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J Bacteriol. Mar 2012; 194(6): 1639.
PMCID: PMC3294840
Draft Genome Sequence of Vibrio fischeri SR5, a Strain Isolated from the Light Organ of the Mediterranean Squid Sepiola robusta
Mattias C. Gyllborg,a Jason W. Sahl,b David C. Cronin, III,a David A. Rasko,b and Mark J. Mandelcorresponding authora
aDepartment of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA
bUniversity of Maryland School of Medicine, Institute for Genome Sciences, Department of Microbiology and Immunology, Baltimore, Maryland, USA
corresponding authorCorresponding author.
Address correspondence to Mark J. Mandel, m-mandel/at/northwestern.edu.
Received December 30, 2011; Accepted January 9, 2012.
Abstract
Here, we describe the draft genome sequence of Vibrio fischeri SR5, a squid symbiotic isolate from Sepiola robusta in the Mediterranean Sea. This 4.3-Mbp genome sequence represents the first V. fischeri genome from an S. robusta symbiont and the first from outside the Pacific Ocean.
Vibrio-squid mutualisms represent valuable models for the study of mechanisms that underlie the development and maintenance of specific microbe-host symbioses. The most-detailed studies have come from the specific association between the Hawaiian bobtail squid, Euprymna scolopes, and Vibrio fischeri (5, 8, 10). Here, we report the draft genome sequence of V. fischeri strain SR5 (3), which was isolated from the Mediterranean sepiolid squid, Sepiola robusta. S. robusta exhibits unique anatomical and behavioral features compared to E. scolopes (4), but some symbionts of S. robusta, including SR5, can also colonize E. scolopes efficiently, making them valuable isolates for mechanistic studies.
Paired-end 454 (2.5-kb inserts) and Illumina (300-bp inserts) libraries were constructed and sequenced to depths of approximately 30-fold (454 GS FLX Titanium) and 60-fold (Illumina GA II), respectively. Contigs were assembled with MIRA version 3.2.1.17 (1) and Seqman NGen 3.0 (DNASTAR, Madison, WI). The resulting 73 contigs represent a high-confidence draft sequence for the 4.3-Mbp V. fischeri SR5 genome, with an N50 of 490 kb. Contigs spanning the presumed chromosomal replication origins were split into two, and the resulting 75 contigs were oriented similarly to other sequenced V. fischeri genomes with Mauve Contig Mover (2, 7).
Mapping of the contigs against the genome of the monocentrid fish symbiont, V. fischeri MJ11 (6), suggested that the completed V. fischeri SR5 genome will be colinear with MJ11. From this analysis, SR5 contigs were assigned to chromosome I (n = 8 contigs, 2.8 Mbp), to chromosome II (n = 4 contigs, 1.3 Mbp), or as unmapped (n = 61 contigs, 0.1 Mbp). The large number of unmapped SR5 contigs are largely flanked by repetitive sequences, including rrn operon genes (rRNA/tRNA) and genes that are found in multicopy within the V. fischeri chromosome (e.g., RTX genes) and are thought to be difficult genomic regions rather than extrachromosomal DNA.
Analysis of orthologs (2, 6) among SR5, MJ11, and ES114 identified protein-coding genes unique to each sequenced V. fischeri strain. The SR5-specific genes are in small islets distributed across both chromosomes, typically consisting of 1 to 20 genes. The islets often encode members of phosphotransferase systems (PTS) and other metabolic genes, suggesting that this strain may encode novel and/or enhanced metabolic capacities that enable the expanded host range relative to MJ11. Absent from SR5 are the ES114-specific biofilm regulator rscS (9, 11) and the putative siderophore biosynthesis and receptor genes conserved between MJ11 and ES114 (VF_A0156 to VF_A0165). These observations suggest a number of intriguing issues for future studies as to what biological activities are required for squid colonization and how SR5 is able to efficiently colonize its natural host and the heterologous E. scolopes when MJ11 cannot.
Nucleotide sequence accession number.
The V. fischeri SR5 whole-genome shotgun project has been deposited in GenBank under the accession number AHIH00000000.
ACKNOWLEDGMENTS
We thank Egon Ozer for helpful discussions.
This work was supported by NSF IOS-0843633. J.W.S. and D.A.R. were supported by funds from the State of Maryland.
1. Chevreux B. 2005. MIRA: an automated genome and EST assembler. Ph.D. dissertation. German Cancer Research Center Heidelberg, Heidelberg, Germany.
2. Darling AE, Mau B, Perna NT. 2010. progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS One 5:e11147. [PMC free article] [PubMed]
3. Fidopiastis PM, von Boletzky S, Ruby EG. 1998. A new niche for Vibrio logei, the predominant light organ symbiont of squids in the genus Sepiola. J. Bacteriol. 180:59–64. [PMC free article] [PubMed]
4. Foster J, von Boletzky S, McFall-Ngai M. 2002. A comparison of the light organ development of Sepiola robusta Naef and Euprymna scolopes Berry (Cephalopoda: Sepiolidae). Bull. Marine Sci. 70:141–153.
5. Mandel MJ. 2010. Models and approaches to dissect host-symbiont specificity. Trends Microbiol. 18:504–511. [PubMed]
6. Mandel MJ, Wollenberg MS, Stabb EV, Visick KL, Ruby EG. 2009. A single regulatory gene is sufficient to alter bacterial host range. Nature 458:215–218. [PMC free article] [PubMed]
7. Rissman AI, et al. 2009. Reordering contigs of draft genomes using the Mauve aligner. Bioinformatics 25:2071–2073. [PMC free article] [PubMed]
8. Ruby EG, McFall-Ngai MJ. 1992. A squid that glows in the night: development of an animal-bacterial mutualism. J. Bacteriol. 174:4865–4870. [PMC free article] [PubMed]
9. Visick KL, Skoufos LM. 2001. Two-component sensor required for normal symbiotic colonization of Euprymna scolopes by Vibrio fischeri. J. Bacteriol. 183:835–842. [PMC free article] [PubMed]
10. Visick KL, Ruby EG. 2006. Vibrio fischeri and its host: it takes two to tango. Curr. Opin. Microbiol. 9:632–638. [PubMed]
11. Yip ES, Geszvain K, DeLoney-Marino CR, Visick KL. 2006. The symbiosis regulator rscS controls the syp gene locus, biofilm formation and symbiotic aggregation by Vibrio fischeri. Mol. Microbiol. 62:1586–1600. [PMC free article] [PubMed]
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