|Home | About | Journals | Submit | Contact Us | Français|
Locomotor sensitization is a common and robust behavioral alteration in rodents whereby following exposure to abused drugs such as cocaine, the animal becomes significantly more hyperactive in response to an acute drug challenge. Here, we further analyzed the role of cocaine-induced silent synapses in the nucleus accumbens (NAc) shell and their contribution to the development of locomotor sensitization. Using a combination of viral vector-mediated genetic manipulations, biochemistry and electrophysiology in a locomotor sensitization paradigm with repeated, daily noncontingent cocaine (15 mg/kg) injections, we show that dominant negative cAMP-element binding protein (CREB) prevents cocaine-induced generation of silent synapses of young (30 d) rats, whereas constitutively active CREB is sufficient to increase the number of NR2B-containing NMDA receptors (NMDAR) at synapses and to generate silent synapses. We further show that occupancy of CREB at the NR2B promoter increases and is causally related to the increase in synaptic NR2B levels. Blockade of NR2B-containing NMDARs by administration of the NR2B-selective antagonist Ro256981 directly into the NAc, under conditions that inhibit cocaine-induced silent synapses, prevents the development of cocaine-elicited locomotor sensitization. Our data are consistent with a cellular cascade whereby cocaine-induced activation of CREB promotes CREB-dependent transcription of NR2B and synaptic incorporation of NR2B-containing NMDARs, which generates new silent synapses within the NAc. We propose that cocaine-induced activation of CREB and generation of new silent synapses may serve as key cellular events mediating cocaine-induced locomotor sensitization. These findings provide a novel cellular mechanism that may contribute to cocaine-induced behavioral alterations.
In animal models, a hallmark feature associated with chronic exposure to drugs of abuse is locomotor sensitization (Robinson and Berridge, 2000). It has been hypothesized that the molecular and cellular mechanisms mediating locomotor sensitization also contribute to drug-induced pathophysiological emotional and motivational states characteristic of addiction (Robinson and Berridge, 2000, 2001, 2008). Thus, elucidating the molecular and cellular mechanisms underlying the development of this drug-induced psychomotor response would provide significant insight into the neural bases for drug addiction.
The forebrain region nucleus accumbens (NAc) is critically involved in the development of a variety of addiction-related behavioral alterations (reviewed by (Koob and Volkow, 2010)). A prevailing view had been that induction of locomotor sensitization occurs in the ventral tegmental area (VTA), whereas expression of locomotor sensitization is mediated by the NAc (Wolf, 1998; Kalivas, 2004; Kauer and Malenka, 2007; Thomas et al., 2008). However, several studies involving site-specific brain-lesioning, pharmacology, or genetic manipulations consistently demonstrate that the NAc, especially glutamatergic synaptic transmission within the NAc is essential for both the induction and expression phases of cocaine-induced, locomotor sensitization (Kafetzopoulos, 1986; Hooks et al., 1993; Vanderschuren and Kalivas, 2000; Todtenkopf et al., 2002; Kalivas et al., 2009; Maze et al., 2010; Wolf and Ferrario, 2010).
Exposure to cocaine drives insertion of NR2B-containing NMDARs and generates silent synapses in the NAc, with a time course that correlates with the development of locomotor sensitization (Huang et al., 2009). Silent glutamatergic synapses, which express functional NMDAR-mediated currents in the absence of AMPAR-mediated currents, are thought to possess an increased capacity to undergo strengthening of synaptic transmission (Isaac et al., 1995; Liao et al., 1999; Pickard et al., 2000; Groc et al., 2006). Our previous findings demonstrate that cocaine-induced synaptic incorporation of NR2B-containing receptors generates silent synapses in the NAc shell (Huang et al., 2009). These newly generated silent synapses may prime the neuronal circuitry of the animal to engage new connections and support NAc-based long-lasting cellular and behavioral adaptations.
This study aimed to identify the key molecular processes that mediate cocaine-induced generation of silent synapses and, by targeting the identified key molecular components, to examine a possible causal relation of silent synapses to cocaine-induced locomotor sensitization. The transcription factor cAMP-response element binding protein (CREB) is activated within minutes upon cocaine exposure in vivo (Brami-Cherrier et al., 2005; Mattson et al., 2005; Hyman et al., 2006). Upon activation, CREB promotes gene transcription of NR2B (Rani et al., 2005), and CREB generates silent synapses in hippocampal neurons (Marie et al., 2005). Here we show that CREB is critical to cocaine-induced generation of silent synapses in NAc neurons by activation of NR2B gene transcription. Furthermore, intra-NAc inhibition of NR2B-containing NMDARs, which disables silent synapses, prevents the development, but not the expression, of cocaine-induced locomotor sensitization. These findings suggest a potential role for cocaine-induced silent synapses as a structural correlate for cocaine-induced behavioral sensitization.
Male Sprague-Dawley rats (~30 days old) were used. Before drug administration or molecular manipulation, rats were allowed to acclimate to their home cages for >5 days. Two procedures of repeated cocaine administration used in this study were similar to earlier studies (Zhang et al., 2003; Dong et al., 2006). In procedure 1 (referred to as the 5-day procedure), rats received daily injections of either (−) cocaine HCl (15 mg/kg i.p.) or the same volume of saline for 5 days. In procedure 2 (referred to as the 2.5-day procedure), rats received one injection of cocaine (15 mg/kg) on the day 1 afternoon, and two daily injections (8 hrs apart) of cocaine (15 mg/kg) for the following 2 days. The 2.5-day procedure was only used in experiments involving viral expression, in which the in vivo viral injection was performed on day 1 morning. Cocaine injections for electrophysiological and biochemical experiments were performed in a “novel” cage with the same size as the home cage (15 min), walled with white plastic plate and floored with white-diamond soft bedding. Cocaine-treated rats from both procedures were then used for electrophysiological recordings and biochemical assays 24–48 hrs following the last injection. Electrophysiological recordings were preferentially made from the MSNs located in the ventral-medial subregion of the NAc shell, which could be identified by anatomical landmarks.
All procedures were performed by strictly following the standard procedures approved by the Institutional Animal Care and Use Committee at Washington State University.
Sindbis virus (pSINrep2S726), expressing caCREB, dnCREB upstream of an IRES-GFP sequence were produced similar to published protocols (Marie et al., 2005; Dong et al., 2006; Huang et al., 2007) except that the toxicity was further minimized by using a new sindbis virus-based vector, pSINrep (nsP2S726), and its corresponding optimized helper vector DH-BB (tRNA/TE12) (Kim et al., 2004). Some similar results were obtained with herpes simplex viruses, expressing the same constructs.
To deliver the viruses into the NAc shell, we used stereotaxic injection. Briefly, we first anesthetized the rats with a ketamine/xylazine cocktail and installed a guide cannula into the NAc shell (in mm: A, 1.5; L, 0.6; D, 6.5). One week later when rats recovered from surgery, we injected viruses into the NAc shell through the guide cannulae; Concentrated viral solutions (1 µl/side) were infused into the NAc shell through a pump at a flow rate of 0.2 µl/min. The injection cannula was then slowly withdrawn and the rats were placed on the warmed heating pad for post surgical recovery. Because the whole injection process took <10 min with minimal disturbance, we only briefly anesthetized the rats with isoflurane for this purpose. After waking up, rats were transferred to regular housing cages. We identified the infected neurons in living slices by the GFP signal using epifluorescence microscopy.
The expression efficacy varied between cells and we chose to record cells with only weak GFP-signals with an assumption that relatively lower expression efficacy exerted less non-specific effects. All virally infected cells were recorded on day 3 after the injection day except for the caCREB-expressing cells, for which the recording was performed 24 hrs after injection.
We validated the new virus by recording from uninfected NAc neurons and neurons expressing GFP alone. Neither the break-in resting membrane potential (in mV: uninf, −81.7 ± 0.8, n = 19; GFP, −80.8 ± 0.9, n = 13, p = 0.47) nor the break-in membrane resistance (in MΩ: uninf, 446.6 ± 14, n = 20; GFP, 438.9 ± 15, n = 12) was affected by the expression of pSINrep (nsP2S-GFP). Furthermore, we compared AMPAR/NMDAR ratio, a parameter that reflects the basic property of excitatory synapses, between uninfected NAc neurons and GFP-expressing neurons. Again, no difference was observed (uninf, 1.2 ± 0.1, n = 25; GFP, 1.3 ± 0.1, n = 6, p > 0.1, AMPAR EPSCs were measured at −80 mV and NMDAR EPSCS were measured at +40 mV). When performing virus-related experiments, we always included uninfected and GFP-expressing cells as controls.
To study the involvement of CREB in cocaine induction of dendritic spines, we used herpes simplex virus (HSV) vectors based on published protocols (see (Carlezon et al., 2005)). dnCREB (S133A mutant) was expressed under control of a CMV promoter. GFP was expressed via the same transcript following an IRES sequence, both downstream of dnCREB. The control vector expressed IRES-GFP alone.
Similar to that described before (Dong et al., 2006), the rat was decapitated following isoflurane anesthesia. The brain was removed and glued to a block and sliced with a vibratome in 4°C artificial cerebrospinal fluid (aCSF). Coronal slices of 300–450 µm thickness were then cut such that the preparation contained the signature anatomical landmarks (e.g. the AC) that clearly delineated the NAc subregions. After allowing 1–2 hours for recovery, one slice was transferred from a holding chamber to a submerged recording chamber where it was continuously perfused with oxygenated aCSF maintained at 33°C. It is important to note that temperature plays a key role in measuring the decay kinetics of NMDAR EPSCs. It has been shown that when the recording temperature is increased from 25°C to 33°C, the same recorded neurons exhibited NMDAR EPSCs with much larger amplitude (~1.6 folds) and much faster decay time course (~2.5 folds) (Losi et al., 2002). Thus, the properties of NMDAR EPSCs measured at lower temperatures might be much different from that measured at 33°C.
Standard whole-cell voltage-clamp recordings from NAc shell were done with a MultiClamp 700B amplifier (Molecular Device) in all electrophysiological experiments. The intra- and extracellular solutions used can be found in our published papers (Dong et al., 2006; Huang et al., 2007). The extracellular solution routinely contained picrotoxin (0.1 mM) to block GABAAR-mediated currents and additionally NBQX (5µM) for isolating NMDAR-mediated currents. When measuring regular EPSCs, presynaptic stimuli (intensity, 200–500 µA; duration, 300–600 µs; frequency, 0.1 Hz) were applied through a bipolar microelectrode. The stimulating electrode was placed close to recorded neurons (~3 –4 cells away) and the amplitude of EPSCs was adjusted within ~80–150 pA; both of these efforts were made to minimize the potential spatial effect (Williams and Mitchell, 2008). Amplitudes of AMPAR EPSC were calculated by averaging 25 EPSCs at −80 mV and measuring the peak (2 ms window) compared to the baseline (2 ms window). NMDAR EPSC amplitudes were calculated by averaging 25 EPSCs at +40 mV and measuring the amplitude (2 ms window) 35 ms after the EPSC peak compared to the baseline.
The coefficient of variation (CV) analysis was done as previously described (Kullmann et al., 1996; Huang et al., 2009). Briefly, CVs were estimated for epochs of 50 consecutive trials. Sample variances (SVs) were calculated for EPSC amplitudes and for noise sweeps. The CV was calculated as the square root of the difference for the sample variances [SV(EPSC)-SV(Noise)], divided by the mean. The CV of NMDAR EPSCs was higher than previously reported, because the NMDAR EPSC amplitude measurements were made 60 ms after EPSC onset in the absence of NBQX, not at the peak of the NMDAR EPSC, thus adding variance to the measurement. Minimal stimulation experiments were performed as described previously (Kullmann, 1994; Marie et al., 2005). Briefly, the frequency of presynaptic stimulation was set at 0.33 Hz. After obtaining a small (>40 pA) EPSCs at −80 mV, stimulation intensity was reduced in small increments to the point that failures vs. successes could be clearly distinguished visually. Stimulation intensity and frequency were then kept constant for the rest of the experiment. The amplitude of both AMPAR and NMDAR EPSCs resulting from single vesicle release is relatively large (e.g., ~15 pA for AMPAR mEPSCs), (Huang et al., 2007) which greatly facilitate the judgment of success vs. failure. Failures vs. successes were defined visually. Percent silent synapses were calculated using the equation: 1-Ln(F−80)/Ln(F+40), in which F−80 was the failure rate at −80 mV and F+40 was the failure rate +40 mV.
Decay kinetics of NMDAR EPSCs was assessed using the time decaying from the peak amplitude to ½ peak amplitude of EPSC (Barria and Malinow, 2005). The NMDAR EPSCs used for analysis was obtained by averaging 20–30 consecutive individual EPSCs. Alternative measurements were also applied and similar results were obtained.
D-APV (D-Aminophosphonovaleric acid), referred to as APV in this manuscript, was used to inhibit total NMDARs. Ro256981 at a concentration of 20 nM was used to selectively inhibit NR2B-containing NMDARs. NBQX, APV, and Ro256981 were purchased from Tocris (UK) and other chemicals were purchased from Sigma-Aldrich (St Louis) unless specified.
We adapted a method from our previous study to quantify protein levels in virally manipulated NAc slices (Huang et al., 2007). Briefly, we isolated the NAc shell from 32-day old rats and put the tissues on slice culture films (Millipore) on the neural basal-A culture medium (containing B27 supplements, Invitrogen). One hour later, we dropped the virus solution (containing caCREB-GFP-, dnCREB-GFP-, or GFP-expressing sindbis viruses) on the tissues and continued to culture for 18 hrs. We then collected and coded (to set up a blind analysis) the tissues for western assay. Slices were homogenized and sonicated in lysis buffer containing proteinase and phosphatase inhibitors (20 mM Tris, 50 mM Nacl, 1% Triton X-100, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, PH 7.4) followed by end-over-end mixing for 30 min at 4°C. The homogenates were centrifuged at 14,000 rpm for 15 min at 4°C. The supernatants were processed by SDS-PAGE and subjected to western blotting. Antibodies included NR2B monoclonal antibodies (Upstate, Charlottesville, VA), and β-actin monoclonal antibody (Sigma, St. Louis, MO). Bands were visualized by enhanced chemiluminesence (ECL, Amersham) and band intensities were quantified by NIH image software. The total NMDAR subunit levels were normalized to the actin level in each sample.
Chromatin immunoprecipitation (ChIP) was performed following previously described methods (Kumar et al., 2005). Briefly, animals were treated with cocaine (15 mg/kg) or saline for 5 days. Bilateral 14 gauge NAc punches were pooled from 5 animals (ten punches), where n corresponds to each pool of animals. Punches were briefly fixed in 1% formaldehyde; fixation was stopped using 2 M glycine. 1 day prior to sample sonication, sheep anti-rabbit IgG magnetic beads were prepared by incubating appropriate magnetic beads with anti-phospho-CREB (rabbit polyclonal ChIP grade, Abcam) antibody overnight at 4°C under constant rotation in block solution. For each ChIP sample, five animals (ten punches) were pooled and sonicated on ice with a Fisher Sonic Dismembrator (Model F550) once for 25 s, followed by three 15 s sonications. Samples were centrifuged, and supernatant was removed to a new tube. Each sample was immunoprecipitated with anti-rabbit antibodies directed against phospho-CREB. Eighty microliters of chromatin were removed from each sample to use as input (i.e., nonimmunoprecipitated control). Reverse cross-linking was performed with 5 M NaCl, 0.5 M EDTA, and Tris-HCl, pH 6.5, at 65°C, and proteins were digested with RNA-grade proteinase K (Invitrogen). DNA was purified and resuspended in 200 ul of RNase–DNase-free water (Invitrogen).
For ChIP samples, primers were designed to amplify products of 150 bp in the regions immediately 5’ to the start site of NR2A and NR2B. PCR was performed using an Applied Biosystems 7500 quantitative reverse transcription-PCR system with the following cycle parameters: 2 min at 95°C; 40 cycles of 95°C for 1 min, 60°C for 33 s, 72°C for 33 s; and graded heating to 95°C to generate dissociation curves for confirmation of single PCR products. Data were analyzed by comparing C(t) values of the treatment condition (cocaine) to the control condition (saline) using the 2^-ΔΔC(t) method (Kumar et al., 2005) (Livak and Schmittgen, 2001). Briefly, the input C(t) values for each sample were subtracted from the immunoprecipitated C(t) values (ΔC(t)). These values were averaged for all of the saline samples and this value (average ΔC(t)) was subtracted from the ΔC(t) generated for each sample. This gives the ΔΔC(t) value for each sample. Two was raised to the negative power of each ΔΔC(t) giving the fold change induced by cocaine for NR2A and NR2B.
Dendritic spines of NAc MSNs were labeled and imaged as described before (Shen et al., 2008). Briefly, all rats were perfused transcardially (20 ml/min) with 0.1 M sodium phosphate buffer (PB), followed by 200 ml 1.5% paraformaldehyde (PFA) in 0.1 M PB. As has been previously reported, the use of 1.5% rather than the more traditional 4% PFA was critical in obtaining maximal dye filling of small diameter spines. Brains were removed and postfixed in the same fixative for 1 h at room temperature before coronal slices of 150 um thickness were prepared using a vibratome at room temperature. The slices were collected in PB-saline and mounted prior to DiI labeling. DiI fine crystals (invitrogen, CA) were delivered under a dissecting microscope onto the surface of slices using a borosilicate glass micropipette with a sharp and elongated tip, controlled by a micromanipulator (Sutter, CA). DiI was allowed to diffuse in PBS for 24 hrs at 4°C, and then labeled sections were fixed in 4% PFA at room temperature for 1 h. After brief wash in PBS, tissues were mounted in aqueous medium prolong (Invitrogen).
An Olympus confocal microscope was used to image the labeled sections. DiI was excited using the Helium/Neon 543 nm laser line. The entire profile of each DiI-positive neuron to be quantified was acquired using a 40X oil immersion objective. After the neuron was scanned and confirmed as the NAc shell MSN, its dendrites were focused using a 100X oil immersion objective and scanned at 1 µm intervals along the z-axis for a maximum of 200 planes and the final image of each dendrite was obtained by stacking all planes. Analyses were performed on 2D projection images using the software Image J (NIH). Based on previous results (Robinson and Kolb, 2004), secondary and tertiary dendrites were preferentially sampled. For each neuron, 1–2 dendrites of >20 µm in length were analyzed. For each of the eight rats, at least ten neurons were analyzed. We divided the spines into two large categories: The filopodia-like protrusion was operationally defined as the protrusion without a clear head or with a head but its diameter was less than 2 times the diameter of its neck. The mushroom-like protrusion was operationally defined as the headed protrusion with the diameter of the head >2 times of its neck.
For experiments involving dnCREB, dendritic spines were imaged by GFP expression by confocal microscopy and quantified by observers blind to the experimental conditions. This method has been validated in several previous publications (e.g. Russo et al., 2009; Maze et al., 2010).
Rats were anesthetized with zyket (ketamine 87 mg/kg + xylazine 13 mg/kg) and placed into a stereotaxic apparatus. Bilateral 26 gauge stainless steel guide cannulae (Plastics One, Roanoke, VA) were implanted into the NAc shell (1.0 from bregma, ± 1.0 mm from midline and −6.0 mm from skull) and mini osmotic pumps were implanted. Rats recovered one week prior to experimentation. The perfusion rate of the osmotic pump was set at 0.1 µl/hr. The in vivo perfusion concentrations of Ro256981 and APV were set at 20 and 500 nM, respectively. Rats that received intra-NAc saline perfusion through mini pumps were used as sham controls. Rats were allowed to habituate to the locomotor chamber (16" L × 16" W × 12" H) 1 hour per day for 2 days before the day 0 injection of saline. Rats then received a 15 mg/kg daily i.p. cocaine injection for 5 days (the same volume of saline injection was used as control). Locomotor activity was immediately measured for 1 hr and the data from the first 15 min were presented. In a subset of rats, the spontaneous locomotor activity was measured in the same setup before cocaine treatment. After 2 days of withdrawal from the last cocaine/saline injection, rats received a challenge injection of cocaine (12 mg/kg) and their locomotor activity was tested immediately. At the end of the experiment, all cannulae placements were verified with neutral red staining of coronal brain sections.
In experiments of CV assays, minimal stimulation assays, and western blot assays, all data were obtained blindly. In experiments involving measuring the decay kinetics of NMDAR EPSCs (Fig. 3) and mNR1-containing NMDARs (Fig. 4), ~75% of the data were obtained blindly. All results are shown as mean ± SEM. “n” refers to the number of animals examined. For electrophysiological experiments, two to three cells were recorded from each rat. The total number of cells examined in each set of experiments was also presented (referred to as “m”). For morphological experiments, five to twenty dendrites were examined. The total number of dendrites in each set of experiments was also presented (referred to as “m”). All statistics were performed based on animals. In electrophysiological and morphological experiments, the averaged value of the measured parameters from all examined cells or dendrites was used to represent the value of each rat for statistics. Statistical significance was assessed using t-tests, or one-way or two-way ANOVA with Bonferroni posttests.
To identify the molecular mechanism underlying cocaine-induced generation of silent synapses, we focused on CREB, a transcription factor activated in response to cocaine exposure (reviewed by (Carlezon et al., 2005)) and implicated in synaptogenesis (reviewed by (Lonze and Ginty, 2002)). To test a requirement for CREB in generation of silent synapses, we expressed constitutively active CREB (caCREB) or a dominant negative form of CREB (dnCREB) by means of the sindbis viral expression system by stereotaxic injection directly into the NAc in vivo.
We examined cocaine-generated silent synapses using two independent functional electrophysiological assays, coefficient of variation analysis (CV) and minimal stimulation assay (Huang et al., 2009). The CV is dependent on the number of recruited synapses during trials of evoked EPSCs (Kullmann, 1994). Comparing the CV of the AMPAR component (measured at −80 mV) and the CV of the NMDAR (measured at +40 mV) elicited by the same stimulation strength provides a relative measure of changes in the receptor content of synapses. We compared the variability of evoked NMDAR EPSCs to that of AMPAR EPSCs as the ratio CV(NMDAR)/CV(AMPAR) in different experimental groups. Expression of caCREB for 24 h decreased the CV of NMDAR EPSCs (F5,33 = 4.65, p < 0.01, one-way ANOVA; p < 0.05, caCREB vs. uninf-saline or GFP-saline; Bonferroni posttest; n/m (total animals/total cells) = 6/10 for caCREB, 12/29 for uninf-saline, and 5/9 for GFP-saline) and CV(NMDAR)/CV(AMPAR) (F5,33 = 13.57, p < 0.01, one-way ANOVA; p < 0.01, caCREB vs. uninf-saline or GFP-saline, Bonferroni posttest; Fig. 1A, B) with no significant effect on the CV of AMPAR EPSCs (F5,33 = 1.81, p = 0.14, one-way ANOVA), consistent with an increase in silent synapses. A similar reduction in the CV of NMDAR EPSCs (p < 0.01, uninf-saline vs. uninf-cocaine, Bonferroni posttest; n/m = 12/29 for uninf-saline and 5/9 for uninf-cocaine) and CV(NMDAR)/CV(AMPAR) (p < 0.01, uninf-saline vs. uninf-cocaine, Bonferroni posttest) was observed when rats were treated for 2.5 days with cocaine, a regimen adapted for the time course of the viral manipulations. Importantly, the changes in CV elicited by this shortened cocaine procedure were comparable to those of our previous study with a five-day procedure (Huang et al., 2009).
Whereas expression of dnCREB alone did not affect the CV of NMDAR EPSCs (p = 1.0, dnCREB-saline vs. GFP-saline or uninf-saline, Bonferroni posttest; n/m = 5/9 for dnCREB-saline, 5/9 for GFP-saline, and 12/29 for uninf-saline) and the ratio of CV(NMDAR):CV(AMPAR) (p = 1.0, dnCREB vs. GFP-saline or uninf-saline, Bonferroni posttest; Fig. 1A, B) in saline-treated rats, it prevented the effects of cocaine on the CV(NMDAR)/CV(AMPAR) in the 2.5-day procedure (p = 0.49, dnCREB-cocaine vs. uninf-saline, p < 0.05, dnCREB-cocaine vs. uninf-cocaine, Bonferroni posttest; n/m = 6/9 for dnCREB-cocaine; Fig. 1A, B). Note that expression of GFP alone did not affect either of the above parameters (p = 1.0, GFP vs. uninf-saline for CV-AMPAR, CV-NMDA, and CV(NMDAR)/CV(AMPAR), Bonferroni posttests; Fig. 1A, B). The findings that both GFP and dnCREB did not by itself alter the NMDA or AMPA CV, demonstrate that delivery of viral vectors under our experimental conditions did not themselves affect these parameters. As such, the manipulated groups (virally infected cells) were directly compared with uninfected cells in most comparisons.
In the minimal stimulation assay (see Material and Methods), we adjusted the stimulation strength of the AMPAR EPSCs such that successful trials with a measurable EPSC were interleaved with trials with no detectable EPSC (failures) (Fig. 1C). We calculated the estimated percentage of AMPAR-silent synapses from the failure rates of AMPAR EPSCs and NMDAR EPSCs (see Material and Methods for details) (Kullmann, 1994; Marie et al., 2005; Huang et al., 2009). Expression of caCREB for 24 h induced an increase in the percentage of failures, a predictor of the percentage of silent synapses in cells of saline-treated animals, compared to GFP-expressing control cells (F6, 32 = 12.06, p < 0.01, one-way ANOVA; p < 0.01, caCREB vs. uninf-saline or GFP-saline Bonferroni posttest; n/m = 6/12 for caCREB-saline, 5/8 for uninf-saline, and 5/10 for GFP-saline; Fig. 1C, D). Similarly, cocaine administration in the 2.5-day procedure increased the percentage of failures, consistent with the generation of silent synapses in control and GFP-expressing neurons, confirming our previous results with a five-day procedure (p < 0.05, uninf-saline vs. uninf-cocaine, p < 0.05 GFP-saline vs. GFP-cocaine, Bonferroni posttest; n/m = 5/8 for uninf-cocaine and 5/10 for GFP-cocaine; Fig. 1C, D) (Huang et al., 2009). Whereas dnCREB expression did not significantly alter the percentage of silent synapses in saline treated rats (p = 1.0, dnCREB-saline vs. uninf-saline or GFP-saline; Bonferroni posttest; n/m = 5/12), expression of dnCREB prevented the generation of silent synapses in cocaine treated rats (p < 0.05 dnCREB-cocaine vs. uninf-cocaine, p < 0.01, dnCREB-cocaine vs. GFP-cocaine, p = 1.0 dnCREB-cocaine vs. uninf-saline or GFP-saline, Bonferroni posttest; n/m = 8/16 for dnCREB-cocaine; Fig. 1C, D).
In the above experiments, we used relatively young (~30 d) rats. Young rats exhibit higher basal level of silent synapses (Durand et al., 1996; Hsia et al., 1998; Kerchner and Nicoll, 2008), which may lead to different behavioral responses to drugs of abuse compared with more matured rats (Andersen et al., 2002; Tirelli et al., 2003). However, it has been shown that silent synapses are generated in the NAc of both young and adult rats upon cocaine exposure (Huang et al., 2009), and similar to adult rats (~65 d), young rats used in this study exhibited robust cocaine-induced locomotor sensitization (see below). These findings suggest that activation of CREB in NAc neurons is both necessary and sufficient for cocaine-induced generation of silent synapses.
Neurons in the NAc are highly heterogeneous and can be divided into different subpopulations depending on different criteria. For example, based on receptor/transmitter subtypes, the NAc neurons can be divided into two subpopulations, one (~50% of total NAc neurons) co-expressing dopamine D1-class receptors and substance P, and the other (~50% of total NAc neurons) co-expressing dopamine D2-class receptors and enkephalin (Le Moine and Bloch, 1995; Gong et al., 2003; Lee et al., 2006a). This distribution includes a heterogenous population of 5–10% interneurons. Based on electrophysiological properties, NAc neurons can be categorized as neurons with (~24%) or without gap junctions with their neighboring NAc neurons (O'Donnell and Grace, 1993). Based on behavioral correlates, it has been shown that ~10% (43 out of 420 cells) of NAc neurons respond to a rewarding stimulus (Apicella et al., 1992; Schultz et al., 1992). Thus, a key question is that whether cocaine-induced generation of silent synapses occurs in all NAc neurons, or just in a subtype of NAc neurons. To detect potential differences in cocaine responses among these various neuronal subpopulations (e.g., responders vs. non-responders), we characterized the distribution of the electrophysiological parameters, including percentage of silent synapses (see methods for calculation) and decay time constant of NMDAR-mediated EPSC across the recorded NAc neurons. As shown in Fig. 2A, the NAc neurons from saline-treated rats (n = 53) exhibited a mono-modal pseudo-normal distribution with a median at ~0.1 (i.e., % of silent synapses is ~10%). On the other hand, NAc neurons from cocaine-treated rats (n = 47) also exhibited a mono-modal distribution with a median at ~0.4 (% of silent synapses is ~40%) (Fig. 2B). These results suggest that exposure to cocaine changed the median (of the portion of silent synapses) but not the distribution (e.g., no sign of bi-modal distribution was observed).
To further focus on a hypothetical situation in which ~50% of NAc neurons were the responders whereas the other ~50% were not, we built a hypothetical distribution model, in which we operationally defined the distribution of responders as that in Fig. 2B and the distribution of non-responders as that in Fig. 2A. We pooled the same number of cells (~45) from each group, and the pooled distribution exhibited a typical bi-modal property. This bi-modal distribution (solid curve) can be computationally peeled into two mono-modal distributions (dashed curves) corresponding to the distribution curves (Fig. 2C).
Thus, results from the above analyses suggest that it is likely that following cocaine exposure, silent/nascent synapses are generated across different neuronal subtypes in NAc neurons.
Cocaine-induced generation of silent synapses appears to arise from insertion of NR2B subunit-enriched NMDARs (Huang et al., 2009). We examined whether this increase is mediated by CREB activation. We first treated rats with a 5 day cocaine procedure and immunoprecipitated chromatin-bound activated CREB (phopho-CREB, pCREB) from NAc extracts. The levels of co-precipitated NR2A and NR2B promoter fragments were quantified by PCR and levels in cocaine treated animals were normalized to levels in saline treated control rats (Fig. 3A). Whereas the pCREB bound levels of NR2A promoter fragments did not change upon cocaine exposure, the pCREB bound levels of NR2B increased more than twofold, suggesting a specific activation of the NR2B gene by CREB (saline NR2A, 1.00 ± 0.16, n = 7 vs. cocaine NR2A, 1.25 ± 0.30, n = 6, p > 0.05; saline NR2B, 1.00 ± 0.16, n = 5 vs. cocaine NR2B, 3.46 ± 0.70, n = 6, p < 0.05).
We next tested whether CREB activation is sufficient for increasing NR2B protein levels. We incubated NAc tissue with sindbis viruses expressing caCREB or dnCREB and examined the protein level of NR2B subunits 24 h later. When normalized to the loading control actin, our results show that expression of caCREB increased total cellular NR2B protein abundance in the NAc more than threefold, whereas expression of dnCREB or GFP had little or no effect (relative level to uninfected tissues at total level: GFP 0.89 ± 020, n = 4; caCREB, 3.58 ± 0.56, n = 5; dnCREB, 1.10 ± 0.18, n = 5; F3, 16 = 11.6, p < 0.01, one-way ANOVA; p < 0.01 caCREB vs. uninf, GFP, or dnCREB, Bonferroni posttest; Fig. 3BC). Although the use of caCREB does not mimic the endogenous time course of CREB activation, the results show, that CREB activation by itself is sufficient to induce upregulation of NR2B.
A selective up-regulation of NR2B-containing NMDARs, as demonstrated here as well as in our previous study (Huang et al., 2009), would increase the relative portion of NR2B-containing NMDARs across all synaptic NMDARs. This scenario predicts that the overall (evoked) NMDAR EPSCs should be shaped by NR2B subunits. To test this prediction, we combined cocaine treatment with CREB manipulations and measured the time course of the decay of the NMDAR-mediated EPSC, a sensitive biophysical measurement of the relative contribution of synaptic NR2B-containing NMDARs, but may also reflect a small (< 15%) contribution of NR2D-containing NMDARs, known to be expressed in mouse striatum (Logan et al., 2007). Exposure to cocaine (the 2.5 cocaine procesure) induced a marked increase in the decay kinetics of NMDAR EPSCs (assessed by the time decayed from the peak to ½ peak amplitude, or T1/2) in NAc neurons (F5, 35 = 9.25, p < 0.01,one-way ANOVA; p < 0.05, uninf-saline vs. uninf-cocaine; Bonferroni posttest; n/m = 15/29 for uninf-saline and 5/9 for uninf-cocaine; Fig. 3D, E). Furthermore, this effect of cocaine was mimicked by expression of caCREB (p < 0.01 caCREB-saline vs. uninf-saline or GFP-saline, Bonferroni posttest; n/m = 6/11 for caCREB-saline and 5/10 for GFP-saline; Fig. 3D, E), and was prevented by expression of dnCREB (p < 0.05, dnCREB-cocaine vs. uninf-cocaine, p = 1.0, dnCREB-cocaine vs. uninf-saline or GFP-saline, Bonferroni posttest; n/m = 5/16 for dnCREB-cocaine; Fig. 3D, E).
Taken together, our results suggest that activation of CREB is required for the cocaine-induced increase in synaptic incorporation of NR2B-containing NMDARs and that activation of CREB alone is sufficient to trigger NR2B-containing receptors in NAc MSNs following exposure to cocaine.
Chronic exposure to cocaine increases the number of spines in NAc neurons, which persists for weeks after drug discontinuation (Kolb et al., 2003; Robinson and Kolb, 2004). We examined the number of dendritic spines in NAc neurons following the 5-day cocaine procedure used in our previous electrophysiology experiments (Huang et al., 2009). To quantify the spine density, we labeled neurons in lightly fixed tissues with the lipophilic fluorescent dye 1,1‘-diotadecyl-3,3,3’,3‘-tetramethylindocarbocyanine perchlorate (DiI) and examined dendritic spines using confocal microscopy. Two days after the 5-day cocaine protocol, the numbers of filopodia-like (t6 = −2.74, p < 0.05, t-test; n/m = 4/74 for saline and 4/55 for cocaine), mushroom-like protrusions (t6 = −2.89, p < 0.05, t-test), as well as the total (filopodia + mushroom) protrusions (t6 = −3.45, p < 0.05, t-test) on the distal dendrites of NAc MSNs were significantly increased (Fig. 4A, B). Furthermore, when pooling examined dendrites from all animals together, the spine densities in the cumulative plot exhibited a mono-modal distribution in saline-treated rats, and this mono-modal distribution was not altered by cocaine treatment (Fig. 4C). This distribution pattern suggests that dendrites within the NAc were evenly affected following exposure to cocaine, supporting our electrophysiological data on the mono-modal distribution of the effects on individual neurons (Fig. 2).
In agreement with the electrophysiological data, the increase in spine number after repeated cocaine administration was prevented by viral-mediated dnCREB expression compared to expression of GFP alone (F1, 13 = 19.70, p < 0.01, drug factor; F1, 13 = 13.59, p < 0.01, virus factor; F1, 13 = 19.11, p < 0.01, drug × virus, two-way ANOVA; p < 0.05, GFP-cocaine vs. GFP-saline, or dnCREB-saline, or dnCREB-cocaine, p > 0.05, dnCREB-cocaine vs. GFP-saline or dnCREB-saline, Tukey’s Multiple Comparison posttest; Fig. 4D, E). In these experiments, we counted spines visualized by the GFP fluorescence of the virally-transduced neurons according to published protocols (Russo et al., 2009; Maze et al., 2010). While this method reveals a smaller total number of spines compared to the DiI stained neurons, the relative increase caused by repeated cocaine administration is consistent between the two methods. In conclusion, these results indicate a requirement of CREB activation for the cocaine-induced increase in spine number.
Finally, we explored the potential role of silent synapses in cocaine-induced locomotor sensitization. Silent synapses are generated gradually during early exposure (3–4 days) over the 5-day cocaine procedure (Huang et al., 2009), with a time course similar to that of the development of cocaine-induced locomotor sensitization (Wolf, 1998; Kalivas, 2007; Kauer and Malenka, 2007). Without highly selective approaches to manipulate silent synapses, we focused on NR2B-containing NMDARs within the NAc. Our experimental design was to bilaterally and chronically perfuse the NR2B-selective antagonist Ro256981 into the NAc shell (Fig. 5A) through pre-implanted osmotic mini-pumps. As controls, rats with intra-NAc sham surgery and rats without surgery were tested with the 5-day cocaine procedure. Both control groups exhibited similar patterns of cocaine-induced locomotor sensitization upon repeated cocaine injections (control-cocaine × sham-cocaine: F1, 14 = 0.27, p = 0.95, two-way ANOVA; Fig. 5B) and similar basal locomotor activity upon saline injection (control-saline × sham-saline: F1, 12 = 0.28, p = 0.94, two-way ANOVA with repeated measure over day; Fig. 5B). This result indicates that the intra-NAc surgery did not affect basal and cocaine-induced locomotor responses. We next verified a concentration of Ro256981 that could effectively inhibit NR2B-containing NMDARs with minimal side effects. We examined the inhibitory effect of Ro256981 on NMDAR EPSCs at different concentrations and observed that at 20 – 200 nM, NMDAR EPSCs exhibited the first high affinity inhibition that was likely due to NR2B-selective inhibition (% inhibition: 2 nM, 4.7 ± 4.2, n = 3; 20 nM, 33.9 ± 5.5, n = 10; 200 nM, 35.4 ± 6.1, n = 6, Fig. 5C, D). One caveat of this pharmacological approach is that, if considered at the overall NMDAR level, application of 20 nM Ro256981 would cause a partial inhibition of total NMDARs by ~35%. This decrease in total NMDAR activity may produce some behavioral effect and thus complicate the interpretation of Ro256981-induced behavioral alteration. To address this potential caveat, we also verified that the non-selective NMDAR antagonist APV at the low concentration of 0.5 µM inhibited total NMDAR EPSCs to a similar degree (39.4 ± 6.2 %, n = 6) as 20 nM Ro256981 (Fig. 5D). However, application of APV did not alter the decay kinetics of NMDAR EPSCs as found for Ro256981 (% decrease in T1/2: 20 nM Ro256981, 11.2 ± 1.5, n = 10; 0.5 µM APV, 3.3 ± 2.5, n = 6, Fig. 5E). Thus, using APV at 0.5 µM in parallel with Ro256981 would allow us to clarify the potential behavioral alterations resulting from partial inhibition of total NMDARs.
We then examined the potential effects of intra-NAc perfusion of APV (500 nM) and Ro256981 (20 nM) on basal locomotor activity. Rats receiving intra-NAc perfusion of APV or Ro256981 exhibited similar basal activity in control rats or rats receiving sham surgery (p > 0.2 between any two groups, two-way ANOVA with repeated measure over day; Fig. 5F). To verify that Ro256981 via our intra-NAc perfusion setup could rapidly achieve maximal pharmacological and behavioral effects, we used the identical experimental procedure to perfuse the AMPAR-selective antagonist NBQX at a low concentration (1 µM). It has been demonstrated that inhibiting the NAc AMPARs prevents drug-induced locomotor response (Kaddis et al., 1993; Burns et al., 1994; Li et al., 1999; David et al., 2004). In the identical experimental setup that we used below, intra-NAc perfusion of a low concentration of NBQX blocked the cocaine-induced acute increases in locomotor activity (Fig. 5G). This result verifies that a brief preceding period (~15 min) of intra-NAc perfusion is sufficient for loaded pharmacological agents to achieve effective concentrations.
After verifying the experimental approaches, we started the core experiments. Shortly after the intra-NAc perfusion started, rats received one injection of saline (on day 0), followed by a 5-day cocaine regimen starting from day 1. A challenge injection of cocaine (12 mg/kg) was given on day 8, following a 2-day withdrawal period (day 6 to 7). The intra-NAc perfusion of Ro256981 (20 nM) or APV (0.5 µM) was applied from day -1 until day 8. Rats receiving only sham treatment developed steady cocaine-induced locomotor sensitization (p < 0.01, day 5 vs. day 1; p < 0.01, day 8 vs. day 1; Fig. 5H). The development of cocaine-induced locomotor sensitization was not affected by intra-NAc perfusion of a low concentration of APV (p < 0.01, day 5 vs. day 1; p < 0.01, day 8 vs. day 1; Fig. 5H). However, the development of locomotor sensitization was prevented by intra-NAc perfusion of Ro256981; indeed, in Ro256981-perfused rats, cocaine-induced locomotor activity decreased along the course of cocaine injection (p < 0.05, day 5 vs. day 1; p < 0.01, day 8 vs. day 1; F1, 12 = 14.22, p < 0.01 interaction between Ro-cocaine and Sham-cocaine, two-way ANOVA; Fig. 5H). This behavioral effect of Ro256981 was unlikely to be a general suppressive effect on locomotor activity because chronic intra-NAc perfusion of Ro256981 did not alter the basal locomotor activity in saline-treated rats (Fig. 5H).
We next examined whether intra-NAc inhibition of NR2B-containing NMDARs affected the expression of cocaine-induced locomotor sensitization. Rats with similar intra-NAc surgery were treated with the same 5-day cocaine procedure. On day 8, the intra-NAc perfusion of Ro256981 was turned on. Upon a challenge injection of cocaine (12 mg/kg), rats with intra-NAc perfusion of Ro256981 exhibited similar locomotor activities as observed in the sham controls (p > 0.5, t-test, Fig. 5I). Therefore, intra-NAc inhibition of NR2B-containing NMDARs and thus cocaine-generated silent synapses disrupted the development, but not the expression, of cocaine-induced locomotor sensitization. Taken together, these behavioral results suggest that NR2B-signaling, which is enriched in cocaine-generated silent synapses, is importantly implicated in addiction-related behaviors.
Silent synapses are highly efficient plasticity substrates that are primed to undergo both electrophysiological strengthening and anatomical growth to new synaptic activity (Isaac et al., 1999; Philpot and Zukin, 2010). A recent study by Ehlers and colleagues (Lee et al., 2010) in which the authors induce metaplasticity at single synapses provides compelling evidence that NR2B-containing NMDA receptors favor the induction of long-term potentiation, not long-term depression, an idea that has received considerable attention and been hotly debated (Morishita et al., 2007). Our current results together with previous findings strongly indicate a silent synapse-based linear pathway linking cocaine-induced molecular, cellular, and behavioral alterations: i) exposure to cocaine promotes phosphorylation and activation of CREB and recruitment of pCREB to the NR2B promoter in NAc neurons (Fig. 3) (Carlezon et al., 2005); ii) activation of CREB in NAc neurons increases NR2B protein levels (Fig. 3); iii) exposure to cocaine generates a large number of silent synapses, a process mediated by insertion of new, NR2B-containing NMDARs to likely new synaptic locations (Fig. 1 and and4)4) (Huang et al., 2009); iv) activation of CREB is necessary and sufficient to increase synaptic NR2B and to generate silent synapses in NAc neurons (Fig. 1 and and3);3); v) blockade of NR2B-containing NMDARs within the NAc, which preferentially inhibits cocaine-induced newly formed silent synapses (Huang et al., 2009), prevents the development of cocaine-induced locomotor sensitization (Fig. 5). Taken together, these results suggest a model whereby cocaine activates CREB, which promotes gene transcription of NR2B, leading to enhanced assembly and synaptic incorporation of NR2B-containing NMDARs and generation of silent synapses. The increase in silent synapses, in turn promotes cocaine-induced locomotor sensitization and is likely important to other addiction-related behaviors.
Silent synapses are typically considered to be immature glutamatergic synapses and as such have an increased capacity for recruiting functional AMPARs to strengthen glutamatergic synaptic transmission (Isaac et al., 1999; Liao et al., 1999; Groc et al., 2006; Kerchner and Nicoll, 2008). Cocaine increases the spine density in NAc MSNs as early as 24 h after the last cocaine injection (Fig. 4), consistent with findings of others (Kolb et al., 2003; Robinson and Kolb, 2004). This increase is at least partially mediated by de novo generation of silent synapses, as new NR2B-containing NMDARs are inserted into synapses and pharmacological inhibition of NR2B prevents the detection of silent synapses (Huang et al., 2009). However, in a recent study, Zito and colleagues demonstrated that in organotypic hippocampal slices, silent synapses are not correlated with the age of the spine (Zito et al., 2009). Thus it is possible that some of the silent synapses are generated from pre-existing synapses.
Cocaine-induced synapses, like immature synapses are enriched in the “immature”, NR2B-containing form of NMDARs (Dingledine et al., 1999; Washbourne et al., 2002; Groc et al., 2006; Lau and Zukin, 2007). This switch in NMDAR subunit composition is functionally important, as NR2B-containing NMDARs have longer decay time constants (Cull-Candy and Leszkiewicz, 2004) and are thus capable of integrating synaptic currents across broader time intervals. In addition to their longer currents, NR2B-containing NMDA receptors carry more Ca2+ current per unit charge (Sobczyk et al., 2005) and are preferentially tethered to the plasticity protein CaMKII (Barria and Malinow, 2005). In a recent paper Lee and collegues demonstratedthat the switch to NR2B-containing NMDARs primes synapses to more easily be potentiated (Lee et al., 2010). A potential fate of the silent synapses is to mature into fully functional synapses by recruiting new AMPARs. It is conceivable that the cocaine-induced silent synapses have a lowered threshold to undergo subsequent potentiation and thus prime the system for eliciting behavioral sensitization (Yao et al., 2004). If so, new, cocaine-specific synaptic connections and even cocaine-specific neural circuits might be formed accordingly.
Cocaine-induced silent synapses are generated in heterogenous populations of NAc neurons (Fig. 2). This finding is consistent with previous studies that demonstrate a decrease in the AMPAR/NMDAR ratio and increase in spine density in D1 and D2 dopamine receptor-containing MSNs in response to chronic cocaine administration (Lee et al., 2006b; Kourrich et al., 2007).
AMPAR/NMDAR ratios are decreased during short-term withdrawal but increased during long-term withdrawal (Kourrich et al., 2007). Findings in the present study suggest that an increase in NR2B and silent synapses contributes to the decrease in AMPAR/NMDAR ratio observed during short-term withdrawal whereas maturation of silent synapses thus recruiting new AMPARs contributes to the increase in AMPAR/NMDAR ratio observed during long-term withdrawal (Kourrich et al., 2007).
CREB plays an essential central role in the formation of new neural circuits during brain development (reviewed by Lonze and Ginty, 2002). Activation of CREB increases dendritic spine number, generates silent synapses, and facilitates the strengthening of existing synapses (Figs. 1, ,4)4) (Lonze and Ginty, 2002; Marie et al., 2005). dnCREB inhibits synaptogenesis (Lonze et al., 2002). In NAc, CREB is activated by exposure to cocaine or other drugs of abuse (reviewed by (Carlezon et al., 2005)). Upon activation, CREB is inducing the transcription of genes encoding synaptic vesicle proteins, postsynaptic scaffolding proteins, receptors, neurotrophins, and cell adhesion proteins, involved in synaptic and structural plasticity (McClung and Nestler, 2003; Renthal et al., 2009). Activation of CREB promotes the transcription of NR2B-containing NMDARs (Fig. 3). NMDAR subunits expressed in the NAc include NR1, NR2A, NR2B and NR2D (Monyer et al., 1994; Logan et al., 2007). Among these subunits, only the NR1 and NR2B genes contain cAMP response element (CRE) binding sites, such as activation of CREB increases NR1 and NR2B mRNA and protein (Klein et al., 1998; Dingledine et al., 1999). Our findings demonstrate that chronic cocaine promotes recruitment of CREB to the NR2B gene promoter, driving NR2B transcription and enhanced translation and assembly of NR2B-containing NMDARs. Furthermore, we show that cocaine-induced generation of silent synapses is mediated by CREB. Thus activation of CREB is an initial step deployed by cocaine to promote generation of new synapses and neurocircuits.
It has long been known that NMDARs are essential for the development of cocaine-induced locomotor sensitization (Wolf, 1998). Our results extend these findings by showing that the NR2B-containing NMDARs are essential for the development of cocaine-induced locomotor sensitization (Fig. 5). Because NR2B-containing NMDARs are enriched in cocaine-generated silent synapses (Huang et al., 2009), an attractive scenario is that the NR2B-signaling contributes to cocaine-induced behavioral sensitization. This interpretation is supported by our finding that inhibition of NR2B-containing NMDARs during cocaine administration attenuates locomotor sensitization. In contrast, a subunit-nonselective NMDAR antagonist D-APV did not block the development of sensitization when applied at concentrations that inhibited a similar portion of total NMDARs as Ro256981 (Fig. 5). As the number of silent synapses decreases after several days of withdrawal from cocaine, while the locomotor sensitization persists, it is conceivable that these premature synapses engage in the formation of new circuitsthat form the structural basis for locomotor sensitization (Robinson and Berridge, 2000; Huang et al., 2009). This circuitry-based interpretation is in unison with the view that a circuitry, rather than a specific brain site, mediates the development of locomotor sensitization (reviewed by Wolf, 1998). Alternatively, it is also possible that the overall intra-NAc NR2B-signaling, rather than the new synaptic connections, is required for the development of locomotor sensitization. Thus, the insertion of NR2B-containing NMDARs in silent synapses can be regarded as an overall enhancement of the NAc NR2B-signaling, which, in turn, may trigger NR2B-specific neuronal modifications that are important for the development of locomotor sensitization.
A likely cellular consequence of cocaine-generated silent synapses is the extension of the functionally active state (i.e., upstate) of NAc neurons. Synaptic NMDARs preferentially contribute to the duration of the upstates of NAc MSNs because of their relatively slow decay kinetics, which has been demonstrated both computationally (Wolf et al., 2005) and experimentally (Huang et al., 2007). Thus, an overall increase in postsynaptic NMDARs may prolong the duration of individual upstates in NAc neurons. This effect can be further enhanced by NR2B-containing receptors, which exhibit even slower decay kinetics, and are upregulated by cocaine (Huang et al., 2009). A second cellular consequence is the enhancement of NR2B-calcium calmodulin kinase II (CaMKII)-signaling. NR2B-containing NMDARs possess higher binding affinity for CaMKII than NR2A-containing receptors, and thus result in stronger stimulation of the NMDAR/CaMKII-signaling cascade upon activation (Strack and Colbran, 1998). The activity of CaMKII in NAc is upregulated following exposure to cocaine (Anderson et al., 2008), and inhibiting the NAc CaMKII attenuates cocaine-induced locomotor sensitization (Pierce et al., 1998) and drug-seeking behaviors (Anderson et al., 2008).
The behavioral and functional consequences of cocaine and amphetamine administration are very similar (Wolf, 1998). However, the effect on NR2B expression and the effect of the NR2B antagonist Ro256981 on the expression of drug-induced locomotor sensitization are seemingly different between cocaine and amphetamine (Fig. 5) (Huang et al., 2009; Mao et al., 2009). Prolonged withdrawal from amphetamine causes a reduction in total NR2B protein levels and high concentrations of Ro256981 increased locomotor-sensitization after 14 days of withdrawal. In addition to the potential pharmacological and physiological difference between cocaine and amphetamine, different experimental design (time course) and pharmacological manipulations (concentrations of Ro256981) may account for the differences in the results.
Taken together, this study suggests that newly formed synapses contribute to the development of cocaine-induced locomotor sensitization, thus potentially providing a molecular angle to manipulate the neural bases underlying sensitized emotional and motivational state associated with addiction.
The work was supported by the Humboldt Foundation (YD), an IRSF fellowship (YL), the Alcohol and Drug Abuse Research Program of Washington State, the Hope Foundation for Depression Research, and by NIH DA023206, NIH DA01433, and NIH DA08227. Cocaine was supplied in part by the Drug Supply Program of NIH NIDA. The European Neuroscience Institute is jointly funded by the University Medicine Göttingen and the Max Planck Society.