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AMB Express. 2012; 2: 9.
Published online Jan 26, 2012. doi:  10.1186/2191-0855-2-9
PMCID: PMC3293722
Identification of mosquito larvicidal bacterial strains isolated from north Sinai in Egypt
Ferial M Rashad,corresponding author1 Waleed D Saleh,1 M Nasr,2 and Hayam M Fathy1
1Department of Microbiology, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
2Department of Microbiology, National Center for Radiation Research and Technology, Nasr city 11371, Egypt
corresponding authorCorresponding author.
Ferial M Rashad: ferialrashad/at/yahoo.com; Waleed D Saleh: waleeddiaeddeen/at/yahoo.com; M Nasr: mohamednasr/at/yahoo.com; Hayam M Fathy: haya2000/at/maktoob.com
Received November 19, 2011; Accepted January 26, 2012.
Abstract
In the present study, two of the most toxic bacterial strains of Bacillus sphaericus against mosquito were identified with the most recent genetic techniques. The PCR product profiles indicated the presence of genes encoding Bin A, Bin B and Mtx1 in all analyzed strains; they are consistent with protein profiles. The preliminary bioinformatics analysis of the binary toxin genes sequence revealed that the open reading frames had high similarities when matched with nucleotides sequence in the database of other B. sphaericus strains. The biological activity of B. sphaericus strains varied according to growing medium, and cultivation time. The highest yield of viable counts, spores and larvicidal protein were attained after 5 days. Poly (P) medium achieved the highest yield of growth, sporulation, protein and larvicidal activity for all tested strains compared to the other tested media. The larvicidal protein produced by local strains (B. sphaericus EMCC 1931 and EMCC 1932) in P medium was more lethal against the 3rd instar larvae of Culex pipiens than that of reference strains (B. sphaericus 1593 and B. sphaericus 2297). The obtained results revealed that P medium was the most effective medium and will be used in future work in order to optimize large scale production of biocide by the locally isolated Bacillus sphaericus strains.
Keywords: Bacillus sphaericus, PCR, Sequencing, Conventional media, Culex pipiens, Larvicidal activity
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