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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Am Coll Cardiol. Author manuscript; available in PMC 2013 February 21.
Published in final edited form as:
PMCID: PMC3285410
NIHMSID: NIHMS351813

Catheter-deliverable hydrogel derived from decellularized ventricular extracellular matrix increases endogenous cardiomyocytes and preserves cardiac function post-myocardial infarction

Abstract

Objectives

This study evaluated the use of an injectable hydrogel derived from ventricular extracellular matrix (ECM) for treating myocardial infarction (MI) and its ability to be delivered percutaneously.

Background

Injectable materials offer promising alternatives to treat MI. While most of the examined materials have shown preserved or improved cardiac function in small animal models, none have been specifically designed for the heart and few have translated to catheter delivery in large animal models.

Methods

We have developed a myocardial specific hydrogel, derived from decellularized ventricular ECM, which self-assembles when injected in vivo. Female Sprague-Dawley rats underwent ischemia reperfusion followed by injection of the hydrogel or saline 2 weeks later. The implantation response was assessed via histology and immunohistochemistry, and potential for arrhythmogenesis was examined using programmed electrical stimulation 1 week post-injection. Cardiac function was analyzed with magnetic resonance imaging 1 week pre-injection and 4 weeks post-MI. In a porcine model, we delivered the hydrogel using the NOGA guided Myostar catheter, and utilized histology to assess retention of the material.

Results

We demonstrate that injection of the material in the rat MI model increases endogenous cardiomyocytes in the infarct area and maintains cardiac function without inducing arrhythmias. Furthermore, we demonstrate feasibility of transendocardial catheter injection in a porcine model.

Conclusion

To our knowledge, this is the first in situ gelling material to be delivered via transendocardial injection in a large animal model, a critical step towards the translation of injectable materials for treating myocardial infarction in humans. Our results warrant further study of this material in a large animal model of myocardial infarction and suggest this may be a promising new therapy for treating myocardial infarction.

Keywords: myocardial infarction, heart failure, biomaterial, scaffold, extracellular matrix

Introduction

Cardiovascular disease continues to be the leading cause of death in the United States (US), as well as the rest of the western world, with an estimated 785,000 new myocardial infarctions (MI) each year (1). Post-MI pathological changes are often progressive, consisting of an initial inflammatory phase, followed by the upregulation of matrix metalloproteinases that degrade the extracellular matrix (ECM), leading to infarct expansion and wall thinning, and eventual collagen scar deposition to resist deformation and rupture. The resultant negative left ventricular (LV) remodeling is thought to independently contribute to progressive deterioration of cardiac function leading to HF post-MI (2). When end stage failure occurs, heart transplantation or implantation of an LV assist device are the only available treatments. Therefore, the development of new therapies is necessary.

One of the first alternatives to heart transplantation was a technique termed cellular cardiomyoplasty. This technique consists of injecting cells, suspended in saline or cell culture medium, into the recipient’s myocardium (3-9). This is an attractive approach because it allows for minimally invasive intramyocardial delivery through a catheter. While many clinical trials using cellular cardiomyoplasty have shown some promise (3,6-9), cell retention, engraftment, and survival have been difficult to achieve, due in part to the lack of an appropriate extracellular microenvironment (10). Thus, approaches using cardiac tissue engineering have begun utilizing synthetic and natural biomaterials as scaffolds to improve transplanted cell survival (11), or as stand alone acellular scaffolds to replace the degraded cardiac ECM, preserve or improve cardiac function, and attenuate LV remodeling (12-15). Injectable biomaterials are particularly attractive since they have the potential to be delivered using a minimally invasive approach, and a material-only therapy would eliminate many of the complications associated with cell therapies.

We have developed a myocardial matrix hydrogel, derived from decellularized ventricular porcine ECM, which is the first cardiac-specific injectable material, offering a replacement scaffold that mimics the native cardiac extracellular environment (16). The myocardium was first decellularized using a perfusion based technique to generate an intact three dimensional scaffold (17). In contrast, we process the ventricular ECM into an injectable liquid that self-assembles upon injection in vivo to form a nanofibrous and porous scaffold. This material offers a biochemical and structural composition that mimics the native ventricular ECM. Herein, we tested the safety and efficacy of injecting the myocardial matrix hydrogel in a rat myocardial infarction model. We demonstrate that injection of myocardial matrix increases endogenous cardiomyocyte in the infarct area and preserves cardiac function post-MI, without increasing incidences of arrhythmia. Considering many biomaterials being explored as cardiac therapies in small animal models do not have the appropriate properties to translate to catheter delivery in the heart (18), we also tested the ability of the myocardial matrix hydrogel to be delivered via catheter in a porcine model. We demonstrate that the myocardial matrix can be delivered to the myocardium via a percutaneous transendocardial approach, thus paving the way towards clinical translation of a new minimally invasive, biomaterial-based therapy for MI.

Methods

Myocardial matrix preparation

The myocardial matrix was decellularized and prepared as previously described (16). Briefly, Yorkshire farm pigs (35-45 kg) were euthanized using an overdose of Pentobarbital (90 mg/kg), administered intravenously, and their hearts removed. The ventricular tissue was isolated and cut into small rectangular pieces, rinsed in phosphate buffered saline (PBS), and decellularized using 1% sodium dodecyl sulfate (SDS), until the ECM was white. Decellularized ECM was fresh frozen in Tissue Tek O.C.T., sectioned into 5 μm slices, and stained with H&E to confirm decellularization. The decellularized ECM was then rinsed with DI water overnight, lyophilized, and milled into a fine powder. The powder was then solubilized by enzymatic digestion using pepsin and 0.1M HCl for at least 54 hours prior to use, as modified from a previously published protocol (19). The solubilized myocardial matrix was adjusted to pH 7.4 with NaOH, on ice, and brought to 6 mg/mL for injection. A Blyscan assay was used to confirm sulfated glycosaminoglycan content (16). For catheter compatibility tests in the porcine model and for identification at 1 week post-injection in the rat model, the material was biotin labeled. For biotin labeling, a 10 mM solution of EZ link Sulfo-NHS-Biotin (Pierce, Rockford, IL) was prepared and mixed with the solubilized myocardial matrix for a final concentration of 0.3 mg biotin/mg matrix. The mixture was allowed to sit on ice for two hours prior to use.

Liquid Chromatography Mass Spectrometry

Liquid Chromatography Mass Spectrometry (LC-MS/MS) was used to analyze the myocardial matrix, to ensure retained protein content. Lyophilized powder samples were digested in trypsin, in preparation for LC-MS/MS. Electrospray ionization experiments were run on a QSTAR-Elite hybrid mass spectrometer (AB/MDS Sciex) interfaced to a reversed-phase high-pressure liquid chromatograph. The column used was a 10 cm-180 ID glass capillary packed with 5-μm C18 Zorbax™ beads (Agilent Technologies, Santa Clara, CA). Peptides were eluted from the C18 column into the mass spectrometer using a linear gradient of 5-80% Buffer B (100% acetonitrile, 0.2% formic acid, and 0.005% trifluoroacetic acid) over 60 min at 400 μl/min. (Buffer A was composed of 98% H2O, 2% acetonitrile, 0.2% formic acid, and 0.005% trifluoroacetic acid). LC-MS/MS data were acquired in a data-dependent fashion, time-of-flight MS were acquired at m/z 400 to 1600 Da, MS/MS data were acquired from m/z 50 to 2,000 Da. Once collected, peptide identifications were based on at least one peptide with the confidence of above 99% for that peptide identification, using Protein Pilot 2.0 (Life Technologies Inc, Carlsbad, CA).

Rat MI and injection surgical procedures

All experiments in this study were performed in accordance with the guidelines established by the Committee on Animal Research at the University of California, San Diego and the American Association for Accreditation of Laboratory Animal Care. Animal numbers used in each experimental subset are reported in Table 1. MI was induced via 25 minute ischemia-reperfusion surgery on female Sprague Dawley rats (225 - 250 g). Animals were anesthetized with 5% isoflurane, intubated, and maintained at 2.5% isoflurane. A left thoracotomy (20) was performed to allow access to the heart, the pericardial sac removed, and a single 6-0 silk suture was placed into the myocardium, around the left coronary artery. The 6-0 suture was tied in a loop, knotted at the end of the loop, a 3-0 silk suture was tied over the knot, and then the 3-0 suture was pulled through the 6″ PE90 tubing to occlude the artery for 25 mins. Ischemia-reperfusion for as little as 17 minutes with this method of occlusion has been shown to cause consistent infarcts (21,22). After 25 minutes, the tubing was released, and the suture was removed, allowing the vessel to reperfuse. The animal was then sutured and allowed to recover. Two weeks post-MI, rats were randomized to ensure a similar distribution of EF in each group based on baseline MRI measurements, and injected with 75 μL of either saline or solubilized myocardial matrix at 6 mg/mL following a procedure previously described (13,16,22). Briefly, an incision was made in the abdomen, and the diaphragm was cut to expose the heart. A single injection of material was delivered through a 30 G needle into the LV wall, and was confirmed by a lightening of the tissue. After injection, the abdomen was stitched closed following suction of the chest cavity, and animals were allowed to recover. For both surgical procedures, animals were given 0.05 mg/kg of buprenorphine hydrochloride (Reckitt Benckiser Healthcare (UK) Ltd., Hull, England), an analgesic, prior to recovery from anesthesia. Animals were also given 3 cc of Lactated Ringers (Hospira Inc. Lake Forest, IL) solution for hydration during surgery.

Table 1
Animal numbers

Programmed electrical stimulation

To test arrhythmia inducibility, programmed electrical stimulation was performed on hearts, one-week post-injection. Rats were anesthetized, as previously described, and surgical access to the heart was gained. A pacing electrode was inserted gently into the viable region of the left ventricle (LV) wall, such that the exposed electrode tip came in contact with the interior of the LV wall, and the necessary voltage was determined, using a fixed rate pacing (pulse width of 1ms, cycle length of 120ms), which was increased until pacing was induced, and voltage was then doubled for the rest of the pacing protocol. ECG was recorded throughout the pacing procedure, using an ECG signal recorder (DATAQ di-148), for one full hour. Burst pacing and extra-stimulus testing were induced, according to methods previously developed (23-25). After each pacing test, the ECG was observed for any induced arrhythmias; incidences of ventricular tachycardia (VT) were recorded. At the end of the entire burst pacing and extra-stimulus pacing procedures for the rat, the ECG recording was stopped and saved. The heart was excised and fresh frozen for histological analysis.

Magnetic resonance imaging

Cine-MRI was performed on a 7T Bruker magnet, at the UC San Diego fMRI center, using an electrocardiogram (ECG) triggered fast slow angle shot (FLASH) gradient echo pulse sequence. The following parameters were used: flip angle = 15°, echo time = 1.28 ms, repetition time = 7.7 ms, data matrix = 256 × 128, field of view = 50 mm2, slice thickness = 1 mm, and 25 phases were collected per cardiac cycle. Scanning parameters and methods were modified from those determined effective to evaluate ventricular geometry in murine models (26-28). Briefly, rats were anesthetized with isoflurane, while monitoring heart rate and respiratory rate. The long axis of the heart was identified, and subsequent contiguous short axis slices were acquired throughout the cardiac cycle from apex of the heart to the base of the heart. Images were acquired using a gating system, so that the first image corresponds to end-diastole. Image J was used to manually outline the endocardial surface for each slice corresponding to end-diastolic (ED) and end-systolic (ES), for the calculation of ventricular area. Although true ES occurs upon opening of the mitral valve and can be detected in echocardiography images (29), for consistency of image analysis, ES is identified as minimal LV lumen area. Each area was then multiplied by slice thickness (1 mm) to calculate LV volume. EDV and ESV were calculated, allowing for the calculation of ejection fraction (EF). From the volume obtained at ED and ES, EF (%) was calculated to be ((EDV-ESV)/EDV)*100%. At one-week post-MI, MR images were taken for evaluation of baseline, pre-injection parameters. At six weeks post-MI (four weeks post-injection), hearts were again assessed as described above. EF and LV geometry were compared pre and post-injection therapy, thus allowing each animal to serve as its own internal control. Healthy animals were also imaged, revealing an EF of 74 ± 5% for our model. Rats that did not present an EF representative of an infarct (< 69%, one standard deviation from a healthy animal) at one-week were eliminated from the study.

Catheter delivery in a porcine model

Biotin labeled myocardial matrix was tested for clinical feasibility within a porcine model using a Myostar Intramyocardial Injection device for transendocardial delivery, as developed for minimally invasive cellular transplantation (4,5). Two Yucatan mini pigs (28 - 40 kg) were used for the study. For one animal, pre-anesthethesia, Telazol (5 mg/kg) was administered, followed by anesthetics propofol (2.4 mg/kg), until effect, and atrophine (0.02 mg/kg). The animal was intubated, and ventilated with 1 - 2.5% isoflurane and 1 L/min oxygen. An 8-F arterial sheath was inserted through the right femoral artery for access to the LV. Coronary angiography was performed for visualization of the arteries, and ECG was monitored throughout the surgery. Myocardial matrix was biotin labeled prior to injection for histological post-operative identification, and kept on ice until time of injection. Prior to injection, a unipolar electromechanical map (NOGA) was created of the endocardial wall and used to guide injection. The injection, through a 27 G retractable needle, followed protocols commonly used for cellular delivery (4,5,9). Here, a 1 mL luer lock syringe, was loaded with myocardial matrix and attached to the Myostar catheter for transendocardial injection. The NOGA map was used for identification of injection locations, and twenty-five 0.2 mL injections were performed throughout the LV free wall and septal wall. In the second animal, following anesthesia, a MI was given via deployment of two embolization coils as previously described (5,30). Two weeks later, an injection procedure was performed with the NOGA guided Myostar catheter as described above. Fifteen 0.25 mL injections were performed in the infarct and borderzone.

Histology and immunohistochemistry

For the rat studies, animals were euthanized with an overdose of sodium pentobarbital (200 mg/kg). Hearts were immediately removed, fresh frozen in Tissue Tek O.C.T. freezing medium, and sectioned into 10 μm slices. Slides spaced approximately every 0.5 mm were stained with H&E for identification of infarcted tissue or for examination of inflammatory response by a pathologist blinded to the study. Immunohistochemistry (IHC) was performed on five slides from the infarct in each heart using the antibodies directed against the following antigens: Ki67 (Abcam, Cambridge, MA; 1:100), cardiac specific Troponin-T (NeoMarkers, Fremont, CA; 1:50), Connexin43 (Millipore, Temecula, CA; 1:200), alpha smooth muscle actin (Dako, Carpinteria, CA; 1:75), anti-CD163 (AbD Serotec, Raleigh, NC; 1:50), anti-c-Kit (Santa Cruz Biotechnology, Santa Cruz, CA; 1:100). All primary antibodies were visualized by the addition of Alexa Fluor 568 and 488 (Invitrogen) secondary antibodies. Sections that were stained with only the primary antibody or only the secondary antibody were used as negative controls. All sections stained with Hoecsht to visualize nuclei, and mounted with Fluoromount (Sigma). Images were taken with a Carl Zeiss Observer D.1 and analysis was performed by a blinded investigator with AxioVision software and Photoshop. To assess retention and biodistribution of the myocardial matrix upon injection in a porcine model, the heart and satellite organs were removed for histological analysis. The pigs remained on anesthesia for 1-2 hours following the injection surgery, before being euthanized with Fatal Plus at 0.22 mL/kg. This time frame was determined in the rat model to be adequate time to allow for gelation of the material within the myocardial tissue (16). Upon sacrifice, the heart was removed, with pericardium intact. Heart slices were fresh frozen using O.C.T. Tissue Tek for histological analysis, to locate the myocardial matrix. Five to ten grams of each of the following organs were collected to assess distribution to satellite organs as previously described for cell retention following catheter delivery (31,32): right and left lung, liver, spleen, right and left kidney, and right and left brain. Samples were frozen for histological analysis. The ventricular tissue and satellite organs were each sectioned into 10 μm sections and stained with H&E. Adjacent sections were stained for visualization of biotin labeled myocardial matrix. Slides were fixed in acetone 1.5 min, incubated with superblock buffer (30 min), followed by 3% hydrogen peroxide (30 min), and horseradish peroxidase conjugated neutravidin (5 μg/mL, 30 min) at room temperature. The reaction was visualized by incubation with diaminobenzidine (DAB) for ten minutes. Sections from each organ were stained at the same time as the ventricular tissue, and images taken after ten minutes of incubation.

Statistical analysis

Data are presented as mean ± standard error of the mean. MRI data pre- and post-treatment was assessed for each group using a paired t-test. Changes in EF, ESV, and EDV, and IHC data were compared with a two sample t-test. Significance was accepted at p < 0.05.

Results

Injectable myocardial matrix fabrication and characterization

We isolated ventricular ECM from fresh porcine ventricular myocardium (Fig. 1A) using sodium dodecyl sulfate (SDS) as previously described (16). After approximately 4-5 days in 1% SDS, cellular material was effectively removed, yielding white, translucent ventricular ECM (Fig. 1B). H&E sections of the decellularized ECM confirmed cell removal and lack of nuclei (Fig. 1C). The cardiac ECM was then lyophilized and milled into a powder (Fig. 1D), and finally solubilized with enzymatic digestion (Fig. 1E), thereby allowing for injection in subsequent studies (Fig. 1F).

Figure 1
Myocardial matrix fabrication

To confirm the decellularized myocardial ECM retained the proteins and proteoglycans native to the myocardium, myocardial matrix powder was characterized using Liquid Chromatography Mass Spectrometry (LC-MS/MS). LC-MS/MS revealed a variety of ECM proteins, indicating retained protein content after decellularization. The ECM proteins, glycoproteins, and proteoglycans identified include: collagen types I, III, IV, V, and VI, elastin, fibrinogen, lumican, perlecan, fibulin, and laminin. Collagen, laminin, and elastin are the major ECM proteins, responsible for structure. Perlecan, a heparin sulfate proteoglycan associated with vascularized tissues, is known to promote adhesion and basic fibroblast growth factor-receptor binding, as well as play a role in developmental and remodeling processes, such as angiogenesis (33-35). Fibulin, a calcium-binding glycoprotein, commonly associates with other ECM components such as fibronectin (36,37). The identification of these components within the decellularized and processed myocardial matrix indicates a retained complex combination of proteins and proteoglycans, important for a biomimetic tissue engineering scaffold. It should, however, be noted that mass spectrometry is not an all-inclusive technique and, therefore, other proteins may not have been identified.

Arrhythmia inducibility

Numerous materials have been injected into infarcted rodent myocardium to date, yet no studies have directly assessed safety in terms of a potential for arrhythmias, which has been a concern with cell injections (3,38-40). We therefore assessed arrhythmia inducibility using programmed electrical stimulation in vivo by burst and extra-stimulus pacing protocols in rats one-week post-injection of myocardial matrix (n=20) compared to saline (n=16). We chose to examine one-week post-injection as the material is still present in the tissue at this time. In vivo pacing protocols (23-25) induced non-sustained ventricular tachycardia (monomorphic or polymorphic, self-terminating VT) in 37.5% of saline injected hearts and 35% of matrix injected hearts. Sustained VT (>30 seconds) was induced once during burst pacing in a matrix injected rat (5% occurrence rate), and once during extra-stimulus pacing in a saline injected rat (6.25% occurrence rate). No statistical significance was found when comparing the average incidence of VT between groups (p = 0.8) (Fig. 2).

Figure 2
Programmed electrical stimulation to assess potential for arrhythmogenesis

Histological and immunohistochemical analysis

To examine the local tissue response to myocardial matrix injection, a subset of animals utilized in the arrhythmia studies were utilized for histological and immunohistochemical analysis (n=5 each group). Histological assessment of infarcts one-week post-injection showed an increased cellular infiltration response in myocardial matrix injection animals. There was a moderate mononuclear cell infiltration response with predominantly lymphocytes and some macrophages (Fig. 3A). Additional spindle shaped cells were also observed, likely indicating some fibroblast infiltration. There was no indication of matrix encapsulation or rejection. We further performed immunohistochemistry on the heart sections with antibodies for markers for cardiomyocytes (Troponin), M2 macrophages (CD163), and proliferative cells (Ki67). Striking differences were observed in the size of cardiomyocyte islands surviving within the infarct region in matrix injected hearts, and we thus quantified the area of these regions, as compared to controls. The average area of these islands of viable myocardium within the infarct of matrix-injected hearts (0.05 ± 0.01 mm2) was statistically larger than the average area of those within the infarct of saline-injected hearts (0.03 ± 0.01 mm2, matrix, p = 0.045, Fig. 3B). Within these regions of viable myocardium, positive staining for Connexin 43 was observed (not shown), indicating the presence of gap junctions. While quantification of M2 macrophages within the infarct was not significantly significant between groups (p = 0.2) (Fig. 3C), matrix injected hearts did show a significantly higher density of proliferative cells (saline 27.6 ± 6.8 per mm2, matrix 75.1 ± 18 per mm2, p = 0.039) (Fig. 3D). Many of the Ki67+ cells had a rounded/oval nuclei shape, which is indicative of lymphocytes. Co-staining of Ki67 and Troponin revealed that only ~1% of Ki67 positive cells were proliferating cardiomyocytes. Additional slides, co-stained for Ki67 and α-smooth muscle actin, identified that myofibroblasts were proliferative within matrix-injected hearts, but were not quantified, due to low numbers. We also examined matrix-injected regions for the presence of c-kit+ cells, which are progenitor cells that have been shown to differentiate into myocardial precursors and vascular cells in the heart (41,42). C-kit+ cells were observed within the myocardial matrix scaffold (Fig. 3E); however, they were in low numbers.

Figure 3
Histological and immunohistochemical assessment

Preservation of cardiac function post-myocardial infarction

Magnetic resonance imaging (Fig. 4A-D) was used to measure LV end-diastolic volume (EDV), end-systolic volume (ESV), and ejection fraction (EF) at 1 week post-MI (1 week prior to injection) and 6 weeks post-MI (4 weeks post-injection) to evaluate effects of myocardial matrix injections on cardiac function (Table 2). As expected, the EF (Fig. 4E) of saline injected hearts (n=6) significantly declined, while both the ESV (Fig. 4F) and EDV (Fig. 4G) significantly expanded. In contrast, there was no statistical significance between EF, ESV, and EDV of myocardial matrix injected animals (n=6) (Fig. 4E-G) despite injections being performed 1 week after baseline imaging. When comparing the percent change in EF, ESV, and EDV between groups, myocardial matrix injected animals had a an increase in EF (Fig. 4B), and a relative decrease in percent change in ESV (Fig. 4I) and EDV (Fig. 4J) compared to controls, although these were not significant.

Figure 4
MRI analysis in rat myocardial infarction model
Table 2
MRI Data

Percutaneous transendocardial delivery of myocardial matrix

The liquid myocardial matrix was tested for clinical feasibility using a Myostar Intramyocardial Injection device for transendocardial delivery, a technique that has been employed for minimally invasive delivery for cellular cardiomyoplasty (4,5,9). Prior to injection, a unipolar electromechanical map (NOGA) was created to allow for selection of injection sites. Matrix was prepared as described above, loaded into a 1 mL syringe for connection to the catheter (Fig. 5A) and injections were made via endoventricular catheter delivery using the Myostar Intramyocardial needle-injection catheter. Successful transendocardial delivery of liquid myocardial matrix into 25 injection sites (0.2 mL each site) throughout the septal wall and LV free wall was achieved in the healthy animal, while 15 injections (0.25 cc each site) were achieved in the infarct and borderzone of the infarcted animal, with no sustained arrhythmias during or after the injection procedures. The material remained injectable during the approximately hour-long procedures, without premature gelation or clogging of the catheter. The location of each individual injection was guided and documented through the use of NOGA mapping (Fig. 5B). One-two hours after injection of myocardial matrix via transendocardial catheter delivery, the animals were euthanized and the hearts removed. By gross observation, the hearts appeared healthy with no signs of pericardial effusion (Fig. 5C). Detection of the matrix within the LV free wall (Fig. 5D,E) and septal wall of the healthy animal and within the infarct area in the MI animal (Fig. 5F), confirmed successful delivery into the myocardium, as well as gelation of the matrix in vivo. As with any intramyocardial injection technique, there is a concern that the injected material will leak into the ventricle and travel through the blood stream to other organs. Thus, the lungs, kidney, brain, liver, and spleen were also examined; no myocardial matrix was observed in any of the satellite organs.

Figure 5
Percutaneous transendocardial delivery of myocardial matrix hydrogel

Discussion

This work establishes proof-of-concept for the clinical feasibility of the recently developed myocardial matrix as an injectable biomaterial for treating myocardial infarction through a minimally invasive approach. Herein, we show that this injectable scaffold preserves cardiac function in a small animal model. We further demonstrate that the material can be successfully delivered via a percutaneous transendocardial approach into the ventricular wall in both healthy and infarcted porcine myocardium.

While developing a therapy from porcine tissue helps to eliminate the need for human organ donation, there is potential concern associated with the immunogenicity of a xenogeneic material. Previous studies have however shown that xenogeneic decellularized ECMs are biocompatible, and many have been approved by the FDA and used successfully in the clinic (43). We nonetheless examined the local tissue response to the porcine derived myocardial matrix hydrogel. The mononuclear infiltrate seen here is similar to that seen in previous studies which show the immune response of decellularized small intestine submucosa (SIS) ECM as being comparable to syngeneic muscle implants, rather than eliciting foreign body giant cell formation and encapsulation, indicative of rejection seen with xenogeneic implantation (44,45). Furthermore, small intestine submucosa ECM implantation resulted in an increase of cytokines that are known to be produced by a subset of lymphocytes, Th2 cells, which are associated with a normal immune response and graft acceptance (46,47). Thus, the presence of lymphocytes within the infarcted tissue of the myocardial matrix injected hearts corroborates that the myocardial matrix is able to elicit an immune response typical of decellularized matrices, and suggests a potentially positive impact on the infarct environment, as the Th2 response that predominates with decellularized ECMs results in the production of anti-inflammatory cytokines (44,45). We also found an increase in proliferating cells, suggesting the matrix affects the proliferation of cells within the infarct, as was also seen upon injection of a small intestine submucosa emulsion (48). While many of the cells were lymphocytes, we also co-stained for cardiomyocytes and myofibroblasts. The heart is known to have limited regenerative capacity, yet cardiomyocytes have been shown to proliferate in the failing heart (49). However, only ~1% of Ki67+ cells co-stained for Troponin. A small subset of proliferating cells were also identified as myofibroblasts, which are thought to be critical in the remodeling process post-MI (50). We further observed the presence of c-kit+ cells within the matrix scaffold; however, they were in low numbers.

A variety of materials have been explored as injectable therapies for cardiac repair post-MI, and shown preserved or improved cardiac function (12-14,51). However, the recently developed myocardial matrix is naturally derived from decellularized porcine ventricular tissue and is therefore uniquely able to offer properties of the native cardiac ECM. A material that mimics the microenvironment of native ECM structurally and biochemically is important in any tissue engineering application, as it allows for appropriate cell-matrix interactions (52,53). Natively, these interactions are specific for each tissue, with each ECM having its own distinct combination of proteins and proteoglycans (52,54). The myocardial matrix has been shown, through gel electrophoresis and glycosaminoglycan quantification (16), as well as mass-spectrometry analysis, to retain a complexity of ventricular ECM proteins, peptides, and polysaccharides, making it a cardiac specific scaffold for myocardial tissue engineering. This tissue specificity may provide benefits over non-tissue matched decellularized matrices. For example, a previous study utilizing decellularized urinary bladder matrix as a ventricular patch lead to the presence of cartilaginous tissue (55), which may have been the result of non-tissue specific cues.

Here we also show an increase in the size of cardiomyocyte islands surviving in the infarct area, which has, to the best of our knowledge, not been previously reported with other material injections. While most cardiomyocytes undergo apoptosis or necrosis soon after the induction of MI and cell death peaks 4 days post-MI, additional waves of CM death continue for weeks or months (56). Thus, the retained areas of CMs suggest that matrix injection may act to salvage remaining CMs, or prevent the further necrosis. Furthermore, gap junctions, identified by Connexin 43 staining, were present among these cells, suggesting electrical conductance within the CM clusters. In addition to local effects on endogenous cell types, a cardiac specific scaffold may also have key benefits for transplant cell delivery as the biochemical cues in the myocardial matrix have been recently shown in vitro to promote enhanced maturation of human embryonic stem cell derived cardiomyocytes (57).

We demonstrate that injection of the myocardial matrix hydrogel preserves cardiac function post-MI in a rodent model; however, the exact mode of action behind the improvement for this hydrogel as well as other injectable materials has not yet been fully elucidated (58). A recent study in our lab, which showed that an inert nondegradable synthetic material did not affect cardiac function post-MI, suggests that biological cues or cell infiltration as a result of material degradation may play important roles in preserving/improving LV function (59). In this study, we show the myocardial matrix results in an influx of cells typical of decellularized ECM materials, which includes lymphocytes. As stated above a Th2 response, as seen with decellularized ECMs, results in the production of anti-inflammatory cytokines, including IL-10 (44,45). IL-10 has been shown to inhibit LV remodeling and improve function post-MI (60), and therefore may also be a potential mode of action here. We have previously shown that the myocardial specific biochemical cues in the myocardial matrix promote maturation of human embryonic stem cell derived cardiomyocytes in vitro (57). In this study, we saw an increase in the size of surviving islands of viable myocardium within the infarct. This result may be due to the presence of myocardial specific cues in the myocardial matrix; however, the material on its own does not appear to results in significant regeneration as we only saw low numbers of c-kit+ cells within the scaffold. While a material alone therapy would facilitate translation, a combination therapy may be needed to promote regeneration.

A safety issue that has arisen with intramyocardial injections is the potential for arrhythmogenesis. While many studies have examined injectable materials for treating MI in rodent models, none have directly assessed whether injection of these materials increases arrhythmias. We therefore performed programmed electrical stimulation studies one week post-injection in the rat model as well as monitored the EKG up to 2 hours post-injection in the porcine model. We did not observe an increase in incidences of arrhythmia with injection of the myocardial matrix hydrogel. Our results, along with two recent reports of material injections in porcine models (through intramyocardial injection (15) and intracoronary infusion (61)), suggest that material delivery into the infarct area does not lead to induced arrhythmogenesis. However, material degradation and scar maturation over time may lead to electrophysiological changes, and, therefore, it will be critical to assess multiple time points for a given material in a large animal model prior to clinical translation.

Several materials investigated for injectable therapy, including fibrin glue (13,21), collagen (14), alginate (12), chitosan (62), Matrigel (63), and hyaluronic acid (64) have demonstrated preserved or improved cardiac function, yet may not translate to catheter delivery in the heart, which would limit their relevance for minimally invasive treatment in humans. For an injectable material to be clinically relevant for percutaneous delivery in the heart, the material must remain liquid, with an appropriate viscosity for delivery of multiple injections through a catheter, and convert to a gel once it is within the myocardial tissue. While several materials have shown promising results in small animals, few have actually been translated to percutaneous delivery in large animals. Recently, alginate (15) was delivered through intracoronary injection in large animals. However, endocardial delivery, commonly used for cellular cardiomyoplasty (4,5,9,15,65,66), is considered to be the preferred method of catheter delivery in terms of retention (67-69), as it allows for direct intramyocardial delivery and does not require access to the coronary vessels. In addition, the guarantee of migration into the myocardium is uncertain with intracoronary delivery, and there is a risk of microembolism (70). Here we show that the myocardial matrix hydrogel preserves cardiac function post-MI, and also demonstrate that it is deliverable via catheter. The myocardial matrix was successfully injected via a percutaneous, transendocardial approach in both healthy and infarcted myocardium without clogging the catheter. It should, however, be noted that some leakage of injectate into the ventricle is known to occur with transendocardial delivery (31,32). Any material that leaks into the ventricle would likely be rapidly diluted in the blood, thereby preventing gelation. We confirmed through histological analysis that the myocardial matrix formed a hydrogel within the porcine myocardial tissue without measurable embolization in other organs. It is, however, possible that a small amount of material was present in other organs and below our detection limit.

There are a few limitations to this study. One limitation is that injections were performed two weeks post-MI, an entire week after the baseline MRI analysis. Because of this delay between baseline measurements and treatment injection, the LV volume likely continued to increase prior to injection, which is indicated by the data (Fig. 4). Therefore, it is unknown whether this would remain had injections been performed immediately post baseline imaging. Secondly, LV remodeling is well underway at this injection time point, and injection of the myocardial matrix shortly after MI, at which time the native ECM has been recently degraded, may have enhanced effects. We however chose to inject at a later time point as it is more clinically relevant than injection immediately post-MI where there is a significant risk of ventricular rupture. Lastly, while we examined catheter delivery in a large animal, the majority of this study is in a rat myocardial infarction model. Inherent limitations with this model include the inability to precisely control injections and therefore the inability to homogenously cover the entire infarct area. Examining functional changes in a large animal MI model will be critical prior to clinical translation.

While a variety of materials have shown success in small animal models (12-14,51), the myocardial matrix not only preserves cardiac function in a rat model, but also shows initial translation to large animals. Specifically, we have shown clinical feasibility of the myocardial matrix for minimally invasive delivery, by demonstrating catheter delivery and retention within the myocardium. The myocardial matrix is the first material to be designed as a tissue specific, injectable scaffold for cardiac tissue engineering, and is to our knowledge the first in situ gelling material to be injected via percutaneous transendocardial delivery. Our results warrant further study of this material in a large animal MI model and demonstrate potential of this technology for treating patients suffering from MI.

Acknowledgements

We thank Carolina Rogers, Dr. Majid Ghassemian, and Dr. Kent Osborne for assistance with porcine tissue collection, mass spectrometry, and histological assessment, respectively. In addition, we would like to acknowledge Timothy Salazar and Dr. Miriam Scadeng of the fMRI center, as well as Dr. Joyce Chuang for MRI guidance. We also thank Dr. Adam Wright and Aboli Rane for assistance with pacing studies, and Biologics Delivery Systems for providing NOGA mapping support.

Funding Sources:This research was supported in part by the NIH Director’s New Innovator Award Program, part of the NIH Roadmap for Medical Research, through grant number 1-DP2-OD004309, the Walter H. Coulter Foundation (Miami, FL), and NSF 1014429.

Abbreviations and Acronyms

ECM
extracellular matrix
MI
myocardial infarction
HF
heart failure
LV
left ventricle
PBS
phosphate buffered saline
SDS
sodium dodecyl sulfate
LC-MS/MS
liquid chromatography mass spectrometry
VT
ventricular tachycardia
ECG
electrocardiogram
EDV
end-diastolic volume
ESV
end-systolic volume
EF
ejection fraction
DAB
diaminobenzidine

Footnotes

Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Relationship with industry: Drs. Christman and Dib hold equity interest in Ventrix, Inc. Dr. DeMaria is on the scientific advisory board of Ventrix, Inc. Mr. Salvatore and Dr. Kinsey are employees of Ventrix, Inc.

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