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Logo of biolprocBioMed CentralBiomed Central Web Sitesearchsubmit a manuscriptregisterthis articleBiological Procedures OnlineJournal Front Page
 
Biol Proced Online. 2012; 14: 1.
Published online 2012 January 30. doi:  10.1186/1480-9222-14-1
PMCID: PMC3293049

A method for non-invasive genotyping of APCmin/+ mice using fecal samples

Abstract

The APCmin/+ mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non-invasive method of genotyping may be applied to other transgenic mouse models.

Keywords: APCmin/+, feces, genotyping, cancer, non-invasive

Articles from Biological Procedures Online are provided here courtesy of BioMed Central