds in −/− cells (significant in LSO and IC), this not being evident in in-vitro studies.

These in-vivo reports suggest a direct contribution of Kv1.1 to the neural processing of auditory space, though an alternative hypothesis is that Kcna1 deletion simply causes peripheral hearing loss. Here we report that awake −/− mice were indeed less sensitive to changes in sound location in a behavioral task, while anesthetized −/− mice had normal auditory brainstem response thresholds, but greater levels of background asynchrony. These data support the conclusion that a disruption in neural processing and not peripheral hearing loss is responsible for their poor sound localization.

Materials and Methods


Mice were of the C3HeB/FeJ-Kcna1tm1Tem strain (Smart et al., 1998), bred in the University of Rochester vivarium from heterozygotic (+/−) breeding pairs obtained from Jackson Laboratory (Bar Harbor, ME). Genotyping was performed on DNA isolated from tail clips of each mouse, following standard procedures described in protocols available on line ( A total of 63 mice of either sex were tested: 23 in the behavioral experiment (6M, 7F +/+, 7M, 3F −/−) and 48 in the auditory brainstem response (ABR) experiment (14M, 9F, 1U +/+, 12M, 10F, 2U −/−) with 8 mice tested in both experiments. The ABR was administered when the mice were on average 21 days of age, the behavioral tests began on average at 30 days of age. The behavioral experiment was carried out over a period of 7 months and the mice were selected from the available +/+ and −/− mice in that period of time. The ABR data were collected over a period of 15 months, and in order to minimize any possible litter effects, the groups here were specially selected and comprised of all of the matched +/+ and −/− littermates from the larger colony. The University of Rochester Committee on Animal Resources approved all procedures, which were in accord with USPHS regulations and the Federal Animal Welfare Act.

Auditory Brainstem Response

Mice were anaesthetized with ketamine/xylazine (120 mg/kg and 10 mg/kg) then placed on a 10-cm high platform inside a small (57L × 41W × 36H cm), electrically shielded, soundproofed booth (IAC, Inc., Bronx, NY) lined with 4.5 cm-thick echo-attenuating acoustic foam (Sonex®; Illbruck Acoustic, Minneapolis, MN). The mouse was arranged in a prone position facing a broad-band electrostatic loudspeaker system (TDT ES1 with ED1 speaker driver) located 10 cm from the head, and was kept warm during the procedure with a circulating-water heating pad. Miniature subdermal needle electrodes (Nicolet) were inserted at the vertex (reference electrode), over the bulla (active electrode), and just above the hind limb (ground electrode). Calibrated tone bursts (5 ms duration, 0.5 ms rise-fall time, phase alternating 90°) were synthesized with SigGenRP© software on a TDT RP2.1 real-time processor, and presented at a rate of 10/s at frequencies of 3, 6, 12, 16, 24, 32, and 48 kHz. The software included an artifact detector that rejected outlier responses. Average ABR waveforms (150 repetitions × 2 duplicates) were recorded at each tested frequency/amplitude combination and filtered from 3 Hz to 1000 Hz. Each 10 ms waveform was comprised of 500 points (0.02 ms sample period). Every wave form was normalized with a DC correction so that the sum of the 500 points was 0 μVolts. At each frequency, the amplitude of the signal was automatically attenuated (TDT PA5) in 5 dB steps between samples from 90 dB SPL (but 80 dB SPL maximum at 48 kHz) until the wave IV–V complex of the ABR waveform was no longer visually distinguishable from background in duplicate traces in the judgment of a highly experienced technician. By convention, threshold was established as the response at 5 dB above this final level. Suprathreshold amplitude measures were later analyzed from these waveforms, and are reported here for the 16 kHz test frequency presented at 80 dB SPL.

Behavioral measure of auditory spatial acuity

The auditory spatial acuity of mice was determined using Reflex Modification Audiometry as previously described (Allen & Ison, 2010). Briefly, a broadband noise was switched abruptly between speakers that were separated by 7.5°, 22.5°, 45 °, 90 ° and 180° in the frontal plane of the mouse (this switch being the prepulse, PP), with the order of the 5 angular separations across test days counterbalanced across mice. The mouse's detection of the PP was demonstrated in the inhibition (PPI) of the acoustic startle response (ASR) to a noise burst probe (the reflex eliciting stimulus, ES) when the probe was presented shortly after the change in the location of the background noise (a prepulse trial), compared to the response amplitude when it was presented without any prior change in the background noise (a control trial). A schematic diagram of the stimulus arrangement is provided in Figure 1.

Figure 1
Schematic description of the behavioral experiment. Two speakers were located 1 m from the mouse, and positioned in the azimuthal plane symmetrically around the midline with angular separations from 7° to 180°. The speaker presenting the ...

The experiment was conducted within a large (2.7L, 1.8W, 2.4H m) sound-attenuating IAC room with echo-attenuating Sonex acoustical foam lining the walls. The ES was generated using a Tucker-Davis Technology (TDT, Alachua, FL) RP2.1 Real-time Processor amplified with an Adcom (East Brunswick, NJ) GFA-535 II amplifier and broadcast via a Yamaha JA4281B compression tweeter. This stimulus was 110 dB SPL, but at just 20 ms duration its subjective level for a human listener approximated that of a hand clap at a distance of 30 cm. The background noise stimulus at 70 dB SPL was digitally generated using a second RP2 and broadcast from one of two matched TDT-ES1 electrostatic speakers located 50 cm from the mouse's head in the azimuthal plane. The force of the ASR was transduced by an accelerometer and its amplitude was scored as the integrated RMS voltage of this signal for 100 ms after the delivery of the ES. The experimental stimuli were controlled and the responses recorded by a PC using a custom LabView (National Instruments) front-end. On each test day there were 11 presentations of each PP condition, and 22 control startle responses. Prepulse stimuli were presented at 13 different interstimulus interval conditions (ISI = 1, 2, 5, 10, 20, 30, 40, 50, 60, 100, 150, 200, or 300 ms) prior to the startle stimulus in order to determine the peak time of their effect. The intertrial interval, during which the background noise was continuously present, averaged 20 s and was randomly selected from the range 15 to 25 s. Each testing session lasted approximately 60 minutes, with one session for each angular separation, each separated by at least one rest day. Scheduling constraints resulted in not all animals receiving all 5 stimulus conditions.

Data analysis

Prepulse inhibition scores were calculated as the ratio of the each subject's mean startle response amplitude in the prepulse condition (ASRp) compared with the no-prepulse control baseline (ASRc), PPI = 1 − [ASRp/ASRc]. Repeated-measures analyses of variance (ANOVA) with ISI duration as the within-subject variables were performed with SPSS v.18 (IBM-SPSS Inc, Somers NY), and genotype as well as angular separation were taken as between-subject variables: in fact angular separation was a within-subject variable, but because some animals did not complete all tests there would have been many missing values for a within-subject analysis of angular separation. For this reason it was treated as a between-subject variable, which provides a conservative estimate of its statistical significance. The p-values provided by the ANOVA were adjusted via the Hunyh-Feldt method for non-homogeneity of between-cell correlations. Graphical presentation of the data and supplemental t-tests on specific conditions within- and between-subjects used GraphPad Prism v4.2 (GraphPad Software Inc, La Jolla, CA).

ABR waveforms for the 16 kHz, 80 dB SPL stimulus condition were adjusted to equalize the mean amplitude of the first 0.5 ms portion of each waveform and the zero-time base was adjusted to align the latency of the first ABR peak (P1) at 0 ms. The morphology of the wave form was first analyzed with a series of mixed design ANOVA in 2 ms blocks beginning 2 ms prior to P1, with 99 time points as the within-subject variable and genotype as the between-subject variable. Given the significant interactions between genotype and time points in these analyses, a more detailed time analysis began with the mean difference between the +/+ and the −/−waveforms, and then examining the regions which exceeded the 95% confidence level for these differences.


Normal thresholds but more asynchronous auditory brainstem responses and reduced latencies in mice lacking Kv1.1

Auditory brainstem response thresholds were recorded as a measure of absolute threshold sensitivity for tone pip stimuli in the +/+ and −/− mice and also to examine possible differences in brainstem neural excitability and the timing of their suprathreshold ABR waveforms. Figure 2 describes these results. The threshold data shown in Figure 2A are typical for mice, with their best hearing at 16 kHz and thresholds rising steeply at lower frequencies, and more gradually for higher frequencies. The threshold differences between the two groups of mice were small: the ANOVA of these data provided a significant effect for test frequency, F(6/276) = 518.61, p < 0.001, ηp2 = 0.92, but the effect of the Kcna1 genotype was not significant (p > 0.10). The 95% confidence interval for the mean threshold difference across test frequencies ranged from 4.0 dB favoring the +/+ mice to 0.42 dB favoring the −/− mice.

Figure 2
Normal ABR thresholds and abnormal suprathreshold waveforms in Kcna1 −/− and Kcna1 +/+ mice (n = 24, 24). (A) Mean (SEM) thresholds for each test frequency, showing the significant (p = .001) U-shaped function typical of mice with their ...

Figure 2B shows the average waveforms in the two groups for 16 kHz at 80 dB after each individual waveform has been time- and amplitude-normalized as described above. Overall the mean ABR amplitude for the −/− mice was greater than that of the +/+ mice, t(46) = 2.53, p = 0.015, R2 = 0.12. A series of four ANOVA over 2 ms spans beginning 2 ms prior to P1 then continuing out to the 0 to 2 ms band, the 2 to 4 ms band, and the 4 to 6 ms band after P1 each provided significance (all p < 0.05) for interactions between latency trends and genotype, indicating that the amplitude difference between the groups was not constant across the waveform. Figure 2B includes the latency regions in which the difference between the groups exceeded the 95% confidence interval, which tend to coincide with the end of a trough and the beginning rise to the next peak.

Figure 2C depicts the mean (SEM) latency differences between +/+ and −/− mice for each peak and trough feature of the waveform. There was no difference in raw P1 latencies: +/+ 2.250 (0.024) ms; −/− 2.255 (0.017) ms. The difference between groups showing more rapid transmission in the −/− group was small but significant: for N1, t(46) = 3.10, p = 0.003, R2 = 0.17; for P2, t(46) = 3.30, p = 0.002, R2 = 0.19; for N3, t(46) = 2.33, p = 0.024, R2 = 0.11; and for P4, t(46) = 2.941, p = 0.005, R2 = 0.16. The ANOVA of the data of Figure 2C provided a highly significant main effect of waveform feature F(6/276) = 3369.89, p < 0.001, ηp2 = 0.99, and for the interaction of genotype and the linear trend for features, F(1/46) = 5.00, p = 0.03, ηp2 = 0.10. In short, the difference in ABR latencies between the genotypes is highly significant, and increases with increasing ABR feature number, indicating a cumulative process, leading to an increased disparity between the groups for later peaks/troughs.

Figure 2D shows mean (SEM) amplitude difference in peak-to-trough potentials between neighboring features, beginning with the (negative) change in amplitude from P1 to N1 and ending with the (positive) change in amplitude from N3 to P4. Here, despite the significant overall higher level of neural activity in the −/− mice, there are no differences apparent in the overall amplitude of the excursions between one feature and the next, the ANOVA providing F(1/46) = 0.25 and the interaction between genotype across features yielding F(5/230) = 1.21, p > 0.3. The mean difference overall between the two groups was 0.174 μV and the 95% confidence interval extended from +0.861 to −0.516 μV.

Impaired behavioral auditory spatial acuity in mice lacking Kv1.1

Auditory spatial acuity was impaired in Kcna1 −/− mice as illustrated in Figure 3A in the reduced PPI effect of a 45° location shift compared with +/+ controls. There was an inverted U-shaped trend in PPI for both groups as the interstimulus interval (ISI) between moving the noise from one position through a 45 degree angle to a second position and the ISI for the startle probe was varied from 5 to 300 ms (the 1 and 2 ms points equaled the control levels and for clarity are not included in this figure). The behavioral effect of this shift in location was lower in the null mutant mice: for the quadratic trend, F(1/18) = 44.3, p = 0.001, ηp2 = 0.71 This inverted U-pattern with a peak level of inhibition centered at about 50 ms was replicated at each angle save for 7.5°.

Figure 3
Impaired spatial acuity in Kcna1-null mutant mice. (A) Mean (SEM) PPI produced by the 45° change in sound location for the Kcna1 −/− (N=10) and +/+ control mice (N=13) for different time intervals (ISI) between the change in speaker ...

Figure 3B depicts how the peak PPI (mean of 40, 50, and 60 ms ISI) varied by angle for the two groups, and shows that compared to the +/+ group, performance in the −/− group more rapidly declined as the angular separation was reduced below 180°. The ANOVA of peak performance provided significant effects for genotype, F(1/85) = 14.22, p < 0.001, ηp2 = 0.43; for angular separation, F (4/85) = 13.67, p < 0.001, ηp2 = 0.31; and for the interaction of genotype and angular separation, F (4/85) = 3.96, p =0.005, ηp2 = 0.15. For the 180° separation the groups were not significantly different from each other and both provided significant levels of PPI, but the group differences were significant for the 90°, 45°, and 22.5° separations. Also, for these three angles the average behavioral effect for +/+ mice was always significantly above zero (p < 0.05) while for the −/− mice it was never significant, while at 7.5° neither group had significant PPI above zero. The major contribution of the 7.5° data is to show that any spectral difference that might be present between the two speakers was not sufficient to have a behavioral effect.

Smart et al. (1998) had remarked that Kcna1 −/− mice have an exaggerated ASR, but did not provide any supporting evidence for their observation. In the current experiment the +/+ mice had more vigorous responding, with a mean response (SD) of 1622 (533) aV-units compared to 893 (271) for the −/− mice: t(21) = 3.93, p = 0.001, R2 = 0.42. Less vigorous responding in Kcna1 −/− mice compared to the +/+ mice was reported also by Karcz (2011) especially for normal listening conditions, the difference between the groups being reduced when one ear was plugged.


Behavioral responses to changes in sound location

The hypothesis that the Kcna1 gene contributes to the ability of mice to localize sounds has its foundation in the in-vitro and in-vivo experiments reporting that the principal monaural and binaural neurons in the brainstem pathways that transmit and encode IID stimuli lose their high level of temporal synchrony between neural inputs and response outputs in Kcna1 −/− mice (Brew et al., 2003; Gittelman & Tempel, 2006; Kopp-Scheinpflug et al., 2003; Karcz et al., 2011). This hypothesis is further supported by recent in-vivo data showing that at the level of the midbrain IC the Kcna1 −/− mice are sensitive to temporally displaced inhibitory inputs that lie outside the normal temporal window for coincidence integration (Karcz, 2011). The behavioral experiment reported here also supported this hypothesis, in its showing that −/− were less able to detect changes in the location of a noise background over angular separations from the near localization threshold for mice at 22.5° (Heffner& Heffner, 1988; Allen & Ison, 2010), up to 45° and 90°. Two control measures anchored these results, with the widest separation of 180° showing that both groups detected these stimuli and at the lowest separation of 7.5° neither group detected them. Based on Chen et al. (1995), who reported interaural attenuations in the mouse, we estimate that an IID of about 35 dB (180°) was an effective stimulus in both Kcna1 +/+ and −/− mice, while an IID of 5 dB (22.5°) was effective for the +/+ mice, but not for the −/− mice.

Implications of the ABR data for understanding the behavioral results

One apparent difference in outcome between the in-vitro experiments that stimulate Kcna1 −/− cells with current injection (Brew et al., 2003; Gittelman & Tempel, 2006), and the in-vivo experiments that use sound stimulation (Kopp-Scheinpflug et al., 2003; Karcz et al., 2011; Karcz, 2011), is the in-vitro finding of lower response thresholds and hyperreactivity in the −/− cells, contrasted with the in-vivo finding of higher response thresholds and hyporeactivity in −/− mice. One hypothesis that would explain these differences in response thresholds is that the Kcna1 −/− mouse has a peripheral hearing deficit that affects their responding to tones but not to current injection, this being plausible given the normal high expression of this gene in the spiral ganglion cells of the auditory nerve (Adamson et al., 2002). Further, hearing loss might increase the temporal dispersion of onset responses to suprathreshold tonal stimuli as found in the in-vivo experiments, and also reduce their performance in the behavioral experiment, as this too depends in part on stimulus intensity (Allen and Ison, 2010). The evoked potential experiment in anesthetized mice was designed to test this hypothesis in anesthetized mice using the ABR test to compare the hearing thresholds of +/+ and −/− mice: as shown in Figure 2A, their thresholds from 3 kHz up to 48 kHz were close to identical. Alternative hypotheses to explain the difference between the in-vitro and in-vivo observations have been suggested (e.g., Kopp-Scheinpflug et al., 2003) that would apply to behavioral experiments as well: that cells studied in-vivo are affected by the possibly enhanced inhibitory inputs from other different cell types, and their excitability is also dependent on more peripheral axonal input which can be blocked by the greater occurrence of back-firing and collision in −/− cells (Chui et al. 1999).

The suprathreshold ABR waveform may help explain other effects of Kv1.1 deletion. The group average ABR waveforms depicted in Figure 2B mark the passage of peaks of synchronous depolarization and troughs of synchronous repolarization and recovery of neural generators. These have been described for the mouse (Henry, 1979) and include the afferent pathway important for sound localization; the cochlear nucleus, the superior olivary complex, the nuclei and pathways of the lateral lemniscus that then access the midbrain nuclear groups of the inferior colliculus. Evident in Figure 2B is the presence of a significant level of stimulus evoked but non-synchronized activity in the −/− mice, that might be the evoked potential counterpart of the non-synchronized hyperactivity typical of in-vitro observations. Other than this shift in firing level, the topography of the +/+ and −/− waveforms were very similar (Figure 2D) save for the slightly more rapid transmission of synchronous activity beginning with the earlier return of depolarization (N1) that followed auditory nerve firing in −/− mice, and increasing on up through the brainstem. This is a classic example of the effect of blocking Kv1.1 subunits, because in the absence of this most sensitive mechanism for repolarizing the cell membrane, then the next round of depolarization culminating in the next peak must begin more quickly. We note however that the maximal latency difference between the two groups is just 0.2 ms and while this may be an interesting symptom of neural dysfunction, it seems doubtful that by itself it would substantially disrupt normal auditory processing, indeed auditory temporal processing at the several-millisecond time scale is intact in these mice as measured via gap detection (Allen et al., 2008). The likely more serious consequence of Kcna1 deletion seen in the ABR waveform is its allowing significant levels of asynchronous and “noisy” neural activity to occur in the brainstem, which could present a more serious challenge to the relatively normal processing of IID signals reflected in the similar amplitudes of the excursions from one waveform feature to the next.

Conclusions: The several auditory and non-auditory brainstem sites that contribute to sound localization which may be affected by Kcna1 deletion

The hypothesis that Kcna1 deletion in the mouse would reduce its sensitivity to changes in the location of a sound source was confirmed in the behavioral experiment, and the similar hearing thresholds obtained in the ABR experiment argues that Kcna1 had a direct effect on the behavior, rather than an indirect effect resulting from a peripheral hearing loss. The specific locus of the dysfunctional central neural sites affected by Kcna1 deletion cannot be determined at present, because in addition to the particular chain of monaural and binaural nuclei investigated in the electrophysiological experiments on which this hypothesis was based, there are other nuclei and other pathways where Kcna1 expression is found, and that contribute to sound localization.

The apparent spectral composition of complex stimuli varies depending on the relative positions of the sound and the listener because of the directional filtering properties of the head and ears (in humans, Shaw, 1972; cats, Rice et al. 1992), and this has been shown to provide a significant cue for monaural sound localization (in humans, Butler, 1986, and in cats, May, 2000). Karcz (2011) found that monauralized mice had reduced but still significant inhibition for shifts in azimuthal location over 90° and 120°. This monaural effect was also diminished by Kcna1 deletion. Electrophysiological correlates of spectral filtering have been found in the dorsal cochlear nucleus, the DCN (Spirou and Young, 1991), and Kv1.1 is known to be expressed in the DCN (Grigg et al. 2000), and can be expected to contribute to its processing complex spectral stimuli.

Both monaural spectral and binaural interaural IID and ITD cues to sound localization must fluctuate with bodily movements and for the mouse, with changes in the orientation of the pinna, as they do for the mobile pinna of the cat (Young et al., 1996). Accurate sound localization may require stabilization of acoustic space, e.g. via a vestibulo-auricular reflex (Tollin et al., 2008) in the same way that the vestibulo-ocular reflex stabilizes visual space (Lorente de No 1933). Movement generated fluctuations of the sound field must be distinguished from external changes in the position of sound sources, and this may be the province of the vestibular inputs expressing Kv1.1 (Kalluri et al., 2010) and similar somatosensory and proprioceptive inputs into the brainstem auditory nuclei (Shore and Zhou, 2006). These multisensory cells have been most actively investigated in the DCN (for example, Kanold et al. 2011), and while their functional significance is not fully understood, it is thought that DCN circuits and others, in the IC perhaps, must play a modulatory role in sound localization (Oertel and Young, 2004). To the extent that these circuits depend on the temporal coincidence of, for example, fluctuations in IID and proprioceptive and movement cues then it may be that they would also be disrupted by Kcna1 deletion. The investigation of these potential site-specific contributions of Kcna1 will depend on the development of site-specific changes in Kcna1 expression. Of particular of interest would be joint electrophysiological and behavioral investigations of these suggested cross-modal effects necessary for disambiguating external from internal influences sources on the proximal acoustic cues for sound localization.


This research was supported by USPHS NIH-NIA P01 Grant AG09524, NIH-NIDCD P30 Grant DC005409, and by the Schmitt Foundation for Integrative Brain Research. The authors thank Drs. Bruce Tempel, Cornelia Kopp-Scheinpflug and Gary Paige for discussion and comments on previous versions of this manuscript; Dr. Robert Frisina, Xiaoxia Zhu and Martha Zettel for genotyping the mice; Dr. William O'Neill, Dr. Joseph Walton, and John Housel for their contributions to ABR testing and interpretation; and two anonymous reviewers for their well-informed comments.


The authors have no conflicts of interest.


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