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F1000 Biol Rep. 2012; 4: 6.
Published online 2012 March 1. doi:  10.3410/B4-6
PMCID: PMC3292286

Cellular networks controlling Th2 polarization in allergy and immunity

Abstract

In contrast to the development of Th1 (type 1 T helper cells), Th17 and Treg (regulatory T cells), little is known of the mechanisms governing Th2 development, which is important for immunity to helminths and for us to understand the pathogenesis of allergy. A picture is emerging in which mucosal epithelial cells instruct dendritic cells to promote Th2 responses in the absence of IL-12 (interleukin 12) production and provide instruction through thymic stromal lymphopoieitin (TSLP) or granulocyte-macrophage colony stimulating factor (GM-CSF). At the same time, allergens, helminths and chemical adjuvants elicit the response of innate immune cells like basophils, which provide more polarizing cytokines and IL-4 and reinforce Th2 immunity. This unique communication between cells will only be fully appreciated if we study Th2 immunity in vivo and in a tissue-specific context, and can only be fully understood if we compare several models of Th2 immune response induction.

Introduction: Th2 lymphocytes, the lesser gods of adaptive immunity

Th2 cell immunity is something of a two-edged sword. These cells evolved to fight off parasites, but they are also responsible for allergic diseases. Recent advances in understanding Th2 immunity bring us closer to more effective treatments for allergic diseases like allergic asthma and rhinitis, atopic dermatitis and food allergy. These are clearly on the rise in western societies, and pose a significant burden on the health of millions of patients and on health expenditure.

The immune system evolved to neutralize or kill invading pathogens, while at the same time avoiding reactivity to self, harmless commensal organisms and environmental antigens like allergens. Most often, pathogens are neutralized through the effector mechanisms of innate immunity, such as the activation of complement, and phagocytosis and/or killing by macrophages, neutrophils or eosinophils. These innate responses are reinforced by adaptive immunity, in that humoral immunity facilitates complement activation and phagocytosis by innate immune cells and that particular subsets of T lymphocytes help innate effector cells through release of cytokines. CD4+ T helper lymphocytes are divided into broad categories based on the cytokines produced. Th1 lymphocytes produce interferon (IFN)-γ and stimulate the phagocytosis and killing of intracellular bacteria by macrophages. Th17 lymphocytes produce IL-17, which stimulates neutrophils to kill extracellular bacteria and fungi. Th2 lymphocytes produce IL-4, IL-5 and IL-13. IL-5 stimulates the differentiation of eosinophils, which have important roles in killing helminths and other parasites, whereas IL-4 and IL-13 stimulate contraction of smooth muscle and overproduction of mucus, which helps in expulsion of helminths from the gut and lung. The IL-4 (and, to a lesser extent IL-13) produced by Th2 cells also drives the class switching of B cell immunoglobulin production towards immunoglobulin E. Antigen-specific IgE subsequently arms effector cells,, like basophils and mast cells, that express the high affinity IgE receptor (FcepsilonRI, Fc epsilon receptor I), which rapidly degranulate upon re-encounter with the antigen and help in parasite expulsion or resistance to reinfection [1]. Not surprisingly, therefore, Th2 immune responses are often accompanied by activated eosinophils, basophils, and mast cells, as well as goblet cell hyperplasia and functional changes to the surrounding tissues. These activation loops of innate and adaptive immunity need to be closely regulated. Naturally occurring and induced Treg dampen overt inflammatory reactions to microorganisms, and also suppress immunity to self, by suppressing the activation of innate immune cells, the antigen presenting capacity of dendritic cells and the effector function of Th1, Th2 and Th17 cells.

Despite the wealth of information and explosion of recent research on how Th1, Th17 and Treg responses are programmed, relatively less is known about the initiation of Th2 responses. Understanding Th2 immunity is important, as it is central to understanding allergic diseases. Like helminth infection, these diseases are characterized by increased production of IgE antibodies (to inhaled or ingested harmless allergens) and eosinophilic infiltration of the affected tissues. One possible contributing factor to the increase in allergies in the west is that the most commonly used adjuvant for vaccines in humans aluminum hydroxide is also a known Th2 inducer in mice and humans, so understanding its mechanism of action might have great implications for design of better adjuvants [1]. We will not describe the precise molecular mechanisms of Th2 lineage decisions during Th polarization and development, as this is the subject of several recent excellent review articles [2-4]. It has been shown that dendritic cells are at the very heart of inducing T cell responses; however, there has been a lot of debate about how, and even if, they are involved in Th2 response induction. Here, we will discuss the role of dendritic cells in different Th2 models and focus on the communication of dendritic cells with their neighboring epithelial cells and the cells of the innate immune response like basophils, mast cells and eosinophils.

The role of dendritic cells in different Th2 models

Dendritic cells perform a unique sentinel function in the immune response in that they recognize antigens through expression of ancient pathogen pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs), NOD-like receptors, and C-type lectin receptors. These receptors will recognize motifs on virtually any pathogen, allergen, or substance. In addition, dendritic cells have been shown to be sensitive to more generic stress responses, such as oxidative stress, increased rates of protein synthesis and hypoxia, that could also signal the presence of xenobiotics, local tissue injury and disturbance of homeostasis [5,6]. Dendritic cells take up, process and present antigens on their surface, and posses the ability to migrate from the tissues to the draining lymph node. Having all these capabilities, dendritic cells are at the crossroads between innate and adaptive immunity [7].

The study of the functional importance of dendritic cells in various Th2 diseases has been greatly aided by the availability of transgenic models in which dendritic cells can be conditionally depleted or rendered functionally incapable of antigen presentation [9-12]. In one such model, in which cd11cDTR mice were subjected to house dust mite exposure, it was shown that Th2 immunity, as measured by the early production of IL-4 in CD4+ T lymphocytes and the development of airway eosinophilia and IL-4, IL-5 and IL-13 production by mediastinal lymph node T cells, failed to develop [8]. Also, it was shown that Th2 immunity and features of Th2-dependent asthma did not develop following inhalation of the harmless protein antigen ovalbumin in the lung when dendritic cells were eliminated [9,10]. This dependence of Th2 immunity on dendritic cells was also seen when mice with chronic airway remodeling (typically seen in asthma of long duration) were exposed to a new allergen [16]. When dendritic cells were depleted during infection with the parasitic helminth Schistosoma mansoni, Th2 immunity failed to develop and responses skewed towards a Th1 dominated response [11]. In a more chronic model of the intestinal parasite Heligmosomoides polygyrus, Th2 effector responses in the gut were severely affected by dendritic cell depletion [12]. Finally, depletion of CD11chi dendritic cells in mice exposed to ovalbumin antigen in the Th2 adjuvant alum completely abolished Th2 immunity, as measured by the induction of IgE responses and eosinophilic airway inflammation upon ovalbumin aerosol re-exposure in immunized mice [13]. It was shown that a large part of the adjuvant effects of alum were mediated by the in vivo release of the endogenous danger signal uric acid. When uric acid crystals are mixed with harmless ovalbumin and injected intraperitoneally, this also induced Th2 immunity, and this response was abolished in cd11cDTR mice depleted of dendritic cells [14]. These experiments showed that CD11chi dendritic cells are necessary for Th2 immunity. However, it has to be noted that by using the cd11c promoter to deplete dendritic cells, there are also some off target effects. It has been shown that alveolar macrophages and a proportion CD8+ T cells and plasma cells also express CD11c and will, thereby, also be depleted by this treatment [10, 15]. However, adoptive transfer of these affected cell types showed that the effect seen by depleting cells expressing CD11c was due to the depletion of dendritic cells [10].

Having established that dendritic cells are necessary for induction of Th2 immunity, we asked whether they are sufficient. When we transferred as few as 30,000 house dust mite-pulsed lung-derived dendritic cells into the lungs of naïve mice, a Th2 response to house dust mite was induced and eosinophilic airway inflammation developed upon re-exposure of the mice to house dust mite [8]. More precise studies are warranted to define with certainty whether specific subsets of dendritic cells (inflammatory dendritic cells versus subtypes of CD24+CD8α+CD103+ or CD24-CD11b+ conventional dendritic cells) are differentially endowed with potential to induce Th2 immunity. However, this question can be partially, yet indirectly, answered by the fact that Th2 sensitization can be induced by adoptive transfer of GM-CSF cultured bone marrow dendritic cells, most closely resembling mature monocyte-derived CD11b+ dendritic cells, but not by Flt3L cultured bone marrow-derived dendritic cells that more resemble the immature, steady-state dendritic cells resident in the lymph nodes and spleen [14,16,18,19]. Lung dendritic cells can be divided into several subsets [17], grossly divided into CD11c+ conventional dendritic cells and CD11clow plasmacytoid dendritic cells. Conventional dendritic cells can be further divided based on expression of either the myeloid marker CD11b or the integrins CD103 (αEβ7) and langerin (CD207) [20]. During inflammation, monocyte-derived inflammatory dendritic cells are also attracted to the site of inflammation [13].

Function can also be site-dependent. In the skin and gut, CD103+ dendritic cells are primarily involved in cross-presentation of self or foreign antigens to CD8+ T cells, the generation of gut-tropic effector T cells and the induction of Treg. In the lung, however, it has recently been shown that CD11b+ dendritic cells were more efficient at inducing Th2 cells producing IL-4 and IL-10, whereas CD103+ dendritic cells induced greater frequencies of CD4+ T cells producing IFN-γ and IL-17A [18]. The role of plasmacytoid dendritic cells in the induction of Th2 immunity is unclear. Several experiments from several groups have shown that plasmacytoid dendritic cells dampen Th2 immunity to inhaled antigens in vivo [19-21]. However, plasmacytoid dendritic cells have been shown to activate memory Th2 cells in vitro [22]. Recently, it was shown that increased PD-L1 and PD-L2 expression in plasmacytoid dendritic cells activated Th2 effector cells [23]. The precise role of plasmacytoid dendritic cells in suppressing Th2 development in the lung is the subject of intense research. The function of plasmacytoid dendritic cells is in balance with that of conventional dendritic cells and determined by the cytokine osteopontin [24], as well as by activation of the complement system [25,28].

Dendritic cell-independent development of Th2 immunity

Recent papers have proposed that basophils, rather than dendritic cells, are the true inducers of Th2 lymphocyte responses by serving as an early source for the Th2 instructive cytokine IL-4 and, at the same time, acting as antigen presenting cells [26,27]. These authors also suggested that for some antigens, like the model allergen papain or for the helminth Trichuris muris, dendritic cells were neither sufficient nor necessary for Th2 induction. These conclusions were based on the use of mice expressing MHCII exclusively from the cd11c promoter, and reconstituted with CD4+ wild type T cells. These mice poorly reconstitute MHCII expression on migratory inflammatory dendritic cells, which might explain the lack of evidence in this model for a role of dendritic cells as sufficient APCs for inducing Th2 immunity. Another caveat to these studies was the use of the MAR1 antibody to the FcepsilonRI. We, and others, have found that inflammatory dendritic cells are also depleted by this antibody [8,28]. Studies employing genetic strategies to deplete basophils using Cre/lox technology (mcpt8Cre mice) have found that Th2 immunity to papain and the helminth Nippostrongylus brasiliensis is unaffected in the absence of basophils, yet severely depleted when dendritic cells are genetically targeted using cd11cDTA mice [29]. Another recent study showed that in vivo basophils did not interact with antigen-specific T cells in the lymph node and that IL-4 producing basophils could only be found in the affected peripheral tissues [30]. We concur with the view of others that the most likely scenario is that basophils cooperate with dendritic cells to promote Th2 immunity, but do not necessarily have to present antigen to perform this function (Figure 1) [8,31].

Figure 1.
A complex cellular network underlies the initiation of type 2 immune responses

Direct dendritic cell – T cell interaction and Th2 instruction

In the draining lymph node, dendritic cells interact with naïve Th cells and induce their differentiation. Whereas activated CD4+ T cells differentiate into the Th1 lineage in response to IL-12 provided by the dendritic cells, similar signals that initiate Th2 differentiation remain poorly characterized. Th2 differentiation is induced through the actions of the cytokine IL-4 in the absence of IL-12 production by dendritic cells. When we retrovirally overexpressed IL-12, GM-CSF cultured dendritic cells were no longer capable of inducing Th2 immunity in the lungs [32]. Others have proposed that production of IL-6 could be decisive in Th2 instruction [33-35], since IL-4KO mice can still mount a Th2 response [36,37].

Differentiation of naïve Th cells into Th2 cells requires the upregulation of the transcription factor GATA3 [38] and requires activation of STAT-5 (through IL-2 or TSLP) and STAT-6 transcription factors [39]. GATA3 and STAT-6 activation can be upregulated either through IL-4R signalling or Jagged-Notch signalling [40,41]. Notch-dependent signals have been shown to directly regulate GATA-3 expression and critically control Th cell fate in vivo [40,42,43]. However, it has also been suggested that Notch signals provide survival and proliferative advantages to committed Th cells rather than direct commitment to the Th cell fate [44]. Nonetheless, recently Jagged1, and not Jagged2, expression on dendritic cells has been shown to be critical for IL-4 induction and the promotion of allergic responses. Where exactly the STAT-5 activating signal comes from is less well understood. Dendritic cells have been shown to produce IL-2 and TSLP, but these could also be produced by T cells in an autocrine manner upon T cell activation [45].

Besides the expression of Jagged and production of cytokines, other surface markers on dendritic cells can influence Th2 differentiation. The expression of OX40L on dendritic cells has also been shown to be required for optimal Th2 priming [46-48]. The expression of OX40L on dendritic cells is dependent upon signalling through CD40 [47,49,50]. Furthermore, CD40 expression itself is important for Th2 induction as CD40-/- dendritic cells were incapable of inducing Th2 responses to helminth antigens [50]. CD40 interacts with CD154 on T cells and this also seems to be necessary for Th2 immunity. When mice deficient in CD154 were infected with S. mansoni helminths, they did not induce Th2 immunity and suffered from severe morbidity and mortality [51]. More confirmation is provided by the observation that S. mansoni egg preparations prime Th2 cells via the functional modulation of dendritic cells [46,52]. Dendritic cells exposed to parasitic helminth-derived antigens, including SEA, are distinguished by their low production of IL-12, which is thought to be a prerequisite for their Th2-inducing capacity [53]. The omega-1 antigen from Schistosoma applied to dendritic cells in vitro stimulates Th2 development independent of IL-4Rα signalling in vivo [54].

Interaction of dendritic cells with epithelial cells leads to polarization of dendritic cells

Stromal cells have been shown to be important in instructing dendritic cell behavior, particularly in mucosal tissues where epithelial cells represent the first line barrier to the outside world, making them candidates for orchestrating immune responses [55-58]. Some supporting evidence for this comes from the finding that certain dendritic cells are located just below the epithelial lining in the lung, making them easily assessable for epithelial cell-derived factors (Figure 2) [17]. Indeed, chemokines like CCL2 and CCL20 that attract immature dendritic cells and their precursors are produced by airway epithelial cells exposed to Th2 stimuli like house dust mite [59-62]. In response to inhaled allergens, airway epithelial cells secrete a variety of cytokines (GM-CSF, TSLP, IL-25, IL-33, and IL-1 family members), which contribute to dendritic cell maturation and drive them into a Th2-activating mode [59, 63].

Figure 2.
Interactions between airway epithelial cells and dendritic cells

One example of this was shown in mice overexpressing GM-CSF, who showed a break of tolerance to the inhaled harmless antigen ovalbumin, and developed a strong Th2 allergic airway inflammation driven by mature dendritic cells [64]. Also, in a mouse model of house dust mite-induced asthma, GM-CSF was shown to be necessary (but not sufficient) for proper Th2 responses [65-68].

Another well-described Th2-inducing cytokine produced by epithelial cells (but also by mast cells and dendritic cells themselves under inflammatory conditions) is TSLP [45,59,69]. Initially, TSLP was found to potently enhance the maturation of CD11c+ dendritic cells, and TSLP-primed dendritic cells were shown to promote Th2 differentiation. TSLP was reported to exert its Th2-promoting properties through a dendritic cell-mediated pathway that involved induction of OX40L [59,70-72]. Mice overexpressing TSLP in the lung epithelium had increased asthma features [73], whereas blockade of the TSLP-R inhibited key features of allergic asthma [74]. Besides the effects on dendritic cells, TSLP also influences the function of mast cells and basophils, cell types involved in Th2 responses, by acting as a growth factor [75,76]. In specific helminth infections, like H. polygyrus, N. brasiliensis and S. mansoni, TSLP plays a role in the clearance of worms induced by Th2 immunity [77]. While, TSLP is not necessary for clearing all helminth infections, for Trichuris muris it was shown to be indispensible; this could be because T. muris is less efficient in Th2 induction and more potent than other helminths in triggering Th1 responses [3].

IL-25 (also known as IL-17E) was initially reported as a Th2 cell-derived cytokine, but it can also be produced by basophils [72] and airway epithelial cells in response to allergens and respiratory viruses [78,79]. In mice, overexpression of IL-25 in lung epithelial cells led to allergic inflammation, likely due to the capacity of IL-25 to activate dendritic cells to induce Th2 responses [80]. Injection of neutralizing anti-IL-25 antibodies was able to strongly reduce allergic airway inflammation and airway remodelling [78].

The last group of Th2 instructive cytokines discussed here are the IL-1 family members. Some IL-1 family members are produced as pro-cytokines that require cleavage by caspase-1 through the inflammasome activation to be released [81]. IL-1α and IL-1β both bind to the IL-1 receptor 1 (IL-1RI), which is present on almost all cell types, including structural cells such as airway epithelial cells [82,83]. IL-1β is released from cultured airway epithelial cells following protease allergen exposure and enhances the release of the dendritic cell-attracting chemokine CCL20, and of the dendritic cell maturation cytokines TSLP and GM-CSF [84,85]. IL-1R signalling involves the adaptor molecule MyD88, also found downstream of several TLRs. Interestingly, house dust mite-induced Th2 responses in the lung were found to be strongly reduced in MyD88-/- mice [86]; however, pathology induced by S. mansoni infection was unaltered [87]. Also, dendritic cells have been shown to express ST2, the receptor for the IL-1 family member IL-33. This cytokine is expressed in airway epithelial cells where it is constitutively stored in nuclei. Its location is unexpected, and how it is released into the extracellular environment remains unclear. It has been postulated, however, that IL-33 might act as an alarmin in case of allergen exposure [88]. Dendritic cells exposed to IL-33 increased their expression of maturation markers, such as CD40 and OX40L and, thereby, became very potent at inducing Th2 responses [89,90]. Both IL-33 and IL-25 have also been shown to induce an allergic phenotype or helminth clearance independent of Th2 lymphocytes [91,92]. In Rag deficient mice, which lack T and B cells, innate lymphoid cells producing IL-5 and IL-13 are induced by IL-25 or IL-33 [93].

PRR expression by radioresistant stromal cells determines Th2 immune response induction by dendritic cells

Several groups have used radiation chimeric models to study the relative importance of PRRs of the TLR or NLR (NOD-like receptors) family to induction of Th2 immunity. Using chimeric Tlr4-/- animals, we and others have demonstrated that the expression of TLR4 on epithelial cells is crucial in inducing dendritic cell recruitment and intramucosal migratory behaviour in response to LPS and house dust mite [59,94]. In response to house dust mite inhalation, TSLP, GM-CSF, IL-25 and IL-33 are made in an epithelial TLR4-dependent manner [59]. TLR4 is triggered by endotoxin found inside the house dust mite extract and this is facilitated by the house dust mite allergen Der p 2, which behaves like the MD2 chaperone and is important for TLR4 activation [95]. This seems to be a common theme, as many natural and synthetic allergens like cat allergen Fel d 1 and nickel also trigger TLR4. Although some helminths like S. mansoni activate dendritic cells via TLR2 and TLR3, these receptors are dispensable to control infection and pathology [96].

In a recent study, it was shown that Th2 immunity, induced by injection of ovalbumin with agonists of the NLR receptors NOD1 and NOD2, was also dependent on stromal expression of these receptors, acting to induce the systemic production of TSLP and induction of dendritic cell maturation [97]. The precise role of other NLRs like NLRP3 (involved in the formation of the IL-1 and IL-18 inducing inflammasome) in Th2 immunity to natural allergens and alum is very controversial. Initially, it was proposed that Th2 immunity to alum was completely abolished in the absence of Nlrp3 [98], but others have refuted this idea [14,99,100], and it has long been known that alum-induced Th2 asthma models were IL-1R and MyD88-independent [101]. Papers show either an abrogation of alum response in the absence of NLRP3 [98,102], no need for NLRP3 [103,104], or a selective need for NLRP3 [105]. The differences found by the different groups could be due to the type of alum and ovalbumin used and/or mouse strain background.

For natural Th2 immunity to house dust mite in the lung, we and others have found the NLRP3 inflammasome to be irrelevant [14], whereas others have found a lack of the NLRP3 inflammasome to cause a deficiency in Th2 induction when house dust mite was applied to the skin [106]. These studies have certainly aroused interest in inflammasomes, and it remains to be determined how other NLRs are involved in Th2 immune responses to allergens and helminths. Finally, stromal cells (like epithelial cells, and also dendritic cells) express C-type lectin receptors like dectin-2 that have been shown to be involved in Th2 immune responses to house dust mite allergen [107]. Signalling through these receptors often involves the spleen tyrosine kinase pathway that increasingly appears to be crucial for Th2 development [14,34], through, as yet, unidentified mechanisms.

Conclusion

Recent advances are starting to dissect the mechanisms of Th2 development, and a picture is emerging that mucosal epithelial cells instruct dendritic cells to promote Th2 responses by inducing their activation in the absence of IL-12 production. At the same time Th2 stimulates innate immune cells like basophils, mast cells and innate lymphoid cell 2 to produce more polarizing cytokines and ILl-4, which reinforce Th2 immunity. This unique communication network of cells will only be fully appreciated if we study Th2 immunity in vivo in a tissue specific context, and if we compare several models of Th2 immune response induction, like helminth infection, allergen exposure and chemical adjuvants. Given the role of dendritic cells in promoting allergy, greater understanding may yield new targets for prevention and treatment.

Abbreviations

GM-CSF
granulocyte-macrophage colony stimulating factor
IFN
interferon
IL
interleukin
NLR
NOD-like receptors
PRR
pattern recognition receptor
Th
T helper cell
TLR
toll-like receptor
Treg
regulatory T cell
TSLP
thymic stromal lymphopoietin

Notes

The electronic version of this article is the complete one and can be found at: http://f1000.com/reports/b/4/6

Notes

Competing Interests

The authors declare that they have no competing interests.

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24. Xanthou G, Alissafi T, Semitekolou M, Simoes DCM, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V. Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets. Nat. Med. 2007;13:570–8. doi: 10.1038/nm1580. [PubMed] [Cross Ref]
25. Köhl J, Baelder R, Lewkowich IP, Pandey MK, Hawlisch H, Wang L, Best J, Herman NS, Sproles AA, Zwirner J, Whitsett JA, Gerard C, Sfyroera G, Lambris JD, Wills-Karp M. A regulatory role for the C5a anaphylatoxin in type 2 immunity in asthma. J. Clin. Invest. 2006;116:783–96. doi: 10.1172/JCI26582. [PMC free article] [PubMed] [Cross Ref]
26. Perrigoue JG, Saenz SA, Siracusa MC, Allenspach EJ, Taylor BC, Giacomin PR, Nair MG, Du Y, Zaph C, van Rooijen N, Comeau MR, Pearce EJ, Laufer TM, Artis D. MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity. Nat. Immunol. 2009;10:697–705. doi: 10.1038/ni.1740. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 20
Evaluated by Dale Umetsu 01 Jun 2009, Eric Denkers 03 Jun 2009, Richard Grencis 17 Jun 2009, Caetano Reis e Sousa 02 Jul 2009, Thomas Wynn 23 Jul 2009, Jonathan Lamb 09 Sep 2009, Mark Bix 26 Nov 2009
27. Sokol CL, Chu N, Yu S, Nish SA, Laufer TM, Medzhitov R. Basophils function as antigen-presenting cells for an allergen-induced T helper type 2 response. Nat. Immunol. 2009;10:713–20. doi: 10.1038/ni.1738. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 20
Evaluated by Eric Denkers 03 Jun 2009, Shinsuke Taki 04 Jun 2009, Kiyoshi Takeda 12 Jun 2009, Richard Grencis 17 Jun 2009, Caetano Reis e Sousa 02 Jul 2009, Thomas Wynn 23 Jul 2009, Mark Bix 26 Nov 2009
28. Grayson MH, Cheung D, Rohlfing MM, Kitchens R, Spiegel DE, Tucker J, Battaile JT, Alevy Y, Le Yan, Agapov E, Kim EY, Holtzman MJ. Induction of high-affinity IgE receptor on lung dendritic cells during viral infection leads to mucous cell metaplasia. J. Exp. Med. 2007;204:2759–69. doi: 10.1084/jem.20070360. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Marc Rothenberg 03 Dec 2007
29. Ohnmacht C, Schwartz C, Panzer M, Schiedewitz I, Naumann R, Voehringer D. Basophils orchestrate chronic allergic dermatitis and protective immunity against helminths. Immunity. 2010;33:364–74. doi: 10.1016/j.immuni.2010.08.011. [PubMed] [Cross Ref] F1000 Factor 13
Evaluated by Hilde Cheroutre and Daniel Mucida 16 Sep 2010, Richard L Stevens 04 Oct 2010, Rick Maizels and James Hewitson 14 Oct 2010, Troy Randall, Luz Garcia-Hernandez and Javier Rangel-Moreno 10 Feb 2011, Avinash Bhandoola 18 Feb 2011
30. Sullivan BM, Liang H, Bando JK, Wu D, Cheng LE, McKerrow JK, Allen CDC, Locksley RM. Genetic analysis of basophil function in vivo. Nat. Immunol. 2011;12:527–35. doi: 10.1038/ni.2036. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 10
Evaluated by Shinsuke Taki 16 May 2011, Richard Grencis 18 May 2011
31. Tang H, Cao W, Kasturi SP, Ravindran R, Nakaya HI, Kundu K, Murthy N, Kepler TB, Malissen B, Pulendran B. The T helper type 2 response to cysteine proteases requires dendritic cell-basophil cooperation via ROS-mediated signaling. Nat. Immunol. 2010;11:608–17. doi: 10.1038/ni.1883. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 11
Evaluated by Shinsuke Taki 27 May 2010, Juan Rivera and Nicolas Charles 16 Jul 2010, Bart Lambrecht 17 Feb 2012
32. Kuipers H, Heirman C, Hijdra D, Muskens F, Willart M, van Meirvenne S, Thielemans K, Hoogsteden HC, Lambrecht BN. Dendritic cells retrovirally overexpressing IL-12 induce strong Th1 responses to inhaled antigen in the lung but fail to revert established Th2 sensitization. J. Leukoc. Biol. 2004;76:1028–38. doi: 10.1189/jlb.0604325. [PubMed] [Cross Ref]
33. Dodge IL, Carr MW, Cernadas M, Brenner MB. IL-6 production by pulmonary dendritic cells impedes Th1 immune responses. J. Immunol. 2003;170:4457–64. [PubMed]
34. Krishnamoorthy N, Oriss TB, Paglia M, Fei M, Yarlagadda M, Vanhaesebroeck B, Ray A, Ray P. Activation of c-Kit in dendritic cells regulates T helper cell differentiation and allergic asthma. Nat. Med. 2008;14:565–73. doi: 10.1038/nm1766. [PubMed] [Cross Ref]
35. Rincón M, Anguita J, Nakamura T, Fikrig E, Flavell RA. Interleukin (IL)-6 directs the differentiation of IL-4-producing CD4+ T cells. J. Exp. Med. 1997;185:461–9. doi: 10.1084/jem.185.3.461. [PMC free article] [PubMed] [Cross Ref]
36. Brewer JM, Conacher M, Hunter CA, Mohrs M, Brombacher F, Alexander J. Aluminium hydroxide adjuvant initiates strong antigen-specific Th2 responses in the absence of IL-4- or IL-13-mediated signaling. J. Immunol. 1999;163:6448–54. [PubMed]
37. Okahashi N, Yamamoto M, Vancott JL, Chatfield SN, Roberts M, Bluethmann H, Hiroi T, Kiyono H, McGhee JR. Oral immunization of interleukin-4 (IL-4) knockout mice with a recombinant Salmonella strain or cholera toxin reveals that CD4+ Th2 cells producing IL-6 and IL-10 are associated with mucosal immunoglobulin A responses. Infect. Immun. 1996;64:1516–25. [PMC free article] [PubMed]
38. Zheng W, Flavell RA. The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells. Cell. 1997;89:587–96. doi: 10.1016/S0092-8674(00)80240-8. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
39. Zhu J, Guo L, Min B, Watson CJ, Hu-Li J, Young HA, Tsichlis PN, Paul WE. Growth factor independent-1 induced by IL-4 regulates Th2 cell proliferation. Immunity. 2002;16:733–44. doi: 10.1016/S1074-7613(02)00317-5. [PubMed] [Cross Ref] F1000 Factor 10
Evaluated by Dan Conrad 14 Jun 2002
40. Amsen D, Antov A, Jankovic D, Sher A, Radtke F, Souabni A, Busslinger M, McCright B, Gridley T, Flavell RA. Direct regulation of Gata3 expression determines the T helper differentiation potential of Notch. Immunity. 2007;27:89–99. doi: 10.1016/j.immuni.2007.05.021. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
41. Seki N, Miyazaki M, Suzuki W, Hayashi K, Arima K, Myburgh E, Izuhara K, Brombacher F, Kubo M. IL-4-induced GATA-3 expression is a time-restricted instruction switch for Th2 cell differentiation. J. Immunol. 2004;172:6158–66. [PubMed] F1000 Factor 6
Evaluated by Mark Bix 17 May 2004
42. Amsen D, Blander JM, Lee GR, Tanigaki K, Honjo T, Flavell RA. Instruction of distinct CD4 T helper cell fates by different notch ligands on antigen-presenting cells. Cell. 2004;117:515–26. doi: 10.1016/S0092-8674(04)00451-9. [PubMed] [Cross Ref] F1000 Factor 18
Evaluated by David Chaplin 24 May 2004, Chen Dong 03 Jun 2004, Warren Pear 16 Jul 2004, Raphael Kopan 23 Jul 2004, Alan Houghton 27 Jul 2004
43. Fang TC, Yashiro-Ohtani Y, Del Bianco C, Knoblock DM, Blacklow SC, Pear WS. Notch directly regulates Gata3 expression during T helper 2 cell differentiation. Immunity. 2007;27:100–10. doi: 10.1016/j.immuni.2007.04.018. [PMC free article] [PubMed] [Cross Ref]
44. Ong C, Sedy JR, Murphy KM, Kopan R. Notch and presenilin regulate cellular expansion and cytokine secretion but cannot instruct Th1/Th2 fate acquisition. PLoS ONE. 2008;3:e2823. doi: 10.1371/journal.pone.0002823. [PMC free article] [PubMed] [Cross Ref]
45. Kashyap M, Rochman Y, Spolski R, Samsel L, Leonard WJ. Thymic stromal lymphopoietin is produced by dendritic cells. J. Immunol. 2011;187:1207–11. doi: 10.4049/jimmunol.1100355. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Elizabeth Kelly and Jim Stahl 06 Dec 2011
46. Jong EC de, Vieira PL, Kalinski P, Schuitemaker JHN, Tanaka Y, Wierenga EA, Yazdanbakhsh M, Kapsenberg ML. Microbial compounds selectively induce Th1 cell-promoting or Th2 cell-promoting dendritic cells in vitro with diverse th cell-polarizing signals. J. Immunol. 2002;168:1704–9. [PubMed] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
47. Jenkins SJ, Perona-Wright G, Worsley AGF, Ishii N, MacDonald AS. Dendritic cell expression of OX40 ligand acts as a costimulatory, not polarizing, signal for optimal Th2 priming and memory induction in vivo. J. Immunol. 2007;179:3515–23. [PubMed] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
48. Wang Y, Liu Y. Thymic stromal lymphopoietin, OX40-ligand, and interleukin-25 in allergic responses. Clin. Exp. Allergy. 2009;39:798–806. doi: 10.1111/j.1365-2222.2009.03241.x. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
49. Kashiwakura J, Yokoi H, Saito H, Okayama Y. T cell proliferation by direct cross-talk between OX40 ligand on human mast cells and OX40 on human T cells: comparison of gene expression profiles between human tonsillar and lung-cultured mast cells. J. Immunol. 2004;173:5247–57. [PubMed] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
50. MacDonald AS, Straw AD, Dalton NM, Pearce EJ. Cutting edge: Th2 response induction by dendritic cells: a role for CD40. J. Immunol. 2002;168:537–40. [PubMed] F1000 Factor 8
Evaluated by Thomas Wynn 30 Jan 2002
51. MacDonald AS, Patton EA, La Flamme AC, Araujo MI, Huxtable CR, Bauman B, Pearce EJ. Impaired Th2 development and increased mortality during Schistosoma mansoni infection in the absence of CD40/CD154 interaction. J. Immunol. 2002;168:4643–9. [PubMed] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
52. MacDonald AS, Straw AD, Bauman B, Pearce EJ. CD8- dendritic cell activation status plays an integral role in influencing Th2 response development. J. Immunol. 2001;167:1982–8. [PubMed] F1000 Factor 8
Evaluated by Thomas Wynn 30 Nov 2001
53. Jankovic D, Kullberg MC, Caspar P, Sher A. Parasite-induced Th2 polarization is associated with down-regulated dendritic cell responsiveness to Th1 stimuli and a transient delay in T lymphocyte cycling. J. Immunol. 2004;173:2419–27. [PubMed] F1000 Factor6
Evaluated by Bart Lambrecht 17 Feb 2012
54. Everts B, Perona-Wright G, Smits HH, Hokke CH, van der Ham AJ, Fitzsimmons CM, Doenhoff MJ, van der Bosch J, Mohrs K, Haas H, Mohrs M, Yazdanbakhsh M, Schramm G. Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses. J. Exp. Med. 2009;206:1673–80. doi: 10.1084/jem.20082460. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
55. Chieppa M, Rescigno M, Huang AYC, Germain RN. Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement. J. Exp. Med. 2006;203:2841–52. doi: 10.1084/jem.20061884. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 8
Evaluated by David Alpers 12 Apr 2007
56. Hammad H, Lambrecht BN. Dendritic cells and airway epithelial cells at the interface between innate and adaptive immune responses. Allergy. 2011;66:579–87. doi: 10.1111/j.1398-9995.2010.02528.x. [PubMed] [Cross Ref]
57. Lambrecht BN, Hammad H. The role of dendritic and epithelial cells as master regulators of allergic airway inflammation. Lancet. 2010;376:835–43. doi: 10.1016/S0140-6736(10)61226-3. [PubMed] [Cross Ref]
58. Nolte MA, Leibundgut-Landmann S, Joffre O, Reis E, Sousa C. Dendritic cell quiescence during systemic inflammation driven by LPS stimulation of radioresistant cells in vivo. J. Exp. Med. 2007;204:1487–501. doi: 10.1084/jem.20070325. [PMC free article] [PubMed] [Cross Ref]
59. Hammad H, Chieppa M, Perros F, Willart MA, Germain RN, Lambrecht BN. House dust mite allergen induces asthma via Toll-like receptor 4 triggering of airway structural cells. Nat. Med. 2009;15:410–6. doi: 10.1038/nm.1946. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 10
Evaluated by John Kyriakis 14 Apr 2009, Achsah Keegan 05 Jun 2009
60. Nathan AT, Peterson EA, Chakir J, Wills-Karp M. Innate immune responses of airway epithelium to house dust mite are mediated through beta-glucan-dependent pathways. J. Allergy Clin. Immunol. 2009;123:612–8. doi: 10.1016/j.jaci.2008.12.006. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
61. Pichavant M, Charbonnier A, Taront S, Brichet A, Wallaert B, Pestel J, Tonnel A, Gosset P. Asthmatic bronchial epithelium activated by the proteolytic allergen Der p 1 increases selective dendritic cell recruitment. J. Allergy Clin. Immunol. 2005;115:771–8. doi: 10.1016/j.jaci.2004.11.043. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
62. Wada K, Matsuwaki Y, Moriyama H, Kita H. Cockroach induces inflammatory responses through protease-dependent pathways. Int. Arch. Allergy Immunol. 2011;155(Suppl 1):135–41. doi: 10.1159/000327500. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
63. Eiwegger T, Akdis CA. IL-33 links tissue cells, dendritic cells and Th2 cell development in a mouse model of asthma. Eur. J. Immunol. 2011;41:1535–8. doi: 10.1002/eji.201141668. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
64. Stämpfli MR, Wiley RE, Neigh GS, Gajewska BU, Lei XF, Snider DP, Xing Z, Jordana M. GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice. J. Clin. Invest. 1998;102:1704–14. doi: 10.1172/JCI4160. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
65. Cates EC, Fattouh R, Wattie J, Inman MD, Goncharova S, Coyle AJ, Gutierrez-Ramos J, Jordana M. Intranasal exposure of mice to house dust mite elicits allergic airway inflammation via a GM-CSF-mediated mechanism. J. Immunol. 2004;173:6384–92. [PubMed] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
66. Cates EC, Gajewska BU, Goncharova S, Alvarez D, Fattouh R, Coyle AJ, Gutierrez-Ramos J, Jordana M. Effect of GM-CSF on immune, inflammatory, and clinical responses to ragweed in a novel mouse model of mucosal sensitization. J. Allergy Clin. Immunol. 2003;111:1076–86. doi: 10.1067/mai.2003.1460. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
67. Fattouh R, Pouladi MA, Alvarez D, Johnson JR, Walker TD, Goncharova S, Inman MD, Jordana M. House dust mite facilitates ovalbumin-specific allergic sensitization and airway inflammation. Am. J. Respir. Crit. Care Med. 2005;172:314–21. doi: 10.1164/rccm.200502-198OC. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
68. Yamashita N, Tashimo H, Ishida H, Kaneko F, Nakano J, Kato H, Hirai K, Horiuchi T, Ohta K. Attenuation of airway hyperresponsiveness in a murine asthma model by neutralization of granulocyte-macrophage colony-stimulating factor (GM-CSF) Cell. Immunol. 2002;219:92–7. doi: 10.1016/S0008-8749(02)00565-8. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
69. Moon P, Kim H. Thymic stromal lymphopoietin is expressed and produced by caspase-1/NF-κB pathway in mast cells. Cytokine. 2011;54:239–43. doi: 10.1016/j.cyto.2011.03.007. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
70. Ito T, Wang Y, Duramad O, Hori T, Delespesse GJ, Watanabe N, Qin FX, Yao Z, Cao W, Liu Y. TSLP-activated dendritic cells induce an inflammatory T helper type 2 cell response through OX40 ligand. J. Exp. Med. 2005;202:1213–23. doi: 10.1084/jem.20051135. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 8
Evaluated by Mark Bix 08 Mar 2011
71. Liu Y. Thymic stromal lymphopoietin: master switch for allergic inflammation. J. Exp. Med. 2006;203:269–73. doi: 10.1084/jem.20051745. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
72. Wang Y, Angkasekwinai P, Lu N, Voo KS, Arima K, Hanabuchi S, Hippe A, Corrigan CJ, Dong C, Homey B, Yao Z, Ying S, Huston DP, Liu Y. IL-25 augments type 2 immune responses by enhancing the expansion and functions of TSLP-DC-activated Th2 memory cells. J. Exp. Med. 2007;204:1837–47. doi: 10.1084/jem.20070406. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
73. Wong CK, Hu S, Cheung PFY, Lam CWK. Thymic stromal lymphopoietin induces chemotactic and prosurvival effects in eosinophils: implications in allergic inflammation. Am. J. Respir. Cell Mol. Biol. 2010;43:305–15. doi: 10.1165/rcmb.2009-0168OC. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
74. Zhang F, Huang G, Hu B, Song Y, Shi Y. A soluble thymic stromal lymphopoietin (TSLP) antagonist, TSLPR-immunoglobulin, reduces the severity of allergic disease by regulating pulmonary dendritic cells. Clin. Exp. Immunol. 2011;164:256–64. doi: 10.1111/j.1365-2249.2011.04328.x. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
75. Allakhverdi Z, Comeau MR, Jessup HK, Yoon BP, Brewer A, Chartier S, Paquette N, Ziegler SF, Sarfati M, Delespesse G. Thymic stromal lymphopoietin is released by human epithelial cells in response to microbes, trauma, or inflammation and potently activates mast cells. J. Exp. Med. 2007;204:253–8. doi: 10.1084/jem.20062211. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 8
Evaluated by P'ng Loke 23 Feb 2007, Marc Rothenberg 08 Mar 2007, Dale Umetsu 11 Apr 2007
76. Siracusa MC, Saenz SA, Hill DA, Kim BS, Headley MB, Doering TA, Wherry EJ, Jessup HK, Siegel LA, Kambayashi T, Dudek EC, Kubo M, Cianferoni A, Spergel JM, Ziegler SF, Comeau MR, Artis D. TSLP promotes interleukin-3-independent basophil haematopoiesis and type 2 inflammation. Nature. 2011;477:229–33. doi: 10.1038/nature10329. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 16
Evaluated by Booki Min 31 Aug 2011, Richard Grencis 09 Sep 2011, David Voehringer 18 Nov 2011, Marc A Williams 20 Dec 2011
77. Massacand JC, Stettler RC, Meier R, Humphreys NE, Grencis RK, Marsland BJ, Harris NL. Helminth products bypass the need for TSLP in Th2 immune responses by directly modulating dendritic cell function. Proc. Natl. Acad. Sci. U.S.A. 2009;106:13968–73. doi: 10.1073/pnas.0906367106. [PubMed] [Cross Ref]
78. Angkasekwinai P, Park H, Wang Y, Wang Y, Chang SH, Corry DB, Liu Y, Zhu Z, Dong C. Interleukin 25 promotes the initiation of proallergic type 2 responses. J. Exp. Med. 2007;204:1509–17. doi: 10.1084/jem.20061675. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
79. Corrigan CJ, Wang W, Meng Q, Fang C, Wu H, Reay V, Lv Z, Fan Y, An Y, Wang Y, Liu Y, Lee TH, Ying S. T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma. Proc. Natl. Acad. Sci. U.S.A. 2011;108:1579–84. doi: 10.1073/pnas.1014241108. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
80. Kaiko GE, Phipps S, Angkasekwinai P, Dong C, Foster PS. NK cell deficiency predisposes to viral-induced Th2-type allergic inflammation via epithelial-derived IL-25. J. Immunol. 2010;185:4681–90. doi: 10.4049/jimmunol.1001758. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
81. Dinarello CA. A signal for the caspase-1 inflammasome free of TLR. Immunity. 2007;26:383–5. doi: 10.1016/j.immuni.2007.04.005. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
82. Dinarello CA. Immunological and inflammatory functions of the interleukin-1 family. Annu. Rev. Immunol. 2009;27:519–50. doi: 10.1146/annurev.immunol.021908.132612. [PubMed] [Cross Ref]
83. Dinarello CA. Interleukin-1 in the pathogenesis and treatment of inflammatory diseases. Blood. 2011;117:3720–32. doi: 10.1182/blood-2010-07-273417. [PubMed] [Cross Ref] F1000 Factor 8
Evaluated by Ulf Mueller-Ladner 28 Feb 2011
84. Lee H, Ziegler SF. Inducible expression of the proallergic cytokine thymic stromal lymphopoietin in airway epithelial cells is controlled by NFkappaB. Proc. Natl. Acad. Sci. U.S.A. 2007;104:914–9. doi: 10.1073/pnas.0607305104. [PubMed] [Cross Ref]
85. Rusznak C, Sapsford RJ, Devalia JL, Shah SS, Hewitt EL, Lamont AG, Davies RJ, Lozewicz S. Interaction of cigarette smoke and house dust mite allergens on inflammatory mediator release from primary cultures of human bronchial epithelial cells. Clin. Exp. Allergy. 2001;31:226–38. doi: 10.1046/j.1365-2222.2001.01000.x. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
86. Phipps S, Lam CE, Kaiko GE, Foo SY, Collison A, Mattes J, Barry J, Davidson S, Oreo K, Smith L, Mansell A, Matthaei KI, Foster PS. Toll/IL-1 signaling is critical for house dust mite-specific Th1 and Th2 responses. Am. J. Respir. Crit. Care Med. 2009;179:883–93. doi: 10.1164/rccm.200806-974OC. [PubMed] [Cross Ref] F1000 Factor 7
Evaluated by Patricia Finn and Kenneth Liu 14 Jul 2009, Bart Lambrecht 17 Feb 2012
87. Layland LE, Wagner H, da Costa CUP. Lack of antigen-specific Th1 response alters granuloma formation and composition in Schistosoma mansoni-infected MyD88-/- mice. Eur. J. Immunol. 2005;35:3248–57. doi: 10.1002/eji.200526273. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
88. Oboki K, Ohno T, Kajiwara N, Arae K, Morita H, Ishii A, Nambu A, Abe T, Kiyonari H, Matsumoto K, Sudo K, Okumura K, Saito H, Nakae S. IL-33 is a crucial amplifier of innate rather than acquired immunity. Proc. Natl. Acad. Sci. U.S.A. 2010;107:18581–6. doi: 10.1073/pnas.1003059107. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Richard L Stevens 16 Dec 2010
89. Besnard A, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B. IL-33-activated dendritic cells are critical for allergic airway inflammation. Eur. J. Immunol. 2011;41:1675–86. doi: 10.1002/eji.201041033. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Joerg Koehl and Yves Laumonnier 09 Aug 2011
90. Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, Zurawski G, Moshrefi M, Qin J, Li X, Gorman DM, Bazan JF, Kastelein RA. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity. 2005;23:479–90. doi: 10.1016/j.immuni.2005.09.015. [PubMed] [Cross Ref] F1000 Factor 8
Evaluated by Richard Grencis 04 Jan 2006
91. Moro K, Yamada T, Tanabe M, Takeuchi T, Ikawa T, Kawamoto H, Furusawa J, Ohtani M, Fujii H, Koyasu S. Innate production of T(H)2 cytokines by adipose tissue-associated c-Kit(+)Sca-1(+) lymphoid cells. Nature. 2010;463:540–4. doi: 10.1038/nature08636. [PubMed] [Cross Ref] F1000 Factor 22
Evaluated by Richard Locksley 14 Jan 2010, James Disanto 19 Jan 2010, Flavius Martin and Dhaya Seshasayee 01 Feb 2010, Dale Umetsu 11 Feb 2010, Troy Randall 24 Feb 2010, Steve Ward and John Gordon Foster 25 Feb 2010, Avinash Bhandoola 10 Jan 2011, Bart Lambrecht 17 Feb 2012
92. Fallon PG, Ballantyne SJ, Mangan NE, Barlow JL, Dasvarma A, Hewett DR, McIlgorm A, Jolin HE, McKenzie ANJ. Identification of an interleukin (IL)-25-dependent cell population that provides IL-4, IL-5, and IL-13 at the onset of helminth expulsion. J. Exp. Med. 2006;203:1105–16. doi: 10.1084/jem.20051615. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 10
Evaluated by Thomas Nutman 21 Apr 2006, Thomas Wynn 28 Apr 2006
93. Neill DR, Wong SH, Bellosi A, Flynn RJ, Daly M, Langford TKA, Bucks C, Kane CM, Fallon PG, Pannell R, Jolin HE, McKenzie ANJ. Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity. Nature. 2010;464:1367–70. doi: 10.1038/nature08900. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 18
Evaluated by Torben Lund 30 Mar 2010, Lieping Chen 01 Apr 2010, Rick Maizels and James Hewitson 11 May 2010, Dan Conrad 14 May 2010, Bart Lambrecht 17 Feb 2012
94. Tan AM, Chen H, Pochard P, Eisenbarth SC, Herrick CA, Bottomly HK. TLR4 signaling in stromal cells is critical for the initiation of allergic Th2 responses to inhaled antigen. J. Immunol. 2010;184:3535–44. doi: 10.4049/jimmunol.0900340. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
95. Trompette A, Divanovic S, Visintin A, Blanchard C, Hegde RS, Madan R, Thorne PS, Wills-Karp M, Gioannini TL, Weiss JP, Karp CL. Allergenicity resulting from functional mimicry of a Toll-like receptor complex protein. Nature. 2009;457:585–8. doi: 10.1038/nature07548. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 14
Evaluated by Rene Toes and Leendert Trouw 14 Jan 2009, Philipp M Lepper and Kathy Triantafilou 16 Feb 2009, Torben Sigsgaard and Vivi Schlunssen 29 May 2009, Michael Fessler 28 Jun 2011
96. Vanhoutte F, Breuilh L, Fontaine J, Zouain CS, Mallevaey T, Vasseur V, Capron M, Goriely S, Faveeuw C, Ryffel B, Trottein F. Toll-like receptor (TLR)2 and TLR3 sensing is required for dendritic cell activation, but dispensable to control Schistosoma mansoni infection and pathology. Microbes Infect. 9:1606–13. doi: 10.1016/j.micinf.2007.09.013. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
97. Magalhaes JG, Rubino SJ, Travassos LH, Le Bourhis L, Duan W, Sellge G, Geddes K, Reardon C, Lechmann M, Carneiro LA, Selvanantham T, Fritz JH, Taylor BC, Artis D, Mak TW, Comeau MR, Croft M, Girardin SE, Philpott DJ. Nucleotide oligomerization domain-containing proteins instruct T cell helper type 2 immunity through stromal activation. Proc. Natl. Acad. Sci. U.S.A. 2011;108:14896–901. doi: 10.1073/pnas.1015063108. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
98. Eisenbarth SC, Colegio OR, O'Connor W, Sutterwala FS, Flavell RA. Crucial role for the Nalp3 inflammasome in the immunostimulatory properties of aluminium adjuvants. Nature. 2008;453:1122–6. doi: 10.1038/nature06939. [PubMed] [Cross Ref] F1000 Factor 12
Evaluated by Drusilla Burns 28 May 2008, David Chaplin 14 Jul 2008, Dale Umetsu 28 Jul 2008, Shizuo Akira 09 Sep 2008
99. Kuroda E, Ishii KJ, Uematsu S, Ohata K, Coban C, Akira S, Aritake K, Urade Y, Morimoto Y. Silica crystals and aluminum salts regulate the production of prostaglandin in macrophages via NALP3 inflammasome-independent mechanisms. Immunity. 2011;34:514–26. doi: 10.1016/j.immuni.2011.03.019. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
100. Marrack P, McKee AS, Munks MW. Towards an understanding of the adjuvant action of aluminium. Nat. Rev. Immunol. 2009;9:287–93. doi: 10.1038/nri2510. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
101. Schmitz N, Kurrer M, Kopf M. The IL-1 receptor 1 is critical for Th2 cell type airway immune responses in a mild but not in a more severe asthma model. Eur. J. Immunol. 2003;33:991–1000. doi: 10.1002/eji.200323801. [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
102. Li H, Willingham SB, Ting JP, Re F. Cutting edge: inflammasome activation by alum and alum's adjuvant effect are mediated by NLRP3. J. Immunol. 2008;181:17–21. [PMC free article] [PubMed] F1000 Factor 8
Evaluated by Denise M Monack 23 Jul 2008
103. McKee AS, Munks MW, MacLeod MKL, Fleenor CJ, van Rooijen N, Kappler JW, Marrack P. Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity. J. Immunol. 2009;183:4403–14. doi: 10.4049/jimmunol.0900164. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012
104. Franchi L, Núñez G. The Nlrp3 inflammasome is critical for aluminium hydroxide-mediated IL-1beta secretion but dispensable for adjuvant activity. Eur. J. Immunol. 2008;38:2085–9. doi: 10.1002/eji.200838549. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 7
Evaluated by Moriya Tsuji 03 Sep 2008, Bart Lambrecht 17 Feb 2012
105. Kool M, Pétrilli V, Smedt T de, Rolaz A, Hammad H, van Nimwegen M, Bergen IM, Castillo R, Lambrecht BN, Tschopp J. Cutting edge: alum adjuvant stimulates inflammatory dendritic cells through activation of the NALP3 inflammasome. J. Immunol. 2008;181:3755–9. [PubMed]
106. Dai X, Sayama K, Tohyama M, Shirakata Y, Hanakawa Y, Tokumaru S, Yang L, Hirakawa S, Hashimoto K. Mite allergen is a danger signal for the skin via activation of inflammasome in keratinocytes. J. Allergy Clin. Immunol. 2011;127:806–14. doi: 10.1016/j.jaci.2010.12.006. e1-4. [PubMed] [Cross Ref]
107. Barrett NA, Rahman OM, Fernandez JM, Parsons MW, Xing W, Austen KF, Kanaoka Y. Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes. J. Exp. Med. 2011;208:593–604. doi: 10.1084/jem.20100793. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6
Evaluated by Bart Lambrecht 17 Feb 2012

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