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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Biometals. Author manuscript; available in PMC Mar 5, 2012.
Published in final edited form as:
PMCID: PMC3293496
NIHMSID: NIHMS359262
Genetics And Environmental Regulation Of Shigella Iron Transport Systems
Elizabeth E. Wyckoff, Megan L. Boulette, and Shelley M. Payne
Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712
Shigella spp . have transport systems for both ferric and ferrous iron. The iron can be taken up as free iron or complexed to a variety of carriers. All Shigella species have both the Feo and Sit systems for acquisition of ferrous iron, and all have at least one siderophore-mediated system for transport of ferric iron. Several of the transport systems, including Sit, Iuc/IutA (aerobactin synthesis and transport), Fec (ferric di-citrate uptake), and Shu (heme transport) are encoded within pathogenicity islands. The presence and the genomic locations of these islands vary considerably among the Shigella species, and even between isolates of the same species. The expression of the iron transport systems is influenced by the concentration of iron and by environmental conditions including the level of oxygen. ArcA and FNR regulate iron transport gene expression as a function of oxygen tension, with the sit and iuc promoters being highly expressed in aerobic conditions, while the feo ferrous iron transporter promoter is most active under anaerobic conditions. The effects of oxygen are also seen in infection of cultured cells by Shigella flexneri; the Sit and Iuc systems support plaque formation under aerobic conditions, whereas Feo allows plaque formation anaerobically.
The Shigella species are close genetic relatives of Escherichia coli and may be considered as subspecies, although they were historically categorized as a different genus and form a distinct group with respect to pathogenicity. These enteric pathogens are divided into four species (S. dysenteriae, S. flexneri, S. sonnei, S. boydii) based on serological and biochemical differences, but all the Shigella species are capable of invading human colonic epithelial cells, rapidly lysing the endocytic vesicle and multiplying intracellularly before moving directly into adjacent cells (Fig. 1). Shigellae are highly infectious, and the destruction of intestinal epithelial cells and resultant inflammatory response are responsible for the pathology of shigellosis, also termed bacillary dysentery (Sansonetti 2006).
Fig. 1
Fig. 1
Invasion and expression of iron transport genes in Shigella.
The intracellular environment inhabited by shigellae is quite different from that encountered outside the epithelial cell or outside the human host. Temperature, oxygen tension, nutrient availability and the form and amount of available iron are distinctly different in these environments. To better understand how Shigella acquires iron from its diverse environments, we have characterized the iron transport systems of Shigella spp. and measured their expression, regulation and function in vitro and in the eukaryotic cell cytoplasm.
The Shigella species have a common set of iron transport systems, with additional systems being present in some strains (Table 1). The systems that are common to all the strains tested are the ferrous iron transporters Sit and Feo (Runyen-Janecky et al. 2003). Sit, which has homology to a ferrous iron and manganese ABC transport system in Salmonella enterica Typhimurium (Janakiraman and Slauch 2000; Kehres et al. 2002), is found in all Shigella as well as enteroinvasive and some other pathogenic E. coli, but is not present in E. coli K12 or other non-pathogenic enterics (Runyen-Janecky et al. 2003). In contrast, the Feo transport system is widely distributed among bacteria, and the Shigella genes have a high degree of homology with the feoABC genes present in E. coli (Kammler et al. 1993). Given its ubiquity and ability to transport ferrous iron, the Feo system was likely the first iron transport system to arise in the ancestor of the enteric bacteria. The appearance of oxygen and, subsequently, ferric iron necessitated specific transporters for this less soluble form of iron.
Table 1
Table 1
Iron transport systems in Shigella speciesa
Shigella species can transport ferric iron using siderophore-mediated transport systems. These ferric transporters use a low-molecular-weight iron, high-affinity chelator that promotes the uptake of ferric iron, even at very low concentrations (Neilands 1984). The transport systems for the ferric siderophore include an outer membrane receptor, a periplasmic binding protein and a cytoplasmic membrane permease. Interestingly, there is no single siderophore that is common to all the Shigella spp. S. dysenteriae, S. sonnei and some strains of S. boydii produce enterobactin (Ent), the catechol type siderophore produced by most E. coli and Salmonella strains (Payne 1980; Payne et al. 1983; Perry and San Clemente 1979). S. dysenteriae type 1 also produces salmochelins, which are catechol-type siderophores related to enterobactin (Hantke et al. 2003; Payne et al. 2006). However, many S. flexneri and S. boydii have lost the ability to produce and use this compound. The genes are present within the genome, but point mutations, deletions and insertions have rendered the system inoperative in these pathogens (Schmitt and Payne 1988, 1991). Instead, these species produce aerobactin (Iuc), a hydroxamate siderophore that is transported by a distinct siderophore transport system (Iut) (Lawlor and Payne 1984; Payne 1980). Some Shigella isolates produce both siderophores (Payne et al. 2006). Although aerobactin production has been associated with virulence in some pathogenic E. coli, there does not appear to be an advantage of aerobactin over enterobactin production among Shigella strains. Both enterobactin producers and aerobactin producers are fully virulent, and they invade and grow within cultured epithelial cells in the laboratory with equal efficiency. All clinical isolates that we have tested produce at least one siderophore. Thus, siderophore production, but not the specific type of siderophore, appears to be important. In addition to their own siderophore, Shigella species, like other enterics, have a transport system for ferrichrome (Fhu), a fungal hydroxamate siderophore (Payne 1980).
Ferric iron can also be transported as ferric dicitrate. The fec locus has been identified in some strains of S. flexneri (Luck et al. 2001), and the genes are present in the sequenced genome of S. sonnei (ERIC database). S. dysenteriae type 1 has genes with homology to the E. coli fec genes, but two of the genes, fecR and fecB, are pseudogenes. Thus, a functional ferric dicitrate system is not commonly found in Shigella.
Heme, which is the most abundant iron source in the human host, is used by some, but not all Shigella spp. S. dysenteriae type 1 has an efficient heme transport system consisting of an outer membrane receptor (ShuA), a periplasmic binding protein (ShuT) and a cytoplasmic membrane permease (ShuUV) (Mills and Payne 1997; Wyckoff et al. 1998). ShuS, a cytoplasmic protein, is required for efficient use of heme at low heme concentrations and for protection against heme toxicity (Wyckoff et al. 2005). ShuW, X and Y are encoded within the shu locus, but their functions are unknown. No heme oxygenase or other protein for removing iron from the imported heme has yet been identified in Shigella. The shu genes are present in enterohaemorrhagic E. coli, in other pathogenic E. coli and in S. sonnei (Wyckoff et al. 1998). The heme transport locus in E. coli O1578:H7 was analyzed and found to be essentially identical to the S. dysenteriae shu locus (Torres and Payne 1997). Some S. boydii and S. flexneri strains also use hemin for growth in low iron, but the genes encoding the transport system do not appear to be closely related to the shu genes.
Analysis of the genomic sequences (Darling et al. 2007; Glasner et al. 2008) in and around the loci encoding the iron transport systems suggests that horizontal transmission of these genes is common (Fig. 2). Only the feo and ent/fep genes appear to share a common ancestry with E. coli K-12. The other Shigella iron transport systems, Iuc, Sit, Iro, Fec and Shu, are all encoded within pathogenicity islands. These appear as insertions, often at tRNA genes, within the E. coli/Shigella genomic backbone and are usually associated with insertion sequences, phage genes, and pseudogenes.
Fig. 2
Fig. 2
Genetics of iron transport systems of Shigella spp.
Aerobactin biosynthesis and transport genes, iucABCD iutA
The aerobactin genes are found within pathogenicity islands in both S. flexneri and S. boydii (Fig. 2). In S. flexneri, the aerobactin operon is encoded within an island located adjacent to the tRNA gene selC (Moss et al. 1999; Vokes et al. 1999) (Fig. 2), a common site of pathogenicity islands in E. coli and Shigella. However, the aerobactin genes are absent from the S. dysenteriae and O157:H7 islands. In S. boydii, the iuc/iutA sequences are highly homologous to the corresponding S. flexneri DNA sequences, but the flanking DNA and the location of the island are different (Purdy and Payne 2001) (Fig. 2). In S. boydii the island is also linked to a phage integrase gene, but the island maps at the pheU locus, rather than selC. In other S. boydii strains, the island is at yet another location (Purdy and Payne 2001).
Shu heme transport locus
The S. dysenteriae shu locus is located between two ORFs that are contiguous in the E. coli K12 genome (Fig. 2) (Wyckoff et al. 1998). There are no insertion sequences or phage-like genes associated with shu, nor is it associated with a tRNA gene. In E. coli O157:H7, the location of the shu locus is the same as in S. dysenteriae, and the DNA sequence of the locus is almost identical (Torres and Payne 1997). Thus, the heme transport genes may have been acquired by a mechanism other than phage or plasmid transfer, or the genes may have been acquired in the distant past, and any associated insertion elements or phage genes have subsequently been lost.
Ferric dicitrate (fec) genes
In one strain of S. flexneri 2a, the fec genes are located within a large island that encodes several antibiotic resistance determinants (Luck et al. 2001). This island also includes a phage-like integrase and is located at the serX tRNA gene. In S. sonnei, the fec genes are flanked by insertion sequences and phage-like genes, but do not appear to be part of a larger island associated with antibiotic resistance elements as in S. flexneri (Darling et al. 2007; Glasner et al. 2008; Yang et al. 2005). The overall organization and location of the S. dysenteriae fec genes is the same as in S. sonnei, but the presence of a stop codon in fecR and a frameshift mutation in fecB indicates that the system is unlikely to be functional in S. dysenteriae.
Salmochelin synthesis and transport, iro genes
The iro locus, which encodes an enterobactin modification system to produce salmochelin as well as transport proteins for this siderophore, is found in S. dysenteriae and appears to be part of an island (Reeves 2001) (Fig. 2). These genes are not found in the other Shigella species.
sitA,B,C,F ferrous iron transport system
The Sit system, which permits transport of both iron and manganese, is found in all Shigella strains that have been tested and in most enteroinvasive E. coli, which also cause dysentery (Runyen-Janecky et al. 2003). In S. flexneri 2a, the genes are flanked by multiple copies of insertion sequences and phage-like genes, including an integrase (Fig. 2). There is considerable diversity in the Sit islands, and even in closely related S. flexneri 2a strains (2457T and 301) the islands are at different locations and there are differences in sequences flanking the sit operon.
Our initial studies to identify iron transport systems involved in virulence were done using S. flexneri 2a strains SA100 and 2457T. These strains have a relatively small number of iron transport systems, making it possible to carry out mutational analysis of each system alone or in combination with one or more other iron transporters. These strains synthesize aerobactin for ferric iron acquisition and use the Feo and Sit transporters for ferrous iron uptake. Mutants lacking one, two or all three systems were constructed and tested for growth in vitro and in cultured epithelial cells (Runyen-Janecky et al. 2003). Mutants with defects in a single iron transport system grew under typical, iron-sufficient laboratory conditions, indicating some overlap in the ability of these systems to provide iron in vitro. The aerobactin synthesis mutant showed a significant growth defect in low iron media, but the sit and feo single mutants did not, suggesting that iron is predominantly in the ferric form under these conditions. The double mutants also grew on iron sufficient medium, although the iuc, feo mutant, which only expresses the Sit system, produced much smaller colonies. The triple mutant (feo, sit, iuc) failed to grow in vitro, even when high levels of iron were added to the medium, showing that no other iron transport systems are present in this strain. The mutant did, however, grow in the presence of exogenously added aerobactin, which it can transport but not synthesize.
The iron transport mutants were also tested in virulence and intracellular growth assays (Table 2). In plaque assays, in which virulent Shigella invade an epithelial cell monolayer and spread intercellularly, giving rise to clear plaques within the monolayer, neither the iuc mutant, nor any of the other single iron transport mutants were defective (Nassif et al. 1987; Runyen-Janecky et al. 2003). However, the aerobactin mutants (iuc) were attenuated for infection of embryonated chicken eggs (Lawlor et al. 1987) or rabbit ligated ileal loops (Nassif et al. 1987). In both cases, the iuc mutant showed less growth in the extracellular spaces of the host. These results indicate that aerobactin may play a role in growth in the extracellular environment, but neither the siderophore nor either of the ferrous iron transporters is essential for intracellular growth and direct cell-to-cell spread. The triple mutant failed to form plaques within HeLa cells, indicating that at least one iron transport systems is required for intracellular growth and that no other, undetected, iron transport systems are functioning in vivo in this strain (Runyen-Janecky et al. 2003). A mutation in fur, encoding a transcriptional regulator of iron homeostasis, resulted in fewer plaques (Boulette and Payne 2007). This strain has reduced invasion of the monolayers due to de-repression of all the iron transport systems and of ryhB, a small RNA that regulates virulence gene expression (Murphy and Payne 2007).
Table 2
Table 2
Effect of mutations in iron transport genes on aerobic and anaerobic plaque formation by S. flexneri a
To better understand the roles of the Shigella iron transport systems in the host cell cytoplasm, we looked at the expression of the iron transport genes in Shigella growing intracellularly (Fig. 1). In a screen of promoter fusions to green fluorescent protein, the sit and fhuA (ferrichrome receptor) promoters were more highly expressed intracellularly than extracellularly (Runyen-Janecky and Payne 2002). Intracellular induction of the feo and iuc operons was not detected in this screen. These results were confirmed by Lucchini et al. (Lucchini et al. 2005) using microarray transcriptional profiling. Additional analysis of the iuc promoter and levels of the IutA outer membrane receptor for aerobactin showed that the aerobactin genes were expressed at a lower level in the intracellular environment compared to extracellular bacteria (Headley et al. 1997). All the Shigella iron transport systems are repressed by iron via Fur. Their promoters contain the consensus Fur binding site, or Fur, and in the presence of iron, Fur binds to the Fur box and represses transcription. Induction of some transporters in the intracellular environment while others are repressed suggests that environmental factors in addition to iron are involved in regulation of iron transport gene expression.
In E. coli, feo is also regulated by FNR, which activates expression of genes in anaerobic environments (Kammler et al. 1993). Because the Feo system transports ferrous iron, its increased expression in low oxygen environments would enhance iron acquisition under conditions where the ferrous form would be expected to predominate. We tested the effects of oxygen on expression of the S. flexneri iron transport systems and on invasion and plaque formation (Boulette and Payne 2007) (Table 3). Using both transcriptional profiling and promoter fusions to gfp, we observed increased expression of the S. flexneri feo genes under anaerobic conditions. In contrast, the sit and iuc genes were induced aerobically. Aerobic induction of sit was unexpected since Sit appears to be a ferrous iron transporter, and under aerobic conditions at neutral pH, the iron will be predominantly in the ferric form.
Table 3
Table 3
Effect of oxygen and the oxygen-dependent regulators ArcA and FNR on expression of S. flexneri iron transport genes
Because the colonic epithelium where Shigella infection occurs is an anaerobic environment, we modified the plaque assay to compare the effects of aerobiosis VS anaerobiosis on function of the iron transport systems during intracellular growth of the bacteria. Epithelial cell monolayers were infected with the wild type or S. flexneri iron transport mutants and incubated either aerobically or anaerobically (Table 2). The triple mutant (iuc, feo sit) was unable to plaque anaerobically or aerobically while the wild type formed plaques under both conditions (Boulette and Payne 2007). The iuc, sit double mutant, which has only the Feo system, formed plaques when the cultured cells were incubated anaerobically, but not aerobically, indicating that the Feo system is functional only under anaerobic conditions. Conversely, the double mutants expressing either the Sit or Iuc systems formed wild-type plaques under aerobic, but not anaerobic, conditions. Thus the plaque formation results were consistent with the in vitro gene expression data; the feo genes, which are induced anaerobically, supported intracellular growth and plaque formation under anaerobic conditions, while the sit and iuc genes, which were induced aerobically, permitted the bacteria to form plaques under aerobic conditions (Tables 2 and and33).
There are two transcriptional regulators, ArcA and FNR, that have been shown to regulate gene expression in response to oxygen in E. coli. Their roles in regulating the S. flexneri iron transport systems was determined by constructing mutants defective in one or both of these environmental response regulators and measuring expression of the iron transport genes in the wild type and mutant backgrounds (Boulette 2007; Boulette and Payne 2007). feo and fhuA expression were reduced under anaerobic conditions in the fnr and arcA single mutants and were further reduced in the double mutant. This indicates that both FNR and ArcA induce expression of feo and fhuA under anaerobic conditions, and the effects of these transcription factors are additive. Analysis of the feo and fhu promoters revealed the presence of potential binding sites for both FNR and ArcA. Although FNR has been shown to regulate feo expression in E. coli (Kammler et al. 1993), little is known about ArcA regulation of iron transport genes. Therefore, the putative ArcA binding sites were mutated to determine whether they were required for induction by ArcA.
Altering the putative ArcA binding sites in the feo and fhuA promoters resulted in loss of ArcA induction of expression. The direct binding of ArcA to the S. flexneri feo promoter at the predicted ArcA binding site was confirmed by showing a shift in electrophoretic mobility for the wild type feo promoter but not the promoter with the altered ArcA box (Boulette 2007; Boulette and Payne 2007). The iuc operon was also regulated by ArcA, but in this case, expression of iuc was reduced anaerobically by ArcA, consistent with the aerobic induction of expression of iuc seen in microarrays. As in the case of feo, ArcA directly binds the iuc promoter in gel-shift assays (Boulette 2007).
Although iuc and sit are both induced under aerobic conditions, the mechanisms of induction are different. Sit expression was not induced aerobically in an arcA mutant indicating that ArcA is needed for aerobic induction, rather than acting as a repressor under anaerobic conditions. The effect of ArcA may be indirect since no binding of ArcA to the sit promoter could be detected (Boulette 2007).
Oxygen not only affects the expression of the individual iron transport systems, it also regulates iron uptake through regulation of Fur, which in turn affects the levels of transcription of each system. fur expression was elevated anaerobically in an arcA mutant, but not in the wild type. Two putative, overlapping ArcA boxes were identified in the fur promoter, and mutating these resulted in loss of ArcA repression. The effect of ArcA on fur is direct, and binding of ArcA to the promoter was demonstrated by gel shift (Boulette 2007).
It is likely that additional levels of regulation exist for controlling expression of the iron transport systems in Shigella. These bacteria must be able to adapt to changes in their environment as they move from cell to cell and from the intracellular to the extracellular environment. Not only will the concentration of iron change, but the ratio of ferric to ferrous iron and of free to complexed iron will vary. The bacteria must obtain sufficient iron to survive and grow with optimal efficiency but must also avoid importing too much iron and risking iron toxicity. Linking expression of the iron transport systems to the level of iron, as well as to environmental conditions such as oxygen that will control the form of available iron, allows the bacteria to express the appropriate iron transport system in a given situation.
Acknowledgments
This work was supported by grant AI16935 from the National Institute of Allergy and Infectious Diseases.
We thank Alexandra Mey for helpful discussions and critical reading of the manuscript.
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