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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Immunity. Author manuscript; available in PMC 2013 February 24.
Published in final edited form as:
PMCID: PMC3288297
NIHMSID: NIHMS353372

Interleukin-4 production by Follicular Helper T cells requires the conserved Il4 enhancer HS V

SUMMARY

Follicular helper T cells (Tfh cells) are the major producers of interleukin-4 (IL-4) in secondary lymphoid organs where humoral immune responses develop. Il4 regulation in Tfh cells appears distinct from the classical T helper 2 (Th2) cell pathway, but the underlying molecular mechanisms remain largely unknown. We found that HS V (also known as CNS2), a 3’ enhancer in the Il4 locus, is essential for IL-4 production by Tfh cells. Mice lacking HS V display marked defects in Th2 humoral immune responses, as evidenced by abrogated IgE and sharply reduced IgG1 production in vivo. In contrast, effector Th2 cells that are involved in tissue responses were far less dependent on HS V. HS V facilitated removal of repressive chromatin marks during Th2 and Tfh cell differentiation, and increased accessibility of the Il4 promoter. Thus Tfh and Th2 cells utilize distinct but overlapping molecular mechanisms to regulate Il4, a finding with important implications for understanding the molecular basis of Th2 mediated allergic diseases.

INTRODUCTION

T helper 2 (Th2) cell immune responses are orchestrated by T cells and innate immune cells that produce the signature Th2 cytokines interleukin (IL)-4, IL-5 and IL-13 (Voehringer et al., 2004). Th2 responses have an important role in protective immunity against parasitic infections (Else et al., 1994), but when inappropriately exaggerated and misdirected to harmless antigens, cause allergic diseases such as asthma (Kay, 2001a, b; Kim et al., 2010). Finding biological modifiers of the Th2 cytokines has emerged as a rational approach in developing new treatments for asthma (Levine and Wenzel, 2010). A complete mechanistic understanding of the molecular details of Th2 cytokine gene regulation may facilitate the development of novel approaches for therapeutic gene silencing in allergic diseases.

Elegant studies using cytokine gene reporter mice identified T cell subsets and innate immune cells that produce Th2 cytokines during Th2 immune responses in vivo (King and Mohrs, 2009; Neill et al., 2010; Price et al., 2010; Reese et al., 2007; Reinhardt et al., 2009; Saenz et al., 2010; Voehringer et al., 2006; Voehringer et al., 2004; Zaretsky et al., 2009). Follicular helper T cells (Tfh) have emerged as the major class of IL-4 producing T cells in the lymph node, and the IL-4 produced by these cells is critically required for shaping Th2-cell based humoral immunity (King and Mohrs, 2009; Reinhardt et al., 2009; Zaretsky et al., 2009). The trans-acting factors required for IL-4 production by Tfh cells are distinctly different (GATA3- and STAT6-independent) from conventional Th2 cells, and the cis-regulatory requirements remain unknown (Reinhardt et al., 2009).

Gene expression in eukaryotes is tightly regulated by the chromatin structure of the underlying gene locus, which in turn influences the accessibility of trans-acting factors and the core transcriptional machinery to their binding sites in proximal gene promoters as well as distal cis-regulatory DNA elements (Berger, 2007; Li et al., 2007). Under physiological conditions, cell-type-specificity of gene expression is primarily conferred by distal cis-regulatory elements (Heintzman et al., 2009; Visel et al., 2009a; Visel et al., 2009b). A number of such elements were identified in the extended (~200 kb) murine Th2 cytokine locus spanning the Il4, Il5, Il13 genes and the constitutively expressed gene Rad50 (Agarwal and Rao, 1998; Ansel et al., 2006; Wilson et al., 2009) (Figure 1A). Targeted deletion of selected cis-regulatory elements in mice demonstrated their non-redundant functions in regulating Th2 cytokine gene expression (Ansel et al., 2004; Koh et al., 2010; Lee et al., 2003; Loots et al., 2000; Mohrs et al., 2001; Solymar et al., 2002; Tanaka et al., 2011; Tanaka et al., 2006; Yagi et al., 2007).

Figure 1
Cis-regulatory regions in the mouse Th2 locus and chromatin analysis of HS V-deficient T cells

We previously identified two putative distal enhancers located 3’ of the Il4 gene, marked by cell-type specific DNase I hypersensitivity (HS V and HS VA) (Figure 1A and S1A). HS V is not accessible in naïve T cells or differentiated Th1 cells, but becomes constitutively accessible in resting Th2 cells; it overlaps a highly conserved non-coding sequence (CNS2) in the Il4 locus (Ansel et al., 2006). HS VA becomes accessible only upon activation of Th2 cells, and the corresponding sequence binds GATA3, STAT6, and NFAT (Agarwal et al., 2000). Combined deletion of a 3.7 kb region spanning both HS V and HS VA resulted in impaired IL-4 and IL-13 production in both Th2 cells and mast cells (Solymar et al., 2002). Confirming these findings, a similar strain of CNS2-deficient mice (Yagi et al., 2007), which bear a smaller deletion that disrupts HS V but also deletes about half of the sequence corresponding to HS VA (Figure S1A), including NFAT and GATA3 binding sequences (Agarwal et al., 2000), also showed impaired IL-4 production in NK T cells and T-CD4 T cells (Sofi et al., 2011; Yagi et al., 2007). Unfortunately, the functional impairment in cytokine production observed in HS V and VA and CNS2-deficient mice could not be unambiguously attributed to one or the other region, since the integrity of both putative regulatory regions was compromised.

There are compelling reasons to examine the function of the HS V (CNS2) region in isolation. The interesting features of this region include (i) constitutive accessibility in Th2 cells (Agarwal and Rao, 1998); (ii) DNA hypomethylation in naïve T cells (Lee et al., 2002); (iii) maintained DNA hypomethylation during Th2 differentiation, but increased DNA methylation during Th1 differentiation (Lee et al., 2002); (iv) binding of a number of important transcriptional regulators – including STAT6, STAT5, GATA3, Notch, RBP-Jκ, ATP dependent chromatin remodeler BRG-1, chromatin looping factor SATB1 and histone methyl transferase MLL – to the HS V region in a Th2-preferential manner (Cai et al., 2006; Liao et al., 2008; Tanaka et al., 2011; Tanaka et al., 2006; Wei et al., 2010; Wurster and Pazin, 2008; Yamashita et al., 2006).

To address these issues, we generated mice bearing a precise deletion of the HS V (CNS2) region. An unexpected finding in the HS V-deficient (ΔV) mice was the complete abrogation of IgE production despite only mild reduction in Th2 responses in affected tissues. To determine whether this dichotomous response was due to the differential requirement for HS V by the cell types that produce IL-4, we made use of allelic IL-4 reporter mice, which allowed us to track IL-4 producing cells in vivo. We show that Tfh cells critically depend on HS V for IL-4 production. In contrast, effector Th2 cells, basophils and eosinophils were far less dependent on HS V.

RESULTS

Deletion of HS V impairs Il4 transcription

To examine the function of HS V in regulation of Th2 cytokine genes, we generated mice with a specific deletion of HS V that did not disrupt the adjacent enhancer, HS VA (Figures 1A and S1). DNase I hypersensitivity analysis of in vitro-polarized HS V-deficient (ΔV) Th2 cells confirmed selective loss of HS V; importantly, other hypersensitivity sites that mark cis-regulatory elements remained intact, including the activation-inducible site HS VA (Figure 1B). Unlike Th2 cells from mice with the combined HS V/VA deletion, which show diminished transcription of all the linked Th2 cytokine genes (Il4, Il13 and Il5) (Solymar et al., 2002), restimulated ΔV Th2 cells showed a nearly 50% reduction in the expression of Il4 and Il13 mRNA, but no significant change (p>0.05) in Il5 and Il10 (Figure 2A). Compared to wild type (WT) Th2 cells the frequency of restimulated ΔV Th2 cells producing IL-4 (mean ± SEM 55% ± 1.3 vs 32% ± 1.3) and IL-13 protein (mean ± SEM 34% ± 1.2 vs 27% ± 1.5) was also reduced by 40% and 20%, respectively, (Figure 2B). As expected, the cytokine profile of ΔV Th1 cells was similar to that of WT Th1 cells (Figures 2A and 2B).

Figure 2
Cytokine gene expression profile of HS V-deficient CD4+ T cells

As a major product of Th2 cells that is also a potent inducer of Th2 differentiation, IL-4 is the key element of a positive feedback mechanism that polarizes Th2 responses both in vitro and in vivo. To assess the requirement for HS V under conditions where this positive feedback was minimal, we generated heterozygous allelic reporter mice in which one Il4 allele derives from KN2 reporter mice (Mohrs et al., 2005), whereas the second is wild type or bears the HS V deletion (designated KN2-WT and KN2-V respectively; Figure 1C). In the KN2 allele, a CD2 gene cassette replaces the first 2 exons of Il4; thus IL-4 protein is not produced but Il4 transcription is faithfully reported as surface expression of human CD2 (huCD2) (Mohrs et al., 2005). Th2 cultures from both allelic reporter mice contained equal numbers of huCD2+ cells, indicating comparable Th2 polarization; among these huCD2+ IL4-competent cells, however, the frequency of IL-4 production was reduced in KN2-V T cells compared to KN2-WT cells, confirming a direct cis-regulatory role for HS V in the control of Il4 activity in Th2 cells (Figure 2C).

To assess the magnitude of positive feedback through IL-4, we compared huCD2 expression in KN2-WT and KN2-KN2 T cells, which do and do not produce IL-4 respectively. As expected, endogenous IL-4 produced from the functional IL-4 allele in KN2-WT T cells strongly potentiated Th2 polarization when the cells were differentiated under suboptimal Th2 conditions, with limiting amounts of exogenous IL-4 provided in culture (Figure 2D and S2A). Similarly, a 2- to 3-fold lower dose of exogenous IL-4 was necessary to induce huCD2 expression in KN2-WT T cells, which produce their own endogenous IL-4, compared to KN2-V T cells, which lack HS V in the functional IL-4 allele (Figure 2E and S2A). At a low concentration of exogenous IL-4 (11 U/mL), the cytokine profile indicated a very strong dependence for HS V in Th2 polarization (Figures 2F and S2B). These results suggested that the deletion of HS V was likely to have pronounced effects on in vivo responses in which IL-4 feedback is important.

In vivo Th2 responses in HS V-deficient mice

To determine the consequences of HS V deficiency in vivo, we used a mouse model of allergic airway disease. Airway hyperresponsiveness (AHR) was reduced in OVA-challenged ΔV mice compared to WT controls (Figure 3A). Peribronchial and perivascular inflammatory infiltrates and mucus hypersecretion typical of allergic inflammation were preserved in ΔV mice (data not shown), but reduced numbers of eosinophils and lymphocytes were found in the bronchoalveolar lavage fluid (Figure 3B). Overall, therefore, the pathological Th2 response in the lungs was partially diminished in ΔV mice.

Figure 3
In vivo Th2 responses in HS V-deficient mice

Immunoglobulin (Ig) isotype switching to IgE, a hallmark of Th2 humoral immunity, is known to be critically dependent on IL-4 production (Finkelman et al., 1988; Reinhardt et al., 2009). Strikingly, the IgE response was completely abolished in ΔV mice (Figure 3C). Flow cytometric measurement of IgE bound to lung infiltrating basophils, confirmed an almost log-scale reduction in the amount of IgE present in ΔV mice (Figure 3D). Since basophil IgE staining in KN2-V mice was very similar to that seen in IL-4-deficient KN2-KN2 mice (Figure 3D), we conclude that HS V is critically required for IL-4 production by the cells that direct IgE responses in vivo.

To corroborate our findings on the effects of HS V-deficiency in the asthma model in vivo, we utilized a Leishmania infection model of Th2 immunity. The magnitude of Th2 responses in this model inversely correlates with the capacity to clear parasites and resolve tissue inflammation (Mohrs et al., 2001). Thus, control C57BL/6 mice that primarily mount a Th1 response effectively cleared the parasite, whereas BALB/c mice, which mount a sustained Th2 response, showed a significant increase in the size of footpad lesions and parasite burden up to 10 weeks after infection (Figures 3E and 3F). We observed no difference between ΔV and WT BALB/c mice, suggesting that in this experimental system, there was no diminution in Th2 tissue responses in the absence of HS V (Figures 3E and 3F). Nevertheless, the Th2 humoral response was again significantly affected in the ΔV mice as reflected by the complete absence of IgE and a reduction of Leishmania-specific IgG1, which is also partly dependent on IL-4 (Kopf et al., 1993); Leishmania-specific IgG2b production, which is IL-4-independent, was unaffected (Figure 3G).

Together these results support an important role for HS V in Th2 humoral immunity in vivo. However, the dichotomous effects of HS V-deficiency on the different arms of Th2 responses, namely the complete absence of the IL-4-dependent humoral response with at most a partial reduction in tissue Th2 responses, indicated that the cell types driving these responses were differentially affected by HS V-deficiency.

HS V-deficient Tfh cells develop normally but fail to produce IL-4

Previous studies with KN2 mice showed that the vast majority of huCD2+ IL4-competent cells in the lymph nodes are CXCR5+PD-1hi Tfh cells that reside in germinal centers and the follicular mantle zone (King and Mohrs, 2009; Reinhardt et al., 2009; Zaretsky et al., 2009). These Tfh cells, or their precursors generated early in immune responses, are the source of IL-4 and other signals that act on B cells to induce IgE and IgG1 production (King and Mohrs, 2009; Reinhardt et al., 2009; Zaretsky et al., 2009). The KN2 allele does not encode IL-4 protein but marks cells that are competent to make IL-4. Therefore, the allelic reporter mice allowed us to ask whether Tfh cell and other in vivo-generated huCD2+ IL-4-competent cells require the HS V cis-regulatory region for IL-4 production from the other allele.

In the asthma model, we observed a significant induction (3-4%) of huCD2+ T cells in the draining parathymic lymph nodes, but not in the non-draining inguinal nodes (0.1-0.3%), 25 days after OVA immunization (Figure 4A, compare top and second panels). Consistent with previous reports (King and Mohrs, 2009; Reinhardt et al., 2009), equal numbers of huCD2+ IL-4-competent cells and CXCR5+PD-1hi Tfh cells were observed in the parathymic lymph nodes of KN2-WT, KN2-V, and KN2-KN2 mice (Figure 4A, second and third panels; quantified in the bar graphs to the right), indicating that IL-4 (lacking in KN2-KN2 mice), and the enhancer activity of HS V (lacking in KN2-V compared to KN2-WT mice), are both dispensable for the generation of Tfh cells in vivo. CXCR5+PD-1hi Tfh cells were predominantly huCD2+ (Figure 4A, third panel, inset histograms), implying that the majority of Tfh cells transcribed the KN2 reporter allele in vivo. Conversely, essentially all huCD2+ cells in the draining lymph nodes also expressed CXCR5 and PD-1 (Figure 4A, bottom panel), confirming that the vast majority of IL-4-competent T cells in this location are indeed Tfh cells under these experimental conditions.

Figure 4
HS V-deficient Tfh cells fail to produce IL-4

The ability of these huCD2+ cells to produce cytokines from the wild type or HS V-deficient Il4 allele was assessed ex vivo by intracellular staining and flow cytometric analysis of IL-4 production following stimulation with PMA and ionomycin for 4 hours. We prevented surface expression of any new huCD2 by inhibiting protein transport with brefeldin A during the entire time of in vitro stimulation, allowing us to focus on cells that were already expressing huCD2 in vivo. As expected of Tfh cells (Reinhardt et al., 2009), the huCD2+CD4+ cells from KN2-WT mice predominantly made IL-4 and not IL-13; strikingly, however, huCD2+CD4+ cells from KN2-V mice almost completely failed to make any IL-4 even under these supraphysiological stimulation conditions (Figure 4B, top panel). As expected, similar results were observed when we restricted our analysis to CXCR5+PD-1hi Tfh cells (Figure 4B, bottom panel). Thus, in Tfh cells exhibiting normal expression of huCD2 from the KN2 Il4 reporter allele, the HS V-deficient allele is completely unable to support IL-4 production, again indicating a critical cis-acting requirement for HS V for Il4 expression in Tfh cells.

These findings were confirmed in Leishmania infection. When CD4+ T cells taken from the draining popliteal lymph nodes 10 weeks after infection were restimulated in vitro, we observed a significant reduction in the number of IL-4 producing cells in V mice compared to WT BALB/c mice (Figure 4C). IL-4 production was also affected in vivo, as evidenced by the near absence of Il4 mRNA in freshly isolated lymph node cells from V mice (Figure 4D).

We next utilized an acute LCMV (lymphocytic choriomeningitis virus) infection model which allowed us to generate relatively larger numbers of IL-4 producing Tfh cells for undertaking detailed mRNA and chromatin analyses (Yusuf et al., 2010). Consistent with our findings in the OVA model, comparable numbers of CXCR5+PD-1hi Tfh cells and germinal center B cells were observed in the lymph nodes and spleen of both WT and ΔV mice (Figure 4E and 4F). Expression of Bcl6, Blimp1, ICOS, SLAM-associated protein (SAP, encoded by Sh2d1a) and Il21 mRNA in FACS-sorted CXCR5+PD-1hi Tfh cells freshly isolated from lymph nodes and spleen were not significantly different between WT and ΔV mice (Figure 4G), further confirming that the enhancer activity of HS V is dispensable for the generation of Tfh cells in vivo. However, IL-4 production was significantly reduced in vivo, as evidenced by the near absence of Il4 mRNA in the FACS sorted CXCR5+PD-1hi Tfh cells from ΔV mice (Figure 4H). We conclude that ΔV Tfh cells are phenotypically normal but have an isolated defect in Il4 transcription.

Early IL-4 production by lymph node T cells is dependent on HS V

Because IgE is produced at early times after sensitization with OVA, we measured OVA-specific IgE, IgG1, and IgG2b at days 9 and 14 after primary immunization with OVA (Figure 5A). Comparison of KN2-WT and KN2-KN2 mice, which produce and lack IL-4 respectively, emphasized the requirement for IL-4 in IgE and IgG1 responses (Figure 5A, left and middle panels). KN2-V mice resembled KN2-KN2 mice in that they failed to generate an IgE response and produced markedly reduced quantities of OVA-specific IgG1 (Figure 5A, left and middle panels). The IL-4-independent IgG2b response was comparable in the three groups of mice (Figure 5A, right panel).

Figure 5
HS V-deficient lymph node T cells fail to produce IL-4 early in the primary immune response

We also assessed cytokine production by huCD2+CD4+Tfh cells after ex vivo stimulation on days 4 and 7 after intraperitoneal OVA-alum immunization. As before (Figure 4B), huCD2+ IL-4-competent parathymic lymph node T cells from KN2-V mice failed to produce IL-4 (Figure 5B). We obtained similar results when T cells in the lung-draining mediastinal nodes were analyzed after inhaled OVA challenge in the asthma model (Figure 5C). Together, these findings support a key cis-regulatory function for HS V in T cells producing IL-4 very early in the immune response, and suggest that Tfh cells and their early precursors share similar requirements for Il4 regulation.

Th2 tissue responses in the lungs of HS V-deficient mice

Given that tissue inflammation and AHR were less severely affected than humoral immune responses in ΔV mice, we hypothesized that the cell types and cytokines that drive Th2 tissue responses are less dependent than Tfh cells on HS V. To test this hypothesis, we again employed Il4 allelic reporter mice, in this case focusing on CD4+ T cells, basophils and eosinophils as the three major IL-4-producing cell types in allergic lung inflammation (Mohrs et al., 2005; Voehringer et al., 2006).

In contrast to Tfh cells, lung-infiltrating Th2 cells produced IL-13 when restimulated ex vivo (Figure 6A, top left panel). Comparing KN2-KN2, KN2-WT and KN2-V mice, a similar proportion of CD4+ T cells expressed huCD2 from the Il4 KN2 reporter allele, which has an intact HS V (Figure 6A, right top panel). Thus IL-4, which is absent in KN2-KN2 mice, is dispensable for the generation of CD4+ Th2 cells in this experimental system. However, the fraction of T cells able to express IL-4 protein from the other allele upon restimulation was reduced in KN2-V mice compared to KN2-WT mice (Figure 6A, top panels). This effect could not be explained by feedback from reduced IL-4 production in vivo, as it was apparent even when we restricted our analysis to huCD2+ T cells or to cells that produced IL-13 upon restimulation (Figure 6B, top panel). Thus HS V enhances Il4 expression in cis in Th2 cells in vivo, consistent with our in vitro findings.

Figure 6
HS V function in IL-4 producing cells infiltrating the lungs in a murine model of asthma

In contrast to Th2 cells, lung-infiltrating basophils produced IL-4 in an entirely HS V-independent fashion upon in vitro stimulation with PMA and ionomycin (Figure 6A, bottom panels). Moreover, in vitro, all huCD2+ basophils produced IL-4 and IL-13, confirming that HS V is not essential for IL-4 production by basophils (Figure 6B, bottom panel). We were unable to measure IL-4 protein production by eosinophils, but FACS-sorted eosinophils from the lungs of OVA-challenged WT and ΔV mice expressed Il4 mRNA at comparable levels (Figure 6C).

Notably, the proportion of basophils expressing huCD2 in vivo was reduced in KN2-KN2 mice compared with KN2-WT mice (Figure 6A, bottom right panel), indicating that IL-4 is important for basophil expression of Il4 in vivo. A similar reduction in huCD2+ basophils was also observed in KN2-V mice (Figure 6A, bottom right panel), suggesting that the source of IL-4 that affects basophil numbers in vivo is strongly HS V-dependent. We speculate that because Tfh cells in KN2-KN2 and KN2-V mice cannot produce IL-4 (Figure 4B), the consequent drastic decrease in IgE (Figures 3 and and5)5) deprives basophils of their ability to use antigen-specific IgE to respond to OVA challenge in vivo.

In summary, unlike the Tfh cells that direct Th2 humoral responses and are strongly dependent on HS V, the cells and cytokines involved in Th2 tissue responses are less dependent, or only indirectly dependent, on HS V for IL-4 production.

HS V affects chromatin accessibility and NFAT binding to the Il4 promoter

To investigate the mechanism by which HS V-deficiency affected Il4 transcription in T cells, we first tested whether loss of HS V affected early transcription of the Il4 gene by naive T cells. Remarkably, naive T cells lacking the HS V (CNS2) region were completely unable to produce Il4 transcripts following ex-vivo stimulation (Figure 7A). These findings are reminiscent of our observations in Tfh cells and their early precursors (Figures 4B, 4F, 5B and 5C), and suggest that all of these cells share similar cis-regulatory requirements for Il4 transcription. In a similar time course assay, in vitro differentiated Th2 cells showed only a 50% reduction in Il4 transcription (Figure 7B).

Figure 7
Chromatin accessibility and NFAT binding in the Th2 locus of HS V deficient T cells

Il4 transcription by naive T cells is strongly dependent on the NFAT-calcineurin pathway, as judged by sensitivity to the calcineurin inhibitor cyclosporin A (CsA) (Ansel et al., 2004) and by the binding of NFAT proteins to the Il4 promoter (Ansel et al., 2004). Chromatin immunoprecipitation (ChIP) assays performed after brief (45 minutes) stimulation showed that binding of the transcription factor NFAT1, the predominant NFAT family member in naive T cells (Macian et al., 2002), was severely reduced in ΔV naive T cells as well as polarized Th2 cell populations (Figure 7B and S3A, B). Together these results suggest an important role for a distal cis-regulatory region, HS V (CNS2), in facilitating NFAT1 binding to the Il4 promoter upon T cell activation.

The diminished binding of NFAT1 to the Il4 promoter in ΔV T cells prompted us to examine the effect of HS V-deficiency on chromatin structure in the Il4 locus in naive T cells and differentiated Th2 cells (Figures 7C and 7D). Because Il4 transcription was selectively affected in T cells, with minimal or no effect on Il13 and Il5 expression, respectively, we focused on known cis-regulatory elements near the Il4 gene: the Il4 promoter; the 3’ Il4 silencer, HS IV (Ansel et al., 2004); HS VA (Agarwal et al., 2000; Agarwal and Rao, 1998); and HS V (CNS2) itself. We limited our analysis to two histone modifications whose association with gene expression has been thoroughly documented: histone-3 lysine-4 dimethylation (H3K4me2), a “permissive” modification found at enhancers and promoters whose presence correlates with increased chromatin accessibility to trans-factors (Birney et al., 2007); and H3K27me3, a “repressive” mark (Bernstein et al., 2006; Wei et al., 2009).

Of the four regions tested, HS V (CNS2) displayed by far the highest enrichment for H3K4me2 in naive T cells and Tfh cells (Figure 7C, black bars) (Baguet and Bix, 2004). H3K4me2 was retained at HS V during Th2 differentiation and increased at the Il4 promoter and HS VA (Figure 7C black bars). HS V-deficiency did not influence H3K4me2 levels in naive T cells and Tfh cells (Figure 7C, compare black and grey bars). In differentiated ΔV Th2 cells, H3K4me2 levels remained lower than WT at HS VA (Figure 7C).

In naive T cells, the Il4 promoter and cis-regulatory regions were also substantially enriched for H3K27me3 (Figure 7D, top panel). Notably, however, Th2 differentiation led to a striking loss of the “repressive” H3K27me3 modification at all four tested regions of the Il4 gene (Koyanagi et al., 2005) (Figure 7D, compare black bars in top and middle panels), and Th2 cells from ΔV mice incompletely erased the repressive H3K27me3 mark, especially at HS VA (Figure 7D, middle panel, compare black and grey bars). Defective erasure of repressive H3K27me3 marks was also observed in ΔV Tfh cells, especially at the Il4 promoter (Figure 7D, bottom panel and S3C). Together, these data indicate that HS V (CNS2) is an important player in the chromatin remodeling events that normally establish an accessible conformation across the Il4 locus in Th2 cells and Tfh cells.

DISCUSSION

We have performed a detailed analysis of mice bearing a precise deletion of HS V (CNS2). Our results show unambiguously that this conserved cis-regulatory element has an important and non-redundant function in enhancing Il4 transcription. In two in vivo models, HS V deficient mice exhibited cell type-specific defects in Il4 expression that manifested in surprisingly dichotomous effects on Th2 immune responses in vivo – a profound reduction of Th2 humoral immunity with total abrogation of IgE production, in the face of only mildly attenuated or unaffected Th2 tissue inflammatory responses.

We used allelic IL-4 reporter mice to uncover differential requirements for HS V among the cell types that drive these responses. We show that Tfh cells, lymph node T cells that make IL-4 early in the primary immune response, and even naïve T cells stimulated ex vivo are strikingly dependent on HS V for Il4 expression. These findings suggest that similar signals and transcription factors are responsible for Il4 expression in all of these lymph node-resident T cells, and likely explain the total abrogation of IgE production and sharply reduced IgG1 responses observed in HS V-deficient mice.

In contrast, Th2 cells derived in vivo or in vitro were only partially dependent on HS V for Il4 expression. IL-4 signaling induces nuclear translocation of STAT6, which is a direct transactivator of both Il4 and Gata3. GATA3, in turn, binds to its own promoter and several cis-regulatory sites in the Th2 cytokine locus, forming a feed-forward positive feedback loop that drives Th2 cell differentiation and the production of IL-13 and IL-5 (Zhu et al., 2010). Notably, HS V deficiency had minimal or no effect on IL-13 or IL-5 production by Th2 cells in vitro or in vivo. Thus, Th2 cells access an HS V-independent IL-4-driven positive feedback loop to drive powerful inflammatory responses in tissues. HS V continues to function as a local enhancer of Il4 in these cells, but has only modest effects on their ability to marshal inflammatory responses.

Allergic inflammation in peripheral tissues also involves innate immune cells. A previous study detected HS V reporter transgenic activity in mast cells and basophils, but found reduced IL-4 expression only in mast cells from mice lacking HS V and part of HS VA (Yagi et al., 2007). Our findings indicate that HS V affects basophil production of IL-4 in vivo, but likely through an indirect mechanism. Given the importance of IgE receptor signaling in basophil activation, it is quite likely that their reduced IL-4 production in HS V-deficient reflects the lack of allergen-specific IgE, This effect may also contribute to the mild reduction in lung inflammation and AHR in HS V-deficient mice.

Notch intracellular domain and its binding partner RBP-Jκ bind to HS V and influence transcription of Il4 in transgenic reporter assays (Amsen et al., 2004; Fang et al., 2007; Tanaka et al., 2006). Disruption of the Notch signaling pathway in mice leads to impaired humoral responses, as evidenced by sharply reduced IgE and IgG1 production and a significant reduction in IL-4 production by T cells in the draining lymph nodes and spleen, which presumably were Tfh cells (Amsen et al., 2007; Tanaka et al., 2006; Tu et al., 2005). Their similarity to our observations in ΔV mice suggest that Notch may mediate its effects on Il4 expression by Tfh cells more directly through HS V, and implicates the Notch pathway as a critical regulator of Tfh cell function and humoral immunity. Further research is needed to determine the relative contribution of Notch and other trans-acting factors in HS V-dependent Il4 expression in Tfh cells, and how these factors mediate their effects on Il4 locus chromatin structure and gene transcription.

To address the molecular mechanism by which HS V selectively affects Il4 gene transcription, we examined the chromatin structure and remodeling events in the Il4 locus during differentiation of naive cells into Th1, Th2 cells (in vitro) and Tfh cells (in vivo). In naive T cells and Tfh cells, HS V displayed by far the highest enrichment for H3K4me2, suggesting increased chromatin accessibility at this site compared with other cis-regulatory elements in the locus (Baguet and Bix, 2004). HS V is also the only site of DNA demethylation between the Il4 promoter and the distal Kif3a gene in naive T cells, suggesting a high degree of accessibility to trans-acting factors during early stages of T cell differentiation when other cis-elements in the locus may be relatively inaccessible (Lee et al., 2002). This feature, and the ability of the HS V (CNS2) region to enhance Il4 transcription in a GATA3 and STAT6-independent manner, likely make HS V particularly critical for Il4 transcription in naive T cells, Tfh cells as well as in T cells that produce IL-4 early in the in vivo immune response.

H3K27me3, a repressive chromatin modification extensively present in the Th2 locus of naive T cells, is removed during Th2 differentiation but maintained during Th1 differentiation (Figures 7D and S3A) (Koyanagi et al., 2005). In ΔV Th2 cells, erasure of the H3K27me3 mark was incomplete across the Th2 locus, especially at HS VA, where the mark was not erased at all. The failure to erase these marks was particularly pronounced in V Tfh cells, correlating with the stringent requirement for HS V for Il4 transcription in these cells. Further investigation is needed to determine whether HS V recruits histone demethylases to the Il4 locus, and to probe the connection between removal of H3K27me3 marks and Il4 promoter and enhancer accessibility for NFAT and other transcription factors that mediate Il4 transcription.

In summary, our experiments have revealed a critical role for the distal Il4 enhancer HS V in Tfh cell function and consequently, Th2 humoral immunity. Mechanistically, HS V (CNS2) is the only region 3’ of the Il-4 gene that bears the permissive H3K4me2 mark at high levels; as such, this conserved enhancer has an important role in shaping chromatin structure in differentiating T cells, as well as facilitating access of trans-acting factors such as NFAT to the Il4 locus. Our data imply that Tfh cells and Th2 cells utilize distinct but overlapping molecular mechanisms to support Il4 locus activity, and may provide insight for more targeted strategies to block Th2 pathology in allergic diseases.

EXPERIMENTAL PROCEDURES

Mice

Mice were used in accordance with protocols approved by the animal care and use committees of the CBR Institute for Biomedical Research, Harvard Medical School, UCSF and LIAI. ΔV mice were generated using standard gene-targeting techniques (details in Supplemental Information).

T cell differentiation, FACS analysis and quantification of cytokine messenger RNA (mRNA) expression

Purification of CD4+ T cells from spleen and lymph nodes, in vitro induction of TH1/ TH2 differentiation, and, restimulation for flow cytometric analysis of intracellular cytokine staining and messenger RNA expression levels were performed as described previously (Ansel et al., 2004) (details in Supplemental Information). In brief, purified CD4+ T cells were stimulated with hamster anti-mouse CD3 (clone 2C11, 0.25 μg/ml) and hamster anti-mouse CD28 (clone 37.51, 1 μg/ml) on plates coated with goat anti-hamster IgG (MP Biomedicals) for 48–60 h under TH1 (IL-12 and anti–IL-4) and TH2 (IL-4, anti–IFN-γ and anti–IL-12) or nonpolarizing conditions. After 2-3 days, cells were removed from the plates and expanded in media with 20 U/ml of recombinant human IL-2 (National Cancer Institute) and analyzed on day 6. For short term stimulation, 5×106 naive T cells were resuspended in media containing 0.5 μg/ml anti-CD3 and 1 μg/ml anti-CD28 and mixed with 2.5×107 latex beads (5μm diameter; Interfacial Dynamics Corporation) coated with goat anti-hamster IgG. Unstimulated controls were cultured with beads but without anti-CD3 and anti-CD28 and were similar to cells held on ice.

Experimental allergic lung disease model and antibody production

Mice were immunized on days 1, 7 and 14 by intraperitoneal (i.p.) injection of 50 μg OVA (Grade V; Sigma Aldrich) /1mg alum (Thermo Scientific) emulsion, followed by intranasal challenge with saline (control) or 100μg OVA on days 21, 22 and 23 as described (Kuperman et al., 2002). On day 24, measurement of airway resistance and BAL fluid total and differential cell counts were performed as described (Kuperman et al., 2002). Primary immune response was induced by a single i.p injection of 50μg OVA/1mg alum emulsion, and serum samples were obtained at different time-points for measuring OVA-specific IgE, IgG1 and IgG2b antibodies by ELISA. Isolation and flow cytometric analysis of immune cells present in lungs and lymph nodes are described in the Supplemental Information.

Leishmania infection model

Amastigotes were serially passaged in the footpads of BALB/c mice to maintain Leishmania major LV39. Four mice per group were infected in the right hind footpad with 1×106 stationary-phase promastigotes. Lesion size was measured with a dial-gauge micrometer (Mitutoyo) biweekly beginning 1 week after infection. To evaluate footpad swelling, we determined the difference in measurement between the right hind footpad and the uninfected left hind footpad. Parasite burdens were counted by limiting dilution assays in which parasites were extracted from ground footpad tissue collected from individual mice. Serum was obtained 9 weeks after infection and total IgE, Leishmania freeze/thaw antigen-specific IgG1 and IgG2b levels were measured by ELISA. Cytokine mRNA from unstimulated popliteal lymph node cells (1×106) was measured by real-time quantitative PCR.

LCMV infection model

LCMV stocks were prepared and quantified as described (McCausland et al., 2007). All infections were done by i.p. injection of 1–2×105 PFU LCMV Armstrong per mouse. Two weeks after infection, CD4+ T cells were isolated from lymph nodes and spleen using a CD4 positive isolation kit (Dynal). Staining for flow cytometry was performed using fluorophore-conjugated antibodies against B220, CD8, PD-1, CD-44, CD62L and CD4 (Ebioscience). CXCR5 staining was performed as described in the earlier section. CXCR5+PD-1hiCD4+CD44hiCD62LCD8B220cells were sorted using a FACS Aria (Becton Dickinson). 3/4th of the sorted TFH cells were fixed (as described below) for chromatin analysis and 1/4th stored in Trizol for mRNA quantification by real-time PCR. For flow cytometric analysis of germinal center B cells, lymph node and spleen cells were stained with antibodies against CD19, CD4, CD8, PNA, FAS, GL7 and IgD and analyzed on a FACS Canto (Becton Dickinson).

Chromatin immunoprecipitation (ChIP)

The detailed protocol is described in the Supplemental Information.

Statistical analysis

A two-tailed Student's t-test was used for statistical analysis. Differences with a P value of less than 0.05 were considered significant.

Supplementary Material

01

ACKNOWLEDGMENTS

The authors thank L. Du, L. Smith, E. Yanni, Z. Yang, Y. Soo Choi, and J. Yang for expert technical assistance, and M. Kubo and S. Crotty for advice and critical reading of the manuscript. This project was funded by the Burroughs Wellcome Fund (to K.M.A), GSK National Clinician Scientist Fellowship Award and Peel Travel Fellowship Award in United Kingdom (to P.V.), NIH grants AI40127 and AI44432 and an award from the American Asthma Foundation (to A.R). L.J.S. is a National Science Foundation (NSF) Graduate Research Fellow. D.B. is the recipient of a Swiss NSF Postdoctoral Fellowship. P.V., G.S., K.M.A., A.R., conceived the work, designed, performed and analyzed experiments, and wrote the paper; L.S., S.A.W., J.I., I.M.D., D.B., assisted in performing some of the experiments under the supervision of P.V., G.S., K.M.A., A.R., A.S.; X.H. performed some of the experiments using the model of allergic airway disease.

Footnotes

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The authors declare no conflict of interests.

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