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Biomark Insights. 2012; 7: 1–8.
Published online Feb 1, 2012. doi:  10.4137/BMI.S8703
PMCID: PMC3290108
Overcoming the Effects of Matrix Interference in the Measurement of Urine Protein Analytes
Timothy P. Taylor,1 Michael G. Janech,1,2 Elizabeth H. Slate,3 Evelyn C. Lewis,1 John M. Arthur,1,2 and Jim C Oates2,4
1Medical University of South Carolina, Department of Medicine, Division of Nephrology, 96 Jonathan Lucas St., 829 Clinical Science Building, Charleston, SC 29425
2Ralph H. Johnson VA Medical Center, Research Service, Strom Thurmond Building, 114 Doughty St., Charleston, SC 29403
3Florida State University, Department of Statistics, 214 OSB, 117 N. Woodward Ave., P.O. Box 3064330, Tallahassee, FL, 32306
4Medical University of South Carolina, Department of Medicine, Division of Rheumatology and Immunology, 96 Jonathan Lucas St., 912 Clinical Science Building, Charleston, SC 29425
Corresponding author email: oatesjc/at/
Using multiplex bead assays to measure urine proteins has a great potential for biomarker discovery, but substances in urine (the matrix) can interfere with assay measurements. By comparing the recovery of urine spiked with known quantities of several common analytes, this study demonstrated that the urine matrix variably interfered with the accurate measurement of low abundance proteins. Dilution of the urine permitted a more accurate measure of these proteins, equivalent to the standard dilution technique when the diluted analytes were above the limits of detection of the assay. Therefore, dilution can be used as an effective technique for over-coming urine matrix effects in urine immunoassays. These results may be applicable to other biological fluids in which matrix components interfere with assay performance.
Keywords: biomarkers, body fluids urine, analysis/urine, standard addition, assay validation
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