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Logo of bmcmicrBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Microbiology
 
BMC Microbiol. 2012; 12: 19.
Published online Feb 1, 2012. doi:  10.1186/1471-2180-12-19
PMCID: PMC3293717
Chemotaxis of Burkholderia sp. Strain SJ98 towards chloronitroaromatic compounds that it can metabolise
Janmejay Pandey,#1,2 Narinder K Sharma,#1,3 Fazlurrahman Khan,1 Anuradha Ghosh,1,3 John G Oakeshott,4 Rakesh K Jain,1 and Gunjan Pandeycorresponding author4
1Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India
2Georgia Health Science University, Augusta GA 30912, USA
3Kansas State University, Manhattan, KS 66506, USA
4CSIRO Ecosystem Sciences, GPO Box 1700, Canberra ACT 2601, Australia
corresponding authorCorresponding author.
#Contributed equally.
Janmejay Pandey: jpandey/at/georgiahealth.edu; Narinder K Sharma: nksharma/at/ksu.edu; Fazlurrahman Khan: fazal/at/imtech.res.in; Anuradha Ghosh: anuradha/at/ksu.edu; John G Oakeshott: john.oakeshott/at/csiro.au; Rakesh K Jain: rkj/at/imtech.res.in; Gunjan Pandey: gunjan.pandey/at/csiro.au
Received July 21, 2011; Accepted February 1, 2012.
Abstract
Background
Burkholderia sp. strain SJ98 is known for its chemotaxis towards nitroaromatic compounds (NACs) that are either utilized as sole sources of carbon and energy or co-metabolized in the presence of alternative carbon sources. Here we test for the chemotaxis of this strain towards six chloro-nitroaromatic compounds (CNACs), namely 2-chloro-4-nitrophenol (2C4NP), 2-chloro-3-nitrophenol (2C3NP), 4-chloro-2-nitrophenol (4C2NP), 2-chloro-4-nitrobenzoate (2C4NB), 4-chloro-2-nitrobenzoate (4C2NB) and 5-chloro-2-nitrobenzoate (5C2NB), and examine its relationship to the degradation of such compounds.
Results
Strain SJ98 could mineralize 2C4NP, 4C2NB and 5C2NB, and co-metabolically transform 2C3NP and 2C4NB in the presence of an alternative carbon source, but was unable to transform 4C2NP under these conditions. Positive chemotaxis was only observed towards the five metabolically transformed CNACs. Moreover, the chemotaxis was induced by growth in the presence of the metabolisable CNAC. It was also competitively inhibited by the presence of nitroaromatic compounds (NACs) that it could metabolise but not by succinate or aspartate.
Conclusions
Burkholderia sp. strain SJ98 exhibits metabolic transformation of, and inducible chemotaxis towards CNACs. Its chemotactic responses towards these compounds are related to its previously demonstrated chemotaxis towards NACs that it can metabolise, but it is independently inducible from its chemotaxis towards succinate or aspartate.
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